Coupling acidic organelles with the ER through Ca²⁺ microdomains at membrane contact sites.

abstract：:Studies of Ca2+ transport pathways in exocrine gland cells have been useful, chiefly because of the polarized nature of the secretory epithelial cells. In pancreatic acinar cells, for example, Ca2+ reloading of empty intracellular stores can occur solely via Ca2+ entry through the basal part of the plasma membrane. On...

journal_title：Cell calcium

authors： Petersen OH

更新日期：2003-05-01 00:00:00

abstract：:Odontoblasts are involved in the transduction of stimuli applied to exposed dentin. Although expression of thermo/mechano/osmo-sensitive transient receptor potential (TRP) channels has been demonstrated, the properties of TRP vanilloid 1 (TRPV1)-mediated signaling remain to be clarified. We investigated physiological ...

journal_title：Cell calcium

authors： Tsumura M,Sobhan U,Muramatsu T,Sato M,Ichikawa H,Sahara Y,Tazaki M,Shibukawa Y

更新日期：2012-08-01 00:00:00

abstract：:The formation of a heterotetrameric complex between annexin 2 (anx 2) and S100A10 plays an important role in regulating the cellular distribution and biochemical properties of anx 2. A major distinction between the anx 2-S100A10 complex and other annexin-S100 complexes is that S100A10 binding to anx 2 occurs independe...

journal_title：Cell calcium

authors： Borthwick LA,Neal A,Hobson L,Gerke V,Robson L,Muimo R

更新日期：2008-08-01 00:00:00

abstract：:Calcium regulates the proliferation and differentiation of keratinocytes both in vivo and in vitro. Elevated extracellular Ca2+ concentration ([Ca2+]o) raises the intracellular free calcium ([Ca2+]i) and activates differentiation-related genes. Cells lacking the calcium-sensing receptor (CaR) fail to respond to [Ca2+]...

journal_title：Cell calcium

authors： Tu CL,Oda Y,Komuves L,Bikle DD

更新日期：2004-03-01 00:00:00

abstract：:Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicabilit...

journal_title：Cell calcium

authors： Razani-Boroujerdi S,Partridge LD,Sopori ML

更新日期：1994-12-01 00:00:00

abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...

journal_title：Cell calcium

authors： Barclay JW,Morgan A,Burgoyne RD

更新日期：2005-09-01 00:00:00

abstract：:The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...

journal_title：Cell calcium

authors： De Marchi E,Bonora M,Giorgi C,Pinton P

更新日期：2014-07-01 00:00:00

abstract：:As pivotal players in cellular metabolism, mitochondria have a double-faceted role in the final decision of cell fate. This is true for all cell types, but it is even more important and intriguing in the cancer setting. Mitochondria regulate cell fate in many diverse ways: through metabolism, by producing ATP and othe...

journal_title：Cell calcium

authors： Genovese I,Vezzani B,Danese A,Modesti L,Vitto VAM,Corazzi V,Pelucchi S,Pinton P,Giorgi C

更新日期：2020-12-01 00:00:00

abstract：:Receptor-activated cytoplasmic calcium (Ca2+) oscillations have been investigated in single pancreatic acinar cells by microfluorimetry (Fura-2 as indicator). At submaximal concentrations of the agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8), both give rise to oscillatory changes in the cytosolic...

journal_title：Cell calcium

authors： Yule DI,Lawrie AM,Gallacher DV

更新日期：1991-02-01 00:00:00

abstract：:We report here the first local and global Ca(2+) measurements made from in situ terminal arterioles. The advantages of the method are that there is minimal disturbance to the vessels, which retain their relationship to the tissue they are supplying (rat ureter) and the small size of vessel that can be studied. Good lo...

journal_title：Cell calcium

authors： Burdyga T,Shmygol A,Eisner DA,Wray S

更新日期：2003-07-01 00:00:00

abstract：:There is a close association of peripheral sarcoplasmic reticulum (SR), containing IP3 receptors, and regions of the plasma membrane enriched in the Na+/Ca2+ exchanger in smooth muscle. We have tested the possibility in rat uterine smooth muscle that Ca2+ released from the SR is preferentially removed from the cytosol...

journal_title：Cell calcium

authors： Taggart MJ,Wray S

更新日期：1997-11-01 00:00:00

abstract：:Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...

journal_title：Cell calcium

authors： Chang JP,Habibi HR,Yu Y,Moussavi M,Grey CL,Pemberton JG

更新日期：2012-03-01 00:00:00

abstract：:An enzymatically active Ca2+-stimulated ATPase has been isolated from the sarcolemmal sheets of human smooth muscle (myometrium). Ca2+-ATPase activity was quantitated in an assay medium which simulated the characteristic free ionic concentrations of the cytosol. New computer programs for calculating the composition of...

journal_title：Cell calcium

authors： Popescu LM,Ignat P

更新日期：1983-10-01 00:00:00

abstract：:Alternative splicing of the sarco/endoplasmic reticulum (SERCA2) Ca2+ pump transcript generates the two isoforms: SERCA2a in left ventricular myocytes (LVM) and SERCA2b in most tissues. Nuclear protein extracts from left ventricular myocytes can cause a decay of the 3'-region of the SERCA2a. To determine if all the do...

journal_title：Cell calcium

authors： Misquitta CM,Ghosh P,Mwanjewe J,Grover AK

更新日期：2005-01-01 00:00:00

abstract：:Cellular calcium signaling events are transient. Hence they are observed in real time using fluorescence imaging or electrophysiological methods that require sophisticated instrumentation and specialized skills. For high throughput assays simple and inexpensive techniques are desirable. Many calcium channels that serv...

journal_title：Cell calcium

authors： Chandrika AR,Steephan M,Raveendran Nair R,Sudarsana Devi SP,Kumar M,Paul S,Madhavan M,Omkumar RV

更新日期：2018-09-01 00:00:00

abstract：:Agonist induced increases in intracellular free calcium, [Ca2+]i, were measured in single Fura-2 loaded bovine aortic endothelial (BAE) cells by dual wavelength microspectrofluorimetry. Low doses of ATP (less than 10 microM) induced complex changes in [Ca2+]i. These changes usually consisted of a large initial transie...

journal_title：Cell calcium

authors： Lynch M,Gillespie JI,Greenwell JR,Johnson C

更新日期：1992-04-01 00:00:00

abstract：:Previously, we have used the classical approach to examine intracellular calcium stores in human foreskin fibroblasts (HSWP) cells. In this classical protocol cells are first permeabilized and then allowed to fill their Ca2+ reservoirs with 45Ca2+ in the presence of ATP. In this paper we present an alternative method ...

journal_title：Cell calcium

authors： Baumgarten LB,Lee HC,Villereal ML

更新日期：1995-01-01 00:00:00

abstract：:There is growing evidence indicating that the pore structure of voltage-gated ion channels (VGICs) influences gating besides their conductance. Regarding low voltage-activated (LVA) Ca2+ channels, it has been demonstrated that substitutions of the pore aspartate (D) by a glutamate (D-to-E substitution) in domains III ...

journal_title：Cell calcium

authors： Garza-López E,Aldana A,Darszon A,Nishigaki T,López-González I

更新日期：2020-07-01 00:00:00

abstract：:The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...

journal_title：Cell calcium

authors： Tiffert T,Lew VL

更新日期：2001-11-01 00:00:00

abstract：:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel which plays a major role in Ca2+ signalling. Three isoforms of IP3R have been identified (IP3R-1, IP3R-2 and IP3R-3) and most cell types express different proportions of each isoform. The differences between the p...

journal_title：Cell calcium

authors： Caron AZ,Chaloux B,Arguin G,Guillemette G

更新日期：2007-09-01 00:00:00

abstract：:The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...

journal_title：Cell calcium

authors： Bihler I,Charles P,Sawh PC

更新日期：1982-08-01 00:00:00

abstract：:The Solute Carrier Family 24 (SLC24) belongs to the CaCA super family of Ca(2+)/cation antiporters and codes for five different K(+)- dependent Na(+)- Ca(2+) exchangers (NCKX1-5). NCKX proteins play a critical role in Ca(2+) homeostasis in a wide variety of biological processes such as vision, olfaction, enamel format...

journal_title：Cell calcium

authors： Jalloul AH,Szerencsei RT,Schnetkamp PP

更新日期：2016-01-01 00:00:00

abstract：:Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...

journal_title：Cell calcium

authors： Stalmans P,Himpens B

更新日期：1999-06-01 00:00:00

abstract：:Burst firing of the thalamic neurons is driven by the low threshold Ca2+ spike generated by Ca2+ influx through T-type Ca2+ channels when these channels are activated by membrane hyperpolarization due to inhibitory inputs. The major inhibitory inputs to the thalamocortical (TC) neurons are from the GABAergic neurons i...

journal_title：Cell calcium

authors： Shin HS

更新日期：2006-08-01 00:00:00

abstract：:TRPM family (Transient receptor potential channels, M for melastatin) is a group of intrinsic plasma membrane ion channels which are widely expressed throughout human body. It has been identified as a potent entry point of working desperate diseases out in a new way with newfangled ideas and safer technological means....

journal_title：Cell calcium

authors： Chen Y,Zhang X,Yang T,Bi R,Huang Z,Ding H,Li J,Zhang J

更新日期：2019-05-01 00:00:00

abstract：:Extracellular nucleotides are potent Ca2+ mobilizing agents. A variety of receptors for extracellular ATP are recognised. Some are involved in fast neuronal transmission and operate as ligand-gated ion channels. Others are involved in the paracrine or autocrine modulation of cell function. Many receptors of this type ...

journal_title：Cell calcium

authors： Conigrave AD,Jiang L

更新日期：1995-02-01 00:00:00

abstract：:We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-medi...

journal_title：Cell calcium

authors： Du T,Liang C,Li B,Hertz L,Peng L

更新日期：2014-03-01 00:00:00

abstract：:Using single-cell ratio imaging of Fura-2-loaded neutrophils, we demonstrate that the heterogeneity and asynchrony of the oxidase response originates from variability in the timing and magnitude of the cytosolic free Ca2+ signal. The Ca2+ signals from individual cells could be classified into four types: (a) type 1, a...

journal_title：Cell calcium

authors： Hallett MB,Davies EV,Campbell AK

更新日期：1990-11-01 00:00:00

abstract：:For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed he...

journal_title：Cell calcium

authors： Grover AK

更新日期：1985-06-01 00:00:00

abstract：:The mammalian transient receptor potential (TRP) proteins consist of a superfamily of Ca2+-permeant non-selective cation channels with structural similarities to Drosophila TRP. The TRP superfamily can be divided into three major families, among them the "canonical TRP" family (TRPC). The seven protein products of the...

journal_title：Cell calcium

authors： Trebak M,Vazquez G,Bird GS,Putney JW Jr

更新日期：2003-05-01 00:00:00