Abstract:
BACKGROUND:Previous studies suggest that CaMKII activity is required for frequency-dependent acceleration of relaxation (FDAR) in ventricular myocytes. We propose that the underlying mechanism involves CaMKII-dependent regulation of myofilament Ca(2+) sensitivity. METHODS AND RESULTS:Cardiac function was measured in mice using murine echo machine. [Ca(2+)]i and sarcomere length were measured by IonOptix Ca(2+) image system. Increasing pacing rate from 0.5 to 4 Hz in left ventricular myocytes induced frequency-dependent myofilament Ca(2+) desensitization (FDMCD) and FDAR. Acute inhibition of PKA or PKC had no effect, whereas CaMKII inhibition abolished both FDMCD and FDAR. Co-immunoprecipitation of CaMKII and troponin I (TnI) has been detected and CaMKII inhibition significantly reduced serine residue phosphorylation of TnI. Finally, chronic inhibition of CaMKII in vivo reduced TnI phosphorylation and abolished both FDAR and FDMCD, leading to impaired diastolic function. CONCLUSIONS:Our results suggest that CaMKII-dependent TnI phosphorylation is involved in FDMCD and the consequent FDAR and that CaMKII inhibition removes this mechanism and thus induces diastolic dysfunction.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Guilbert A,Lim HJ,Cheng J,Wang Ydoi
10.1016/j.ceca.2015.08.001subject
Has Abstractpub_date
2015-11-01 00:00:00pages
489-99issue
5eissn
0143-4160issn
1532-1991pii
S0143-4160(15)00127-Xjournal_volume
58pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::Changes in the concentration and spatial distribution of Ca(2+) ions in the cytoplasm constitute a ubiquitous intracellular signaling module in cellular physiology. With the advent of Ca(2+) dyes that allow direct visualization of Ca(2+) transients, combined with powerful experimental tools such as electrophysiologica...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2010.10.003
更新日期:2010-11-01 00:00:00
abstract::A synthetic peptide (CaMBP) matching amino acids 3614-3643 of the skeletal ryanodine receptor (RyR1) binds to both Ca2+-free calmodulin (CaM) and Ca2+-bound CaM with nanomolar affinity [J. Biol. Chem. 276 (2001) 2069]. We report here that CaMBP increases [3H]ryanodine binding to RyR1 in a dose- and Ca2+-dependent mann...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2003.09.002
更新日期:2004-02-01 00:00:00
abstract::This work shows, for the first time, a properly metabolically regulated squid nerve Na(+)/Ca(2+) exchanger (NCXSQ1) heterologous expressed in Saccharomyces cerevisiae. The exchanger was fused to the enhanced green fluorescence protein (eGFP) on its C-terminus and had two tags, a Strep-tag II and 6 histidines, added to...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.03.009
更新日期:2009-05-01 00:00:00
abstract::Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.08.003
更新日期:2011-11-01 00:00:00
abstract::The sphingosine derivatives sphingosylphosphorylcholine (SPC) and sphingosine-1-phosphate (S1P) caused a similar elevation of the intracellular Ca2+ concentration ([Ca2+]i) in an immortalized airway epithelial cell line (CFNP9o-) incubated in Ca(2+)-free medium. The maximal effect was obtained with 2 microM SPC and 0....
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90095-1
更新日期:1998-06-01 00:00:00
abstract::Increases in global Ca(2+) in the endothelium are a crucial step in releasing relaxing factors to modulate arterial tone. In the present study we investigated spontaneous Ca(2+) events in endothelial cells, and the contribution of smooth muscle cells to these Ca(2+) events, in pressurized rat mesenteric resistance art...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.11.012
更新日期:2008-08-01 00:00:00
abstract::Removal of the epithelium from preparations of guinea-pig airways in vitro increases the responsiveness of the smooth muscle of normal and ovalbumin-sensitized animals to a number of contractile agents. To determine if epithelium removal results in an increase in Ca2+ entry into the smooth muscle, the effect of removi...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90026-1
更新日期:1987-12-01 00:00:00
abstract::Calcium is a major intracellular signaling messenger in innate immune cells. Similar to other immune cell subsets, the majority of calcium entry into innate immune cells is induced by cell surface receptors that stimulate store-operated calcium entry through calcium-release activated calcium (CRAC) channels. Since the...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2019.01.003
更新日期:2019-03-01 00:00:00
abstract::The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees C. After two washe...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90002-9
更新日期:1987-08-01 00:00:00
abstract::Ca(2+) signals control DNA synthesis and repair, gene transcription, and other cell functions that occur within the nucleus. The nuclear envelope can store Ca(2+) and release it into the nucleus via either the inositol 1,4,5-trisphosphate receptor (InsP3R) or the ryanodine receptor (RyR). Furthermore, many cell types ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.09.010
更新日期:2006-01-01 00:00:00
abstract::The non-selective second messenger-gated cation channel TRPC6 (transient receptor potential canonical 6) is activated by diacylglycerols (DAG) in a PKC-independent manner and plays important roles in a variety of physiological processes and diseases. In order to facilitate novel therapies, the development of potent in...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2018.12.009
更新日期:2019-03-01 00:00:00
abstract::An initial model has been proposed to describe a mechanism for cytosolic calcium oscillations [Jafri MS. Vajda S. Pasik P. Gillo B. (1992) A membrane model for cytosolic calcium oscillations: a study using Xenopus oocytes. Biophys. J., 63, 235-246]. In this paper we extend our original model to include the effects of ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(05)80003-x
更新日期:1994-07-01 00:00:00
abstract::The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.03.004
更新日期:2014-07-01 00:00:00
abstract::In the brain, classical (canonical) transient receptor potential (TRPC) channels are thought to be involved in different aspects of neuronal development. We investigated the developmental expression profile of TRPC channels in rat cerebellum during the first 6 weeks after birth. TRPC3 expression is significantly up-re...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.11.002
更新日期:2007-07-01 00:00:00
abstract::The mammalian ryanodine receptor Ca2+ release channel (RyR) has a single conserved high affinity calmodulin (CaM) binding domain. However, the skeletal muscle RyR1 is activated and cardiac muscle RyR2 is inhibited by CaM at submicromolar Ca2+. This suggests isoform-specific domains are involved in RyR regulation by Ca...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2017.05.013
更新日期:2017-09-01 00:00:00
abstract::We present a new method for ratiometric Ca2+ measurements using indicators with excitation spectra in the visible range of wavelengths. Laser-scanning confocal microscopy was used to record intracellular Ca(2+)-signals with high temporal and spatial resolution in single cardiac myocytes. The patch-clamp technique was ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90040-d
更新日期:1993-05-01 00:00:00
abstract::Several potentially life-threatening cardiovascular and respiratory disorders result in prolonged deprivation of oxygen, which in turn results in significant cellular adaptation, or remodelling. An important component of this functional adaptation arises as a direct consequence of altered ion channel expression by chr...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2004.02.005
更新日期:2004-09-01 00:00:00
abstract::Mitochondrial Ca(2+) activates many processes, from mitochondrial metabolism to opening of the permeability transition pore (PTP) and apoptosis. However, there is considerable controversy regarding the free mitochondrial [Ca(2+)] ([Ca(2+)](M)) levels that can be attained during cell activation or even in mitochondrial...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.10.007
更新日期:2009-03-01 00:00:00
abstract::An influx of Ca2+ into red blood cells of the bivalve mollusc Noetia ponderosa occurs immediately following a hypo-osmotic stress. The volume recovery response to the stress is dependent upon [Ca2+]o and is inhibited by phenothiazines. The action of these drugs is on the amino acid regulation portion of the recovery r...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(89)90070-5
更新日期:1989-04-01 00:00:00
abstract::The purpose of this study was to invent an extracellular inhibitor selective for the plasma membrane Ca(2+) pump(s) (PMCA) isoform 1. PMCA extrude Ca(2+) from cells during signalling and homeostasis. PMCA isoforms are encoded by 4 genes (PMCA1-4). Pig coronary artery endothelium and smooth muscle express the genes PMC...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.10.008
更新日期:2010-12-01 00:00:00
abstract::We tested a mixture of Calcium-Green-1 (CG-1) and Brilliantsulfaflavine (BS) for dual excitation ratiometric measurements of the intracellular free calcium concentration ([Ca2+]i) in bovine adrenal chromaffin cells. Dyes were coloaded (without being molecularly linked to each other) in the whole-cell configuration of ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90090-2
更新日期:1998-07-01 00:00:00
abstract::Using the fluorescent Ca2+ probe Quin-2 it has been reported that cholera toxin (CT) and its B subunit (B-CT) increase cytosolic free Ca2+ concentration ([Ca2+]i) in entherocytes, thymocytes and fibroblasts. In this work we show, however, that the fluorescence increases of Quin-2-loaded cells (rat thymocytes, mouse sp...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90054-x
更新日期:1990-06-01 00:00:00
abstract::Cardiogenesis depends on a tightly regulated balance between proliferation and differentiation of cardiac progenitor cells (CPCs) and their cardiomyocyte descendants. While exposure of early mouse embryos to Ca(2+) channel antagonists has been associated with abnormal cardiac morphogenesis, less is known about the con...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.02.018
更新日期:2014-05-01 00:00:00
abstract::Antisense oligodeoxynucleotides (AS-ODNs) were used in combination with transient functional expression of the cardiac Na(+)-Ca2+ exchanger (NCX1) to correlate suppression of the Na(+)-Ca2+ exchange function with down-regulation of NCX1 protein expression. In a de-novo expression system (Sf9 cells), a decrease in both...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.10.006
更新日期:2005-03-01 00:00:00
abstract::The oscillations in cytosolic Ca2+ evoked in pancreatic exocrine acinar cells by submaximal concentrations of the two phosphoinositidase-coupled agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8) have very different temporal patterns. In the present study we use digital video imaging of Fura-2 fluore...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90096-o
更新日期:1993-11-01 00:00:00
abstract::Pancreatitis is an increasingly common disease that carries a significant mortality and which lacks specific therapy. Pathological calcium signalling is an important contributor to the initiating cell injury, caused by or acting through mitochondrial inhibition. A principal effect of disordered cell signalling and imp...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.11.013
更新日期:2008-07-01 00:00:00
abstract::The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(82)90004-5
更新日期:1982-08-01 00:00:00
abstract::A whole range of cell functions are regulated by the free cytosolic Ca(2+)concentration. Activator Ca(2+)from the extracellular space enters the cell through various types of Ca(2+)channels and sometimes the Na(+)/Ca(2+)-exchanger, and is actively extruded from the cell by Ca(2+)pumps and Na(+)/Ca(2+)-exchangers. Acti...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1054/ceca.2000.0131
更新日期:2000-07-01 00:00:00
abstract::The effect of Ca2+ ion concentration on the 25 hydroxylation of tritiated cholecalciferol (3HD3) was investigated using homogenates of ovine liver from vitamin D replete sheep. A significant decrease in the production of 25 hydroxycholecalciferol (25OHD3) was observed when the concentration of Ca2+ in the homogenate w...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90001-7
更新日期:1987-08-01 00:00:00
abstract::There is controversy as to whether TRP channels participate in mediating store-operated current (I(SOC)) and store-operated Ca(2+) entry (SOCE). Our recent study has demonstrated that TRPC1 forms heteromeric channels with TRPV4 in vascular endothelial cells and that Ca(2+) store depletion enhances the vesicle traffick...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.08.006
更新日期:2011-12-01 00:00:00