Contribution of capacitative and non-capacitative Ca2+-entry to M3-receptor-mediated contraction of porcine coronary smooth muscle.

Abstract:

:We studied the contribution of store-operated or capacitative Ca2+-entry (SOCE or CCE, respectively) through store-operated Ca2+ channels (SOCCs) and the contribution of Ca2+-entry through receptor-operated, non-selective cation channels (ROCCs or NSCCs, respectively), on the M3-receptor-mediated (270 nM Ach) contractile response of porcine coronary smooth muscle strips by means of the respective inhibitors. In the presence of L-VOCC blockade (1 microM verapamil), LOE 908 (inhibition of NSCCs) decreased the contractile response to 75+/-5% (p<0.01, n=6), 2-APB (inhibition of SOCCs) and SK and F 96365 (inhibition of SOCCs and of NSCCs) decreased the response to 45+/-4% (p<0.001, n=10) and to 23+/-2% (p<0.001, n=5), respectively (control: Ach response in the presence of verapamil alone). In the absence of L-VOCC blockade, LOE 908 reduced the Ach-response to 49+/-7% (p<0.001, n=8) and SK and F 96365 to 3+/-2% (p<0.001, n=4) of control, whereas 2-APB transiently increased the response (peak effect: 130+/-11%; p<0.05, n=8). We conclude: (1) the main source of activator Ca2+ during the M3-receptor-mediated contractile response is the Ca2+ influx through L-VOCCs; (2) however, in the presence of L-VOCC blockade, the contractile response is mainly due to Ca2+-entry through SOCCs; (3) NSCCs may be considerably involved in M3-receptor-mediated contraction as they may serve to depolarize the membrane potential and, thus, to open L-VOCCs; (4) in primary tissue of vascular smooth muscle, both, SOCE and Ca2+-entry through NSCCs are activated during M3-receptor stimulation.

journal_name

Cell Calcium

journal_title

Cell calcium

authors

Weirich J,Dumont L,Fleckenstein-Grün G

doi

10.1016/j.ceca.2005.06.035

keywords:

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

457-67

issue

5

eissn

0143-4160

issn

1532-1991

pii

S0143-4160(05)00123-5

journal_volume

38

pub_type

杂志文章
  • Testosterone sensitive dihydropyridine binding in the Harderian gland of the male hamster.

    abstract::It is well known that in different tissues, dihydropyridines bind at nanomolar concentrations to a receptor and block voltage-operated Ca2+ channels. In studies reported here, Harderian gland tissue homogenates from intact male hamsters exhibited significant dihydropyridine binding (Bmax = 1700 fmoles/mg protein) of h...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/0143-4160(92)90036-r

    authors: Kumar P,Brodie SG,Vaughan MK,Menendez-Pelaez A,Reiter RJ,Chambers JP

    更新日期:1992-10-01 00:00:00

  • Enhanced spontaneous Ca2+ events in endothelial cells reflect signalling through myoendothelial gap junctions in pressurized mesenteric arteries.

    abstract::Increases in global Ca(2+) in the endothelium are a crucial step in releasing relaxing factors to modulate arterial tone. In the present study we investigated spontaneous Ca(2+) events in endothelial cells, and the contribution of smooth muscle cells to these Ca(2+) events, in pressurized rat mesenteric resistance art...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2007.11.012

    authors: Kansui Y,Garland CJ,Dora KA

    更新日期:2008-08-01 00:00:00

  • Caloxin 1b3: a novel plasma membrane Ca(2+)-pump isoform 1 selective inhibitor that increases cytosolic Ca(2+) in endothelial cells.

    abstract::The purpose of this study was to invent an extracellular inhibitor selective for the plasma membrane Ca(2+) pump(s) (PMCA) isoform 1. PMCA extrude Ca(2+) from cells during signalling and homeostasis. PMCA isoforms are encoded by 4 genes (PMCA1-4). Pig coronary artery endothelium and smooth muscle express the genes PMC...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2010.10.008

    authors: Szewczyk MM,Pande J,Akolkar G,Grover AK

    更新日期:2010-12-01 00:00:00

  • Calcium signaling and cellular senescence.

    abstract::Cellular senescence is a stable cell proliferation arrest induced by a variety of stresses including telomere shortening, oncogene activation and oxidative stress. This process plays a crucial role in many physiopathological contexts, especially during aging when cellular senescence favors development of age-related d...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2017.04.001

    authors: Martin N,Bernard D

    更新日期:2018-03-01 00:00:00

  • Reassessment of Fura-2 and the ratio method for determination of intracellular Ca2+ concentrations.

    abstract::To determine intracellular Ca2+ concentrations more accurately, we examined Kd of Fura-2 for Ca2+ in conditions which were systemically changed. In a solution comprising of 150 mM KCI, 20 mM MOPS-KOH, pH 6.94, 60-100 microM EGTA and 1 microM Fura-2, Kd at 20 degrees C was 0.266 +/- 0.016 microM (mean +/- SEM) (21 dete...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/0143-4160(91)90082-p

    authors: Uto A,Arai H,Ogawa Y

    更新日期:1991-01-01 00:00:00

  • Epileptic activity during early postnatal life in the AY-9944 model of atypical absence epilepsy.

    abstract::Atypical absence epilepsy (AAE) is an intractable disorder characterized by slow spike-and-wave discharges in electroencephalograms (EEGs) and accompanied by severe cognitive dysfunction and neurodevelopmental or neurological deficits in humans. Administration of the cholesterol biosynthesis inhibitor AY-9944 (AY) dur...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2015.04.001

    authors: Jung S,Jeong Y,Jeon D

    更新日期:2015-05-01 00:00:00

  • Spontaneous calcium release from the sarcoplasmic reticulum in myocardial cells: mechanisms and consequences.

    abstract::Under certain conditions of Ca2+ loading, cardiac myocytes, both isolated and in intact tissue, exhibit spontaneous, oscillatory Ca2+ transients due to Ca2+ release from the sarcoplasmic reticulum. These transients are not triggered by depolarization of the sarcolemma, though they themselves can generate depolarizing ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/0143-4160(88)90005-x

    authors: Stern MD,Capogrossi MC,Lakatta EG

    更新日期:1988-12-01 00:00:00

  • Tacrolimus (FK506) modulates calcium release and contractility of intestinal smooth muscle.

    abstract::Several proteins have been identified that associate with calcium release channels and potentially regulate their function. Using tacrolimus as a pharmacological tool, we investigated whether the immunophilin FKBP12 modulates ryanodine receptor channels in intestinal smooth muscle. Results with PCR demonstrated the pr...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/s0143-4160(97)90078-6

    authors: Bielefeldt K,Sharma RV,Whiteis C,Yedidag E,Abboud FM

    更新日期:1997-12-01 00:00:00

  • Polycystin-2 accelerates Ca2+ release from intracellular stores in Caenorhabditis elegans.

    abstract::Polycystin-2, a member of the TRP family of calcium channels, is encoded by the human PKD2 gene. Mutations in that gene can lead to swelling of nephrons into the fluid-filled cysts of polycystic kidney disease. In addition to expression in tubular epithelial cells, human polycystin-2 is found in muscle and neuronal ce...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2005.03.003

    authors: Koulen P,Duncan RS,Liu J,Cohen NE,Yannazzo JA,McClung N,Lockhart CL,Branden M,Buechner M

    更新日期:2005-06-01 00:00:00

  • Two EF-hand motifs in ryanodine receptor calcium release channels contribute to isoform-specific regulation by calmodulin.

    abstract::The mammalian ryanodine receptor Ca2+ release channel (RyR) has a single conserved high affinity calmodulin (CaM) binding domain. However, the skeletal muscle RyR1 is activated and cardiac muscle RyR2 is inhibited by CaM at submicromolar Ca2+. This suggests isoform-specific domains are involved in RyR regulation by Ca...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2017.05.013

    authors: Xu L,Gomez AC,Pasek DA,Meissner G,Yamaguchi N

    更新日期:2017-09-01 00:00:00

  • TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts.

    abstract::Odontoblasts are involved in the transduction of stimuli applied to exposed dentin. Although expression of thermo/mechano/osmo-sensitive transient receptor potential (TRP) channels has been demonstrated, the properties of TRP vanilloid 1 (TRPV1)-mediated signaling remain to be clarified. We investigated physiological ...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2012.05.002

    authors: Tsumura M,Sobhan U,Muramatsu T,Sato M,Ichikawa H,Sahara Y,Tazaki M,Shibukawa Y

    更新日期:2012-08-01 00:00:00

  • A new role for T-type channels in fast "low-threshold" exocytosis.

    abstract::Evidence is accumulating on a key role of T-type channels in neurotransmitter release. Recent works have brought undisputable proofs that T-type channels are capable of controlling hormone and neurotransmitters release in association with exocytosis of large dense-core and synaptic vesicles. T-type channel-secretion c...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2006.04.019

    authors: Carbone E,Giancippoli A,Marcantoni A,Guido D,Carabelli V

    更新日期:2006-08-01 00:00:00

  • Stimulus-dependent changes in calcium metabolism in rabbit neutrophils.

    abstract::We have found that the changes in calcium metabolism in rabbit neutrophils produced by the chemotactic synthetic peptide f-Met-Leu-Phe are not sensitive to the calcium chelator EGTA. The present results demonstrate unambiguously that the previously described chemotactic factor induced changes in 45Ca fluxes in rabbit ...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/0143-4160(83)90049-0

    authors: Molski TF,Naccache PH,Sha'afi RI

    更新日期:1983-02-01 00:00:00

  • Endoplasmic reticulum, Bcl-2 and Ca2+ handling in apoptosis.

    abstract::In the complex signalling interplay that allows extracellular signals to be decoded into activation of apoptotic cell death, Ca(2+) plays a significant role. This is supported not only by evidence linking alterations in Ca(2+) homeostasis to the triggering of apoptotic (and in some cases necrotic) cell death, but also...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/s0143416002002014

    authors: Ferrari D,Pinton P,Szabadkai G,Chami M,Campanella M,Pozzan T,Rizzuto R

    更新日期:2002-11-01 00:00:00

  • Evolution of the Ca2+ current during dialysis of isolated bovine chromaffin cells: effect of internal calcium.

    abstract::We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% with...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/0143-4160(94)90029-9

    authors: Elhamdani A,Bossu JL,Feltz A

    更新日期:1994-11-01 00:00:00

  • Properties of intra- and intercellular Ca(2+)-wave propagation elicited by mechanical stimulation in cultured RPE cells.

    abstract::Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1054/ceca.1999.0044

    authors: Stalmans P,Himpens B

    更新日期:1999-06-01 00:00:00

  • Bcl-2 interaction with the inositol 1,4,5-trisphosphate receptor: role in Ca(2+) signaling and disease.

    abstract::The Bcl-2 protein, best known for its ability to inhibit apoptosis, interacts with the inositol 1,4,5-trisphosphate receptor (IP(3)R) Ca(2+) channel to regulate IP(3)-mediated Ca(2+) release from the endoplasmic reticulum. This review summarizes the current state of knowledge regarding the interaction of Bcl-2, and al...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2011.05.011

    authors: Distelhorst CW,Bootman MD

    更新日期:2011-09-01 00:00:00

  • Molecular determinants of TRPC1 regulation within ER-PM junctions.

    abstract::Store-operated calcium entry (SOCE) is activated within the endoplasmic reticulum (ER)-plasma membrane (PM) junctions in the plasma membrane and involves assembly of the channels, Orai1 and TRPC1, with the regulatory protein, STIM1, in response to depletion of Ca(2+) in the ER. The dynamic assembly of the channel comp...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2015.03.008

    authors: Ong HL,Ambudkar IS

    更新日期:2015-10-01 00:00:00

  • Localized calcium signalling and neuronal integration in cerebellar Purkinje neurones.

    abstract::The use of high resolution imaging techniques has revealed new forms of dendritic signal integration in neurones. In contrast to electrical signals that have a more widespread influence on the cell, brief Ca2+ transients resulting from synaptic activation are often restricted to a small part of the dendritic tree. In ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/s0143-4160(96)90108-6

    authors: Eilers J,Plant T,Konnerth A

    更新日期:1996-08-01 00:00:00

  • Ion channel regulation by chronic hypoxia in models of acute oxygen sensing.

    abstract::Several potentially life-threatening cardiovascular and respiratory disorders result in prolonged deprivation of oxygen, which in turn results in significant cellular adaptation, or remodelling. An important component of this functional adaptation arises as a direct consequence of altered ion channel expression by chr...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2004.02.005

    authors: Peers C,Kemp PJ

    更新日期:2004-09-01 00:00:00

  • cAMP increases the sensitivity of exocytosis to Ca²+ primarily through protein kinase A in mouse pancreatic beta cells.

    abstract::Cyclic AMP regulates the late step of Ca²+-dependent exocytosis in many secretory cells through two major mechanisms: a protein kinase A-dependent and a cAMP-GEF/Epac-dependent pathway. We designed a protocol to characterize the role of these two cAMP-dependent pathways on the Ca²+ sensitivity and kinetics of regulate...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2010.12.005

    authors: Skelin M,Rupnik M

    更新日期:2011-02-01 00:00:00

  • Synthesis of vesicle cargo determines amplitude of Ca(2+)-sensitive exocytosis.

    abstract::Here we examine the potential coupling between the synthesis of secretory proteins and the sensitivity of exocytosis to the concentration of free Ca(2+) in the cytosol ([Ca(2+)](i)) in plant cell. We therefore monitor in tobacco protoplasts the excursion of the membrane capacitance in response to an elevation of [Ca(2...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2012.05.011

    authors: Sutter JU,Denecke J,Thiel G

    更新日期:2012-09-01 00:00:00

  • Multi-scale data-driven modeling and observation of calcium puffs.

    abstract::The spatiotemporal dynamics of elementary Ca(2+) release events, such as "blips" and "puffs" shapes the hierarchal Ca(2+) signaling in many cell types. Despite being the building blocks of Ca(2+) patterning, the mechanism responsible for the observed properties of puffs, especially their termination is incompletely un...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2012.04.018

    authors: Ullah G,Parker I,Mak DO,Pearson JE

    更新日期:2012-08-01 00:00:00

  • Pore opening mechanism of CRAC channels.

    abstract::Three decades ago, James W. Putney Jr. conceptualized the idea of store-operated calcium entry (SOCE) to explain how depletion of endoplasmic reticulum (ER) Ca2+ stores evokes Ca2+ influx across the plasma membrane. Since the publication of this highly influential idea, it is now established that SOCE is universal amo...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2016.12.006

    authors: Yeung PS,Yamashita M,Prakriya M

    更新日期:2017-05-01 00:00:00

  • [Na(+)] in the subsarcolemmal 'fuzzy' space and modulation of [Ca(2+)](i) and contraction in cardiac myocytes.

    abstract::The strength of the heart beat depends on the amplitude and time course of the transient increase in [Ca(2+)] in the myocytes with each cycle. [Na(+)](i) modulates cardiac contraction through its effect on the Ca(2+) flux through the Na/Ca exchanger. Cardiac excitation-contraction coupling has been postulated to occur...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2004.01.014

    authors: Verdonck F,Mubagwa K,Sipido KR

    更新日期:2004-06-01 00:00:00

  • Calcium and egg activation in Drosophila.

    abstract::In many animals, a rise in intracellular calcium levels is the trigger for egg activation, the process by which an arrested mature oocyte transitions to prepare for embryogenesis. In nearly all animals studied to date, this calcium rise, and thus egg activation, is triggered by the fertilizing sperm. However in the in...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2012.11.008

    authors: Sartain CV,Wolfner MF

    更新日期:2013-01-01 00:00:00

  • Calcium microdomains in regulated exocytosis.

    abstract::Katz and co-workers showed that Ca(2+) triggers exocytosis. The existence of sub-micrometer domains of greater than 100 microM [Ca(2+)](i) was postulated on theoretical grounds. Using a modified, low-affinity aequorin, Llinas et al. were the first to demonstrate the existence of Ca(2+) 'microdomains' in squid presynap...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/j.ceca.2006.08.007

    authors: Oheim M,Kirchhoff F,Stühmer W

    更新日期:2006-11-01 00:00:00

  • Intracellular Ca2+ and cell injury: a paradoxical role of Ca2+ in complement membrane attack.

    abstract::Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审

    doi:10.1016/0143-4160(86)90042-4

    authors: Morgan BP,Luzio JP,Campbell AK

    更新日期:1986-12-01 00:00:00

  • Ca2+ channel currents and contraction in CaVbeta3-deficient ileum smooth muscle from mouse.

    abstract::Voltage activated L-type Ca(2+) channels are the principal Ca(2+) channels in intestinal smooth muscle cells. They comprise the ion conducting Ca(V)1 pore and the ancillary subunits alpha(2)delta and beta. Of the four Ca(V)beta subunits Ca(V)beta(3) is assumed to be the relevant Ca(V)beta protein in smooth muscle. In ...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2007.04.013

    authors: Held B,Tsvilovskyy V,Meissner M,Kaestner L,Ludwig A,Mossmang S,Lipp P,Freichel M,Flockerzi V

    更新日期:2007-10-01 00:00:00

  • Amyloid β peptide oligomers directly activate NMDA receptors.

    abstract::Amyloid beta (Aβ) oligomers accumulate in the brain tissue of Alzheimer disease patients and are related to disease pathogenesis. The precise mechanisms by which Aβ oligomers cause neurotoxicity remain unknown. We recently reported that Aβ oligomers cause intracellular Ca(2+) overload and neuronal death that can be pr...

    journal_title:Cell calcium

    pub_type: 杂志文章

    doi:10.1016/j.ceca.2011.02.001

    authors: Texidó L,Martín-Satué M,Alberdi E,Solsona C,Matute C

    更新日期:2011-03-01 00:00:00