Abstract：
:Parvalbumin (PA) is a Ca(2+)-binding protein of vertebrates massively expressed in tissues with high oxygen uptake and respectively elevated level of reactive oxygen species (ROS). To characterize antioxidant properties of PA, antioxidant capacity (AOC) of intact rat α-PA has been explored. ORAC, TEAC and hydrogen peroxide AOC assays evidence conformation-dependent oxidation of the PA. AOC value for the apo-PA 4-11-fold exceeds that for the Ca(2+)-loaded protein. Despite folded conformation of apo-PA, it has AOC equivalent to that of the proteolized protein. The most populated under resting conditions PA form, Mg(2+)-bound PA, has AOC similar to that of apo-PA. ROS-induced changes in absorption spectrum of PA evidence an oxidation of PA's phenylalanines in the ORAC assay. Sensitivity of PA oxidation to its conformation enabled characterization of its metal affinity and pH-dependent behavior: a transition with pKa of 7.6 has been revealed for the Ca(2+)-loaded PA. Since total AOC of PA under in vivo conditions may reach the level of reduced glutathione, we propose that PA might modulate intracellular redox equilibria and/or signaling in a calcium-dependent manner. We speculate that the oxidation-mediated damage of some of PA-GABAergic interneurons observed in schizophrenia is due to a decline in total AOC of the reduced glutathione-PA pair.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Permyakov SE,Kazakov AS,Avkhacheva NV,Permyakov EAdoi
10.1016/j.ceca.2014.03.001subject
Has Abstractpub_date
2014-05-01 00:00:00pages
261-8issue
5eissn
0143-4160issn
1532-1991pii
S0143-4160(14)00042-6journal_volume
55pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90074-4
更新日期：1998-01-01 00:00:00
abstract：:Glucose induces large amplitude oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i) in pancreatic beta-cells. The effects of temperature on these oscillations were examined by monitoring [Ca2+]i continuously in single beta-cells from ob/ob-mice using dual wavelength microfluorometry. The oscillations of [Ca2+...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90053-w
更新日期：1990-06-01 00:00:00
abstract：:Calcium and redox signals are presently established as essential regulators of many cellular processes. Nevertheless, we are still far from fully understanding the physiological and pathological importance of these universal second messengers. It is becoming increasingly apparent that many cellular functions are not r...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.04.008
更新日期：2016-08-01 00:00:00
abstract：:Maitotoxin (MTX), a water-soluble polyether obtained from the marine dinoflagellate Gambierdiscus toxicus increased intracellular calcium in a concentration-dependent manner in fibroblasts obtained from human skin. The effect of this toxin was both saturable and of high affinity, showing an apparent half activation co...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90087-7
更新日期：1997-07-01 00:00:00
abstract：:Intercellular Ca(2+)-signaling, after mechanical stimulation of calf pulmonary artery endothelial cells (CPAE), was investigated with fluorescence video imaging. Mechanical stimulation evoked an intracellular Ca(2+)-response in the mechanically stimulated (MS) cell, proceeding to the neighboring (NB) cells as a Ca(2+)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2000.0165
更新日期：2001-02-01 00:00:00
abstract：:Ca(2+) is known as a universal messenger mediating a wide variety of cellular processes, including cell death. In fact, this ion has been proposed as the 'cell death master', not only at the intracellular but also at the intercellular level. The most direct form of intercellular spread of cell death is mediated by gap...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.05.007
更新日期：2011-09-01 00:00:00
abstract：:The store-operated, calcium release-activated calcium current I(CRAC) is activated by the depletion of inositol 1,4,5-trisphosphate (IP(3))-sensitive stores. The significantly different dose-response relationships of IP(3)-mediated Ca(2+) release and CRAC channel activation indicate that I(CRAC) is activated by a func...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.12.001
更新日期：2009-04-01 00:00:00
abstract：:Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brai...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.01.004
更新日期：2014-04-01 00:00:00
abstract：:Recent developments in microscopy and fluorescent indicators now make it possible to monitor the activity and localization of membrane ion channels by imaging Ca(2+) flux through individual channels. Such optical approaches have advantages over electrophysiological single-channel techniques in that they are less invas...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2004.10.008
更新日期：2005-04-01 00:00:00
abstract：:The non-selective second messenger-gated cation channel TRPC6 (transient receptor potential canonical 6) is activated by diacylglycerols (DAG) in a PKC-independent manner and plays important roles in a variety of physiological processes and diseases. In order to facilitate novel therapies, the development of potent in...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.12.009
更新日期：2019-03-01 00:00:00
abstract：:An influx of Ca2+ into red blood cells of the bivalve mollusc Noetia ponderosa occurs immediately following a hypo-osmotic stress. The volume recovery response to the stress is dependent upon [Ca2+]o and is inhibited by phenothiazines. The action of these drugs is on the amino acid regulation portion of the recovery r...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(89)90070-5
更新日期：1989-04-01 00:00:00
abstract：:Cytosolic Ca(2+) signals are fundamental for the early and late steps of myoblast differentiation and are, as in many cells, generated by Ca(2+) release from internal stores as well as by plasma membrane Ca(2+) entry. Our recent studies identified the store-operated Ca(2+) channels, Orai1 and TRPC1&C4, as crucial for ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.10.014
更新日期：2014-12-01 00:00:00
abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2005.06.012
更新日期：2005-09-01 00:00:00
abstract：:The high capacity, low affinity Ca(2+) storage protein chromogranins are marker proteins of secretory granules that contain the most Ca(2+) in secretory cells. Along with the abundantly expressed chromogranins, the IP(3)R/Ca(2+) channels, the major intracellular Ca(2+) channels, are also expressed in secretory granule...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.12.008
更新日期：2012-03-01 00:00:00
abstract：:While genetically encoded Ca(2+) indicators (GECIs) allow Ca(2+) imaging in model organisms, the gene expression is often under the control of a single promoter that may drive expression beyond, the cell types of interest. To enable more cell-type specific targeting, GECIs can be brought under the, control of the inte...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.10.006
更新日期：2012-01-01 00:00:00
abstract：:We analyzed spatio-temporal characteristics of Ca2+ transients in the cytosol and the nucleus of cultured neonatal rat heart cells using confocal imaging with Indo-1 and Fluo-3. In resting heart muscle cells, nuclear [Ca2+] was maintained lower than the cytosolic level. The rise in nuclear [Ca2+], during either E-C co...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90031-4
更新日期：1995-03-01 00:00:00
abstract：:Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2007.03.009
更新日期：2007-08-01 00:00:00
abstract：:The release of Ca2+ ions from the sarcoplasmic reticulum through ryanodine receptor calcium release channels represents the critical step linking electrical excitation to muscular contraction in the heart and skeletal muscle (excitation-contraction coupling). Two small Ca2+ binding proteins, S100A1 and calmodulin, hav...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.06.001
更新日期：2011-10-01 00:00:00
abstract：:The measurement of intracellular calcium ion concentrations [( Ca2+]i) in single living cells using quantitative fluorescence microscopy draws from a diverse set of disciplines, including cellular biology, optical physics, statistics and computer science. Over the last few years, we have devised and built a number of ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(90)90068-6
更新日期：1990-02-01 00:00:00
abstract：:The endoplasmic reticulum (ER) and mitochondria cannot be considered as static structures, as they intimately communicate, forming very dynamic platforms termed mitochondria-associated membranes (MAMs). In particular, the ER transmits proper Ca2+ signals to mitochondria, which decode them into specific inputs to regul...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.05.003
更新日期：2018-01-01 00:00:00
abstract：:Nanosecond-duration electric stimuli are distinguished by the ability to permeabilize intracellular membranes and recruit Ca2+ from intracellular stores. We quantified this effect in non-excitable cells (CHO) using ratiometric Ca2+ imaging with Fura-2. In a Ca(2+)-free medium, 10-, 60-, and 300-ns stimuli evoked Ca2+ ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.05.008
更新日期：2013-09-01 00:00:00
abstract：:Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca2+ mobilizing second messenger which triggers Ca2+ release in both sea urchin egg homogenates and in mammalian cells. The NAADP binding protein has not been identified and the regulation of NAADP mediated Ca2+ release remains controversial. To address...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.102060
更新日期：2019-11-01 00:00:00
abstract：:Photolysis of caged calcium (Nitr5, Calbiochem) can be used to study calcium dependent processes such as excitation-contraction coupling and muscular mechanics. Expensive high energy light sources are routinely used for UV light exposure, but this study describes an alternative low cost xenon flash unit constructed in...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90017-5
更新日期：1994-05-01 00:00:00
abstract：:The concentration of free Ca2+ in the cytoplasm and organelles of individual mouse pancreatic beta-cells was estimated with dual wavelength microfluorometry and the indicators Fura-2 and furaptra. Measuring the increase of cytoplasmic Ca2+ resulting from intracellular mobilization of the ion in ob/ob mouse beta-cells,...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0087
更新日期：2000-01-01 00:00:00
abstract：:The role of intracellular calcium stores in stimulus-secretion coupling in the pancreatic beta-cell is largely unknown. We report here that tetracaine stimulates insulin secretion from collagenase-isolated mouse islets of Langerhans in the absence of glucose or extracellular calcium. We also found that the anesthetic ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1998.0007
更新日期：1999-01-01 00:00:00
abstract：:A 106 kD protein was isolated from skeletal sarcoplasmic reticulum (SR) vesicles and shown to have the properties of SR Ca2+ release channels, including blockade by 5 nM ryanodine. In view of extensive reports that the ryanodine-receptor complex consists of four 565 kD junctional feet proteins (JFPs) and is the 'physi...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90074-3
更新日期：1992-11-01 00:00:00
abstract：:The spatiotemporal dynamics of elementary Ca(2+) release events, such as "blips" and "puffs" shapes the hierarchal Ca(2+) signaling in many cell types. Despite being the building blocks of Ca(2+) patterning, the mechanism responsible for the observed properties of puffs, especially their termination is incompletely un...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.04.018
更新日期：2012-08-01 00:00:00
abstract：:The Bcl-2 protein, best known for its ability to inhibit apoptosis, interacts with the inositol 1,4,5-trisphosphate receptor (IP(3)R) Ca(2+) channel to regulate IP(3)-mediated Ca(2+) release from the endoplasmic reticulum. This review summarizes the current state of knowledge regarding the interaction of Bcl-2, and al...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.05.011
更新日期：2011-09-01 00:00:00
abstract：:Hard end is a physiological disorder of pear fruit that is frequently observed in the 'Whangkeumbae' (Pyrus pyrifolia) variety, however, the mechanisms that are involved in its development are poorly understood. In this study, we explored the causes of hard end disorder in pear fruit in relation to calcium deficiency....
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2017.11.002
更新日期：2018-05-01 00:00:00
abstract：:K+-dependent Na+-Ca2+ exchangers (NCKXs) belong to Ca2+/cation antiporter gene superfamily. NCKX proteins play an important role in Ca2+ homeostasis and are bi-directional plasma membrane Ca2+-transporters which utilize the inward Na+ and outward K+ gradients to move Ca2+ ions into and out of the cytosol (4Na+:1Ca2+ +...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.06.002
更新日期：2018-09-01 00:00:00
