Abstract：
:Signal transduction by auditory and vestibular hair cells involves an impressive ensemble of finely tuned control mechanisms, strictly dependent on the local intracellular Ca(2+) concentration ([Ca(2+)](i)). The study of Ca(2+) dynamics in hair cells typically combines Ca(2+)-sensitive fluorescent indicators (dyes), patch clamp and optical microscopy to produce images of the patterns of fluorescence of a Ca(2+) indicator following various stimulation protocols. Here we describe a novel method that combines electrophysiological recordings, fluorescence imaging and numerical simulations to effectively deconvolve Ca(2+) signals within cytoplasmic microdomains that would otherwise remain inaccessible to direct observation. The method relies on the comparison of experimental data with virtual signals derived from a Monte Carlo reaction-diffusion model based on a realistic reconstruction of the relevant cell boundaries in three dimensions. The model comprises Ca(2+) entry at individual presynaptic active zones followed by diffusion, buffering, extrusion and release of Ca(2+). Our results indicate that changes of the hair cell [Ca(2+)](i) during synaptic transmission are primarily controlled by the Ca(2+) endogenous buffers both at short (<1mu) and at long (tens of microns) distances from the active zones. We provide quantitative estimates of concentration and kinetics of the hair cell endogenous Ca(2+) buffers and Ca(2+)-ATPases. We finally show that experimental fluorescence data collected during Ca(2+) influx are not interpreted correctly if the [Ca(2+)](i) is estimated by assuming that Ca(2+) equilibrates instantly with its reactants. In our opinion, this approach is of potentially general interest as it can be easily adapted to the study of Ca(2+) dynamics in diverse biological systems.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Bortolozzi M,Lelli A,Mammano Fdoi
10.1016/j.ceca.2007.11.007subject
Has Abstractpub_date
2008-08-01 00:00:00pages
158-68issue
2eissn
0143-4160issn
1532-1991pii
S0143-4160(07)00187-Xjournal_volume
44pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Ca(2+) influx through voltage-gated Ca(2+) channels triggers the release of neurotransmitters at presynaptic terminals. Some sensory receptor cells in the peripheral auditory and visual systems have specialized synapses that express an electron-dense organelle called a synaptic ribbon. Like conventional synapses, ribb...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.06.004
更新日期：2012-09-01 00:00:00
abstract：:Store-operated Ca2+ entry (SOCE), an important Ca2+ signaling pathway in non-excitable cells, regulates a variety of cellular functions. To study its physiological role, pharmacological tools, like 2-aminoethyl diphenylborinate (2-APB), are used to impact SOCE. 2-APB is one of the best characterized SOCE inhibitors. H...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2017.01.015
更新日期：2017-03-01 00:00:00
abstract：:Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90113-x
更新日期：1996-05-01 00:00:00
abstract：:Palmitoylation (S-acylation) is the reversible conjugation of a fatty acid (usually C16 palmitate) to intracellular cysteine residues of proteins via a thioester linkage. Palmitoylation anchors intracellular regions of proteins to membranes because the palmitoylated cysteine is recruited to the lipid bilayer. NCX1 is ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.102158
更新日期：2020-03-01 00:00:00
abstract：:Calcium ion is involved in diverse physiological and developmental pathways. One of the important roles of calcium is a signaling messenger, which regulates signal transduction in plants. CBL (calcineurin B-like protein) is one of the calcium sensors that specifically interact with a family of serine-threonine protein...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.05.003
更新日期：2014-08-01 00:00:00
abstract：:Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca2+ mobilizing second messenger which triggers Ca2+ release in both sea urchin egg homogenates and in mammalian cells. The NAADP binding protein has not been identified and the regulation of NAADP mediated Ca2+ release remains controversial. To address...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.102060
更新日期：2019-11-01 00:00:00
abstract：:Cellular redox status is maintained by the balance between series of antioxidant systems and production of reactive oxygen/nitrogenous species. Cells utilize this redox balance to mediate diverse physiological functions. Transient receptor potential (TRP) channels are non-selective cation channels that act as biosenso...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.02.009
更新日期：2016-08-01 00:00:00
abstract：:Ca2+ signalling governs stimulated exocytosis and exocytosis-coupled endocytosis also in Paramecium cells. Upon stimulation, the < or =10(3) dense-core exocytotic organelles (trichocysts) can be synchronously (80 ms) released, followed by endocytotic membrane resealing (350 ms) and retrieval. Paramecium is the most sy...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2005.06.008
更新日期：2005-09-01 00:00:00
abstract：:One of the important events during hypoxia or ischemia in the brain (or other organs for that matter, including the myocardium) is the accumulation of Ca2+ ions intracellularly. Although various studies have shown various sources of and routes for Ca2+ entry and accumulation, it is clear now that it is likely that the...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.02.013
更新日期：2004-09-01 00:00:00
abstract：:In the presence of inositol (1,4,5)-trisphosphate (InsP3) repetitive waves of elevated cytosolic free Ca2+ (Ca waves) that travel through cellular cytoplasm are observed. Investigation of this phenomenon stimulated the view of cellular cytoplasm as 'an excitable medium composed of Ca release processes (InsP3R), couple...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90019-1
更新日期：1994-09-01 00:00:00
abstract：:The annexins are a family of calcium-binding proteins. Data from protein and cDNA sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. The proteins are pres...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(89)90038-9
更新日期：1989-01-01 00:00:00
abstract：:A 106 kD protein was isolated from skeletal sarcoplasmic reticulum (SR) vesicles and shown to have the properties of SR Ca2+ release channels, including blockade by 5 nM ryanodine. In view of extensive reports that the ryanodine-receptor complex consists of four 565 kD junctional feet proteins (JFPs) and is the 'physi...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90074-3
更新日期：1992-11-01 00:00:00
abstract：:Great insight has been gained into the structure and function of the inositol 1,4,5 trisphosphate receptor (InsP(3)R) by studies employing mutagenesis of the cDNA encoding the receptor. Notably, early studies using this approach defined the key constituents required for InsP(3) binding in the N-terminus and the membra...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2010.04.005
更新日期：2010-06-01 00:00:00
abstract：:Using single-cell ratio imaging of Fura-2-loaded neutrophils, we demonstrate that the heterogeneity and asynchrony of the oxidase response originates from variability in the timing and magnitude of the cytosolic free Ca2+ signal. The Ca2+ signals from individual cells could be classified into four types: (a) type 1, a...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90020-u
更新日期：1990-11-01 00:00:00
abstract：:The regulation of intracellular Ca(2+) signalling by phosphorylation processes remains poorly defined, particularly with regards to tyrosine phosphorylation. Evidence from non-excitable cells implicates tyrosine phosphorylation in the activation of so-called store-operated Ca(2+) channels (SOCCs), but their involvemen...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:Studies of Ca2+ transport pathways in exocrine gland cells have been useful, chiefly because of the polarized nature of the secretory epithelial cells. In pancreatic acinar cells, for example, Ca2+ reloading of empty intracellular stores can occur solely via Ca2+ entry through the basal part of the plasma membrane. On...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00047-2
更新日期：2003-05-01 00:00:00
abstract：:There is controversy as to whether TRP channels participate in mediating store-operated current (I(SOC)) and store-operated Ca(2+) entry (SOCE). Our recent study has demonstrated that TRPC1 forms heteromeric channels with TRPV4 in vascular endothelial cells and that Ca(2+) store depletion enhances the vesicle traffick...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.08.006
更新日期：2011-12-01 00:00:00
abstract：:Calcium (Ca2+) signaling controls a wide range of cellular processes, including the hallmarks of cancer. The Ca2+ signaling system encompasses several types of proteins, such as receptors, channels, pumps, exchangers, buffers, and sensors, of which several are mutated or with altered expression in cancer cells. Since ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2020.102285
更新日期：2020-11-01 00:00:00
abstract：:MiR-204 is expressed in vascular smooth muscle cells (VSMC). However, its role in VSMC contraction is not known. We determined if miR-204 controls VSMC contractility and blood pressure through regulation of sarcoplasmic reticulum (SR) calcium (Ca2+) release. Systolic blood pressure (SBP) and vasoreactivity to VSMC con...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.03.006
更新日期：2019-06-01 00:00:00
abstract：:A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 n...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90057-8
更新日期：1995-11-01 00:00:00
abstract：:Several potentially life-threatening cardiovascular and respiratory disorders result in prolonged deprivation of oxygen, which in turn results in significant cellular adaptation, or remodelling. An important component of this functional adaptation arises as a direct consequence of altered ion channel expression by chr...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.02.005
更新日期：2004-09-01 00:00:00
abstract：:The store-operated, calcium release-activated calcium current I(CRAC) is activated by the depletion of inositol 1,4,5-trisphosphate (IP(3))-sensitive stores. The significantly different dose-response relationships of IP(3)-mediated Ca(2+) release and CRAC channel activation indicate that I(CRAC) is activated by a func...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.12.001
更新日期：2009-04-01 00:00:00
abstract：:As pivotal players in cellular metabolism, mitochondria have a double-faceted role in the final decision of cell fate. This is true for all cell types, but it is even more important and intriguing in the cancer setting. Mitochondria regulate cell fate in many diverse ways: through metabolism, by producing ATP and othe...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2020.102308
更新日期：2020-12-01 00:00:00
abstract：:An influx of Ca2+ into red blood cells of the bivalve mollusc Noetia ponderosa occurs immediately following a hypo-osmotic stress. The volume recovery response to the stress is dependent upon [Ca2+]o and is inhibited by phenothiazines. The action of these drugs is on the amino acid regulation portion of the recovery r...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(89)90070-5
更新日期：1989-04-01 00:00:00
abstract：:Dopamine is one of the key neurotransmitters in the central nervous system and plays an important role in physiological processes, as well as in the development of many diseases. Here we report a receptor-independent signalling pathway induced by dopamine in CNS neurons. In cultured neurons from midbrain, cortex and h...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2010.08.008
更新日期：2010-08-01 00:00:00
abstract：:The effect of Ca2+ ion concentration on the 25 hydroxylation of tritiated cholecalciferol (3HD3) was investigated using homogenates of ovine liver from vitamin D replete sheep. A significant decrease in the production of 25 hydroxycholecalciferol (25OHD3) was observed when the concentration of Ca2+ in the homogenate w...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(87)90001-7
更新日期：1987-08-01 00:00:00
abstract：:The secretion of adrenocorticotrophin (ACTH) from corticotropes is a key component in the endocrine response to stress. The resting potential of corticotropes is set by the basal activities of TWIK-related K(+) (TREK)-1 channel. Corticotrophin-releasing hormone (CRH), the major ACTH secretagogue, closes the background...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.12.007
更新日期：2012-03-01 00:00:00
abstract：:Receptors for extracellular nucleotides (the P2X-calcium channels and the phospholipase C-coupled P2Y receptors) play key roles in pain signaling, but little is known on their function in trigeminal ganglia, whose hyperactivation leads to the development of migraine pain. Here we characterize calcium signaling via P2X...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.10.003
更新日期：2008-06-01 00:00:00
abstract：:TRP family of proteins are components of unique cation channels that are activated in response to diverse stimuli ranging from growth factor and neurotransmitter stimulation of plasma membrane receptors to a variety of chemical and sensory signals. This review will focus on members of the TRPC sub-family (TRPC1-TRPC7)...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.011
更新日期：2006-11-01 00:00:00
abstract：:We studied the contribution of store-operated or capacitative Ca2+-entry (SOCE or CCE, respectively) through store-operated Ca2+ channels (SOCCs) and the contribution of Ca2+-entry through receptor-operated, non-selective cation channels (ROCCs or NSCCs, respectively), on the M3-receptor-mediated (270 nM Ach) contract...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2005.06.035
更新日期：2005-11-01 00:00:00
