Abstract：
:Nucleosomal DNA is arranged in a higher-order structure that presents a barrier to most cellular processes involving protein DNA interactions. The cellular machinery involved in sister chromatid cohesion, the cohesin complex, also requires access to the nucleosomal DNA to perform its function in chromosome segregation. The machineries that provide this accessibility are termed chromatin remodelling factors. Here, we report the isolation of a human ISWI (SNF2h)-containing chromatin remodelling complex that encompasses components of the cohesin and NuRD complexes. We show that the hRAD21 subunit of the cohesin complex directly interacts with the ATPase subunit SNF2h. Mapping of hRAD21, SNF2h and Mi2 binding sites by chromatin immunoprecipitation experiments reveals the specific association of these three proteins with human DNA elements containing Alu sequences. We find a correlation between modification of histone tails and association of the SNF2h/cohesin complex with chromatin. Moreover, we show that the association of the cohesin complex with chromatin can be regulated by the state of DNA methylation. Finally, we present evidence pointing to a role for the ATPase activity of SNF2h in the loading of hRAD21 on chromatin.
journal_name
Naturejournal_title
Natureauthors
Hakimi MA,Bochar DA,Schmiesing JA,Dong Y,Barak OG,Speicher DW,Yokomori K,Shiekhattar Rdoi
10.1038/nature01024keywords:
subject
Has Abstractpub_date
2002-08-29 00:00:00pages
994-8issue
6901eissn
0028-0836issn
1476-4687pii
nature01024journal_volume
418pub_type
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