Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy.


:We describe experiments in which the low affinity indicator Oregon Green BAPTA 5N was used to record the spatially resolved changes in [Ca(2+)] from intracellular stores in rat gastric myocytes. Cells were loaded with the membrane permeant form of the indicator and imaged using a confocal scanning laser microscope. In doubly stained cells the Oregon Green signal colocalized with BIODIPY 558/568 Brefeldin A, a label for the endo/sarcoplasmic reticulum (SR) and Golgi apparatus. Oregon Green BAPTA 5N was calibrated in gastric myocytes, giving an in situ K(d) of 90 microM. The resting free [Ca(2+)] within the SR averaged 65 microM. A reversible decrease in Oregon Green fluorescence was observed on bath application of Inositol triphosphate (IP(3)) (10 microM) to permeabilized cells. Similar changes were also observed when cyclopiazonic acid (5 microM) was applied to intact myocytes, again with recovery of store [Ca(2+)] following drug washout. Identical patterns of Ca(2+) depletion were seen when caffeine (1 microM) and carbachol (10 microM) were applied sequentially to the same cells, suggesting that activation of ryanodine and IP(3)-sensitive channels can result in the release of Ca(2+) from the same regions of the SR.


Cell Calcium


Cell calcium


White C,McGeown G





Has Abstract


2002-04-01 00:00:00














  • LY294002, but not wortmannin, increases intracellular calcium and inhibits calcium transients in bovine and human airway smooth muscle cells.

    abstract::To characterize the effect that a phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, LY294002, has on cytosolic calcium concentrations ([Ca2+]i), bovine airway smooth muscle cells (BASMC) and cultured human bronchial smooth muscle cells (HBSMC) were loaded with fura 2-AM, imaged as single cells and [Ca2+]i measured...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Ethier MF,Madison JM

    更新日期:2002-07-01 00:00:00

  • Protein kinase C decreases the apparent affinity of the inositol 1,4,5-trisphosphate receptor type 3 in RINm5F cells.

    abstract::In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel which plays a major role in Ca2+ signalling. Three isoforms of IP3R have been identified (IP3R-1, IP3R-2 and IP3R-3) and most cell types express different proportions of each isoform. The differences between the p...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Caron AZ,Chaloux B,Arguin G,Guillemette G

    更新日期:2007-09-01 00:00:00

  • Activity-independent intracellular Ca2+ oscillations are spontaneously generated by ventral spinal neurons during development in vitro.

    abstract::Within the CNS, distinct neurons may rely on different processes to modulate cytosolic Ca2+ depending on the network developmental phase. In particular, in the immature spinal cord, synchronous electrical discharges are coupled with biochemical signals triggered by intracellular Ca2+ waves. Nevertheless, the presence ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Fabbro A,Pastore B,Nistri A,Ballerini L

    更新日期:2007-04-01 00:00:00

  • Temperature and nitric oxide control spontaneous calcium transients in astrocytes.

    abstract::Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo e...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Schipke CG,Heidemann A,Skupin A,Peters O,Falcke M,Kettenmann H

    更新日期:2008-03-01 00:00:00

  • Apparent stability constants and purity of Ca-chelating agents evaluated using Ca-selective electrodes by the double-log optimization method.

    abstract::Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Oiki S,Yamamoto T,Okada Y

    更新日期:1994-03-01 00:00:00

  • Contribution of capacitative and non-capacitative Ca2+-entry to M3-receptor-mediated contraction of porcine coronary smooth muscle.

    abstract::We studied the contribution of store-operated or capacitative Ca2+-entry (SOCE or CCE, respectively) through store-operated Ca2+ channels (SOCCs) and the contribution of Ca2+-entry through receptor-operated, non-selective cation channels (ROCCs or NSCCs, respectively), on the M3-receptor-mediated (270 nM Ach) contract...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Weirich J,Dumont L,Fleckenstein-Grün G

    更新日期:2005-11-01 00:00:00

  • Evolution of the Ca2+ current during dialysis of isolated bovine chromaffin cells: effect of internal calcium.

    abstract::We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% with...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Elhamdani A,Bossu JL,Feltz A

    更新日期:1994-11-01 00:00:00

  • The role of the calcium-sensing receptor in epidermal differentiation.

    abstract::Calcium regulates the proliferation and differentiation of keratinocytes both in vivo and in vitro. Elevated extracellular Ca2+ concentration ([Ca2+]o) raises the intracellular free calcium ([Ca2+]i) and activates differentiation-related genes. Cells lacking the calcium-sensing receptor (CaR) fail to respond to [Ca2+]...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Tu CL,Oda Y,Komuves L,Bikle DD

    更新日期:2004-03-01 00:00:00

  • ET-1-induced growth promoting responses involving ERK1/2 and PKB signaling and Egr-1 expression are mediated by Ca2+/CaM-dependent protein kinase-II in vascular smooth muscle cells.

    abstract::Endothelin-1 (ET-1), a potent vasoactive peptide with a pathogenic role in vascular diseases, has been shown to induce the activation of ERK1/2, PKB and the expression of a transcriptional regulator, the early growth response 1 (Egr-1), key mediators of hypertrophic and proliferative responses in vascular smooth muscl...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Bouallegue A,Simo Cheyou ER,Anand-Srivastava MB,Srivastava AK

    更新日期:2013-12-01 00:00:00

  • Mitochondrial and endoplasmic reticulum calcium homeostasis and cell death.

    abstract::The endoplasmic reticulum (ER) and mitochondria cannot be considered as static structures, as they intimately communicate, forming very dynamic platforms termed mitochondria-associated membranes (MAMs). In particular, the ER transmits proper Ca2+ signals to mitochondria, which decode them into specific inputs to regul...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Marchi S,Patergnani S,Missiroli S,Morciano G,Rimessi A,Wieckowski MR,Giorgi C,Pinton P

    更新日期:2018-01-01 00:00:00

  • TRPV4 channels activity in bovine articular chondrocytes: regulation by obesity-associated mediators.

    abstract::Turnover of the cartilage extracellular matrix depends exclusively on chondrocytes and varies in response to load and osmolarity fluctuations. Obesity can affect chondrocyte physiology; adipokines, insulin and proinflammatory cytokines levels are all altered in the obese and are related to matrix turnover impairments ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Sánchez JC,López-Zapata DF,Wilkins RJ

    更新日期:2014-12-01 00:00:00

  • Bcl-2 interaction with the inositol 1,4,5-trisphosphate receptor: role in Ca(2+) signaling and disease.

    abstract::The Bcl-2 protein, best known for its ability to inhibit apoptosis, interacts with the inositol 1,4,5-trisphosphate receptor (IP(3)R) Ca(2+) channel to regulate IP(3)-mediated Ca(2+) release from the endoplasmic reticulum. This review summarizes the current state of knowledge regarding the interaction of Bcl-2, and al...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Distelhorst CW,Bootman MD

    更新日期:2011-09-01 00:00:00

  • Effects of redox agents on the Ca2+-activated K+ channel.

    abstract::The sensitivity to Ca2+ of the Ca2+-dependent K+ channel can be increased by the artificial electron donor system ascorbate + phenazine-methosulphate in a variety of animal cells. In the human erythrocyte the shift from the 'low' to the 'high-affinity' state seems to depend on the reduction of a membrane component acc...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: García-Sancho J,Herreros B

    更新日期:1983-12-01 00:00:00

  • Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching.

    abstract::Both theoretical and experimental results are presented for in vivo calibration of the dissociation constant K(Ca)(d)of the calcium-sensitive fluorescent dye Rhod(2)in the perfused mouse heart, using manganese quenching of fluorescence transients. An analytical model is derived, based on the biochemical equilibrium of...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Du C,MacGowan GA,Farkas DL,Koretsky AP

    更新日期:2001-04-01 00:00:00

  • Calcium store depletion activates two distinct calcium entry pathways in secretory cells of the blowfly salivary gland.

    abstract::Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Zimmermann B

    更新日期:1998-01-01 00:00:00

  • Interactions between inositol trisphosphate receptors and fluorescent Ca2+ indicators.

    abstract::Fura-2 and BAPTA were previously shown to be competitive antagonists of inositol trisphosphate (InsP3) receptors, but for practical reasons the analyses were performed at pH 8.3. We recently developed a scintillation proximity assay (SPA) for pure cerebellar InsP3 receptors which allows low affinity interactions to be...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Morris SA,Correa V,Cardy TJ,O'Beirne G,Taylor CW

    更新日期:1999-02-01 00:00:00

  • One nuclear calcium transient induced by a single burst of action potentials represents the minimum signal strength in activity-dependent transcription in hippocampal neurons.

    abstract::Neurons undergo dramatic changes in their gene expression profiles in response to synaptic stimulation. The coupling of neuronal excitation to gene transcription is well studied and is mediated by signaling pathways activated by cytoplasmic and nuclear calcium transients. Despite this, the minimum synaptic activity re...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Yu Y,Oberlaender K,Bengtson CP,Bading H

    更新日期:2017-07-01 00:00:00

  • Intracellular concentrations of fura-2 and fura-2/am in vascular smooth muscle cells following perfusion loading of fura-2/am in arterial segments.

    abstract::A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 n...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Tran NN,Leroy P,Bellucci L,Robert A,Nicolas A,Atkinson J,Capdeville-Atkinson C

    更新日期:1995-11-01 00:00:00

  • Kinetics of inhibition of the plasma membrane calcium pump by vanadate in intact human red cells.

    abstract::The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Tiffert T,Lew VL

    更新日期:2001-11-01 00:00:00

  • Purinoceptor P2U identification and function in human intrahepatic biliary epithelial cell lines.

    abstract::The mechanisms that regulate ion and fluid transport by the human intrahepatic bile duct have not been well defined. Human intrahepatic biliary cell lines that we have developed were used to identify and characterize purinoceptors based on increases in intracellular calcium in response to ATP and other nucleotides. In...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Wolkoff LI,Perrone RD,Grubman SA,Lee DW,Soltoff SP,Rogers LC,Beinborn M,Fang SL,Cheng SH,Jefferson DM

    更新日期:1995-05-01 00:00:00

  • Vasopressin-induced Ca(2+) signals in human adipose-derived stem cells.

    abstract::Intracellular Ca(2+) signals are essential for stem cell differentiation due to their ability to control signaling pathways involved in this process. Arginine vasopression (AVP) is a neurohypophyseal hormone that increases intracellular Ca(2+) concentration during adipogenesis via V1a receptors, Gq-proteins and the PL...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Tran TD,Gimble JM,Cheng H

    更新日期:2016-03-01 00:00:00

  • Activation of the nitric oxide/cGMP pathway is required for refilling intracellular Ca2+ stores in a sympathetic neuron cell line.

    abstract::Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Harrington MA,Thompson SH

    更新日期:1996-05-01 00:00:00

  • Inhibition of ADP-induced intracellular Ca2+ responses and platelet aggregation by the P2Y12 receptor antagonists AR-C69931MX and clopidogrel is enhanced by prostaglandin E1.

    abstract::P2Y(12) antagonists such as clopidogrel and AR-C69931MX inhibit aggregation by antagonizing the effects of ADP at P2Y(12) receptors on platelets. Agents such as PGE(1) also inhibit aggregation by stimulating adenylate cyclase to produce cAMP, which interferes with Ca(2+) mobilization within the cell. Since one facet o...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Fox SC,Behan MW,Heptinstall S

    更新日期:2004-01-01 00:00:00

  • Pore opening mechanism of CRAC channels.

    abstract::Three decades ago, James W. Putney Jr. conceptualized the idea of store-operated calcium entry (SOCE) to explain how depletion of endoplasmic reticulum (ER) Ca2+ stores evokes Ca2+ influx across the plasma membrane. Since the publication of this highly influential idea, it is now established that SOCE is universal amo...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Yeung PS,Yamashita M,Prakriya M

    更新日期:2017-05-01 00:00:00

  • Ultraviolet light and photosensitising agents activate TRPA1 via generation of oxidative stress.

    abstract::TRPA1, a Ca(2+)-permeable cation channel that is expressed in sensory neurones, is involved in the perception of chemical irritants and mechanical hyperalgesia. TRPA1 is activated by either covalent or reversible binding of various chemical compounds, including allylisothiocyanate or acrolein, and is further sensitise...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Hill K,Schaefer M

    更新日期:2009-02-01 00:00:00

  • Control of photoreceptor proteins by Ca2+.

    abstract::A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Koch KW

    更新日期:1995-10-01 00:00:00

  • Theoretical analysis of calcium wave propagation based on inositol (1,4,5)-trisphosphate (InsP3) receptor functional properties.

    abstract::In the presence of inositol (1,4,5)-trisphosphate (InsP3) repetitive waves of elevated cytosolic free Ca2+ (Ca waves) that travel through cellular cytoplasm are observed. Investigation of this phenomenon stimulated the view of cellular cytoplasm as 'an excitable medium composed of Ca release processes (InsP3R), couple...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Bezprozvanny I

    更新日期:1994-09-01 00:00:00

  • Estradiol modulation of PMA--and ionomycin-stimulated LH secretion from pituitaries of castrated rats.

    abstract::Quartered pituitaries from castrated (72 h) +/- estradiol (E2)-treated (24 h) rats were used in a perifusion system to investigate estradiol modulation of ionomycin and ionomycin + PMA stimulated LH secretion. Estradiol enhanced the LH responses to GnRH (1 nM) and ionomycin (10 microM), and was necessary for the manif...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Fahmy NW,Bourne GA

    更新日期:1993-01-01 00:00:00

  • X-ray microprobe analysis of epithelial calcium transport.

    abstract::The sternal epithelium of Porcellio scaber was used as a novel model to study the subcellular elemental distribution in control and Ca(2+)-transporting stages in situ. The anterior sternal epithelium (ASE) is specialized for transport of cuticular Ca to sternal CaCO(3) deposits during premolt, and from these deposits ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Ziegler A

    更新日期:2002-06-01 00:00:00

  • Analysis of cellular calcium fluxes in cardiac muscle to understand calcium homeostasis in the heart.

    abstract::Central to controlling intracellular calcium concentration ([Ca(2+)](i)) are a number of Ca(2+) transporters and channels with the L-type Ca(2+) channel, Na(+)-Ca(2+) exchanger and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) being of particular note in the heart. This review concentrates on the regulation of [Ca(2+)]...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Dibb KM,Graham HK,Venetucci LA,Eisner DA,Trafford AW

    更新日期:2007-10-01 00:00:00