Abstract：
:The properties of the Ca2+, Mg2+-ATPase of erythrocyte membranes from patients with cystic fibrosis (CF) were extensively compared to that of healthy controls. Following removal of an endogenous membrane inhibitor of the ATPase, activation of the enzyme by Ca2+, calmodulin, limited tryptic digestion or oleic acid, as well as inhibition by trifluoperazine, were studied. The only properties found to be significantly different (CF cells vs controls) were calmodulin-stimulated peak activity (90 vs 101, P less than 0.02) and trypsin-activated peak activity (92 vs 102, P less than 0.02). No significant difference could be measured in the steady-state Ca2+-dependent phosphorylation of CF and control erythrocyte membranes indicating similar numbers of enzyme molecules per cell. The functional state of Ca2+ homeostasis in intact erythrocytes was investigated by measuring the resting cytosolic free Ca2+ levels using quin-2. Both CF and control erythrocytes maintained cytosolic free Ca2+ between 20 to 30 nM. Addition of 50 uM trifluoperazine resulted in an increase in erythrocyte cytosolic free Ca2+ to about 50 nM in both CF and control cells. Estimates of erythrocyte membrane permeability using the steady-state uptake of 45Ca into intact erythrocytes revealed no differences between CF and control cells. These results confirm that there is a small decrease in the calmodulin-stimulated activity of the erythrocyte Ca2+, Mg2+-ATPase in CF. However, this deficit is apparently not large enough to impair the ability of the CF erythrocyte to maintain normal resting levels of cytosolic free Ca2+.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Waller RL,Johnson LR,Brattin WJ,Dearborn DGdoi
10.1016/0143-4160(85)90010-7subject
Has Abstractpub_date
1985-06-01 00:00:00pages
245-64issue
3eissn
0143-4160issn
1532-1991pii
0143-4160(85)90010-7journal_volume
6pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Maitotoxin (MTX), a water-soluble polyether obtained from the marine dinoflagellate Gambierdiscus toxicus increased intracellular calcium in a concentration-dependent manner in fibroblasts obtained from human skin. The effect of this toxin was both saturable and of high affinity, showing an apparent half activation co...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90087-7
更新日期：1997-07-01 00:00:00
abstract：:Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(86)90042-4
更新日期：1986-12-01 00:00:00
abstract：:Vesl/Homer proteins physically link proteins that mediate cellular signaling [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851] and thereby influence cellular function [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962]. A previous study reported that Vesl-1L/Homer-1c ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00082-4
更新日期：2003-08-01 00:00:00
abstract：:Smooth muscle contraction is regulated by changes in cytosolic Ca(2+) concentration ([Ca(2+)](i)). In response to stimulation, Ca(2+) increase in a single cell can propagate to neighbouring cells through gap junctions, as intercellular Ca(2+) waves. To investigate the mechanisms underlying Ca(2+) wave propagation betw...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.08.001
更新日期：2011-11-01 00:00:00
abstract：:Although disturbance of cardiac Ca2+ regulation is involved in the pathophysiology of iron-overload cardiomyopathy, the obvious mechanisms involved in the dysregulation of iron-induced cardiac Ca2+ are unclear. Moreover, the roles of the iron chelator deferiprone and the T-type calcium channel blocker efonidipine on c...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.01.004
更新日期：2018-06-01 00:00:00
abstract：:Aging is a complex process that differentially impacts multiple cognitive, sensory, neuronal and molecular processes. Technological innovations now allow for parallel investigation of neuronal circuit function, structure and molecular composition in the brain of awake behaving adult mice. Thus, mice have become a crit...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2021.102358
更新日期：2021-01-23 00:00:00
abstract：:A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90027-6
更新日期：1995-10-01 00:00:00
abstract：:Localized subcellular changes in Ca(2+) serve as important cellular signaling elements, regulating processes as diverse as neuronal excitability and gene expression. Studies of cellular Ca(2+) signaling have been greatly facilitated by the availability of fluorescent Ca(2+) indicators. The respective merits of differe...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.10.003
更新日期：2015-12-01 00:00:00
abstract：:Turnover of the cartilage extracellular matrix depends exclusively on chondrocytes and varies in response to load and osmolarity fluctuations. Obesity can affect chondrocyte physiology; adipokines, insulin and proinflammatory cytokines levels are all altered in the obese and are related to matrix turnover impairments ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.10.006
更新日期：2014-12-01 00:00:00
abstract：:Specific Ca(2+) homeostatic system appeared very early in the history of the cell, as a survival system preventing Ca(2+)-mediated cell damage. This homeostatic system produced a steep ( approximately 20,000 times) concentration gradient between extracellular and intracellular compartments, which has both survival imp...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2007.05.001
更新日期：2007-10-01 00:00:00
abstract：:When Xenopus oocytes injected with rat brain poly(A)+RNA were voltage-clamped in a recording solution containing Ca2+, a depolarization pulse induced a transient current, ICl(Ca), which reflects calmodulin-mediated opening of endogenous Cl- channels in response to a Ca2+ influx through Ca2+ channels of brain origin. I...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90008-i
更新日期：1990-04-01 00:00:00
abstract：:Katz and co-workers showed that Ca(2+) triggers exocytosis. The existence of sub-micrometer domains of greater than 100 microM [Ca(2+)](i) was postulated on theoretical grounds. Using a modified, low-affinity aequorin, Llinas et al. were the first to demonstrate the existence of Ca(2+) 'microdomains' in squid presynap...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.007
更新日期：2006-11-01 00:00:00
abstract：:The regulation of intracellular Ca(2+) signalling by phosphorylation processes remains poorly defined, particularly with regards to tyrosine phosphorylation. Evidence from non-excitable cells implicates tyrosine phosphorylation in the activation of so-called store-operated Ca(2+) channels (SOCCs), but their involvemen...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2005.06.012
更新日期：2005-09-01 00:00:00
abstract：:Parvalbumin (PA) is a Ca(2+)-binding protein of vertebrates massively expressed in tissues with high oxygen uptake and respectively elevated level of reactive oxygen species (ROS). To characterize antioxidant properties of PA, antioxidant capacity (AOC) of intact rat α-PA has been explored. ORAC, TEAC and hydrogen per...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.03.001
更新日期：2014-05-01 00:00:00
abstract：:Intracellular calcium signals activated by growth factors in endothelial cells during angiogenesis regulate cytosolic and nuclear events involved in survival, proliferation and motility. Among the intracellular messengers released after proangiogenic stimulation (bFGF, VEGF), arachidonic acid (AA), nitric oxide (NO) a...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.07.003
更新日期：2007-03-01 00:00:00
abstract：:Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90113-x
更新日期：1996-05-01 00:00:00
abstract：:Cell survival is promoted by the oncoprotein Bcl-2. Previous studies have established that one of the pro-survival actions of Bcl-2 is to reduce cellular fluxes of Ca2+ within cells. In particular, Bcl-2 has been demonstrated to inhibit the release of Ca2+ from the endoplasmic reticulum. However, the mechanism by whic...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.01.003
更新日期：2008-09-01 00:00:00
abstract：:Cooling can induce Ca(2+) signaling via activation of temperature-sensitive ion channels such as TRPM8, TRPA1 and ryanodine receptor channels. Here we have studied the mechanism of cooling-evoked Ca(2+) signaling in mouse olfactory ensheathing cells (OECs), a specialized type of glial cells in the olfactory nerve laye...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.04.017
更新日期：2012-08-01 00:00:00
abstract：:Several potentially life-threatening cardiovascular and respiratory disorders result in prolonged deprivation of oxygen, which in turn results in significant cellular adaptation, or remodelling. An important component of this functional adaptation arises as a direct consequence of altered ion channel expression by chr...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.02.005
更新日期：2004-09-01 00:00:00
abstract：:Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90060-4
更新日期：1994-03-01 00:00:00
abstract：:In many animals, a rise in intracellular calcium levels is the trigger for egg activation, the process by which an arrested mature oocyte transitions to prepare for embryogenesis. In nearly all animals studied to date, this calcium rise, and thus egg activation, is triggered by the fertilizing sperm. However in the in...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2012.11.008
更新日期：2013-01-01 00:00:00
abstract：:TRPC channels are a subset of the transient receptor potential (TRP) proteins widely expressed in mammalian cells. They are thought to be primarily involved in determining calcium or sodium entry and have broad-ranging functions that include regulation of cell proliferation, motility and contraction. The channels do n...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2009.02.006
更新日期：2009-06-01 00:00:00
abstract：:Human neutrophils, preloaded with the fluorescent probe, Fura-2, were exposed to Ca2+-releasing agents. The monitored traces of fluorescence were transformed by computer to cytosolic Ca2+ concentration ([ Ca2+]i). Due to quenching of Fura-2, the addition of Mn2+ enabled us to compute the cytosolic concentration of tot...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(89)90025-0
更新日期：1989-10-01 00:00:00
abstract：:The sternal epithelium of Porcellio scaber was used as a novel model to study the subcellular elemental distribution in control and Ca(2+)-transporting stages in situ. The anterior sternal epithelium (ASE) is specialized for transport of cuticular Ca to sternal CaCO(3) deposits during premolt, and from these deposits ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(02)00060-x
更新日期：2002-06-01 00:00:00
abstract：:In salivary acinar cells, muscarinic-induced fluid secretion is associated with a 1,4,5-IP3 induced increase in the cytosolic free Ca2+ concentration ([Ca2+]i), which in turn activates Ca(2+)-dependent K+ and Cl- channels that modulate the membrane potential. In the present study the influence of the membrane potentia...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90076-i
更新日期：1993-07-01 00:00:00
abstract：:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel playing a major role in Ca2+ signaling. Three isoforms of IP3R have been identified and most cell types express different proportions of each isoform. The DT40 B lymphocyte cell line lacking all three IP3R isoform...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2004.03.005
更新日期：2005-02-01 00:00:00
abstract：:The A23187 induced calcium uptake in ATP depleted cells was determined at pH 6.9 in the presence of trifluoperazine (TFP, 0.30 mM), compound 48/80 (0.89 mg/ml), 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8, 2.13 mM) and verapamil (1.81 mM). Apart from verapamil the drugs all increased the maximum rate of ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(84)90022-8
更新日期：1984-10-01 00:00:00
abstract：:Dendritic Ca2+ plays an important role not only in synaptic integration and synaptic plasticity, but also in dendritic excitability in midbrain dopamine neurons. However, the functional organization of dendritic Ca2+ signals in the dopamine neurons remains largely unknown. We therefore investigated dendritic Ca2+ sign...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.06.007
更新日期：2011-10-01 00:00:00
abstract：:The mechanism by which the depletion of intracellular Ca2+ stores stimulates Ca2+ influx is poorly understood. However, the coupling of depletion to influx is broken during mitosis [Preston, S.F. et. al., (1991) Cell Regul., 2, 915-925]. Thus, in interphase HeLa cells, activation of the histamine H1 receptor, or incub...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90042-5
更新日期：1993-05-01 00:00:00
