Abstract：
:To elucidate the regulatory pathway through which pancreastatin inhibits insulin secretion, RINm5F insulinoma cells were challenged with physiological and pharmacological probes known to stimulate insulin release through different mechanisms. Utilizing the electrophysiological technique of capacitance measurements as a correlate to exocytosis, pancreastatin was found to significantly diminish maximum capacitance changes evoked by glyceraldehyde, an effect which was attenuated in pertussis toxin-treated cells. In static incubations of this cell line, pancreastatin significantly inhibited insulin secretion stimulated by glyceraldehyde, carbachol and A23187, secretagogues known to directly elevate beta-cell cytosolic Ca2+. This peptide also inhibited insulin secretion stimulated by phorbol myristate acetate (PMA), but only at incubation times < or = 15 min. It was without effect on insulin secretion stimulated by mastoparan and longer incubations (30 min) with PMA, where the secretory mechanisms are not necessarily Ca(2+)-dependent. Additionally, pancreastatin had no effect on carbachol-generated inositol phosphate accumulation but inhibited simultaneously stimulated insulin secretion. All inhibitory effects of pancreastatin were pertussis toxin sensitive. These results suggest that pancreastatin inhibits insulin secretion in RINm5F cells through a G-protein regulated mechanism at a control point involved in the Ca(2+)-directed exocytotic machinery, a feature shared by other physiologic inhibitors of insulin secretion.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Hertelendy ZI,Patel DG,Knittel JJdoi
10.1016/s0143-4160(96)90081-0subject
Has Abstractpub_date
1996-02-01 00:00:00pages
125-32issue
2eissn
0143-4160issn
1532-1991pii
S0143-4160(96)90081-0journal_volume
19pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Dynamic nuclear Ca(2+) signals play pivotal roles in diverse cellular functions including gene transcription, cell growth, differentiation, and apoptosis. Here we report a novel nuclear Ca(2+) regulatory mechanism mediated by inositol 1,4,5-trisphosphate receptors (IP(3)Rs) around the nucleus in developing cardiac myo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.04.017
更新日期：2008-02-01 00:00:00
abstract：:The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2001.0233
更新日期：2001-10-01 00:00:00
abstract：:T-type Ca(2+) channel family includes three subunits Ca(V)3.1, Ca(V)3.2 and Ca(V)3.3 and have been shown to control burst firing and intracellular Ca(2+) concentration ([Ca(2+)](i)) in neurons. Here, we investigated whether Ca(V)3.1 channels could generate a pacemaker current and contribute to cell excitability. Ca(V)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.04.003
更新日期：2008-12-01 00:00:00
abstract：:Light-sensitive channels encoded by the Drosophila transient receptor potential-like gene (trpl) are activated in situ by an unknown mechanism requiring activation of Gq and phospholipase C (PLC). Recent studies have variously concluded that heterologously expressed TRPL channels are activated by direct Gq-protein int...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90125-7
更新日期：1998-09-01 00:00:00
abstract：:The regulation of intracellular Ca(2+) signalling by phosphorylation processes remains poorly defined, particularly with regards to tyrosine phosphorylation. Evidence from non-excitable cells implicates tyrosine phosphorylation in the activation of so-called store-operated Ca(2+) channels (SOCCs), but their involvemen...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:We report here the first local and global Ca(2+) measurements made from in situ terminal arterioles. The advantages of the method are that there is minimal disturbance to the vessels, which retain their relationship to the tissue they are supplying (rat ureter) and the small size of vessel that can be studied. Good lo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00019-8
更新日期：2003-07-01 00:00:00
abstract：:The best known calcium waves move at about 5-30 microns/s (at 20 degrees C) and will be called fast waves to distinguish them from slow (contractile) ones which move at 0.1-1 microns/s as well as electrically propagated, ultrafast ones. Fast waves move deep within cells and seem to underlie most calcium signals. Their...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(93)90099-r
更新日期：1993-11-01 00:00:00
abstract：:Intracellular Ca(2+)-transport ATPases exert a pivotal role in the endoplasmic reticulum and in the compartments of the cellular secretory pathway by maintaining a sufficiently high lumenal Ca(2+) (and Mn(2+)) concentration in these compartments required for an impressive number of vastly different cell functions. At ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143416002001847
更新日期：2002-11-01 00:00:00
abstract：:Cellular calcium signaling events are transient. Hence they are observed in real time using fluorescence imaging or electrophysiological methods that require sophisticated instrumentation and specialized skills. For high throughput assays simple and inexpensive techniques are desirable. Many calcium channels that serv...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.05.009
更新日期：2018-09-01 00:00:00
abstract：:Katz and co-workers showed that Ca(2+) triggers exocytosis. The existence of sub-micrometer domains of greater than 100 microM [Ca(2+)](i) was postulated on theoretical grounds. Using a modified, low-affinity aequorin, Llinas et al. were the first to demonstrate the existence of Ca(2+) 'microdomains' in squid presynap...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.007
更新日期：2006-11-01 00:00:00
abstract：:K+-dependent Na+-Ca2+ exchangers (NCKXs) belong to Ca2+/cation antiporter gene superfamily. NCKX proteins play an important role in Ca2+ homeostasis and are bi-directional plasma membrane Ca2+-transporters which utilize the inward Na+ and outward K+ gradients to move Ca2+ ions into and out of the cytosol (4Na+:1Ca2+ +...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.06.002
更新日期：2018-09-01 00:00:00
abstract：:Stromal interaction molecule (STIM)-1 and -2 are multi-domain, single-pass transmembrane proteins involved in sensing changes in compartmentalized calcium (Ca2+) levels and transducing this cellular signal to Orai1 channel proteins. Our understanding of the molecular mechanisms underlying STIM signaling has been drama...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:Sarcoplasmic reticulum contains the internal Ca(2+) store in smooth muscle cells and its lumen appears to be a continuum that lacks diffusion barriers. Accordingly, the free luminal Ca(2+) level is the same all throughout the SR; however, whether the Ca(2+) buffer capacity is the same in all the SR is unknown. We have...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.07.005
更新日期：2009-09-01 00:00:00
abstract：:The sternal epithelium of Porcellio scaber was used as a novel model to study the subcellular elemental distribution in control and Ca(2+)-transporting stages in situ. The anterior sternal epithelium (ASE) is specialized for transport of cuticular Ca to sternal CaCO(3) deposits during premolt, and from these deposits ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(02)00060-x
更新日期：2002-06-01 00:00:00
abstract：:MiR-204 is expressed in vascular smooth muscle cells (VSMC). However, its role in VSMC contraction is not known. We determined if miR-204 controls VSMC contractility and blood pressure through regulation of sarcoplasmic reticulum (SR) calcium (Ca2+) release. Systolic blood pressure (SBP) and vasoreactivity to VSMC con...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.03.006
更新日期：2019-06-01 00:00:00
abstract：:When the concentrations of either calcium or of magnesium in the culture medium were increased from the normal 0.6 and 1.0 mM to 1.8 and 2.5 mM respectively mitotic activity of rat thymic lymphocytes increased. Very high (10(-4)M) ouabain concentrations abolished these mitogenic actions whilst lower (10(-7) and 10(-11...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(83)90044-1
更新日期：1983-02-01 00:00:00
abstract：:We investigated whether the expression of human alpha-parvalbumin affects depolarization-induced elevations of the cytosolic free calcium concentration ([Ca2+]i) in human neuroblastoma SKNBE2 cells. A full length human parvalbumin cDNA was cloned by PCR from human cerebellum and transiently transfected into SKNBE2 cel...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90062-7
更新日期：1996-06-01 00:00:00
abstract：:Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0044
更新日期：1999-06-01 00:00:00
abstract：:Incubation of human erythrocyte ghosts with an equal volume of 0.2 mM EDTA in isotonic KCl decreased both the activity and Ca2+ sensitivity of the (Ca2+ + Mg2+)-ATPase remaining associated with the membrane. Readdition of the EDTA-extract activated the (Ca2+ + Mg2+)-ATPase activity. The activator activity was trypsin ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(84)90156-8
更新日期：1984-02-01 00:00:00
abstract：:Parvalbumin (PA) is a Ca(2+)-binding protein of vertebrates massively expressed in tissues with high oxygen uptake and respectively elevated level of reactive oxygen species (ROS). To characterize antioxidant properties of PA, antioxidant capacity (AOC) of intact rat α-PA has been explored. ORAC, TEAC and hydrogen per...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.03.001
更新日期：2014-05-01 00:00:00
abstract：:Specific sequences (cis-acting elements) in the 3'-untranslated region (UTR) of RNA, together with stabilizing and destabilizing proteins (trans-acting factors), determine the mRNA stability, and consequently, the level of expression of several proteins. Such interactions were discovered initially for short-lived mRNA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.004
更新日期：2006-10-01 00:00:00
abstract：:Ca2+ signals are known to mediate an array of cellular functions including secretion, contraction, and conductivity changes. In spite of the obvious role of Ca2+ in signalling, the control of Ca2+ within cells is known to be a complex phenomenon involving a number of distinct active and passive transport systems funct...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(89)90062-6
更新日期：1989-07-01 00:00:00
abstract：:Alternative splicing of the sarco/endoplasmic reticulum (SERCA2) Ca2+ pump transcript generates the two isoforms: SERCA2a in left ventricular myocytes (LVM) and SERCA2b in most tissues. Nuclear protein extracts from left ventricular myocytes can cause a decay of the 3'-region of the SERCA2a. To determine if all the do...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2004.06.003
更新日期：2005-01-01 00:00:00
abstract：:A possible role of the nitric oxide (NO)/cGMP pathway in the regulation of Ca2+ entry into HT29/B6 human colonic epithelial cells was investigated using digital image processing of Fura-2 fluorescence and immunoblotting for nitric oxide synthase (NOS). We tested the hypothesis that Ca2+ store depletion causes increase...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90071-3
更新日期：1995-04-01 00:00:00
abstract：:Calcium-permeable channels have traditionally been thought of as therapeutic targets in excitable cells. For instance, voltage-operated Ca2+ channels in neurones and smooth muscle cells for neurological and cardiovascular diseases although calcium-permeable channels are also functionally important in electrically non-...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00060-5
更新日期：2003-05-01 00:00:00
abstract：:Isolated avian growth plate chondrocytes convert the acetoxymethyl ester (AM) form of Fura-2 quickly and efficiently to the Ca2(+)-sensitive pentacarboxylic acid (FA) form. Control experiments indicate that the Kd for intracellular Fura-2/FA is very close to that of extracellular Fura-2/FA at the same ionic strength a...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90077-8
更新日期：1990-08-01 00:00:00
abstract：:Animals have evolved sophisticated physiological systems for sensing ambient temperature since changes in environmental temperatures affect various biological processes. Thermosensitive transient receptor potential (thermoTRP) channels serve as thermal sensors in diverse animal species. They are multimodal receptors t...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2014.12.001
更新日期：2015-03-01 00:00:00
abstract：:Store-operated calcium entry (SOCE) is activated within the endoplasmic reticulum (ER)-plasma membrane (PM) junctions in the plasma membrane and involves assembly of the channels, Orai1 and TRPC1, with the regulatory protein, STIM1, in response to depletion of Ca(2+) in the ER. The dynamic assembly of the channel comp...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2015.03.008
更新日期：2015-10-01 00:00:00
abstract：:Extracellular nucleotides are potent Ca2+ mobilizing agents. A variety of receptors for extracellular ATP are recognised. Some are involved in fast neuronal transmission and operate as ligand-gated ion channels. Others are involved in the paracrine or autocrine modulation of cell function. Many receptors of this type ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90080-2
更新日期：1995-02-01 00:00:00
abstract：:Rabbit gall-bladder epithelial cells were isolated by a combination of Ca2+ omission, enzymatic treatment, and mechanical detachment and had a viability of 96-98% and well preserved morphology. Measurements of cytosolic free Ca2+ concentration ([Ca2+]i) in these cells with the Ca2+-fluorescent indicator fura-2 demonst...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(89)90042-0
更新日期：1989-01-01 00:00:00
