Abstract:
:Within the CNS, distinct neurons may rely on different processes to modulate cytosolic Ca2+ depending on the network developmental phase. In particular, in the immature spinal cord, synchronous electrical discharges are coupled with biochemical signals triggered by intracellular Ca2+ waves. Nevertheless, the presence of neuronal-specific Ca2+ elevations independent from synaptic activity within mammalian spinal networks has not yet been described. The present report is the first description of repetitive calcium events generated by discrete ventral spinal neurons maintained in organotypic culture during in vitro maturation stages crucial for network evolution. Ventral interneurons in one-third of slices displayed spontaneous intracellular calcium transients suppressed by calcium-free extracellular solution or by application of cobalt, and resistant to blockers of network activity like TTX, CNQX, APV, strychnine or bicuculline. Our data suggest a primary role for mitochondria in intracellular calcium oscillations, because CCCP, that selectively collapses the mitochondrial electrochemical gradient, eliminated the ability of these neurons to show activity-independent calcium oscillations. Likewise, CGP-37157, a blocker of mitochondrial Na+/Ca2+ exchanger, inhibited oscillations in the majority of neurons. We propose that spontaneous Ca2+ transients, dynamically regulated by mitochondria, occurred in a discrete cluster of interneurons possibly to guide the development of synaptic connections.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Fabbro A,Pastore B,Nistri A,Ballerini Ldoi
10.1016/j.ceca.2006.07.006subject
Has Abstractpub_date
2007-04-01 00:00:00pages
317-29issue
4eissn
0143-4160issn
1532-1991pii
S0143-4160(06)00149-7journal_volume
41pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::We previously found in single channel studies that ryanodine receptor (RyR) channel activity can be made insensitive to block by Mg2+ when terminal cisternae of sarcoplasmic reticulum, incorporated into planar bilayers, are treated with protein kinase A (PKA) or Ca2+/calmodulin dependent protein kinase type II (CamPK ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90064-0
更新日期:1995-09-01 00:00:00
abstract::Nanosecond-duration electric stimuli are distinguished by the ability to permeabilize intracellular membranes and recruit Ca2+ from intracellular stores. We quantified this effect in non-excitable cells (CHO) using ratiometric Ca2+ imaging with Fura-2. In a Ca(2+)-free medium, 10-, 60-, and 300-ns stimuli evoked Ca2+ ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.05.008
更新日期:2013-09-01 00:00:00
abstract::TRPM family (Transient receptor potential channels, M for melastatin) is a group of intrinsic plasma membrane ion channels which are widely expressed throughout human body. It has been identified as a potent entry point of working desperate diseases out in a new way with newfangled ideas and safer technological means....
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2019.02.011
更新日期:2019-05-01 00:00:00
abstract::Store-operated calcium entry (SOCE) is activated within the endoplasmic reticulum (ER)-plasma membrane (PM) junctions in the plasma membrane and involves assembly of the channels, Orai1 and TRPC1, with the regulatory protein, STIM1, in response to depletion of Ca(2+) in the ER. The dynamic assembly of the channel comp...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2015.03.008
更新日期:2015-10-01 00:00:00
abstract::The strength of the heart beat depends on the amplitude and time course of the transient increase in [Ca(2+)] in the myocytes with each cycle. [Na(+)](i) modulates cardiac contraction through its effect on the Ca(2+) flux through the Na/Ca exchanger. Cardiac excitation-contraction coupling has been postulated to occur...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2004.01.014
更新日期:2004-06-01 00:00:00
abstract::Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.06.002
更新日期:2008-03-01 00:00:00
abstract::The endoplasmic reticulum (ER) acts as a dynamic calcium store and is involved in the generation of specific patterns of calcium signals in neurons. Calcium is mobilized from the ER store by multiple signaling cascades, and neuronal activity is known to regulate ER calcium levels. We asked how neurons regulate ER calc...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.04.002
更新日期:2015-08-01 00:00:00
abstract::Atypical absence epilepsy (AAE) is an intractable disorder characterized by slow spike-and-wave discharges in electroencephalograms (EEGs) and accompanied by severe cognitive dysfunction and neurodevelopmental or neurological deficits in humans. Administration of the cholesterol biosynthesis inhibitor AY-9944 (AY) dur...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.04.001
更新日期:2015-05-01 00:00:00
abstract::Acidic organelles such as lysosomes serve as non-canonical Ca(2+) stores. The Ca(2+) mobilising messenger NAADP is thought to trigger local Ca(2+) release from such stores. These events are then amplified by Ca(2+) channels on canonical ER Ca(2+) stores to generate physiologically relevant global Ca(2+) signals. Coupl...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2015.03.006
更新日期:2015-10-01 00:00:00
abstract::The control of calcium homeostasis is essential for cell survival and is of crucial importance for several physiological functions. The discovery of the epithelial calcium channel Transient Receptor Potential Vaniloid (TRPV6) in intestine has uncovered important Ca(2+) absorptive pathways involved in the regulation of...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.02.007
更新日期:2008-11-01 00:00:00
abstract::The mammalian ryanodine receptor Ca2+ release channel (RyR) has a single conserved high affinity calmodulin (CaM) binding domain. However, the skeletal muscle RyR1 is activated and cardiac muscle RyR2 is inhibited by CaM at submicromolar Ca2+. This suggests isoform-specific domains are involved in RyR regulation by Ca...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2017.05.013
更新日期:2017-09-01 00:00:00
abstract::Parvalbumin (PA) is a Ca(2+)-binding protein of vertebrates massively expressed in tissues with high oxygen uptake and respectively elevated level of reactive oxygen species (ROS). To characterize antioxidant properties of PA, antioxidant capacity (AOC) of intact rat α-PA has been explored. ORAC, TEAC and hydrogen per...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.03.001
更新日期:2014-05-01 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is a progressive muscle disease with severe cardiac complications. It is believed that cellular oxidative stress and augmented Ca(2+) signaling drives the development of cardiac pathology. Some mitochondrial and metabolic dysfunctions have also been reported. Here we investigate cellu...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.04.006
更新日期:2015-08-01 00:00:00
abstract::Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90070-6
更新日期:1996-04-01 00:00:00
abstract::A whole range of cell functions are regulated by the free cytosolic Ca(2+)concentration. Activator Ca(2+)from the extracellular space enters the cell through various types of Ca(2+)channels and sometimes the Na(+)/Ca(2+)-exchanger, and is actively extruded from the cell by Ca(2+)pumps and Na(+)/Ca(2+)-exchangers. Acti...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1054/ceca.2000.0131
更新日期:2000-07-01 00:00:00
abstract::Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90074-4
更新日期:1998-01-01 00:00:00
abstract::The secretion of adrenocorticotrophin (ACTH) from corticotropes is a key component in the endocrine response to stress. The resting potential of corticotropes is set by the basal activities of TWIK-related K(+) (TREK)-1 channel. Corticotrophin-releasing hormone (CRH), the major ACTH secretagogue, closes the background...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.12.007
更新日期:2012-03-01 00:00:00
abstract::Alternative splicing of the sarco/endoplasmic reticulum (SERCA2) Ca2+ pump transcript generates the two isoforms: SERCA2a in left ventricular myocytes (LVM) and SERCA2b in most tissues. Nuclear protein extracts from left ventricular myocytes can cause a decay of the 3'-region of the SERCA2a. To determine if all the do...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.06.003
更新日期:2005-01-01 00:00:00
abstract::Localized subcellular changes in Ca(2+) serve as important cellular signaling elements, regulating processes as diverse as neuronal excitability and gene expression. Studies of cellular Ca(2+) signaling have been greatly facilitated by the availability of fluorescent Ca(2+) indicators. The respective merits of differe...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.10.003
更新日期:2015-12-01 00:00:00
abstract::Previously we demonstrated that stimulation of cultured neonatal rat ventricular myocytes by either alpha 1-adrenergic agonist or endothelin-1 resulted in a rapid formation of total inositolphosphates, although the levels of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate did not rise significantly...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90014-4
更新日期:1995-12-01 00:00:00
abstract::Removal of the epithelium from preparations of guinea-pig airways in vitro increases the responsiveness of the smooth muscle of normal and ovalbumin-sensitized animals to a number of contractile agents. To determine if epithelium removal results in an increase in Ca2+ entry into the smooth muscle, the effect of removi...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90026-1
更新日期:1987-12-01 00:00:00
abstract::Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.1999.0044
更新日期:1999-06-01 00:00:00
abstract::One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid met...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2016.12.008
更新日期:2017-06-01 00:00:00
abstract::The store-operated, calcium release-activated calcium current I(CRAC) is activated by the depletion of inositol 1,4,5-trisphosphate (IP(3))-sensitive stores. The significantly different dose-response relationships of IP(3)-mediated Ca(2+) release and CRAC channel activation indicate that I(CRAC) is activated by a func...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.12.001
更新日期:2009-04-01 00:00:00
abstract::In skeletal muscle excitation-contraction (EC) coupling the sarcolemmal L-type Ca(2+) channel or 1,4-dihydropyridine receptor (DHPR) transduces the membrane depolarization signal to the sarcoplasmic Ca(2+) release channel RyR1 via protein-protein interaction. While it is evident that the pore-forming and voltage-sensi...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.04.003
更新日期:2010-06-01 00:00:00
abstract::The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.03.004
更新日期:2014-07-01 00:00:00
abstract::Sarcoplasmic reticulum contains the internal Ca(2+) store in smooth muscle cells and its lumen appears to be a continuum that lacks diffusion barriers. Accordingly, the free luminal Ca(2+) level is the same all throughout the SR; however, whether the Ca(2+) buffer capacity is the same in all the SR is unknown. We have...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.07.005
更新日期:2009-09-01 00:00:00
abstract::Calcium ion is involved in diverse physiological and developmental pathways. One of the important roles of calcium is a signaling messenger, which regulates signal transduction in plants. CBL (calcineurin B-like protein) is one of the calcium sensors that specifically interact with a family of serine-threonine protein...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.05.003
更新日期:2014-08-01 00:00:00
abstract::Numerous lines of evidence indicate that nuclear calcium concentration ([Ca2+]n) may be controlled independently from cytosolic events by a local machinery. In particular, the perinuclear space between the inner nuclear membrane (INM) and the outer nuclear membrane (ONM) of the nuclear envelope (NE) likely serves as a...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102143
更新日期:2020-03-01 00:00:00
abstract::Ever since British Physician William Withering first described the use of foxglove extract for treatment of patients with congestive heart failure in 1785, cardiotonic steroids have been used clinically to treat heart failure and more recently atrial fibrillation. Due to their ability to bind and inhibit the ubiquitou...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102156
更新日期:2020-03-01 00:00:00