Intracellular Ca(2+) oscillations induced by over-expressed Ca(V)3.1 T-type Ca(2+) channels in NG108-15 cells.

abstract：:Photolysis of caged calcium (Nitr5, Calbiochem) can be used to study calcium dependent processes such as excitation-contraction coupling and muscular mechanics. Expensive high energy light sources are routinely used for UV light exposure, but this study describes an alternative low cost xenon flash unit constructed in...

journal_title：Cell calcium

authors： Van Koeveringe GA,Van Mastrigt R

更新日期：1994-05-01 00:00:00

abstract：:Ca(2+) influx through voltage-gated Ca(2+) channels triggers the release of neurotransmitters at presynaptic terminals. Some sensory receptor cells in the peripheral auditory and visual systems have specialized synapses that express an electron-dense organelle called a synaptic ribbon. Like conventional synapses, ribb...

journal_title：Cell calcium

authors： Cho S,von Gersdorff H

更新日期：2012-09-01 00:00:00

abstract：:STIM and Orai isoforms orchestrate store operated Ca2+ entry (SOCE) and thus cytosolic Ca2+ fluctuations following stimulation by hormones, growth factors and further mediators. Orai1 is a target of Nedd4-2, an ubiquitin ligase preparing several plasma membrane proteins for degradation. Phosphorylation of Nedd4-2 by t...

journal_title：Cell calcium

authors： Lang F,Eylenstein A,Shumilina E

更新日期：2012-11-01 00:00:00

abstract：:Using whole cell patch clamp recordings on unfertilized eggs of the ascidian Ciona intestinalis, we are able to detect ryanodine receptors within the oocytes. Our approach is based on measurements of the voltage-activated inward calcium currents. Two types of Ca2+ currents have been described on the oocyte membrane of...

journal_title：Cell calcium

authors： Arnoult C,Albrieux M,Antoine AF,Grunwald D,Marty I,Villaz M

更新日期：1997-02-01 00:00:00

abstract：:The annexins are a family of calcium-binding proteins. Data from protein and cDNA sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. The proteins are pres...

journal_title：Cell calcium

authors： Burgoyne RD,Geisow MJ

更新日期：1989-01-01 00:00:00

abstract：:The measurement of intracellular calcium ion concentrations [( Ca2+]i) in single living cells using quantitative fluorescence microscopy draws from a diverse set of disciplines, including cellular biology, optical physics, statistics and computer science. Over the last few years, we have devised and built a number of ...

journal_title：Cell calcium

authors： Moore ED,Becker PL,Fogarty KE,Williams DA,Fay FS

更新日期：1990-02-01 00:00:00

abstract：:An inward current responsible for hormone regulated Ca2+ entry has been identified in cultured rat hepatocytes using whole cell patch clamp. Addition of 20 nM vasopressin or of 100 microM ATP induced the inward current, which could be observed more clearly after blocking an outward K+ current. This large outward K+ cu...

journal_title：Cell calcium

authors： Duszynski J,Elensky M,Cheung JY,Tillotson DL,LaNoue KF

更新日期：1995-07-01 00:00:00

abstract：:Adipokinetic hormone (AKH)-induced mobilization of insect fat body glycogen occurs through activation of glycogen phosphorylase. In the migratory locust, signal transduction of AKH-I, -II and -III has been shown to involve the formation of cAMP. In the present study, we show that both the elevation of fat body cAMP le...

journal_title：Cell calcium

authors： Vroemen SF,Van Marrewijk WJ,Schepers CC,Van der Horst DJ

更新日期：1995-06-01 00:00:00

abstract：:The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...

journal_title：Cell calcium

authors： Oishi H,Budel S,Schuster A,Stergiopulos N,Meister JJ,Bény JL

更新日期：2001-10-01 00:00:00

abstract：:The strength of the heart beat depends on the amplitude and time course of the transient increase in [Ca(2+)] in the myocytes with each cycle. [Na(+)](i) modulates cardiac contraction through its effect on the Ca(2+) flux through the Na/Ca exchanger. Cardiac excitation-contraction coupling has been postulated to occur...

journal_title：Cell calcium

authors： Verdonck F,Mubagwa K,Sipido KR

更新日期：2004-06-01 00:00:00

abstract：:In mammals Ca2+ concentration in the extracellular fluids ([Ca2+]o) is essential for a number of vital processes varying from bone mineralization to blood coagulation, regulation of enzymatic processes, modulation of permeability and excitability of plasma membranes. For this reason [Ca2+]o is under strict control of ...

journal_title：Cell calcium

authors： Riccardi D

更新日期：1999-09-01 00:00:00

abstract：:While genetically encoded Ca(2+) indicators (GECIs) allow Ca(2+) imaging in model organisms, the gene expression is often under the control of a single promoter that may drive expression beyond, the cell types of interest. To enable more cell-type specific targeting, GECIs can be brought under the, control of the inte...

journal_title：Cell calcium

authors： Wong SS,Kotera I,Mills E,Suzuki H,Truong K

更新日期：2012-01-01 00:00:00

abstract：:Ca(2+) is known as a universal messenger mediating a wide variety of cellular processes, including cell death. In fact, this ion has been proposed as the 'cell death master', not only at the intracellular but also at the intercellular level. The most direct form of intercellular spread of cell death is mediated by gap...

journal_title：Cell calcium

authors： Decrock E,Vinken M,Bol M,D'Herde K,Rogiers V,Vandenabeele P,Krysko DV,Bultynck G,Leybaert L

更新日期：2011-09-01 00:00:00

abstract：:NMDA receptors (NMDARs) are glutamate-gated ion channels involved in excitatory synaptic transmission and in others physiological processes such as synaptic plasticity and development. The overload of Ca2+ ions through NMDARs, caused by an excessive activation of receptors, leads to excitotoxic neuronal cell death. Fo...

journal_title：Cell calcium

authors： Plutino S,Sciaccaluga M,Fucile S

更新日期：2019-06-01 00:00:00

abstract：:One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid met...

journal_title：Cell calcium

authors： Várnai P,Gulyás G,Tóth DJ,Sohn M,Sengupta N,Balla T

更新日期：2017-06-01 00:00:00

abstract：:Mammalian homologues of the Drosophila transient receptor potential channel (TRPC) are involved in Ca(2+) entry following agonist stimulation of nonexcitable cells. Seven mammalian TRPCs have been cloned but their mechanisms of activation and/or regulation are still the subject of intense research efforts. It has alre...

journal_title：Cell calcium

authors： Boulay G

更新日期：2002-10-01 00:00:00

abstract：:In human embryonic kidney (HEK) cells stably transfected with green fluorescent protein targeted to the endoplasmic reticulum (ER), elevation of intracellular Ca2+ ([Ca2+]i) altered ER morphology, making it appear punctate. Electron microscopy revealed that these punctate structures represented circular and branched r...

journal_title：Cell calcium

authors： Ribeiro CM,McKay RR,Hosoki E,Bird GS,Putney JW Jr

更新日期：2000-03-01 00:00:00

abstract：:Calcium-permeable channels have traditionally been thought of as therapeutic targets in excitable cells. For instance, voltage-operated Ca2+ channels in neurones and smooth muscle cells for neurological and cardiovascular diseases although calcium-permeable channels are also functionally important in electrically non-...

journal_title：Cell calcium

authors： Li S,Westwick J,Poll C

更新日期：2003-05-01 00:00:00

abstract：:Isolated avian growth plate chondrocytes convert the acetoxymethyl ester (AM) form of Fura-2 quickly and efficiently to the Ca2(+)-sensitive pentacarboxylic acid (FA) form. Control experiments indicate that the Kd for intracellular Fura-2/FA is very close to that of extracellular Fura-2/FA at the same ionic strength a...

journal_title：Cell calcium

authors： Gunter TE,Zuscik MJ,Puzas JE,Gunter KK,Rosier RN

更新日期：1990-08-01 00:00:00

abstract：:Fluorescent calcium indicators have been widely used to assess cytoplasmic calcium concentration in cells. To examine the role of calcium ions on different physiological functions (e.g. in case of liver; bile secretion, glucose metabolism, etc.) there is a need for whole organ studies. We have developed a technique to...

journal_title：Cell calcium

authors： Ruttner Z,Ligeti L,Reinlib L,Hines K,McLaughlin AC

更新日期：1993-06-01 00:00:00

abstract：:Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...

journal_title：Cell calcium

authors： Honda Y,Miyazaki S

更新日期：1996-04-01 00:00:00

abstract：:Calcium and redox signals are presently established as essential regulators of many cellular processes. Nevertheless, we are still far from fully understanding the physiological and pathological importance of these universal second messengers. It is becoming increasingly apparent that many cellular functions are not r...

journal_title：Cell calcium

authors： Gibhardt CS,Zimmermann KM,Zhang X,Belousov VV,Bogeski I

更新日期：2016-08-01 00:00:00

abstract：:We have previously demonstrated that bradykinin potentiates prostaglandin E(2)release in human gingival fibroblasts pretreated with interleukin-1 beta (priming). In this study, we demonstrate a potentiating effect of bradykinin on cyclooxygenase-2 mRNA expression in the interleukin-1 beta-primed fibroblasts. Interleuk...

journal_title：Cell calcium

authors： Nakao S,Ogata Y,Modéer T,Segawa M,Furuyama S,Sugiya H

更新日期：2001-06-01 00:00:00

abstract：:The actions of the novel calcium (Ca2+) channel antagonist mibefradil (Ro 40-5967), a selective T-type channel blocker in myocardium, were investigated in embryonic rat spinal motoneurones maintained in culture. Whole-cell currents were recorded with the patch-clamp technique. Motoneurones displayed transient, low-vol...

journal_title：Cell calcium

authors： Viana F,Van den Bosch L,Missiaen L,Vandenberghe W,Droogmans G,Nilius B,Robberecht W

更新日期：1997-10-01 00:00:00

abstract：:Using the fluorescent Ca2+ probe Quin-2 it has been reported that cholera toxin (CT) and its B subunit (B-CT) increase cytosolic free Ca2+ concentration ([Ca2+]i) in entherocytes, thymocytes and fibroblasts. In this work we show, however, that the fluorescence increases of Quin-2-loaded cells (rat thymocytes, mouse sp...

journal_title：Cell calcium

authors： Astashkin EI,Surin AM,Mikhna MG,Nikolaeva IS,Lazarev AV,Gukovskaya AS

更新日期：1990-06-01 00:00:00

abstract：:Using single-cell ratio imaging of Fura-2-loaded neutrophils, we demonstrate that the heterogeneity and asynchrony of the oxidase response originates from variability in the timing and magnitude of the cytosolic free Ca2+ signal. The Ca2+ signals from individual cells could be classified into four types: (a) type 1, a...

journal_title：Cell calcium

authors： Hallett MB,Davies EV,Campbell AK

更新日期：1990-11-01 00:00:00

abstract：:Dynamic nuclear Ca(2+) signals play pivotal roles in diverse cellular functions including gene transcription, cell growth, differentiation, and apoptosis. Here we report a novel nuclear Ca(2+) regulatory mechanism mediated by inositol 1,4,5-trisphosphate receptors (IP(3)Rs) around the nucleus in developing cardiac myo...

journal_title：Cell calcium

authors： Luo D,Yang D,Lan X,Li K,Li X,Chen J,Zhang Y,Xiao RP,Han Q,Cheng H

更新日期：2008-02-01 00:00:00

abstract：:The effect of palmitoyl-CoA (PCoA) on Ca2+ fluxes in unfractionated SR, longitudinal tubules (LSR) and terminal cisternae (TC) subfractions, obtained from rabbit fast-twitch skeletal muscles, was investigated. After MgATP-dependent Ca2+ preloading, PCoA released Ca2+ from unfractionated SR and TC, but not from LSR. Bo...

journal_title：Cell calcium

authors： Fulceri R,Nori A,Gamberucci A,Volpe P,Giunti R,Benedetti A

更新日期：1994-02-01 00:00:00

abstract：:Aluminium lactate provokes the same modification of 45Ca(2+)-uptake by Ehrlich ascites tumour cells as does ferric lactate. The increase of uptake is metal-complex concentration-dependent, and in contrast to ferric lactate, is only partially inhibited by albumin. Under the same experimental conditions, chromic lactate...

journal_title：Cell calcium

authors： Anghileri LJ

更新日期：1992-05-01 00:00:00

abstract：:Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...

journal_title：Cell calcium

authors： Stalmans P,Himpens B

更新日期：1999-06-01 00:00:00