Abstract：
:Rabbit gall-bladder epithelial cells were isolated by a combination of Ca2+ omission, enzymatic treatment, and mechanical detachment and had a viability of 96-98% and well preserved morphology. Measurements of cytosolic free Ca2+ concentration ([Ca2+]i) in these cells with the Ca2+-fluorescent indicator fura-2 demonstrated a resting [Ca2+]i level of 115 +/- 12 nM. When used in concentrations which inhibit rabbit gall-bladder isosmotic NaCl absorption (1-100 microM), the Ca2+-channel activator BAY K 8644 caused a dose-dependent increase in the epithelial [Ca2+]i to a maximal value of 850 nM. The effect was dependent on extracellular Ca2+, and was not altered by 1 microM L-verapamil. Depolarization of the epithelial cells with KCl had no effect on [Ca2+]i. The results suggest that BAY K 8644 activates a Ca2+ influx which is not dependent on voltage-gated channels. Cytosolic Ca2+ may be involved in the regulation of isosmotic NaCl absorption in the mammalian gall-bladder.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Bouchelouche PN,Hainau B,Frederiksen Odoi
10.1016/0143-4160(89)90042-0subject
Has Abstractpub_date
1989-01-01 00:00:00pages
37-46issue
1eissn
0143-4160issn
1532-1991pii
0143-4160(89)90042-0journal_volume
10pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Neurons generate particular calcium microdomains in response to different stimuli. Calcium microdomains have a central role in a variety of neuronal functions. In particular, calcium microdomains participate in long-lasting synaptic plasticity--a neuronal response presumably correlated with cognitive brain functions t...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.021
更新日期：2006-11-01 00:00:00
abstract：:Reference treatment of advanced prostate cancer (PCa) relies on pharmacological or surgical androgen deprivation therapy. However, it is only temporarily efficient as tumor cells inevitably adapt to the low testosterone environment and become hormone-refractory (HRPCa). We observed that androgen removal in HRPCa-deriv...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.02.004
更新日期：2015-04-01 00:00:00
abstract：:Calcium and redox signals are presently established as essential regulators of many cellular processes. Nevertheless, we are still far from fully understanding the physiological and pathological importance of these universal second messengers. It is becoming increasingly apparent that many cellular functions are not r...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.04.008
更新日期：2016-08-01 00:00:00
abstract：:The fluo family of indicators is frequently used in studying Ca(2+) physiology; however, choosing which fluo indicator to use is not obvious. Indicator properties are typically determined in well-defined aqueous solutions. Inside cells, however, the properties can change markedly. We have characterized each of three f...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.05.010
更新日期：2012-08-01 00:00:00
abstract：:The concentration of free Ca2+ in the cytoplasm and organelles of individual mouse pancreatic beta-cells was estimated with dual wavelength microfluorometry and the indicators Fura-2 and furaptra. Measuring the increase of cytoplasmic Ca2+ resulting from intracellular mobilization of the ion in ob/ob mouse beta-cells,...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0087
更新日期：2000-01-01 00:00:00
abstract：:The store-operated, calcium release-activated calcium current I(CRAC) is activated by the depletion of inositol 1,4,5-trisphosphate (IP(3))-sensitive stores. The significantly different dose-response relationships of IP(3)-mediated Ca(2+) release and CRAC channel activation indicate that I(CRAC) is activated by a func...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.12.001
更新日期：2009-04-01 00:00:00
abstract：:In salivary acinar cells, muscarinic-induced fluid secretion is associated with a 1,4,5-IP3 induced increase in the cytosolic free Ca2+ concentration ([Ca2+]i), which in turn activates Ca(2+)-dependent K+ and Cl- channels that modulate the membrane potential. In the present study the influence of the membrane potentia...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90076-i
更新日期：1993-07-01 00:00:00
abstract：:A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 n...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90057-8
更新日期：1995-11-01 00:00:00
abstract：:A synthetic peptide (CaMBP) matching amino acids 3614-3643 of the skeletal ryanodine receptor (RyR1) binds to both Ca2+-free calmodulin (CaM) and Ca2+-bound CaM with nanomolar affinity [J. Biol. Chem. 276 (2001) 2069]. We report here that CaMBP increases [3H]ryanodine binding to RyR1 in a dose- and Ca2+-dependent mann...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2003.09.002
更新日期：2004-02-01 00:00:00
abstract：:The mechanisms that regulate ion and fluid transport by the human intrahepatic bile duct have not been well defined. Human intrahepatic biliary cell lines that we have developed were used to identify and characterize purinoceptors based on increases in intracellular calcium in response to ATP and other nucleotides. In...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90111-6
更新日期：1995-05-01 00:00:00
abstract：:The surface dynamics of neurotransmitter receptors and transporters, as well as ion channels, has been well-documented in neurons, revealing complex molecular behaviour and key physiological functions. However, our understanding of the membrane trafficking and dynamics of the signalling molecules located at the plasma...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.08.009
更新日期：2017-11-01 00:00:00
abstract：:Mitochondria from different organisms can undergo a sudden process of inner membrane unselective leakiness to molecules known as the mitochondrial permeability transition (MPT). This process has been studied for nearly four decades and several proteins have been claimed to constitute, or at least regulate the usually ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2018.05.003
更新日期：2018-07-01 00:00:00
abstract：:NAADP has been shown to act as a second messenger in a wide range of systems from plants to mammalian cells. Although it had always been considered as a canonical second messenger, recent work has shown that it is also active when applied extracellularly. It has also been suggested that NAADP might have a direct actio...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.10.002
更新日期：2007-06-01 00:00:00
abstract：:To characterize the effect that a phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, LY294002, has on cytosolic calcium concentrations ([Ca2+]i), bovine airway smooth muscle cells (BASMC) and cultured human bronchial smooth muscle cells (HBSMC) were loaded with fura 2-AM, imaged as single cells and [Ca2+]i measured...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(02)00111-2
更新日期：2002-07-01 00:00:00
abstract：:We present a new method for ratiometric Ca2+ measurements using indicators with excitation spectra in the visible range of wavelengths. Laser-scanning confocal microscopy was used to record intracellular Ca(2+)-signals with high temporal and spatial resolution in single cardiac myocytes. The patch-clamp technique was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90040-d
更新日期：1993-05-01 00:00:00
abstract：:Three decades ago, James W. Putney Jr. conceptualized the idea of store-operated calcium entry (SOCE) to explain how depletion of endoplasmic reticulum (ER) Ca2+ stores evokes Ca2+ influx across the plasma membrane. Since the publication of this highly influential idea, it is now established that SOCE is universal amo...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.12.006
更新日期：2017-05-01 00:00:00
abstract：:Using single-cell ratio imaging of Fura-2-loaded neutrophils, we demonstrate that the heterogeneity and asynchrony of the oxidase response originates from variability in the timing and magnitude of the cytosolic free Ca2+ signal. The Ca2+ signals from individual cells could be classified into four types: (a) type 1, a...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90020-u
更新日期：1990-11-01 00:00:00
abstract：:P2Y(12) antagonists such as clopidogrel and AR-C69931MX inhibit aggregation by antagonizing the effects of ADP at P2Y(12) receptors on platelets. Agents such as PGE(1) also inhibit aggregation by stimulating adenylate cyclase to produce cAMP, which interferes with Ca(2+) mobilization within the cell. Since one facet o...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00170-2
更新日期：2004-01-01 00:00:00
abstract：:Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.08.003
更新日期：2011-11-01 00:00:00
abstract：:Antigen-mediated mast cell (MC) degranulation is the critical early event in the induction of allergic reactions. Transient receptor potential channels (TRPC), particularly TRPC1, are thought to contribute to such MC activation. To explore the contribution of TRPC1 in MC-driven allergic reactions, we examined antigen-...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.02.001
更新日期：2013-05-01 00:00:00
abstract：:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel playing a major role in Ca2+ signaling. Three isoforms of IP3R have been identified and most cell types express different proportions of each isoform. The DT40 B lymphocyte cell line lacking all three IP3R isoform...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2004.03.005
更新日期：2005-02-01 00:00:00
abstract：:Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.11.001
更新日期：2012-03-01 00:00:00
abstract：:The non-selective second messenger-gated cation channel TRPC6 (transient receptor potential canonical 6) is activated by diacylglycerols (DAG) in a PKC-independent manner and plays important roles in a variety of physiological processes and diseases. In order to facilitate novel therapies, the development of potent in...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.12.009
更新日期：2019-03-01 00:00:00
abstract：:Indo-1 is a new fluorescent indicator of the intracellular free calcium concentration Cai++. Indo-1 may be used in a similar manner as its predecessor quin2 but offers the principal advantage that the Ca++ saturated form of the Ca++ chelator has a emission maximum different in wavelength from that of free indo-1 (400 ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(86)90003-5
更新日期：1986-08-01 00:00:00
abstract：:The importance of Ca2+-signaling for many subcellular processes is well established in higher eukaryotes, whereas information about protozoa is restricted. Recent genome analyses have stimulated such work also with Alveolates, such as ciliates (Paramecium, Tetrahymena) and their pathogenic close relatives, the Apicomp...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.01.006
更新日期：2012-05-01 00:00:00
abstract：:The effect of furosemide and ouabain on the intracellular concentration of Ca2+ was studies in myocardial cell cultures using Fura-2, a fluorescent agent, as an intracellular Ca2+ indicator. Introduction of 200 microM ouabain to the cultured cells increased the intracellular calcium concentration from an average of 23...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90004-7
更新日期：1995-08-01 00:00:00
abstract：:Ca2+ signalling governs stimulated exocytosis and exocytosis-coupled endocytosis also in Paramecium cells. Upon stimulation, the < or =10(3) dense-core exocytotic organelles (trichocysts) can be synchronously (80 ms) released, followed by endocytotic membrane resealing (350 ms) and retrieval. Paramecium is the most sy...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2005.06.008
更新日期：2005-09-01 00:00:00
abstract：:Changes in the concentration and spatial distribution of Ca(2+) ions in the cytoplasm constitute a ubiquitous intracellular signaling module in cellular physiology. With the advent of Ca(2+) dyes that allow direct visualization of Ca(2+) transients, combined with powerful experimental tools such as electrophysiologica...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2010.10.003
更新日期：2010-11-01 00:00:00
abstract：:Mechanically induced channel activities and increase of intracellular calcium ([Ca2+]i) in normal and cancerous murine mammary cells (MMT 060562) were investigated using the patch clamp technique and Fura-2 fluorescence. Both cell types showed similar properties. Upon mechanical stimulation, activation of the Ca(2+)-d...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90018-n
更新日期：1992-08-01 00:00:00
abstract：:Receptors for extracellular nucleotides (the P2X-calcium channels and the phospholipase C-coupled P2Y receptors) play key roles in pain signaling, but little is known on their function in trigeminal ganglia, whose hyperactivation leads to the development of migraine pain. Here we characterize calcium signaling via P2X...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.10.003
更新日期：2008-06-01 00:00:00
