Intracellular concentrations of fura-2 and fura-2/am in vascular smooth muscle cells following perfusion loading of fura-2/am in arterial segments.

abstract：:The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) mediates increases in the intracellular concentration of Ca(2+) ([Ca(2+)]i) during fertilization in mammalian eggs. The activity of IP3R1 is enhanced during oocyte maturation, and phosphorylations by M-phase kinases are thought to positively regulate the activit...

journal_title：Cell calcium

authors： Zhang N,Yoon SY,Parys JB,Fissore RA

更新日期：2015-11-01 00:00:00

abstract：:Although store-operated calcium entry (SOCE) was identified more that two decades ago, understanding the molecular mechanisms that regulate and mediate this process continue to pose a major challenge to investigators in this field. Thus, there has been major focus on determining which of the models proposed for this m...

journal_title：Cell calcium

authors： Ambudkar IS,Ong HL,Liu X,Bandyopadhyay BC,Cheng KT

更新日期：2007-08-01 00:00:00

abstract：:Calcium-permeable channels have traditionally been thought of as therapeutic targets in excitable cells. For instance, voltage-operated Ca2+ channels in neurones and smooth muscle cells for neurological and cardiovascular diseases although calcium-permeable channels are also functionally important in electrically non-...

journal_title：Cell calcium

authors： Li S,Westwick J,Poll C

更新日期：2003-05-01 00:00:00

abstract：:We have recently shown that S-100b protein interacts with the polar surface of cardiolipin vesicles [6]. This interaction produces changes in the secondary structure of S-100b as well as changes in the structural organization of cardiolipin vesicles. We report here on the effects of S-100b on cardiolipin vesicles as i...

journal_title：Cell calcium

authors： Zolese G,Curatola G,Amati S,Giambanco I,Donato R

更新日期：1990-01-01 00:00:00

abstract：:We describe experiments in which the low affinity indicator Oregon Green BAPTA 5N was used to record the spatially resolved changes in [Ca(2+)] from intracellular stores in rat gastric myocytes. Cells were loaded with the membrane permeant form of the indicator and imaged using a confocal scanning laser microscope. In...

journal_title：Cell calcium

authors： White C,McGeown G

更新日期：2002-04-01 00:00:00

abstract：:Amyloid beta (Aβ) oligomers accumulate in the brain tissue of Alzheimer disease patients and are related to disease pathogenesis. The precise mechanisms by which Aβ oligomers cause neurotoxicity remain unknown. We recently reported that Aβ oligomers cause intracellular Ca(2+) overload and neuronal death that can be pr...

journal_title：Cell calcium

authors： Texidó L,Martín-Satué M,Alberdi E,Solsona C,Matute C

更新日期：2011-03-01 00:00:00

abstract：:The highly differentiated structure of the neuron poses special problems for the intracellular movement of molecules throughout the cell. Molecular transport distances from the synthesizing neuron cell body along the axon (which has no substantial synthetic capabilities) to the axon terminal are very great. The transp...

journal_title：Cell calcium

authors： Breuer AC,Atkinson MB

更新日期：1988-12-01 00:00:00

abstract：:The formation of a heterotetrameric complex between annexin 2 (anx 2) and S100A10 plays an important role in regulating the cellular distribution and biochemical properties of anx 2. A major distinction between the anx 2-S100A10 complex and other annexin-S100 complexes is that S100A10 binding to anx 2 occurs independe...

journal_title：Cell calcium

authors： Borthwick LA,Neal A,Hobson L,Gerke V,Robson L,Muimo R

更新日期：2008-08-01 00:00:00

abstract：:Atypical absence epilepsy (AAE) is an intractable disorder characterized by slow spike-and-wave discharges in electroencephalograms (EEGs) and accompanied by severe cognitive dysfunction and neurodevelopmental or neurological deficits in humans. Administration of the cholesterol biosynthesis inhibitor AY-9944 (AY) dur...

journal_title：Cell calcium

authors： Jung S,Jeong Y,Jeon D

更新日期：2015-05-01 00:00:00

abstract：:Under certain conditions of Ca2+ loading, cardiac myocytes, both isolated and in intact tissue, exhibit spontaneous, oscillatory Ca2+ transients due to Ca2+ release from the sarcoplasmic reticulum. These transients are not triggered by depolarization of the sarcolemma, though they themselves can generate depolarizing ...

journal_title：Cell calcium

authors： Stern MD,Capogrossi MC,Lakatta EG

更新日期：1988-12-01 00:00:00

abstract：:Calcium dependence of bleb formation and cell death was evaluated in rat hepatocytes following ATP depletion by metabolic inhibition with KCN and iodoacetate ('chemical hypoxia'). Cytosolic free Ca2+ was measured in single cells by ratio imaging of Fura-2 fluorescence using multiparameter digitized video microscopy. C...

journal_title：Cell calcium

authors： Nieminen AL,Gores GJ,Wray BE,Tanaka Y,Herman B,Lemasters JJ

更新日期：1988-12-01 00:00:00

abstract：:It has been reported that leukotriene B4 can translocate calcium across model membranes (Serhan et. al., (1982) J. Biol. Chem., 257: 4746). Such ionophoretic behavior could account for its biological effects. We have examined the effect of chromatographically pure leukotriene B4 on Ca2+ permeability when added exogeno...

journal_title：Cell calcium

authors： Holmes RP,Yoss NL,Marshall LA

更新日期：1987-12-01 00:00:00

abstract：:Parvalbumin (PA) is a Ca(2+)-binding protein of vertebrates massively expressed in tissues with high oxygen uptake and respectively elevated level of reactive oxygen species (ROS). To characterize antioxidant properties of PA, antioxidant capacity (AOC) of intact rat α-PA has been explored. ORAC, TEAC and hydrogen per...

journal_title：Cell calcium

authors： Permyakov SE,Kazakov AS,Avkhacheva NV,Permyakov EA

更新日期：2014-05-01 00:00:00

abstract：:Specific Ca(2+) homeostatic system appeared very early in the history of the cell, as a survival system preventing Ca(2+)-mediated cell damage. This homeostatic system produced a steep ( approximately 20,000 times) concentration gradient between extracellular and intracellular compartments, which has both survival imp...

journal_title：Cell calcium

authors： Case RM,Eisner D,Gurney A,Jones O,Muallem S,Verkhratsky A

更新日期：2007-10-01 00:00:00

abstract：:The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...

journal_title：Cell calcium

authors： Tiffert T,Lew VL

更新日期：2001-11-01 00:00:00

abstract：:In murine ventricular myocytes, activation of mechanosensitive ion channels (MSCs) includes activation of non-selective cation currents and deactivation of inwardly rectifying potassium currents. Using pharmacological inhibitors and knockout models, we analyzed signaling steps that are critical to transduce the mechan...

journal_title：Cell calcium

authors： Dyachenko V,Rueckschloss U,Isenberg G

更新日期：2009-01-01 00:00:00

abstract：:Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...

journal_title：Cell calcium

authors： Chang JP,Habibi HR,Yu Y,Moussavi M,Grey CL,Pemberton JG

更新日期：2012-03-01 00:00:00

abstract：:Alternative splicing of the sarco/endoplasmic reticulum (SERCA2) Ca2+ pump transcript generates the two isoforms: SERCA2a in left ventricular myocytes (LVM) and SERCA2b in most tissues. Nuclear protein extracts from left ventricular myocytes can cause a decay of the 3'-region of the SERCA2a. To determine if all the do...

journal_title：Cell calcium

authors： Misquitta CM,Ghosh P,Mwanjewe J,Grover AK

更新日期：2005-01-01 00:00:00

abstract：:Quartered pituitaries from castrated (72 h) +/- estradiol (E2)-treated (24 h) rats were used in a perifusion system to investigate estradiol modulation of ionomycin and ionomycin + PMA stimulated LH secretion. Estradiol enhanced the LH responses to GnRH (1 nM) and ionomycin (10 microM), and was necessary for the manif...

journal_title：Cell calcium

authors： Fahmy NW,Bourne GA

更新日期：1993-01-01 00:00:00

abstract：:The best known calcium waves move at about 5-30 microns/s (at 20 degrees C) and will be called fast waves to distinguish them from slow (contractile) ones which move at 0.1-1 microns/s as well as electrically propagated, ultrafast ones. Fast waves move deep within cells and seem to underlie most calcium signals. Their...

journal_title：Cell calcium

authors： Jaffe LF

更新日期：1993-11-01 00:00:00

abstract：:For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed he...

journal_title：Cell calcium

authors： Grover AK

更新日期：1985-06-01 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a progressive muscle disease with severe cardiac complications. It is believed that cellular oxidative stress and augmented Ca(2+) signaling drives the development of cardiac pathology. Some mitochondrial and metabolic dysfunctions have also been reported. Here we investigate cellu...

journal_title：Cell calcium

authors： Kyrychenko V,Poláková E,Janíček R,Shirokova N

更新日期：2015-08-01 00:00:00

abstract：:Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...

journal_title：Cell calcium

authors： Hewavitharana T,Deng X,Soboloff J,Gill DL

更新日期：2007-08-01 00:00:00

abstract：:Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...

journal_title：Cell calcium

authors： Medic N,Desai A,Komarow H,Burch LH,Bandara G,Beaven MA,Metcalfe DD,Gilfillan AM

更新日期：2011-11-01 00:00:00

abstract：:The importance of Ca2+-signaling for many subcellular processes is well established in higher eukaryotes, whereas information about protozoa is restricted. Recent genome analyses have stimulated such work also with Alveolates, such as ciliates (Paramecium, Tetrahymena) and their pathogenic close relatives, the Apicomp...

journal_title：Cell calcium

authors： Plattner H,Sehring IM,Mohamed IK,Miranda K,De Souza W,Billington R,Genazzani A,Ladenburger EM

更新日期：2012-05-01 00:00:00

abstract：:We investigated whether the expression of human alpha-parvalbumin affects depolarization-induced elevations of the cytosolic free calcium concentration ([Ca2+]i) in human neuroblastoma SKNBE2 cells. A full length human parvalbumin cDNA was cloned by PCR from human cerebellum and transiently transfected into SKNBE2 cel...

journal_title：Cell calcium

authors： Dreessen J,Lutum C,Schäfer BW,Heizmann CW,Knöpfel T

更新日期：1996-06-01 00:00:00

abstract：:Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...

journal_title：Cell calcium

authors： Fliegert R,Hölzer HT,Guse AH

更新日期：2018-12-01 00:00:00

abstract：:Ryanodine receptors plays a crucial role in skeletal muscle excitation-contraction coupling by releasing calcium ions required for muscle contraction from the sarcoplasmic reticulum. At least three phenotypes associated with more than 100 RYR1 mutations have been identified; in order to elucidate possible pathophysiol...

journal_title：Cell calcium

authors： Ghassemi F,Vukcevic M,Xu L,Zhou H,Meissner G,Muntoni F,Jungbluth H,Zorzato F,Treves S

更新日期：2009-02-01 00:00:00

abstract：:The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...

journal_title：Cell calcium

authors： De Marchi E,Bonora M,Giorgi C,Pinton P

更新日期：2014-07-01 00:00:00

abstract：:Using the low-affinity fluorescent Ca(2+) indicators, Mag-Fura-2 and Mag-Fura Red, we studied light- and InsP(3)-induced Ca(2+) release in permeabilized microvillar photoreceptors of the medicinal leech, Hirudo medicinalis. Two major components of the phosphoinositide signaling pathway, phospholipase-C and the InsP(3)...

journal_title：Cell calcium

authors： Ukhanov K,Mills SJ,Potter BV,Walz B

更新日期：2001-05-01 00:00:00