Coupling acidic organelles with the ER through Ca²⁺ microdomains at membrane contact sites.

abstract：:We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-medi...

journal_title：Cell calcium

authors： Du T,Liang C,Li B,Hertz L,Peng L

更新日期：2014-03-01 00:00:00

abstract：:Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...

journal_title：Cell calcium

authors： Takahashi T

更新日期：2005-05-01 00:00:00

abstract：:In the brain, classical (canonical) transient receptor potential (TRPC) channels are thought to be involved in different aspects of neuronal development. We investigated the developmental expression profile of TRPC channels in rat cerebellum during the first 6 weeks after birth. TRPC3 expression is significantly up-re...

journal_title：Cell calcium

authors： Huang WC,Young JS,Glitsch MD

更新日期：2007-07-01 00:00:00

abstract：:A procedure for calibration of fluorescence signals from adult rat heart cells loaded with the -AM ester of fura-2 is described. Calibration is complicated by dye compartmentation and potentially incomplete dye hydrolysis. These problems were overcome by subtracting from fluorescence transients the non-cytosolic (mito...

journal_title：Cell calcium

authors： Haworth RA,Redon D

更新日期：1998-10-01 00:00:00

abstract：:Pacemaker cells residing in the sinoatrial node generate the regular heartbeat. Ca2+ signaling controls the heartbeat rate-directly, through the effect on membrane molecules (NCX exchange, K+ channel), and indirectly, through activation of calmodulin-AC-cAMP-PKA signaling. Thus, the physiological role of signaling in ...

journal_title：Cell calcium

authors： Segal S,Kirschner Peretz N,Arbel-Ganon L,Liang J,Li L,Marbach D,Yang D,Wang SQ,Yaniv Y

更新日期：2019-03-01 00:00:00

abstract：:Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...

journal_title：Cell calcium

authors： Fliegert R,Hölzer HT,Guse AH

更新日期：2018-12-01 00:00:00

abstract：:We have constructed a stochastic model of the inositol 1,4,5-trisphosphate receptor-Ca2+ channel that is based on quantitative measurements of the channel's properties. It displays the observed dependence of the open probability of the channel with cytosolic [Ca2+] and [IP3] and gives values for the dwell times that a...

journal_title：Cell calcium

authors： Fraiman D,Dawson SP

更新日期：2004-05-01 00:00:00

abstract：:The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) mediates increases in the intracellular concentration of Ca(2+) ([Ca(2+)]i) during fertilization in mammalian eggs. The activity of IP3R1 is enhanced during oocyte maturation, and phosphorylations by M-phase kinases are thought to positively regulate the activit...

journal_title：Cell calcium

authors： Zhang N,Yoon SY,Parys JB,Fissore RA

更新日期：2015-11-01 00:00:00

abstract：:In the complex signalling interplay that allows extracellular signals to be decoded into activation of apoptotic cell death, Ca(2+) plays a significant role. This is supported not only by evidence linking alterations in Ca(2+) homeostasis to the triggering of apoptotic (and in some cases necrotic) cell death, but also...

journal_title：Cell calcium

authors： Ferrari D,Pinton P,Szabadkai G,Chami M,Campanella M,Pozzan T,Rizzuto R

更新日期：2002-11-01 00:00:00

abstract：:The actions of the novel calcium (Ca2+) channel antagonist mibefradil (Ro 40-5967), a selective T-type channel blocker in myocardium, were investigated in embryonic rat spinal motoneurones maintained in culture. Whole-cell currents were recorded with the patch-clamp technique. Motoneurones displayed transient, low-vol...

journal_title：Cell calcium

authors： Viana F,Van den Bosch L,Missiaen L,Vandenberghe W,Droogmans G,Nilius B,Robberecht W

更新日期：1997-10-01 00:00:00

abstract：:The A23187 induced calcium uptake in ATP depleted cells was determined at pH 6.9 in the presence of trifluoperazine (TFP, 0.30 mM), compound 48/80 (0.89 mg/ml), 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8, 2.13 mM) and verapamil (1.81 mM). Apart from verapamil the drugs all increased the maximum rate of ...

journal_title：Cell calcium

authors： Foder B,Skibsted U,Scharff O

更新日期：1984-10-01 00:00:00

abstract：:Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...

journal_title：Cell calcium

authors： Chang JP,Habibi HR,Yu Y,Moussavi M,Grey CL,Pemberton JG

更新日期：2012-03-01 00:00:00

abstract：:Rabbit gall-bladder epithelial cells were isolated by a combination of Ca2+ omission, enzymatic treatment, and mechanical detachment and had a viability of 96-98% and well preserved morphology. Measurements of cytosolic free Ca2+ concentration ([Ca2+]i) in these cells with the Ca2+-fluorescent indicator fura-2 demonst...

journal_title：Cell calcium

authors： Bouchelouche PN,Hainau B,Frederiksen O

更新日期：1989-01-01 00:00:00

abstract：:Near-membrane [Ca2+] may differ significantly from bulk cytosolic [Ca2+], particularly during rapid Ca2+ signalling events related to cardiac muscle excitation-contraction coupling. We used the lipophilic membrane-associated Ca2+ indicator Ca(2+)-Green C-18 (C-18) and laser-scanning confocal microscopy to detect extra...

journal_title：Cell calcium

authors： Blatter LA,Niggli E

更新日期：1998-05-01 00:00:00

abstract：:Both theoretical and experimental results are presented for in vivo calibration of the dissociation constant K(Ca)(d)of the calcium-sensitive fluorescent dye Rhod(2)in the perfused mouse heart, using manganese quenching of fluorescence transients. An analytical model is derived, based on the biochemical equilibrium of...

journal_title：Cell calcium

authors： Du C,MacGowan GA,Farkas DL,Koretsky AP

更新日期：2001-04-01 00:00:00

abstract：:Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane...

journal_title：Cell calcium

authors： Morgan BP,Luzio JP,Campbell AK

更新日期：1986-12-01 00:00:00

abstract：:Nanosecond-duration electric stimuli are distinguished by the ability to permeabilize intracellular membranes and recruit Ca2+ from intracellular stores. We quantified this effect in non-excitable cells (CHO) using ratiometric Ca2+ imaging with Fura-2. In a Ca(2+)-free medium, 10-, 60-, and 300-ns stimuli evoked Ca2+ ...

journal_title：Cell calcium

authors： Semenov I,Xiao S,Pakhomova ON,Pakhomov AG

更新日期：2013-09-01 00:00:00

abstract：:The sensitivity to Ca2+ of the Ca2+-dependent K+ channel can be increased by the artificial electron donor system ascorbate + phenazine-methosulphate in a variety of animal cells. In the human erythrocyte the shift from the 'low' to the 'high-affinity' state seems to depend on the reduction of a membrane component acc...

journal_title：Cell calcium

authors： García-Sancho J,Herreros B

更新日期：1983-12-01 00:00:00

abstract：:Vesl/Homer proteins physically link proteins that mediate cellular signaling [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851] and thereby influence cellular function [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962]. A previous study reported that Vesl-1L/Homer-1c ...

journal_title：Cell calcium

authors： Hwang SY,Wei J,Westhoff JH,Duncan RS,Ozawa F,Volpe P,Inokuchi K,Koulen P

更新日期：2003-08-01 00:00:00

abstract：:To determine intracellular Ca2+ concentrations more accurately, we examined Kd of Fura-2 for Ca2+ in conditions which were systemically changed. In a solution comprising of 150 mM KCI, 20 mM MOPS-KOH, pH 6.94, 60-100 microM EGTA and 1 microM Fura-2, Kd at 20 degrees C was 0.266 +/- 0.016 microM (mean +/- SEM) (21 dete...

journal_title：Cell calcium

authors： Uto A,Arai H,Ogawa Y

更新日期：1991-01-01 00:00:00

abstract：:Calcium entry from the extracellular space into cells is an important signaling mechanism in both physiological and pathophysiological functions. In non-excitable cells, store-operated calcium (SOC) entry represents a principal mode of calcium entry. Activation of SOC entry in pulmonary artery endothelial cells leads ...

journal_title：Cell calcium

authors： Kadeba PI,Vasauskas AA,Chen H,Wu S,Scammell JG,Cioffi DL

更新日期：2013-04-01 00:00:00

abstract：:The sphingosine derivatives sphingosylphosphorylcholine (SPC) and sphingosine-1-phosphate (S1P) caused a similar elevation of the intracellular Ca2+ concentration ([Ca2+]i) in an immortalized airway epithelial cell line (CFNP9o-) incubated in Ca(2+)-free medium. The maximal effect was obtained with 2 microM SPC and 0....

journal_title：Cell calcium

authors： Orlati S,Porcelli AM,Hrelia S,Rugolo M

更新日期：1998-06-01 00:00:00

abstract：:The regulation of the Ca2+ -induced Ca2+ release (CICR) from intracellular stores is a critical step in the cardiac cycle. The inherent positive feedback of CICR should make it a self-regenerating process. It is accepted that CICR must be governed by some negative control, but its nature is still debated. We explore h...

journal_title：Cell calcium

authors： Zoghbi ME,Copello JA,Villalba-Galea CA,Vélez P,Diaz Sylvester PL,Bolaños P,Marcano A,Fill M,Escobar AL

更新日期：2004-08-01 00:00:00

abstract：:Mechanically induced channel activities and increase of intracellular calcium ([Ca2+]i) in normal and cancerous murine mammary cells (MMT 060562) were investigated using the patch clamp technique and Fura-2 fluorescence. Both cell types showed similar properties. Upon mechanical stimulation, activation of the Ca(2+)-d...

journal_title：Cell calcium

authors： Enomoto K,Furuya K,Yamagishi S,Maeno T

更新日期：1992-08-01 00:00:00

abstract：:We have investigated the effects of glucose on cytosolic free calcium concentration in the insulin-secreting cell line HIT-T15. Addition of glucose (10 mM) caused a 20-75% increase in cytosolic [Ca2+] within 5 minutes compared to controls in the absence of glucose. A maximal increase in cytosolic [Ca2+] was obtained w...

journal_title：Cell calcium

authors： Meats JE,Best L,Lynch AM,Tomlinson S

更新日期：1989-11-01 00:00:00

abstract：:The glucoregulatory hormones insulin and glucagon are released from the β- and α-cells of the pancreatic islets. In both cell types, secretion is secondary to firing of action potentials, Ca(2+)-influx via voltage-gated Ca(2+)-channels, elevation of [Ca(2+)](i) and initiation of Ca(2+)-dependent exocytosis. Here we di...

journal_title：Cell calcium

authors： Rorsman P,Braun M,Zhang Q

更新日期：2012-03-01 00:00:00

abstract：:TRPV6 belongs to the vanilloid family of the transient receptor potential channel (TRP) superfamily. This calcium-selective channel is highly expressed in the duodenum and the placenta, being responsible for calcium absorption in the body and fetus. Previous observations have suggested that TRPV6 is not only permeable...

journal_title：Cell calcium

authors： Kovacs G,Danko T,Bergeron MJ,Balazs B,Suzuki Y,Zsembery A,Hediger MA

更新日期：2011-01-01 00:00:00

abstract：:Recent developments in microscopy and fluorescent indicators now make it possible to monitor the activity and localization of membrane ion channels by imaging Ca(2+) flux through individual channels. Such optical approaches have advantages over electrophysiological single-channel techniques in that they are less invas...

journal_title：Cell calcium

authors： Shuai J,Parker I

更新日期：2005-04-01 00:00:00

abstract：:This investigation concentrates on the change in Ca(2+) concentration ([Ca(2+)]) caused by ryanodine in U373 MG cells. This cell type from a human astrocytoma is a unique cellular model because it only expresses the type 3 ryanodine receptor (RyR3), which is generally the least abundant isoform. In the presence of phy...

journal_title：Cell calcium

authors： Galiano M,Gasparre G,Lippe C,Cassano G

更新日期：2004-11-01 00:00:00

abstract：:This work shows, for the first time, a properly metabolically regulated squid nerve Na(+)/Ca(2+) exchanger (NCXSQ1) heterologous expressed in Saccharomyces cerevisiae. The exchanger was fused to the enhanced green fluorescence protein (eGFP) on its C-terminus and had two tags, a Strep-tag II and 6 histidines, added to...

journal_title：Cell calcium

authors： Raimunda D,Bollo M,Beaugé L,Berberián G

更新日期：2009-05-01 00:00:00