Abstract:
:NAADP (nicotinic acid adenine dinucleotide phosphate) is a recently discovered second messenger, and as such, we have much yet to learn about its functions in health and disease. A bottleneck in this basic research is due to NAADP, like all second messengers, being charged to prevent it from leaking out of cells. This makes for effective biology, but imposes difficulties in experiments, as it must be injected, loaded via liposomes, or electroporated, techniques that are highly technically demanding and are possible only in certain single cell preparations. For the better understood second messenger inositol 1,4,5-trisphosphate, great success has been obtained with cell-permeant derivatives where the charged groups are masked through esterification. We now report NAADP-AM as a cell-permeant analogue of NAADP that is taken up into cells and induces NAADP-mediated Ca(2+) signalling. NAADP-AM is a powerful chemical tool that will be of enormous biological utility in a wide range of systems and will greatly facilitate research into the role of NAADP in health and disease.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Parkesh R,Lewis AM,Aley PK,Arredouani A,Rossi S,Tavares R,Vasudevan SR,Rosen D,Galione A,Dowden J,Churchill GCdoi
10.1016/j.ceca.2007.08.006subject
Has Abstractpub_date
2008-06-01 00:00:00pages
531-8issue
6eissn
0143-4160issn
1532-1991pii
S0143-4160(07)00165-0journal_volume
43pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::The actions of the novel calcium (Ca2+) channel antagonist mibefradil (Ro 40-5967), a selective T-type channel blocker in myocardium, were investigated in embryonic rat spinal motoneurones maintained in culture. Whole-cell currents were recorded with the patch-clamp technique. Motoneurones displayed transient, low-vol...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90068-3
更新日期:1997-10-01 00:00:00
abstract::Calcium and calcium-dependent systems have been long implicated in the induction of epilepsy. We have previously observed that intracellular calcium ([Ca2+]i) levels remain elevated in cells undergoing epileptogenesis in the hippocampal neuronal culture (HNC) model. In this study, we employed the hippocampal neuronal ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.2000.0146
更新日期:2000-09-01 00:00:00
abstract::Voltage activated L-type Ca(2+) channels are the principal Ca(2+) channels in intestinal smooth muscle cells. They comprise the ion conducting Ca(V)1 pore and the ancillary subunits alpha(2)delta and beta. Of the four Ca(V)beta subunits Ca(V)beta(3) is assumed to be the relevant Ca(V)beta protein in smooth muscle. In ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.04.013
更新日期:2007-10-01 00:00:00
abstract::Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(86)90042-4
更新日期:1986-12-01 00:00:00
abstract::Cytosolic Ca(2+) signals are fundamental for the early and late steps of myoblast differentiation and are, as in many cells, generated by Ca(2+) release from internal stores as well as by plasma membrane Ca(2+) entry. Our recent studies identified the store-operated Ca(2+) channels, Orai1 and TRPC1&C4, as crucial for ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.10.014
更新日期:2014-12-01 00:00:00
abstract::In previous works, we have shown that L-type voltage-operated calcium channels, N-methyl-d-aspartate receptors (NMDAr), neuronal nitric oxide synthase (nNOS) and cytochrome b5 reductase (Cb5R) co-localize within the same lipid rafts-associated nanodomains in mature cerebellar granule neurons (CGN). In this work, we sh...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.06.002
更新日期:2014-08-01 00:00:00
abstract::We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% with...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90029-9
更新日期:1994-11-01 00:00:00
abstract::Corticosteroid hormones, which are released in high amounts after stress, easily pass the blood-brain-barrier. In the brain they bind to intracellular receptors which act as transcriptional regulators. These receptors are highly expressed in neurons of the hippocampal formation and the amygdala, areas that play a role...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.11.002
更新日期:2012-03-01 00:00:00
abstract::The ability of the ectodermal cells to be induced and to differentiate toward neural tissue, called neural competence, is acquired shortly before gastrulation and lost during late gastrula stages in Pleurodeles waltl embryos. We have examined ectodermal cells' neural competence in relation to the evolution of the dens...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90036-5
更新日期:1995-03-01 00:00:00
abstract::The Ca(2+) and cAMP/PKA pathways are the primary signaling systems in secretory epithelia that control virtually all secretory gland functions. Interaction and crosstalk in Ca(2+) and cAMP signaling occur at multiple levels to control and tune the activity of each other. Physiologically, Ca(2+) and cAMP signaling oper...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2014.01.006
更新日期:2014-06-01 00:00:00
abstract::Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.03.009
更新日期:2007-08-01 00:00:00
abstract::The effect of the uterotonic pituitary hormone oxytocin on the regulation of intracellular calcium concentration ([Ca2+]i) was studied in single cells of a smooth muscle cell line derived from human non-pregnant myometrium. [Ca2+]i was measured with fluorescence microscopy, and by recording the activity of Ca(2+)-acti...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90049-4
更新日期:1996-07-01 00:00:00
abstract::Calbindin D28K (formerly known as vitamin D-dependent calcium binding protein and referred to here as calbindin) is found in a wide variety of tissues, but only in certain cells within those tissues. Apart from its ability to bind calcium, nothing is known about its function in these cells. To investigate its role we ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90055-b
更新日期:1993-04-01 00:00:00
abstract::Calcium and redox signals are presently established as essential regulators of many cellular processes. Nevertheless, we are still far from fully understanding the physiological and pathological importance of these universal second messengers. It is becoming increasingly apparent that many cellular functions are not r...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2016.04.008
更新日期:2016-08-01 00:00:00
abstract::Burst firing of the thalamic neurons is driven by the low threshold Ca2+ spike generated by Ca2+ influx through T-type Ca2+ channels when these channels are activated by membrane hyperpolarization due to inhibitory inputs. The major inhibitory inputs to the thalamocortical (TC) neurons are from the GABAergic neurons i...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2006.04.023
更新日期:2006-08-01 00:00:00
abstract::Removal of the epithelium from preparations of guinea-pig airways in vitro increases the responsiveness of the smooth muscle of normal and ovalbumin-sensitized animals to a number of contractile agents. To determine if epithelium removal results in an increase in Ca2+ entry into the smooth muscle, the effect of removi...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90026-1
更新日期:1987-12-01 00:00:00
abstract::Ca2+ signalling governs stimulated exocytosis and exocytosis-coupled endocytosis also in Paramecium cells. Upon stimulation, the < or =10(3) dense-core exocytotic organelles (trichocysts) can be synchronously (80 ms) released, followed by endocytotic membrane resealing (350 ms) and retrieval. Paramecium is the most sy...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2005.06.008
更新日期:2005-09-01 00:00:00
abstract::This work shows, for the first time, a properly metabolically regulated squid nerve Na(+)/Ca(2+) exchanger (NCXSQ1) heterologous expressed in Saccharomyces cerevisiae. The exchanger was fused to the enhanced green fluorescence protein (eGFP) on its C-terminus and had two tags, a Strep-tag II and 6 histidines, added to...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.03.009
更新日期:2009-05-01 00:00:00
abstract::The non-selective second messenger-gated cation channel TRPC6 (transient receptor potential canonical 6) is activated by diacylglycerols (DAG) in a PKC-independent manner and plays important roles in a variety of physiological processes and diseases. In order to facilitate novel therapies, the development of potent in...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2018.12.009
更新日期:2019-03-01 00:00:00
abstract::Although store-operated calcium entry (SOCE) was identified more that two decades ago, understanding the molecular mechanisms that regulate and mediate this process continue to pose a major challenge to investigators in this field. Thus, there has been major focus on determining which of the models proposed for this m...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.01.013
更新日期:2007-08-01 00:00:00
abstract::Stromal interaction molecule (STIM)-1 and -2 are multi-domain, single-pass transmembrane proteins involved in sensing changes in compartmentalized calcium (Ca2+) levels and transducing this cellular signal to Orai1 channel proteins. Our understanding of the molecular mechanisms underlying STIM signaling has been drama...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2018.04.006
更新日期:2018-07-01 00:00:00
abstract::Many fluorescent Ca indicators, particularly those loaded as acetoxymethyl (AM)-ester derivatives, are known to become compartmentalized into organelles. This property can be exploited to measure changes in free [Ca] in subcellular compartments, including the inositol (1,4,5)-trisphosphate-sensitive store. However, qu...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90029-9
更新日期:1996-09-01 00:00:00
abstract::Cellular Ca2+ signals are often constrained to cytosolic micro- or nano-domains where stochastic openings of Ca2+ channels cause large fluctuations in local Ca2+ concentration (Ca2+ 'noise'). With the advent of TIRF microscopy to image the fluorescence of Ca2+-sensitive probes from attoliter volumes it has become poss...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102152
更新日期:2020-03-01 00:00:00
abstract::Cyclic AMP regulates the late step of Ca²+-dependent exocytosis in many secretory cells through two major mechanisms: a protein kinase A-dependent and a cAMP-GEF/Epac-dependent pathway. We designed a protocol to characterize the role of these two cAMP-dependent pathways on the Ca²+ sensitivity and kinetics of regulate...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.12.005
更新日期:2011-02-01 00:00:00
abstract::The effect of palmitoyl-CoA (PCoA) on Ca2+ fluxes in unfractionated SR, longitudinal tubules (LSR) and terminal cisternae (TC) subfractions, obtained from rabbit fast-twitch skeletal muscles, was investigated. After MgATP-dependent Ca2+ preloading, PCoA released Ca2+ from unfractionated SR and TC, but not from LSR. Bo...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90049-3
更新日期:1994-02-01 00:00:00
abstract::Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.11.001
更新日期:2012-03-01 00:00:00
abstract::In the presence of inositol (1,4,5)-trisphosphate (InsP3) repetitive waves of elevated cytosolic free Ca2+ (Ca waves) that travel through cellular cytoplasm are observed. Investigation of this phenomenon stimulated the view of cellular cytoplasm as 'an excitable medium composed of Ca release processes (InsP3R), couple...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90019-1
更新日期:1994-09-01 00:00:00
abstract::Intracellular Ca(2+) signals are essential for stem cell differentiation due to their ability to control signaling pathways involved in this process. Arginine vasopression (AVP) is a neurohypophyseal hormone that increases intracellular Ca(2+) concentration during adipogenesis via V1a receptors, Gq-proteins and the PL...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2015.12.006
更新日期:2016-03-01 00:00:00
abstract::Alternative splicing of the sarco/endoplasmic reticulum (SERCA2) Ca2+ pump transcript generates the two isoforms: SERCA2a in left ventricular myocytes (LVM) and SERCA2b in most tissues. Nuclear protein extracts from left ventricular myocytes can cause a decay of the 3'-region of the SERCA2a. To determine if all the do...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.06.003
更新日期:2005-01-01 00:00:00
abstract::A fluid stream induced by a concentration clamp system evokes in Xenopus oocytes a deformation of the membrane which results in transient chloride currents of high amplitude (stream-evoked inward current, I(i,st)) during calcium-activated chloride current oscillations. The involvement of cytoskeleton elements and of c...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2003.09.005
更新日期:2004-04-01 00:00:00