Abstract：
:Oestrogens are involved in the growth, development and homeostasis of a number of tissues. The physiological effects of these steroids are mediated by a ligand-inducible nuclear transcription factor, the oestrogen receptor (ER). Hormone binding to the ligand-binding domain (LBD) of the ER initiates a series of molecular events culminating in the activation or repression of target genes. Transcriptional regulation arises from the direct interaction of the ER with components of the cellular transcription machinery. Here we report the crystal structures of the LBD of ER in complex with the endogenous oestrogen, 17beta-oestradiol, and the selective antagonist raloxifene, at resolutions of 3.1 and 2.6 A, respectively. The structures provide a molecular basis for the distinctive pharmacophore of the ER and its catholic binding properties. Agonist and antagonist bind at the same site within the core of the LBD but demonstrate different binding modes. In addition, each class of ligand induces a distinct conformation in the transactivation domain of the LBD, providing structural evidence of the mechanism of antagonism.
journal_name
Naturejournal_title
Natureauthors
Brzozowski AM,Pike AC,Dauter Z,Hubbard RE,Bonn T,Engström O,Ohman L,Greene GL,Gustafsson JA,Carlquist Mdoi
10.1038/39645subject
Has Abstractpub_date
1997-10-16 00:00:00pages
753-8issue
6652eissn
0028-0836issn
1476-4687journal_volume
389pub_type
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