Abstract：
:Glucose induces large amplitude oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i) in pancreatic beta-cells. The effects of temperature on these oscillations were examined by monitoring [Ca2+]i continuously in single beta-cells from ob/ob-mice using dual wavelength microfluorometry. The oscillations of [Ca2+]i disappeared when the temperature was increased above 42 degrees C and were reversibly inhibited below 30 degrees C. However, cooling did not prevent a glucose response in terms of the average rise of [Ca2+]i. Since patch clamp studies of single beta-cells have indicated a random occurrence of glucose-induced action potentials at room temperature, it was important to explore how the sugar affected the electrical activity at 37 degrees C. Using the cell-attached configuration of the patch clamp technique for such analyses, the action potentials were found to occur in bursts with durations similar to the large amplitude oscillations of [Ca2+]i.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Hellman B,Gylfe E,Grapengiesser E,Panten U,Schwanstecher C,Heipel Cdoi
10.1016/0143-4160(90)90053-wsubject
Has Abstractpub_date
1990-06-01 00:00:00pages
413-8issue
6eissn
0143-4160issn
1532-1991pii
0143-4160(90)90053-Wjournal_volume
11pub_type
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