Intracellular concentrations of fura-2 and fura-2/am in vascular smooth muscle cells following perfusion loading of fura-2/am in arterial segments.


:A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 nM for Fura-2 and Fura-2/AM, respectively. When this technique was applied to perfusion-loaded segments of the rat tail artery, intracellular concentrations of Fura-2 determined by tissue disruption were 10 times those obtained by comparing the increase in fluorescence at the isoemissive point (following loading), with a calibration curve for Fura-2. Loading conditions of 90 min at [Fura-2/AM]e = 5 microM were optimal in terms of [Fura-2]i which attained a concentration not significantly different from [Fura-2/AM]e. Under such conditions, however, Fura-2/AM also accumulated in the arterial wall. Although incompletely de-esterified, Fura-2/AM metabolites produced by in vitro incubation of Fura-2/AM with pig liver esterases could be easily detected, fluorescent forms of Fura-2 with a different sensitivity for calcium were not detected in arterial extracts.


Cell Calcium


Cell calcium


Tran NN,Leroy P,Bellucci L,Robert A,Nicolas A,Atkinson J,Capdeville-Atkinson C




Has Abstract


1995-11-01 00:00:00














  • Effect of M-phase kinase phosphorylations on type 1 inositol 1,4,5-trisphosphate receptor-mediated Ca2+ responses in mouse eggs.

    abstract::The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) mediates increases in the intracellular concentration of Ca(2+) ([Ca(2+)]i) during fertilization in mammalian eggs. The activity of IP3R1 is enhanced during oocyte maturation, and phosphorylations by M-phase kinases are thought to positively regulate the activit...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Zhang N,Yoon SY,Parys JB,Fissore RA

    更新日期:2015-11-01 00:00:00

  • TRPC1: the link between functionally distinct store-operated calcium channels.

    abstract::Although store-operated calcium entry (SOCE) was identified more that two decades ago, understanding the molecular mechanisms that regulate and mediate this process continue to pose a major challenge to investigators in this field. Thus, there has been major focus on determining which of the models proposed for this m...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Ambudkar IS,Ong HL,Liu X,Bandyopadhyay BC,Cheng KT

    更新日期:2007-08-01 00:00:00

  • Transient receptor potential (TRP) channels as potential drug targets in respiratory disease.

    abstract::Calcium-permeable channels have traditionally been thought of as therapeutic targets in excitable cells. For instance, voltage-operated Ca2+ channels in neurones and smooth muscle cells for neurological and cardiovascular diseases although calcium-permeable channels are also functionally important in electrically non-...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Li S,Westwick J,Poll C

    更新日期:2003-05-01 00:00:00

  • S-100b protein regulates aggregation and fusion of cardiolipin vesicles.

    abstract::We have recently shown that S-100b protein interacts with the polar surface of cardiolipin vesicles [6]. This interaction produces changes in the secondary structure of S-100b as well as changes in the structural organization of cardiolipin vesicles. We report here on the effects of S-100b on cardiolipin vesicles as i...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Zolese G,Curatola G,Amati S,Giambanco I,Donato R

    更新日期:1990-01-01 00:00:00

  • Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy.

    abstract::We describe experiments in which the low affinity indicator Oregon Green BAPTA 5N was used to record the spatially resolved changes in [Ca(2+)] from intracellular stores in rat gastric myocytes. Cells were loaded with the membrane permeant form of the indicator and imaged using a confocal scanning laser microscope. In...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: White C,McGeown G

    更新日期:2002-04-01 00:00:00

  • Amyloid β peptide oligomers directly activate NMDA receptors.

    abstract::Amyloid beta (Aβ) oligomers accumulate in the brain tissue of Alzheimer disease patients and are related to disease pathogenesis. The precise mechanisms by which Aβ oligomers cause neurotoxicity remain unknown. We recently reported that Aβ oligomers cause intracellular Ca(2+) overload and neuronal death that can be pr...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Texidó L,Martín-Satué M,Alberdi E,Solsona C,Matute C

    更新日期:2011-03-01 00:00:00

  • Calcium dependent modulation of fast axonal transport.

    abstract::The highly differentiated structure of the neuron poses special problems for the intracellular movement of molecules throughout the cell. Molecular transport distances from the synthesizing neuron cell body along the axon (which has no substantial synthetic capabilities) to the axon terminal are very great. The transp...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Breuer AC,Atkinson MB

    更新日期:1988-12-01 00:00:00

  • The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia.

    abstract::The formation of a heterotetrameric complex between annexin 2 (anx 2) and S100A10 plays an important role in regulating the cellular distribution and biochemical properties of anx 2. A major distinction between the anx 2-S100A10 complex and other annexin-S100 complexes is that S100A10 binding to anx 2 occurs independe...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Borthwick LA,Neal A,Hobson L,Gerke V,Robson L,Muimo R

    更新日期:2008-08-01 00:00:00

  • Epileptic activity during early postnatal life in the AY-9944 model of atypical absence epilepsy.

    abstract::Atypical absence epilepsy (AAE) is an intractable disorder characterized by slow spike-and-wave discharges in electroencephalograms (EEGs) and accompanied by severe cognitive dysfunction and neurodevelopmental or neurological deficits in humans. Administration of the cholesterol biosynthesis inhibitor AY-9944 (AY) dur...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Jung S,Jeong Y,Jeon D

    更新日期:2015-05-01 00:00:00

  • Spontaneous calcium release from the sarcoplasmic reticulum in myocardial cells: mechanisms and consequences.

    abstract::Under certain conditions of Ca2+ loading, cardiac myocytes, both isolated and in intact tissue, exhibit spontaneous, oscillatory Ca2+ transients due to Ca2+ release from the sarcoplasmic reticulum. These transients are not triggered by depolarization of the sarcolemma, though they themselves can generate depolarizing ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Stern MD,Capogrossi MC,Lakatta EG

    更新日期:1988-12-01 00:00:00

  • Calcium dependence of bleb formation and cell death in hepatocytes.

    abstract::Calcium dependence of bleb formation and cell death was evaluated in rat hepatocytes following ATP depletion by metabolic inhibition with KCN and iodoacetate ('chemical hypoxia'). Cytosolic free Ca2+ was measured in single cells by ratio imaging of Fura-2 fluorescence using multiparameter digitized video microscopy. C...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Nieminen AL,Gores GJ,Wray BE,Tanaka Y,Herman B,Lemasters JJ

    更新日期:1988-12-01 00:00:00

  • Failure of leukotriene B4 to translocate calcium across phosphatidylcholine membranes.

    abstract::It has been reported that leukotriene B4 can translocate calcium across model membranes (Serhan et. al., (1982) J. Biol. Chem., 257: 4746). Such ionophoretic behavior could account for its biological effects. We have examined the effect of chromatographically pure leukotriene B4 on Ca2+ permeability when added exogeno...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Holmes RP,Yoss NL,Marshall LA

    更新日期:1987-12-01 00:00:00

  • Parvalbumin as a metal-dependent antioxidant.

    abstract::Parvalbumin (PA) is a Ca(2+)-binding protein of vertebrates massively expressed in tissues with high oxygen uptake and respectively elevated level of reactive oxygen species (ROS). To characterize antioxidant properties of PA, antioxidant capacity (AOC) of intact rat α-PA has been explored. ORAC, TEAC and hydrogen per...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Permyakov SE,Kazakov AS,Avkhacheva NV,Permyakov EA

    更新日期:2014-05-01 00:00:00

  • Evolution of calcium homeostasis: from birth of the first cell to an omnipresent signalling system.

    abstract::Specific Ca(2+) homeostatic system appeared very early in the history of the cell, as a survival system preventing Ca(2+)-mediated cell damage. This homeostatic system produced a steep ( approximately 20,000 times) concentration gradient between extracellular and intracellular compartments, which has both survival imp...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Case RM,Eisner D,Gurney A,Jones O,Muallem S,Verkhratsky A

    更新日期:2007-10-01 00:00:00

  • Kinetics of inhibition of the plasma membrane calcium pump by vanadate in intact human red cells.

    abstract::The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Tiffert T,Lew VL

    更新日期:2001-11-01 00:00:00

  • Modulation of cardiac mechanosensitive ion channels involves superoxide, nitric oxide and peroxynitrite.

    abstract::In murine ventricular myocytes, activation of mechanosensitive ion channels (MSCs) includes activation of non-selective cation currents and deactivation of inwardly rectifying potassium currents. Using pharmacological inhibitors and knockout models, we analyzed signaling steps that are critical to transduce the mechan...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Dyachenko V,Rueckschloss U,Isenberg G

    更新日期:2009-01-01 00:00:00

  • Calcium and other signalling pathways in neuroendocrine regulation of somatotroph functions.

    abstract::Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Chang JP,Habibi HR,Yu Y,Moussavi M,Grey CL,Pemberton JG

    更新日期:2012-03-01 00:00:00

  • Control of SERCA2a Ca2+ pump mRNA stability by nuclear proteins: role of domains in the 3'-untranslated region.

    abstract::Alternative splicing of the sarco/endoplasmic reticulum (SERCA2) Ca2+ pump transcript generates the two isoforms: SERCA2a in left ventricular myocytes (LVM) and SERCA2b in most tissues. Nuclear protein extracts from left ventricular myocytes can cause a decay of the 3'-region of the SERCA2a. To determine if all the do...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Misquitta CM,Ghosh P,Mwanjewe J,Grover AK

    更新日期:2005-01-01 00:00:00

  • Estradiol modulation of PMA--and ionomycin-stimulated LH secretion from pituitaries of castrated rats.

    abstract::Quartered pituitaries from castrated (72 h) +/- estradiol (E2)-treated (24 h) rats were used in a perifusion system to investigate estradiol modulation of ionomycin and ionomycin + PMA stimulated LH secretion. Estradiol enhanced the LH responses to GnRH (1 nM) and ionomycin (10 microM), and was necessary for the manif...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Fahmy NW,Bourne GA

    更新日期:1993-01-01 00:00:00

  • Classes and mechanisms of calcium waves.

    abstract::The best known calcium waves move at about 5-30 microns/s (at 20 degrees C) and will be called fast waves to distinguish them from slow (contractile) ones which move at 0.1-1 microns/s as well as electrically propagated, ultrafast ones. Fast waves move deep within cells and seem to underlie most calcium signals. Their...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Jaffe LF

    更新日期:1993-11-01 00:00:00

  • Ca-pumps in smooth muscle: one in plasma membrane and another in endoplasmic reticulum.

    abstract::For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed he...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Grover AK

    更新日期:1985-06-01 00:00:00

  • Mitochondrial dysfunctions during progression of dystrophic cardiomyopathy.

    abstract::Duchenne muscular dystrophy (DMD) is a progressive muscle disease with severe cardiac complications. It is believed that cellular oxidative stress and augmented Ca(2+) signaling drives the development of cardiac pathology. Some mitochondrial and metabolic dysfunctions have also been reported. Here we investigate cellu...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Kyrychenko V,Poláková E,Janíček R,Shirokova N

    更新日期:2015-08-01 00:00:00

  • Role of STIM and Orai proteins in the store-operated calcium signaling pathway.

    abstract::Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Hewavitharana T,Deng X,Soboloff J,Gill DL

    更新日期:2007-08-01 00:00:00

  • Examination of the role of TRPM8 in human mast cell activation and its relevance to the etiology of cold-induced urticaria.

    abstract::Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Medic N,Desai A,Komarow H,Burch LH,Bandara G,Beaven MA,Metcalfe DD,Gilfillan AM

    更新日期:2011-11-01 00:00:00

  • Calcium signaling in closely related protozoan groups (Alveolata): non-parasitic ciliates (Paramecium, Tetrahymena) vs. parasitic Apicomplexa (Plasmodium, Toxoplasma).

    abstract::The importance of Ca2+-signaling for many subcellular processes is well established in higher eukaryotes, whereas information about protozoa is restricted. Recent genome analyses have stimulated such work also with Alveolates, such as ciliates (Paramecium, Tetrahymena) and their pathogenic close relatives, the Apicomp...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Plattner H,Sehring IM,Mohamed IK,Miranda K,De Souza W,Billington R,Genazzani A,Ladenburger EM

    更新日期:2012-05-01 00:00:00

  • Alpha-parvalbumin reduces depolarization-induced elevations of cytosolic free calcium in human neuroblastoma cells.

    abstract::We investigated whether the expression of human alpha-parvalbumin affects depolarization-induced elevations of the cytosolic free calcium concentration ([Ca2+]i) in human neuroblastoma SKNBE2 cells. A full length human parvalbumin cDNA was cloned by PCR from human cerebellum and transiently transfected into SKNBE2 cel...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Dreessen J,Lutum C,Schäfer BW,Heizmann CW,Knöpfel T

    更新日期:1996-06-01 00:00:00

  • TRPM2 activation: Paradigm shifted?

    abstract::Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...

    journal_title:Cell calcium

    pub_type: 评论,杂志文章


    authors: Fliegert R,Hölzer HT,Guse AH

    更新日期:2018-12-01 00:00:00

  • A recessive ryanodine receptor 1 mutation in a CCD patient increases channel activity.

    abstract::Ryanodine receptors plays a crucial role in skeletal muscle excitation-contraction coupling by releasing calcium ions required for muscle contraction from the sarcoplasmic reticulum. At least three phenotypes associated with more than 100 RYR1 mutations have been identified; in order to elucidate possible pathophysiol...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Ghassemi F,Vukcevic M,Xu L,Zhou H,Meissner G,Muntoni F,Jungbluth H,Zorzato F,Treves S

    更新日期:2009-02-01 00:00:00

  • The mitochondrial permeability transition pore is a dispensable element for mitochondrial calcium efflux.

    abstract::The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: De Marchi E,Bonora M,Giorgi C,Pinton P

    更新日期:2014-07-01 00:00:00

  • InsP(3)-induced Ca(2+) release in permeabilized invertebrate photoreceptors: a link between phototransduction and Ca(2+) stores.

    abstract::Using the low-affinity fluorescent Ca(2+) indicators, Mag-Fura-2 and Mag-Fura Red, we studied light- and InsP(3)-induced Ca(2+) release in permeabilized microvillar photoreceptors of the medicinal leech, Hirudo medicinalis. Two major components of the phosphoinositide signaling pathway, phospholipase-C and the InsP(3)...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Ukhanov K,Mills SJ,Potter BV,Walz B

    更新日期:2001-05-01 00:00:00