Endoplasmic reticulum, Bcl-2 and Ca2+ handling in apoptosis.

abstract：:Mathematical models are often used to elucidate mechanisms behind cytosolic Ca2+ oscillations. We have evaluated the use of mathematical modelling to analyse and quantify Ca2+ signal patterns, in single, adherent human neutrophils (PMN) after stimulation by the bacterial peptide N-formyl-methionyl-leucyl-phenylalanine...

journal_title：Cell calcium

authors： Røtnes JS,Røttingen JA

更新日期：1994-06-01 00:00:00

abstract：:Middle East Respiratory Syndrome coronavirus (MERS-CoV) infections are associated with a significant mortality rate, and existing drugs show poor efficacy. Identifying novel targets/pathways required for MERS infectivity is therefore important for developing novel therapeutics. As an enveloped virus, translocation thr...

journal_title：Cell calcium

authors： Gunaratne GS,Yang Y,Li F,Walseth TF,Marchant JS

更新日期：2018-11-01 00:00:00

abstract：:Glucose induces large amplitude oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i) in pancreatic beta-cells. The effects of temperature on these oscillations were examined by monitoring [Ca2+]i continuously in single beta-cells from ob/ob-mice using dual wavelength microfluorometry. The oscillations of [Ca2+...

journal_title：Cell calcium

authors： Hellman B,Gylfe E,Grapengiesser E,Panten U,Schwanstecher C,Heipel C

更新日期：1990-06-01 00:00:00

abstract：:Numerous lines of evidence indicate that nuclear calcium concentration ([Ca2+]n) may be controlled independently from cytosolic events by a local machinery. In particular, the perinuclear space between the inner nuclear membrane (INM) and the outer nuclear membrane (ONM) of the nuclear envelope (NE) likely serves as a...

journal_title：Cell calcium

authors： Secondo A,Petrozziello T,Tedeschi V,Boscia F,Pannaccione A,Molinaro P,Annunziato L

更新日期：2020-03-01 00:00:00

abstract：:TRPM family (Transient receptor potential channels, M for melastatin) is a group of intrinsic plasma membrane ion channels which are widely expressed throughout human body. It has been identified as a potent entry point of working desperate diseases out in a new way with newfangled ideas and safer technological means....

journal_title：Cell calcium

authors： Chen Y,Zhang X,Yang T,Bi R,Huang Z,Ding H,Li J,Zhang J

更新日期：2019-05-01 00:00:00

abstract：:Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane...

journal_title：Cell calcium

authors： Morgan BP,Luzio JP,Campbell AK

更新日期：1986-12-01 00:00:00

abstract：:Intercellular Ca(2+)-signaling, after mechanical stimulation of calf pulmonary artery endothelial cells (CPAE), was investigated with fluorescence video imaging. Mechanical stimulation evoked an intracellular Ca(2+)-response in the mechanically stimulated (MS) cell, proceeding to the neighboring (NB) cells as a Ca(2+)...

journal_title：Cell calcium

authors： Moerenhout M,Himpens B,Vereecke J

更新日期：2001-02-01 00:00:00

abstract：:STIM and Orai isoforms orchestrate store operated Ca2+ entry (SOCE) and thus cytosolic Ca2+ fluctuations following stimulation by hormones, growth factors and further mediators. Orai1 is a target of Nedd4-2, an ubiquitin ligase preparing several plasma membrane proteins for degradation. Phosphorylation of Nedd4-2 by t...

journal_title：Cell calcium

authors： Lang F,Eylenstein A,Shumilina E

更新日期：2012-11-01 00:00:00

abstract：:When Xenopus oocytes injected with rat brain poly(A)+RNA were voltage-clamped in a recording solution containing Ca2+, a depolarization pulse induced a transient current, ICl(Ca), which reflects calmodulin-mediated opening of endogenous Cl- channels in response to a Ca2+ influx through Ca2+ channels of brain origin. I...

journal_title：Cell calcium

authors： Kaneko S,Doi E,Watanabe H,Nomura Y

更新日期：1990-04-01 00:00:00

abstract：:The annexins are a family of calcium-binding proteins. Data from protein and cDNA sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. The proteins are pres...

journal_title：Cell calcium

authors： Burgoyne RD,Geisow MJ

更新日期：1989-01-01 00:00:00

abstract：:Katz and co-workers showed that Ca(2+) triggers exocytosis. The existence of sub-micrometer domains of greater than 100 microM [Ca(2+)](i) was postulated on theoretical grounds. Using a modified, low-affinity aequorin, Llinas et al. were the first to demonstrate the existence of Ca(2+) 'microdomains' in squid presynap...

journal_title：Cell calcium

authors： Oheim M,Kirchhoff F,Stühmer W

更新日期：2006-11-01 00:00:00

abstract：:An initial model has been proposed to describe a mechanism for cytosolic calcium oscillations [Jafri MS. Vajda S. Pasik P. Gillo B. (1992) A membrane model for cytosolic calcium oscillations: a study using Xenopus oocytes. Biophys. J., 63, 235-246]. In this paper we extend our original model to include the effects of ...

journal_title：Cell calcium

authors： Jafri MS,Gillo B

更新日期：1994-07-01 00:00:00

abstract：:The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...

journal_title：Cell calcium

authors： De Marchi E,Bonora M,Giorgi C,Pinton P

更新日期：2014-07-01 00:00:00

abstract：:Palmitoylation (S-acylation) is the reversible conjugation of a fatty acid (usually C16 palmitate) to intracellular cysteine residues of proteins via a thioester linkage. Palmitoylation anchors intracellular regions of proteins to membranes because the palmitoylated cysteine is recruited to the lipid bilayer. NCX1 is ...

journal_title：Cell calcium

authors： Gök C,Fuller W

更新日期：2020-03-01 00:00:00

abstract：:In smooth muscle, Ca(2+) regulates cell division, growth and cell death as well as providing the main trigger for contraction. Ion channels provide the major access route to elevate the cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) in smooth muscle by permitting Ca(2+) entry across the plasma membrane and release of ...

journal_title：Cell calcium

authors： Chalmers S,Olson ML,MacMillan D,Rainbow RD,McCarron JG

更新日期：2007-10-01 00:00:00

abstract：:In HEK293 cells, transfected with the Ca2+ channel protein TRPV6, Ca2+ influx is increased and TRPV6 is tyrosine phosphorylated following addition of the tyrosine phosphatase inhibitor N,N-dimethyl-hydroxamido hydroxovanadate to cells. This effect of DMHV is enhanced by co-transfection of cells with the tyrosine kinas...

journal_title：Cell calcium

authors： Sternfeld L,Anderie I,Schmid A,Al-Shaldi H,Krause E,Magg T,Schreiner D,Hofer HW,Schulz I

更新日期：2007-07-01 00:00:00

abstract：:Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...

journal_title：Cell calcium

authors： Fliegert R,Hölzer HT,Guse AH

更新日期：2018-12-01 00:00:00

abstract：:A 106 kD protein was isolated from skeletal sarcoplasmic reticulum (SR) vesicles and shown to have the properties of SR Ca2+ release channels, including blockade by 5 nM ryanodine. In view of extensive reports that the ryanodine-receptor complex consists of four 565 kD junctional feet proteins (JFPs) and is the 'physi...

journal_title：Cell calcium

authors： Salama G,Nigam M,Shome K,Finkel MS,Lagenaur C,Zaidi NF

更新日期：1992-11-01 00:00:00

abstract：:Receptor-activated cytoplasmic calcium (Ca2+) oscillations have been investigated in single pancreatic acinar cells by microfluorimetry (Fura-2 as indicator). At submaximal concentrations of the agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8), both give rise to oscillatory changes in the cytosolic...

journal_title：Cell calcium

authors： Yule DI,Lawrie AM,Gallacher DV

更新日期：1991-02-01 00:00:00

abstract：:We have used single cell fluorescence imaging techniques to examine how functional properties of the caffeine-sensitive Ca(2+) store change during differentiation of a sub-population of caffeine-sensitive SH-SY5Y cells. Application of caffeine (30 mM) 1-10.5 min after a 'priming' depolarisation pulse of 55 mM K(+) rev...

journal_title：Cell calcium

authors： Riddoch FC,Brown AM,Rowbotham SE,Redfern CP,Cheek TR

更新日期：2007-03-01 00:00:00

abstract：:We describe experiments in which the low affinity indicator Oregon Green BAPTA 5N was used to record the spatially resolved changes in [Ca(2+)] from intracellular stores in rat gastric myocytes. Cells were loaded with the membrane permeant form of the indicator and imaged using a confocal scanning laser microscope. In...

journal_title：Cell calcium

authors： White C,McGeown G

更新日期：2002-04-01 00:00:00

abstract：:To determine intracellular Ca2+ concentrations more accurately, we examined Kd of Fura-2 for Ca2+ in conditions which were systemically changed. In a solution comprising of 150 mM KCI, 20 mM MOPS-KOH, pH 6.94, 60-100 microM EGTA and 1 microM Fura-2, Kd at 20 degrees C was 0.266 +/- 0.016 microM (mean +/- SEM) (21 dete...

journal_title：Cell calcium

authors： Uto A,Arai H,Ogawa Y

更新日期：1991-01-01 00:00:00

abstract：:The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...

journal_title：Cell calcium

authors： Bihler I,Charles P,Sawh PC

更新日期：1982-08-01 00:00:00

abstract：:Human neutrophils, preloaded with the fluorescent probe, Fura-2, were exposed to Ca2+-releasing agents. The monitored traces of fluorescence were transformed by computer to cytosolic Ca2+ concentration ([ Ca2+]i). Due to quenching of Fura-2, the addition of Mn2+ enabled us to compute the cytosolic concentration of tot...

journal_title：Cell calcium

authors： Foder B,Scharff O,Thastrup O

更新日期：1989-10-01 00:00:00

abstract：:The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...

journal_title：Cell calcium

authors： Oishi H,Budel S,Schuster A,Stergiopulos N,Meister JJ,Bény JL

更新日期：2001-10-01 00:00:00

abstract：:Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brai...

journal_title：Cell calcium

authors： Cayce JM,Bouchard MB,Chernov MM,Chen BR,Grosberg LE,Jansen ED,Hillman EM,Mahadevan-Jansen A

更新日期：2014-04-01 00:00:00

abstract：:Aging is a complex process that differentially impacts multiple cognitive, sensory, neuronal and molecular processes. Technological innovations now allow for parallel investigation of neuronal circuit function, structure and molecular composition in the brain of awake behaving adult mice. Thus, mice have become a crit...

journal_title：Cell calcium

authors： Radulescu CI,Cerar V,Haslehurst P,Kopanitsa M,Barnes SJ

更新日期：2021-01-23 00:00:00

abstract：:Ca(2+) signals control DNA synthesis and repair, gene transcription, and other cell functions that occur within the nucleus. The nuclear envelope can store Ca(2+) and release it into the nucleus via either the inositol 1,4,5-trisphosphate receptor (InsP3R) or the ryanodine receptor (RyR). Furthermore, many cell types ...

journal_title：Cell calcium

authors： Marius P,Guerra MT,Nathanson MH,Ehrlich BE,Leite MF

更新日期：2006-01-01 00:00:00

abstract：:IP3R is located to the inner nuclear membrane. Nuclear IP3R is recognized as a 220 kD immunoreactive protein by antisera raised against purified rat brain IP3R. Antisera against C-terminal 95-108 peptide fragment derived from rat brain IP3R does not reveal immunoreactivity in the nucleus. Nuclear IP3R is sensitive to ...

journal_title：Cell calcium

authors： Malviya AN

更新日期：1994-10-01 00:00:00

abstract：:Here, we report the characterization of a human cDNA coding for the recently published amino acid sequence of a calcium-binding S100 protein, S100A12 (CGRP, calgranulin C, CAAF1, p6). The exon/intron structure of the S100A12 gene is similar to most other S100 genes. It is composed of three exons which are divided by t...

journal_title：Cell calcium

authors： Wicki R,Marenholz I,Mischke D,Schäfer BW,Heizmann CW

更新日期：1996-12-01 00:00:00