Abstract：
:Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brain. In this study, we use calcium sensitive dye imaging on macroscopic and microscopic scales to explore the spatiotemporal effects of INS on cortical calcium dynamics. The INS-evoked calcium signal that was observed exhibited a fast and slow component suggesting activation of multiple cellular mechanisms. The slow component of the evoked signal exhibited wave-like properties suggesting network activation, and was verified to originate from astrocytes through pharmacology and 2-photon imaging. We also provide evidence that the fast calcium signal may have been evoked through modulation of glutamate transients. This study demonstrates that pulsed infrared light can induce intracellular calcium modulations in both astrocytes and neurons, providing new insights into the mechanisms of action of INS in the brain.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Cayce JM,Bouchard MB,Chernov MM,Chen BR,Grosberg LE,Jansen ED,Hillman EM,Mahadevan-Jansen Adoi
10.1016/j.ceca.2014.01.004subject
Has Abstractpub_date
2014-04-01 00:00:00pages
183-90issue
4eissn
0143-4160issn
1532-1991pii
S0143-4160(14)00014-1journal_volume
55pub_type
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