Abstract：
:Increases in global Ca(2+) in the endothelium are a crucial step in releasing relaxing factors to modulate arterial tone. In the present study we investigated spontaneous Ca(2+) events in endothelial cells, and the contribution of smooth muscle cells to these Ca(2+) events, in pressurized rat mesenteric resistance arteries. Spontaneous Ca(2+) events were observed under resting conditions in 34% of cells. These Ca(2+) events were absent in arteries preincubated with either cyclopiazonic acid or U-73122, but were unaffected by ryanodine or nicotinamide. Stimulation of smooth muscle cell depolarization and contraction with either phenylephrine or high concentrations of KCl significantly increased the frequency of endothelial cell Ca(2+) events. The putative gap junction uncouplers carbenoxolone and 18alpha-glycyrrhetinic acid each inhibited spontaneous and evoked Ca(2+) events, and the movement of calcein from endothelial to smooth muscle cells. In addition, spontaneous Ca(2+) events were diminished by nifedipine, lowering extracellular Ca(2+) levels, or by blockers of non-selective Ca(2+) influx pathways. These findings suggest that in pressurized rat mesenteric arteries, spontaneous Ca(2+) events in the endothelial cells appear to originate from endoplasmic reticulum IP(3) receptors, and are subject to regulation by surrounding smooth muscle cells via myoendothelial gap junctions, even under basal conditions.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Kansui Y,Garland CJ,Dora KAdoi
10.1016/j.ceca.2007.11.012subject
Has Abstractpub_date
2008-08-01 00:00:00pages
135-46issue
2eissn
0143-4160issn
1532-1991pii
S0143-4160(07)00183-2journal_volume
44pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:The sphingosine derivatives sphingosylphosphorylcholine (SPC) and sphingosine-1-phosphate (S1P) caused a similar elevation of the intracellular Ca2+ concentration ([Ca2+]i) in an immortalized airway epithelial cell line (CFNP9o-) incubated in Ca(2+)-free medium. The maximal effect was obtained with 2 microM SPC and 0....
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90095-1
更新日期：1998-06-01 00:00:00
abstract：:Extracellular nucleotides are potent Ca2+ mobilizing agents. A variety of receptors for extracellular ATP are recognised. Some are involved in fast neuronal transmission and operate as ligand-gated ion channels. Others are involved in the paracrine or autocrine modulation of cell function. Many receptors of this type ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90080-2
更新日期：1995-02-01 00:00:00
abstract：:The Bcl-2 protein, best known for its ability to inhibit apoptosis, interacts with the inositol 1,4,5-trisphosphate receptor (IP(3)R) Ca(2+) channel to regulate IP(3)-mediated Ca(2+) release from the endoplasmic reticulum. This review summarizes the current state of knowledge regarding the interaction of Bcl-2, and al...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.05.011
更新日期：2011-09-01 00:00:00
abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00143-x
更新日期：2003-10-01 00:00:00
abstract：:Evidence is accumulating on a key role of T-type channels in neurotransmitter release. Recent works have brought undisputable proofs that T-type channels are capable of controlling hormone and neurotransmitters release in association with exocytosis of large dense-core and synaptic vesicles. T-type channel-secretion c...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.019
更新日期：2006-08-01 00:00:00
abstract：:NAADP has been shown to act as a second messenger in a wide range of systems from plants to mammalian cells. Although it had always been considered as a canonical second messenger, recent work has shown that it is also active when applied extracellularly. It has also been suggested that NAADP might have a direct actio...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.10.002
更新日期：2007-06-01 00:00:00
abstract：:C3H/10T1/2 mouse embryo fibroblasts stimulated by a steady electric field (10 V/cm) for 30 min exhibited lamellar retraction on the sides facing the electrodes. Some cells elongated and preferentially oriented with their long axis perpendicular to the field direction. Depletion of external calcium or blockage of calci...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(85)90012-0
更新日期：1985-06-01 00:00:00
abstract：:We have used single cell fluorescence imaging techniques to examine how functional properties of the caffeine-sensitive Ca(2+) store change during differentiation of a sub-population of caffeine-sensitive SH-SY5Y cells. Application of caffeine (30 mM) 1-10.5 min after a 'priming' depolarisation pulse of 55 mM K(+) rev...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.06.007
更新日期：2007-03-01 00:00:00
abstract：:The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(82)90004-5
更新日期：1982-08-01 00:00:00
abstract：:The peptide of HIV-1 envelope gp41 (a.a 628-683), referred to herein as P5, contains P1, a conserved galactose-specific lectin domain for binding the mucosal HIV-1-receptor, galactosyl ceramide (GalCer), as shown earlier, and a potential calcium-binding site (a.a 628-648). P1 contains contiguous epitopes recognized by...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.04.011
更新日期：2008-01-01 00:00:00
abstract：:A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90027-6
更新日期：1995-10-01 00:00:00
abstract：:In addition to its role in artery contraction, Rho kinase (ROCK) is reported to be involved in the Ca(2+) response to vasoconstrictor agonist in rat aorta. However the signaling pathway mediated by ROCK had not been investigated so far and it was not known whether ROCK also contributed to Ca(2+) signaling in cultured ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.07.002
更新日期：2012-12-01 00:00:00
abstract：:Maitotoxin (MTX), a water-soluble polyether obtained from the marine dinoflagellate Gambierdiscus toxicus increased intracellular calcium in a concentration-dependent manner in fibroblasts obtained from human skin. The effect of this toxin was both saturable and of high affinity, showing an apparent half activation co...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90087-7
更新日期：1997-07-01 00:00:00
abstract：:We present a new method for ratiometric Ca2+ measurements using indicators with excitation spectra in the visible range of wavelengths. Laser-scanning confocal microscopy was used to record intracellular Ca(2+)-signals with high temporal and spatial resolution in single cardiac myocytes. The patch-clamp technique was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90040-d
更新日期：1993-05-01 00:00:00
abstract：:We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-medi...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.01.002
更新日期：2014-03-01 00:00:00
abstract：:Specific sequences (cis-acting elements) in the 3'-untranslated region (UTR) of RNA, together with stabilizing and destabilizing proteins (trans-acting factors), determine the mRNA stability, and consequently, the level of expression of several proteins. Such interactions were discovered initially for short-lived mRNA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.004
更新日期：2006-10-01 00:00:00
abstract：:Pancreatic islets produce pulses of insulin and other hormones that maintain normal glucose homeostasis. These micro-organs possess exquisite glucose-sensing capabilities, allowing for precise changes in pulsatile insulin secretion in response to small changes in glucose. When communication among these cells is disrup...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.102081
更新日期：2019-11-01 00:00:00
abstract：:Calcium and redox signals are presently established as essential regulators of many cellular processes. Nevertheless, we are still far from fully understanding the physiological and pathological importance of these universal second messengers. It is becoming increasingly apparent that many cellular functions are not r...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.04.008
更新日期：2016-08-01 00:00:00
abstract：:There is controversy as to whether TRP channels participate in mediating store-operated current (I(SOC)) and store-operated Ca(2+) entry (SOCE). Our recent study has demonstrated that TRPC1 forms heteromeric channels with TRPV4 in vascular endothelial cells and that Ca(2+) store depletion enhances the vesicle traffick...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.08.006
更新日期：2011-12-01 00:00:00
abstract：:The calcium release channel/ryanodine receptor of rabbit skeletal muscle sarcoplasmic reticulum is tightly associated with the immunophilin FK-506 binding protein (FKBP-12). The immunosuppressant drug FK-506 effectively dissociates FKBP-12 from the calcium release channel of terminal cisternae (TC) vesicles. Furthermo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90048-5
更新日期：1994-02-01 00:00:00
abstract：:Pancreatitis is an increasingly common disease that carries a significant mortality and which lacks specific therapy. Pathological calcium signalling is an important contributor to the initiating cell injury, caused by or acting through mitochondrial inhibition. A principal effect of disordered cell signalling and imp...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2007.11.013
更新日期：2008-07-01 00:00:00
abstract：:The properties of the Ca2+, Mg2+-ATPase of erythrocyte membranes from patients with cystic fibrosis (CF) were extensively compared to that of healthy controls. Following removal of an endogenous membrane inhibitor of the ATPase, activation of the enzyme by Ca2+, calmodulin, limited tryptic digestion or oleic acid, as ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(85)90010-7
更新日期：1985-06-01 00:00:00
abstract：:The effect of the uterotonic pituitary hormone oxytocin on the regulation of intracellular calcium concentration ([Ca2+]i) was studied in single cells of a smooth muscle cell line derived from human non-pregnant myometrium. [Ca2+]i was measured with fluorescence microscopy, and by recording the activity of Ca(2+)-acti...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90049-4
更新日期：1996-07-01 00:00:00
abstract：:We investigated whether the expression of human alpha-parvalbumin affects depolarization-induced elevations of the cytosolic free calcium concentration ([Ca2+]i) in human neuroblastoma SKNBE2 cells. A full length human parvalbumin cDNA was cloned by PCR from human cerebellum and transiently transfected into SKNBE2 cel...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90062-7
更新日期：1996-06-01 00:00:00
abstract：:In murine ventricular myocytes, activation of mechanosensitive ion channels (MSCs) includes activation of non-selective cation currents and deactivation of inwardly rectifying potassium currents. Using pharmacological inhibitors and knockout models, we analyzed signaling steps that are critical to transduce the mechan...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.06.002
更新日期：2009-01-01 00:00:00
abstract：:Photolysis of caged calcium (Nitr5, Calbiochem) can be used to study calcium dependent processes such as excitation-contraction coupling and muscular mechanics. Expensive high energy light sources are routinely used for UV light exposure, but this study describes an alternative low cost xenon flash unit constructed in...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90017-5
更新日期：1994-05-01 00:00:00
abstract：:Changes in cytosolic free Ca(2+) concentration ([Ca(2+)]i) were monitored optically in hair cells mechanically isolated from frog semicircular canals using the membrane-impermeant form of the Ca(2+)-selective dye Oregon Green 488 BAPTA-1 (OG, 100 microM). Cells stimulated by depolarization under whole-cell voltage cla...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2001.0220
更新日期：2001-08-01 00:00:00
abstract：:Hepatocytes stimulated with calcium-mobilising agonists generate free Ca transients whose frequency is modulated by hormone concentration. Importantly, the time-course of individual free Ca transients is independent of agonist dose but does change with agonist species. A receptor-controlled model in which protein kina...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(91)90011-3
更新日期：1991-02-01 00:00:00
abstract：:Ca2+ pumps are essential for removing cytosolic Ca2+ either across the plasma membrane (PM) or into internal organelles such as the sarcoplasmic reticulum (SR). Four genes (PMCA1, PMCA2, PMCA3 and PMCA4) have been reported to encode the PM Ca2+ pumps and three (SERCA1, SERCA2 and SERCA3) to encode the SR Ca2+ pumps. T...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(92)90025-n
更新日期：1992-01-01 00:00:00
abstract：:Cardiogenesis depends on a tightly regulated balance between proliferation and differentiation of cardiac progenitor cells (CPCs) and their cardiomyocyte descendants. While exposure of early mouse embryos to Ca(2+) channel antagonists has been associated with abnormal cardiac morphogenesis, less is known about the con...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.02.018
更新日期：2014-05-01 00:00:00
