The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia.


:The formation of a heterotetrameric complex between annexin 2 (anx 2) and S100A10 plays an important role in regulating the cellular distribution and biochemical properties of anx 2. A major distinction between the anx 2-S100A10 complex and other annexin-S100 complexes is that S100A10 binding to anx 2 occurs independently of calcium. Here we describe a cyclic 3',5'-adenosine monophosphate (cAMP) and cAMP-dependent protein kinase (PKA, EC mechanism regulating anx 2-S100A10 complex formation and its interaction with the transient receptor potential vanilloid type 6 channel (TRPV6) in airway and gut epithelia. In both 16HBE14o- and Caco-2 cells, forskolin (FSK) stimulated increased anx 2-S100A10 complex formation, which was attenuated by either PKA inhibitors or calcineurin A (CnA) inhibitors. The anx 2-S100A10 complex association with TRPV6 was dependent on FSK-induced CnA-dependent dephosphorylation of anx 2. Analysis of the significance of the cAMP/PKA/CnA pathway on calcium influx showed that both PKA and CnA inhibitors attenuated Ca(45) uptake in Caco-2, but not 16HBE14o-, cells. Thus, the cAMP/PKA/CnA-induced anx 2-S100A10/TRPV6 complex may require additional factors for calcium influx or play a role independent of calcium influx in airway epithelia. In conclusion, our data demonstrates that cAMP/PKA/CnA signalling is important for anx 2-S100A10 complex formation and interaction with target molecules in both absorptive and secretory epithelia.


Cell Calcium


Cell calcium


Borthwick LA,Neal A,Hobson L,Gerke V,Robson L,Muimo R




Has Abstract


2008-08-01 00:00:00














  • Purinoceptor P2U identification and function in human intrahepatic biliary epithelial cell lines.

    abstract::The mechanisms that regulate ion and fluid transport by the human intrahepatic bile duct have not been well defined. Human intrahepatic biliary cell lines that we have developed were used to identify and characterize purinoceptors based on increases in intracellular calcium in response to ATP and other nucleotides. In...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Wolkoff LI,Perrone RD,Grubman SA,Lee DW,Soltoff SP,Rogers LC,Beinborn M,Fang SL,Cheng SH,Jefferson DM

    更新日期:1995-05-01 00:00:00

  • Inhibition of calmodulin-dependent and independent cardiac sarcoplasmic reticulum activities by R24571.

    abstract::R24571 a derivative of the antimycotic miconazole, appears to be 5 to 8 times more potent than trifluoperazine in its ability to inhibit the calmodulin-dependent phosphorylation of cardiac sarcoplasmic reticulum vesicles. The cAMP-dependent protein kinase mediated phosphorylation of cardiac sarcoplasmic reticulum was ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Louis CF,Turnquist J,Jarvis B

    更新日期:1983-04-01 00:00:00

  • Ca2+ imaging in single living cells: theoretical and practical issues.

    abstract::The measurement of intracellular calcium ion concentrations [( Ca2+]i) in single living cells using quantitative fluorescence microscopy draws from a diverse set of disciplines, including cellular biology, optical physics, statistics and computer science. Over the last few years, we have devised and built a number of ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Moore ED,Becker PL,Fogarty KE,Williams DA,Fay FS

    更新日期:1990-02-01 00:00:00

  • Inhibition of ADP-induced intracellular Ca2+ responses and platelet aggregation by the P2Y12 receptor antagonists AR-C69931MX and clopidogrel is enhanced by prostaglandin E1.

    abstract::P2Y(12) antagonists such as clopidogrel and AR-C69931MX inhibit aggregation by antagonizing the effects of ADP at P2Y(12) receptors on platelets. Agents such as PGE(1) also inhibit aggregation by stimulating adenylate cyclase to produce cAMP, which interferes with Ca(2+) mobilization within the cell. Since one facet o...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Fox SC,Behan MW,Heptinstall S

    更新日期:2004-01-01 00:00:00

  • [Na(+)] in the subsarcolemmal 'fuzzy' space and modulation of [Ca(2+)](i) and contraction in cardiac myocytes.

    abstract::The strength of the heart beat depends on the amplitude and time course of the transient increase in [Ca(2+)] in the myocytes with each cycle. [Na(+)](i) modulates cardiac contraction through its effect on the Ca(2+) flux through the Na/Ca exchanger. Cardiac excitation-contraction coupling has been postulated to occur...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Verdonck F,Mubagwa K,Sipido KR

    更新日期:2004-06-01 00:00:00

  • Estradiol modulation of PMA--and ionomycin-stimulated LH secretion from pituitaries of castrated rats.

    abstract::Quartered pituitaries from castrated (72 h) +/- estradiol (E2)-treated (24 h) rats were used in a perifusion system to investigate estradiol modulation of ionomycin and ionomycin + PMA stimulated LH secretion. Estradiol enhanced the LH responses to GnRH (1 nM) and ionomycin (10 microM), and was necessary for the manif...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Fahmy NW,Bourne GA

    更新日期:1993-01-01 00:00:00

  • Comprehensive structural, interaction and expression analysis of CBL and CIPK complement during abiotic stresses and development in rice.

    abstract::Calcium ion is involved in diverse physiological and developmental pathways. One of the important roles of calcium is a signaling messenger, which regulates signal transduction in plants. CBL (calcineurin B-like protein) is one of the calcium sensors that specifically interact with a family of serine-threonine protein...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Kanwar P,Sanyal SK,Tokas I,Yadav AK,Pandey A,Kapoor S,Pandey GK

    更新日期:2014-08-01 00:00:00

  • Residues important for Ca2+ ion transport in the neuronal K+-dependent Na+-Ca2+ exchanger (NCKX2).

    abstract::K+-dependent Na+-Ca2+ exchangers (NCKXs) belong to Ca2+/cation antiporter gene superfamily. NCKX proteins play an important role in Ca2+ homeostasis and are bi-directional plasma membrane Ca2+-transporters which utilize the inward Na+ and outward K+ gradients to move Ca2+ ions into and out of the cytosol (4Na+:1Ca2+ +...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Jalloul AH,Liu G,Szerencsei RT,Schnetkamp PPM

    更新日期:2018-09-01 00:00:00

  • Parvalbumin as a metal-dependent antioxidant.

    abstract::Parvalbumin (PA) is a Ca(2+)-binding protein of vertebrates massively expressed in tissues with high oxygen uptake and respectively elevated level of reactive oxygen species (ROS). To characterize antioxidant properties of PA, antioxidant capacity (AOC) of intact rat α-PA has been explored. ORAC, TEAC and hydrogen per...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Permyakov SE,Kazakov AS,Avkhacheva NV,Permyakov EA

    更新日期:2014-05-01 00:00:00

  • A comparative assessment of fluo Ca2+ indicators in rat ventricular myocytes.

    abstract::The fluo family of indicators is frequently used in studying Ca(2+) physiology; however, choosing which fluo indicator to use is not obvious. Indicator properties are typically determined in well-defined aqueous solutions. Inside cells, however, the properties can change markedly. We have characterized each of three f...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Hagen BM,Boyman L,Kao JP,Lederer WJ

    更新日期:2012-08-01 00:00:00

  • Capacitative calcium entry in the nervous system.

    abstract::Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Putney JW Jr

    更新日期:2003-10-01 00:00:00

  • Chronic fluoxetine administration increases expression of the L-channel gene Cav1.2 in astrocytes from the brain of treated mice and in culture and augments K(+)-induced increase in [Ca(2+)]i.

    abstract::We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-medi...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Du T,Liang C,Li B,Hertz L,Peng L

    更新日期:2014-03-01 00:00:00

  • Glucose induces temperature-dependent oscillations of cytoplasmic Ca2+ in single pancreatic beta-cells related to their electrical activity.

    abstract::Glucose induces large amplitude oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i) in pancreatic beta-cells. The effects of temperature on these oscillations were examined by monitoring [Ca2+]i continuously in single beta-cells from ob/ob-mice using dual wavelength microfluorometry. The oscillations of [Ca2+...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Hellman B,Gylfe E,Grapengiesser E,Panten U,Schwanstecher C,Heipel C

    更新日期:1990-06-01 00:00:00

  • ET-1-induced growth promoting responses involving ERK1/2 and PKB signaling and Egr-1 expression are mediated by Ca2+/CaM-dependent protein kinase-II in vascular smooth muscle cells.

    abstract::Endothelin-1 (ET-1), a potent vasoactive peptide with a pathogenic role in vascular diseases, has been shown to induce the activation of ERK1/2, PKB and the expression of a transcriptional regulator, the early growth response 1 (Egr-1), key mediators of hypertrophic and proliferative responses in vascular smooth muscl...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Bouallegue A,Simo Cheyou ER,Anand-Srivastava MB,Srivastava AK

    更新日期:2013-12-01 00:00:00

  • Glucose increases cytosolic calcium concentration and inositol lipid metabolism in HIT-T15 cells.

    abstract::We have investigated the effects of glucose on cytosolic free calcium concentration in the insulin-secreting cell line HIT-T15. Addition of glucose (10 mM) caused a 20-75% increase in cytosolic [Ca2+] within 5 minutes compared to controls in the absence of glucose. A maximal increase in cytosolic [Ca2+] was obtained w...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Meats JE,Best L,Lynch AM,Tomlinson S

    更新日期:1989-11-01 00:00:00

  • A role for the intracellular protease calpain in the activation of store-operated calcium entry in human platelets.

    abstract::Here, we report a novel role for the cysteine protease calpain in store-operated calcium entry. Several structurally and mechanistically unrelated inhibitors of calpain inhibited Ca2+ entry activated in human platelets by thapsigargin-evoked Ca2+ store depletion or the physiological agonist thrombin, whereas inhibitor...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Harper AG,Sage SO

    更新日期:2007-02-01 00:00:00

  • Ca(2+) signaling, genes and the cell cycle.

    abstract::Changes in the concentration and spatial distribution of Ca(2+) ions in the cytoplasm constitute a ubiquitous intracellular signaling module in cellular physiology. With the advent of Ca(2+) dyes that allow direct visualization of Ca(2+) transients, combined with powerful experimental tools such as electrophysiologica...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Machaca K

    更新日期:2010-11-01 00:00:00

  • Mobilization of Ca2+ stores in individual pancreatic beta-cells permeabilized or not with digitonin or alpha-toxin.

    abstract::The concentration of free Ca2+ in the cytoplasm and organelles of individual mouse pancreatic beta-cells was estimated with dual wavelength microfluorometry and the indicators Fura-2 and furaptra. Measuring the increase of cytoplasmic Ca2+ resulting from intracellular mobilization of the ion in ob/ob mouse beta-cells,...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Tengholm A,Hellman B,Gylfe E

    更新日期:2000-01-01 00:00:00

  • The aging mouse brain: cognition, connectivity and calcium.

    abstract::Aging is a complex process that differentially impacts multiple cognitive, sensory, neuronal and molecular processes. Technological innovations now allow for parallel investigation of neuronal circuit function, structure and molecular composition in the brain of awake behaving adult mice. Thus, mice have become a crit...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Radulescu CI,Cerar V,Haslehurst P,Kopanitsa M,Barnes SJ

    更新日期:2021-01-23 00:00:00

  • Sensing of redox status by TRP channels.

    abstract::Cellular redox status is maintained by the balance between series of antioxidant systems and production of reactive oxygen/nitrogenous species. Cells utilize this redox balance to mediate diverse physiological functions. Transient receptor potential (TRP) channels are non-selective cation channels that act as biosenso...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Ogawa N,Kurokawa T,Mori Y

    更新日期:2016-08-01 00:00:00

  • Ca2+ signaling and exocytosis in pituitary corticotropes.

    abstract::The secretion of adrenocorticotrophin (ACTH) from corticotropes is a key component in the endocrine response to stress. The resting potential of corticotropes is set by the basal activities of TWIK-related K(+) (TREK)-1 channel. Corticotrophin-releasing hormone (CRH), the major ACTH secretagogue, closes the background...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Tse A,Lee AK,Tse FW

    更新日期:2012-03-01 00:00:00

  • Changes in functional properties of the caffeine-sensitive Ca2+ store during differentiation of human SH-SY5Y neuroblastoma cells.

    abstract::We have used single cell fluorescence imaging techniques to examine how functional properties of the caffeine-sensitive Ca(2+) store change during differentiation of a sub-population of caffeine-sensitive SH-SY5Y cells. Application of caffeine (30 mM) 1-10.5 min after a 'priming' depolarisation pulse of 55 mM K(+) rev...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Riddoch FC,Brown AM,Rowbotham SE,Redfern CP,Cheek TR

    更新日期:2007-03-01 00:00:00

  • Intact pancreatic islets and dispersed beta-cells both generate intracellular calcium oscillations but differ in their responsiveness to glucose.

    abstract::Pancreatic islets produce pulses of insulin and other hormones that maintain normal glucose homeostasis. These micro-organs possess exquisite glucose-sensing capabilities, allowing for precise changes in pulsatile insulin secretion in response to small changes in glucose. When communication among these cells is disrup...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Scarl RT,Corbin KL,Vann NW,Smith HM,Satin LS,Sherman A,Nunemaker CS

    更新日期:2019-11-01 00:00:00

  • X-ray microprobe analysis of epithelial calcium transport.

    abstract::The sternal epithelium of Porcellio scaber was used as a novel model to study the subcellular elemental distribution in control and Ca(2+)-transporting stages in situ. The anterior sternal epithelium (ASE) is specialized for transport of cuticular Ca to sternal CaCO(3) deposits during premolt, and from these deposits ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Ziegler A

    更新日期:2002-06-01 00:00:00

  • Regulation of calcium in pancreatic α- and β-cells in health and disease.

    abstract::The glucoregulatory hormones insulin and glucagon are released from the β- and α-cells of the pancreatic islets. In both cell types, secretion is secondary to firing of action potentials, Ca(2+)-influx via voltage-gated Ca(2+)-channels, elevation of [Ca(2+)](i) and initiation of Ca(2+)-dependent exocytosis. Here we di...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Rorsman P,Braun M,Zhang Q

    更新日期:2012-03-01 00:00:00

  • S-100b protein regulates aggregation and fusion of cardiolipin vesicles.

    abstract::We have recently shown that S-100b protein interacts with the polar surface of cardiolipin vesicles [6]. This interaction produces changes in the secondary structure of S-100b as well as changes in the structural organization of cardiolipin vesicles. We report here on the effects of S-100b on cardiolipin vesicles as i...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Zolese G,Curatola G,Amati S,Giambanco I,Donato R

    更新日期:1990-01-01 00:00:00

  • Localized calcium signalling and neuronal integration in cerebellar Purkinje neurones.

    abstract::The use of high resolution imaging techniques has revealed new forms of dendritic signal integration in neurones. In contrast to electrical signals that have a more widespread influence on the cell, brief Ca2+ transients resulting from synaptic activation are often restricted to a small part of the dendritic tree. In ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Eilers J,Plant T,Konnerth A

    更新日期:1996-08-01 00:00:00

  • The binding of HIV-1 gp41 membrane proximal domain to its mucosal receptor, galactosyl ceramide, is structure-dependent.

    abstract::The peptide of HIV-1 envelope gp41 (a.a 628-683), referred to herein as P5, contains P1, a conserved galactose-specific lectin domain for binding the mucosal HIV-1-receptor, galactosyl ceramide (GalCer), as shown earlier, and a potential calcium-binding site (a.a 628-648). P1 contains contiguous epitopes recognized by...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Yu H,Alfsen A,Tudor D,Bomsel M

    更新日期:2008-01-01 00:00:00

  • The still uncertain identity of the channel-forming unit(s) of the mitochondrial permeability transition pore.

    abstract::Mitochondria from different organisms can undergo a sudden process of inner membrane unselective leakiness to molecules known as the mitochondrial permeability transition (MPT). This process has been studied for nearly four decades and several proteins have been claimed to constitute, or at least regulate the usually ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Baines CP,Gutiérrez-Aguilar M

    更新日期:2018-07-01 00:00:00

  • Role of STIM and Orai proteins in the store-operated calcium signaling pathway.

    abstract::Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Hewavitharana T,Deng X,Soboloff J,Gill DL

    更新日期:2007-08-01 00:00:00