Abstract：
:The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run. To achieve an approximately 100-fold increase in throughput over current Sanger sequencing technology, we have developed an emulsion method for DNA amplification and an instrument for sequencing by synthesis using a pyrosequencing protocol optimized for solid support and picolitre-scale volumes. Here we show the utility, throughput, accuracy and robustness of this system by shotgun sequencing and de novo assembly of the Mycoplasma genitalium genome with 96% coverage at 99.96% accuracy in one run of the machine.
journal_name
Naturejournal_title
Natureauthors
Margulies M,Egholm M,Altman WE,Attiya S,Bader JS,Bemben LA,Berka J,Braverman MS,Chen YJ,Chen Z,Dewell SB,Du L,Fierro JM,Gomes XV,Godwin BC,He W,Helgesen S,Ho CH,Irzyk GP,Jando SC,Alenquer ML,Jarvie TP,Jirage Kdoi
10.1038/nature03959keywords:
subject
Has Abstractpub_date
2005-09-15 00:00:00pages
376-80issue
7057eissn
0028-0836issn
1476-4687pii
nature03959journal_volume
437pub_type
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