Abstract:
:The formation of a heterotetrameric complex between annexin 2 (anx 2) and S100A10 plays an important role in regulating the cellular distribution and biochemical properties of anx 2. A major distinction between the anx 2-S100A10 complex and other annexin-S100 complexes is that S100A10 binding to anx 2 occurs independently of calcium. Here we describe a cyclic 3',5'-adenosine monophosphate (cAMP) and cAMP-dependent protein kinase (PKA, EC 2.7.1.37)-dependent mechanism regulating anx 2-S100A10 complex formation and its interaction with the transient receptor potential vanilloid type 6 channel (TRPV6) in airway and gut epithelia. In both 16HBE14o- and Caco-2 cells, forskolin (FSK) stimulated increased anx 2-S100A10 complex formation, which was attenuated by either PKA inhibitors or calcineurin A (CnA) inhibitors. The anx 2-S100A10 complex association with TRPV6 was dependent on FSK-induced CnA-dependent dephosphorylation of anx 2. Analysis of the significance of the cAMP/PKA/CnA pathway on calcium influx showed that both PKA and CnA inhibitors attenuated Ca(45) uptake in Caco-2, but not 16HBE14o-, cells. Thus, the cAMP/PKA/CnA-induced anx 2-S100A10/TRPV6 complex may require additional factors for calcium influx or play a role independent of calcium influx in airway epithelia. In conclusion, our data demonstrates that cAMP/PKA/CnA signalling is important for anx 2-S100A10 complex formation and interaction with target molecules in both absorptive and secretory epithelia.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Borthwick LA,Neal A,Hobson L,Gerke V,Robson L,Muimo Rdoi
10.1016/j.ceca.2007.11.001subject
Has Abstractpub_date
2008-08-01 00:00:00pages
147-57issue
2eissn
0143-4160issn
1532-1991pii
S0143-4160(07)00185-6journal_volume
44pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::We have previously demonstrated a role for the reorganization of the actin cytoskeleton in store-operated calcium entry (SOCE) in human platelets and interpreted this as evidence for a de novo conformational coupling step in SOCE activation involving the type II IP(3) receptor and the platelet hTRPC1-containing store-...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.02.004
更新日期:2007-12-01 00:00:00
abstract::In previous studies we have characterised various properties of capacitative Ca2+ entry (CCE) in different epithelia. After Ca2+ store depletion with PLC/InsP3-coupled agonists or by inhibition of store Ca2+ uptake, with for example thapsigargin, Ca2+ influx is activated. This leads to a sustained cellular response (e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90112-3
更新日期:1997-08-01 00:00:00
abstract::Store operated Ca(2+) entry (SOCE) replenishes intracellular Ca(2+) stores and activates a number of intracellular signalling pathways. Whilst several molecular components forming store operated Ca(2+) channels (SOCC) have been identified, their modulation in neurons remains poorly understood. Here, we extend on our p...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2012.11.004
更新日期:2013-02-01 00:00:00
abstract::Calcium-activated potassium channels are important in a variety of smooth muscles, contributing to excitability and contractility. In the myometrium previous work has focussed on the large conductance channels (BK), and the role of small conductance channels (SK) has received scant attention, despite the finding that ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.11.004
更新日期:2010-01-01 00:00:00
abstract::Animals have evolved sophisticated physiological systems for sensing ambient temperature since changes in environmental temperatures affect various biological processes. Thermosensitive transient receptor potential (thermoTRP) channels serve as thermal sensors in diverse animal species. They are multimodal receptors t...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2014.12.001
更新日期:2015-03-01 00:00:00
abstract::TRPV6 belongs to the vanilloid family of the transient receptor potential channel (TRP) superfamily. This calcium-selective channel is highly expressed in the duodenum and the placenta, being responsible for calcium absorption in the body and fetus. Previous observations have suggested that TRPV6 is not only permeable...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.11.007
更新日期:2011-01-01 00:00:00
abstract::Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...
journal_title:Cell calcium
pub_type: 评论,杂志文章
doi:10.1016/j.ceca.2018.11.001
更新日期:2018-12-01 00:00:00
abstract::Antisense oligodeoxynucleotides (AS-ODNs) were used in combination with transient functional expression of the cardiac Na(+)-Ca2+ exchanger (NCX1) to correlate suppression of the Na(+)-Ca2+ exchange function with down-regulation of NCX1 protein expression. In a de-novo expression system (Sf9 cells), a decrease in both...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.10.006
更新日期:2005-03-01 00:00:00
abstract::NAADP (nicotinic acid adenine dinucleotide phosphate) is a recently discovered second messenger, and as such, we have much yet to learn about its functions in health and disease. A bottleneck in this basic research is due to NAADP, like all second messengers, being charged to prevent it from leaking out of cells. This...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.08.006
更新日期:2008-06-01 00:00:00
abstract::Fluorescence imaging of calcium ions (Ca(2+)) has become an essential technique for investigation of signaling pathways involving Ca(2+) as a second messenger. But, Ca(2+) signaling is involved in many biological phenomena, and therefore simultaneous visualization of Ca(2+) and other biomolecules (multicolor imaging) ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2016.06.002
更新日期:2016-10-01 00:00:00
abstract::The highly selective fluorescent Ca2+ indicator 'quin 2' has been loaded into ram and boar spermatozoa as the acetoxymethyl ester, 'quin 2/AM', which is hydrolysed and trapped in the cytoplasm. Loadings of several mM were not toxic to spermatozoa as judged by motility. Fluorescence measurements (mean +/- S.E.M.) indic...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(88)90037-1
更新日期:1988-02-01 00:00:00
abstract::Agonist induced increases in intracellular free calcium, [Ca2+]i, were measured in single Fura-2 loaded bovine aortic endothelial (BAE) cells by dual wavelength microspectrofluorimetry. Low doses of ATP (less than 10 microM) induced complex changes in [Ca2+]i. These changes usually consisted of a large initial transie...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90011-g
更新日期:1992-04-01 00:00:00
abstract::In smooth muscle, Ca(2+) regulates cell division, growth and cell death as well as providing the main trigger for contraction. Ion channels provide the major access route to elevate the cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) in smooth muscle by permitting Ca(2+) entry across the plasma membrane and release of ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.05.010
更新日期:2007-10-01 00:00:00
abstract::The release of Ca2+ ions from the sarcoplasmic reticulum through ryanodine receptor calcium release channels represents the critical step linking electrical excitation to muscular contraction in the heart and skeletal muscle (excitation-contraction coupling). Two small Ca2+ binding proteins, S100A1 and calmodulin, hav...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.06.001
更新日期:2011-10-01 00:00:00
abstract::We investigated whether the expression of human alpha-parvalbumin affects depolarization-induced elevations of the cytosolic free calcium concentration ([Ca2+]i) in human neuroblastoma SKNBE2 cells. A full length human parvalbumin cDNA was cloned by PCR from human cerebellum and transiently transfected into SKNBE2 cel...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90062-7
更新日期:1996-06-01 00:00:00
abstract::The regulation of the Ca2+ -induced Ca2+ release (CICR) from intracellular stores is a critical step in the cardiac cycle. The inherent positive feedback of CICR should make it a self-regenerating process. It is accepted that CICR must be governed by some negative control, but its nature is still debated. We explore h...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.01.023
更新日期:2004-08-01 00:00:00
abstract::Endothelin-1 (ET-1), a potent vasoactive peptide with a pathogenic role in vascular diseases, has been shown to induce the activation of ERK1/2, PKB and the expression of a transcriptional regulator, the early growth response 1 (Egr-1), key mediators of hypertrophic and proliferative responses in vascular smooth muscl...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.10.002
更新日期:2013-12-01 00:00:00
abstract::The control of calcium homeostasis is essential for cell survival and is of crucial importance for several physiological functions. The discovery of the epithelial calcium channel Transient Receptor Potential Vaniloid (TRPV6) in intestine has uncovered important Ca(2+) absorptive pathways involved in the regulation of...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.02.007
更新日期:2008-11-01 00:00:00
abstract::Cyclic AMP regulates the late step of Ca²+-dependent exocytosis in many secretory cells through two major mechanisms: a protein kinase A-dependent and a cAMP-GEF/Epac-dependent pathway. We designed a protocol to characterize the role of these two cAMP-dependent pathways on the Ca²+ sensitivity and kinetics of regulate...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.12.005
更新日期:2011-02-01 00:00:00
abstract::We have constructed a stochastic model of the inositol 1,4,5-trisphosphate receptor-Ca2+ channel that is based on quantitative measurements of the channel's properties. It displays the observed dependence of the open probability of the channel with cytosolic [Ca2+] and [IP3] and gives values for the dwell times that a...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2003.10.004
更新日期:2004-05-01 00:00:00
abstract::Removal of the epithelium from preparations of guinea-pig airways in vitro increases the responsiveness of the smooth muscle of normal and ovalbumin-sensitized animals to a number of contractile agents. To determine if epithelium removal results in an increase in Ca2+ entry into the smooth muscle, the effect of removi...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90026-1
更新日期:1987-12-01 00:00:00
abstract::Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca2+ mobilizing second messenger which triggers Ca2+ release in both sea urchin egg homogenates and in mammalian cells. The NAADP binding protein has not been identified and the regulation of NAADP mediated Ca2+ release remains controversial. To address...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102060
更新日期:2019-11-01 00:00:00
abstract::Cell survival is promoted by the oncoprotein Bcl-2. Previous studies have established that one of the pro-survival actions of Bcl-2 is to reduce cellular fluxes of Ca2+ within cells. In particular, Bcl-2 has been demonstrated to inhibit the release of Ca2+ from the endoplasmic reticulum. However, the mechanism by whic...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.01.003
更新日期:2008-09-01 00:00:00
abstract::Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.01.018
更新日期:2005-05-01 00:00:00
abstract::When Xenopus oocytes injected with rat brain poly(A)+RNA were voltage-clamped in a recording solution containing Ca2+, a depolarization pulse induced a transient current, ICl(Ca), which reflects calmodulin-mediated opening of endogenous Cl- channels in response to a Ca2+ influx through Ca2+ channels of brain origin. I...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90008-i
更新日期:1990-04-01 00:00:00
abstract::Store-operated Ca2+ entry (SOCE), an important Ca2+ signaling pathway in non-excitable cells, regulates a variety of cellular functions. To study its physiological role, pharmacological tools, like 2-aminoethyl diphenylborinate (2-APB), are used to impact SOCE. 2-APB is one of the best characterized SOCE inhibitors. H...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2017.01.015
更新日期:2017-03-01 00:00:00
abstract::The properties of the Ca2+, Mg2+-ATPase of erythrocyte membranes from patients with cystic fibrosis (CF) were extensively compared to that of healthy controls. Following removal of an endogenous membrane inhibitor of the ATPase, activation of the enzyme by Ca2+, calmodulin, limited tryptic digestion or oleic acid, as ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(85)90010-7
更新日期:1985-06-01 00:00:00
abstract::The L-type Ca2+ channel (Ca(v)1.2) is the main pathway for trans-sarcolemmal (SL) Ca2+ influx in cardiac myocytes. To maintain Ca2+ homeostasis, chronic SL Ca(2+)-influx must be matched by chronic SL efflux. In this study we tested the hypothesis that chronic downregulation of SL Ca2+ entry regulates SL extrusion. We ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.01.004
更新日期:2008-10-01 00:00:00
abstract::Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90113-x
更新日期:1996-05-01 00:00:00
abstract::When the concentrations of either calcium or of magnesium in the culture medium were increased from the normal 0.6 and 1.0 mM to 1.8 and 2.5 mM respectively mitotic activity of rat thymic lymphocytes increased. Very high (10(-4)M) ouabain concentrations abolished these mitogenic actions whilst lower (10(-7) and 10(-11...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90044-1
更新日期:1983-02-01 00:00:00