Abstract:
:The A23187 induced calcium uptake in ATP depleted cells was determined at pH 6.9 in the presence of trifluoperazine (TFP, 0.30 mM), compound 48/80 (0.89 mg/ml), 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8, 2.13 mM) and verapamil (1.81 mM). Apart from verapamil the drugs all increased the maximum rate of ionophore-mediated calcium flux by 50-60 per cent. After the ionophore addition some time elapsed before the calcium flux attained the maximum value, and this time dependence could be interpreted as a slow uptake of A23187 into the membrane: five seconds after the addition of A23187 half of the added ionophore was able to transport calcium through the membrane. The effect of pH on the ionophore-mediated calcium uptake was determined in the absence and presence of TFP. At pH 7.4 the maximum rate of calcium flux in the absence of TFP was two to three times higher than that at pH 6.9 and TFP increased the uptake rate by 98 per cent.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Foder B,Skibsted U,Scharff Odoi
10.1016/0143-4160(84)90022-8subject
Has Abstractpub_date
1984-10-01 00:00:00pages
441-50issue
5eissn
0143-4160issn
1532-1991pii
0143-4160(84)90022-8journal_volume
5pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::Peripheral astrocytic processes (PAPs) are highly motile structures that are strategically positioned in close proximity to synapses. Long-lasting PAP retraction in hypothalamus is known to alter synaptic transmission. The PAP motility is likely to be actin-based because they are known to contain actin-related protein...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.03.001
更新日期:2013-05-01 00:00:00
abstract::Several proteins have been identified that associate with calcium release channels and potentially regulate their function. Using tacrolimus as a pharmacological tool, we investigated whether the immunophilin FKBP12 modulates ryanodine receptor channels in intestinal smooth muscle. Results with PCR demonstrated the pr...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90078-6
更新日期:1997-12-01 00:00:00
abstract::Photolysis of caged calcium (Nitr5, Calbiochem) can be used to study calcium dependent processes such as excitation-contraction coupling and muscular mechanics. Expensive high energy light sources are routinely used for UV light exposure, but this study describes an alternative low cost xenon flash unit constructed in...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90017-5
更新日期:1994-05-01 00:00:00
abstract::In the presence of inositol (1,4,5)-trisphosphate (InsP3) repetitive waves of elevated cytosolic free Ca2+ (Ca waves) that travel through cellular cytoplasm are observed. Investigation of this phenomenon stimulated the view of cellular cytoplasm as 'an excitable medium composed of Ca release processes (InsP3R), couple...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90019-1
更新日期:1994-09-01 00:00:00
abstract::Ca(2+) influx through voltage-gated Ca(2+) channels triggers the release of neurotransmitters at presynaptic terminals. Some sensory receptor cells in the peripheral auditory and visual systems have specialized synapses that express an electron-dense organelle called a synaptic ribbon. Like conventional synapses, ribb...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2012.06.004
更新日期:2012-09-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective death of motor neurons. Mutations in Cu/Zn superoxide dismutase-1 (SOD1) cause familial ALS but the molecular mechanisms whereby these mutations induce motor neuron death remain controversial. Here, we show that stable overex...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.02.004
更新日期:2011-04-01 00:00:00
abstract::Voltage activated L-type Ca(2+) channels are the principal Ca(2+) channels in intestinal smooth muscle cells. They comprise the ion conducting Ca(V)1 pore and the ancillary subunits alpha(2)delta and beta. Of the four Ca(V)beta subunits Ca(V)beta(3) is assumed to be the relevant Ca(V)beta protein in smooth muscle. In ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.04.013
更新日期:2007-10-01 00:00:00
abstract::We analyzed spatio-temporal characteristics of Ca2+ transients in the cytosol and the nucleus of cultured neonatal rat heart cells using confocal imaging with Indo-1 and Fluo-3. In resting heart muscle cells, nuclear [Ca2+] was maintained lower than the cytosolic level. The rise in nuclear [Ca2+], during either E-C co...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90031-4
更新日期:1995-03-01 00:00:00
abstract::For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed he...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(85)90008-9
更新日期:1985-06-01 00:00:00
abstract::Studies were conducted to examine the possible involvement of Na+-Ca2+ exchanger in determining the magnitude of the endothelin-1 (ET-1)-receptor-mediated calcium signal in porcine lens epithelial cells. Cytoplasmic calcium concentration was measured in primary cultured cells loaded with Fura-2. ET-1 (100 nM) caused c...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(03)00085-x
更新日期:2003-09-01 00:00:00
abstract::In previous studies we have characterised various properties of capacitative Ca2+ entry (CCE) in different epithelia. After Ca2+ store depletion with PLC/InsP3-coupled agonists or by inhibition of store Ca2+ uptake, with for example thapsigargin, Ca2+ influx is activated. This leads to a sustained cellular response (e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90112-3
更新日期:1997-08-01 00:00:00
abstract::The importance of Ca(2+) signaling in astrocytes is undisputed but a potential role of Ca(2+) influx via L-channels in the brain in vivo is disputed, although expression of these channels in cultured astrocytes is recognized. This study shows that an increase in free cytosolic Ca(2+) concentration ([Ca(2+)]i) in astro...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.08.002
更新日期:2013-11-01 00:00:00
abstract::A procedure for calibration of fluorescence signals from adult rat heart cells loaded with the -AM ester of fura-2 is described. Calibration is complicated by dye compartmentation and potentially incomplete dye hydrolysis. These problems were overcome by subtracting from fluorescence transients the non-cytosolic (mito...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90050-1
更新日期:1998-10-01 00:00:00
abstract::Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90070-6
更新日期:1996-04-01 00:00:00
abstract::Studies of Ca2+ transport pathways in exocrine gland cells have been useful, chiefly because of the polarized nature of the secretory epithelial cells. In pancreatic acinar cells, for example, Ca2+ reloading of empty intracellular stores can occur solely via Ca2+ entry through the basal part of the plasma membrane. On...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(03)00047-2
更新日期:2003-05-01 00:00:00
abstract::In smooth muscle, Ca(2+) regulates cell division, growth and cell death as well as providing the main trigger for contraction. Ion channels provide the major access route to elevate the cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) in smooth muscle by permitting Ca(2+) entry across the plasma membrane and release of ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.05.010
更新日期:2007-10-01 00:00:00
abstract::Cyclic AMP regulates the late step of Ca²+-dependent exocytosis in many secretory cells through two major mechanisms: a protein kinase A-dependent and a cAMP-GEF/Epac-dependent pathway. We designed a protocol to characterize the role of these two cAMP-dependent pathways on the Ca²+ sensitivity and kinetics of regulate...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.12.005
更新日期:2011-02-01 00:00:00
abstract::Using the fluorescent Ca2+ probe Quin-2 it has been reported that cholera toxin (CT) and its B subunit (B-CT) increase cytosolic free Ca2+ concentration ([Ca2+]i) in entherocytes, thymocytes and fibroblasts. In this work we show, however, that the fluorescence increases of Quin-2-loaded cells (rat thymocytes, mouse sp...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90054-x
更新日期:1990-06-01 00:00:00
abstract::Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.03.009
更新日期:2007-08-01 00:00:00
abstract::Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.08.003
更新日期:2011-11-01 00:00:00
abstract::Dopamine is one of the key neurotransmitters in the central nervous system and plays an important role in physiological processes, as well as in the development of many diseases. Here we report a receptor-independent signalling pathway induced by dopamine in CNS neurons. In cultured neurons from midbrain, cortex and h...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.08.008
更新日期:2010-08-01 00:00:00
abstract::Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.06.002
更新日期:2008-03-01 00:00:00
abstract::Mechanically induced channel activities and increase of intracellular calcium ([Ca2+]i) in normal and cancerous murine mammary cells (MMT 060562) were investigated using the patch clamp technique and Fura-2 fluorescence. Both cell types showed similar properties. Upon mechanical stimulation, activation of the Ca(2+)-d...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90018-n
更新日期:1992-08-01 00:00:00
abstract::The secretion of adrenocorticotrophin (ACTH) from corticotropes is a key component in the endocrine response to stress. The resting potential of corticotropes is set by the basal activities of TWIK-related K(+) (TREK)-1 channel. Corticotrophin-releasing hormone (CRH), the major ACTH secretagogue, closes the background...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.12.007
更新日期:2012-03-01 00:00:00
abstract::In murine ventricular myocytes, activation of mechanosensitive ion channels (MSCs) includes activation of non-selective cation currents and deactivation of inwardly rectifying potassium currents. Using pharmacological inhibitors and knockout models, we analyzed signaling steps that are critical to transduce the mechan...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.06.002
更新日期:2009-01-01 00:00:00
abstract::Palmitoylation (S-acylation) is the reversible conjugation of a fatty acid (usually C16 palmitate) to intracellular cysteine residues of proteins via a thioester linkage. Palmitoylation anchors intracellular regions of proteins to membranes because the palmitoylated cysteine is recruited to the lipid bilayer. NCX1 is ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102158
更新日期:2020-03-01 00:00:00
abstract::The mammalian transient receptor potential (TRP) proteins consist of a superfamily of Ca2+-permeant non-selective cation channels with structural similarities to Drosophila TRP. The TRP superfamily can be divided into three major families, among them the "canonical TRP" family (TRPC). The seven protein products of the...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(03)00056-3
更新日期:2003-05-01 00:00:00
abstract::Fura-2 and BAPTA were previously shown to be competitive antagonists of inositol trisphosphate (InsP3) receptors, but for practical reasons the analyses were performed at pH 8.3. We recently developed a scintillation proximity assay (SPA) for pure cerebellar InsP3 receptors which allows low affinity interactions to be...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.1998.0016
更新日期:1999-02-01 00:00:00
abstract::Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.01.018
更新日期:2005-05-01 00:00:00
abstract::Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(03)00143-x
更新日期:2003-10-01 00:00:00