Abstract:
:Enhancers increase the frequency of transcription initiation from linked promoter elements, most probably as a result of the binding of specific proteins to the enhancer. The polyomavirus early region is expressed in differentiated mouse cells but not in undifferentiated embryonal carcinoma (EC) cells. This host range is a function of the enhancer because polyomavirus mutants selected for growth in EC cells have mutations in the enhancer and the host range is reproduced in transfection assays using the mutant enhancers. To understand the basis for this alteration in enhancer function, we have assayed extracts of EC cells for proteins that can interact with this sequence. We have detected a protein, present in a variety of cells, that can bind to the F441 mutant sequence, but binds only very poorly to the wild-type sequence. We conclude that this sequence alteration has probably generated a binding site for a positive-acting factor that allows the enhancer to function.
journal_name
Naturejournal_title
Natureauthors
Kovesdi I,Satake M,Furukawa K,Reichel R,Ito Y,Nevins JRdoi
10.1038/328087a0subject
Has Abstractpub_date
1987-07-02 00:00:00pages
87-9issue
6125eissn
0028-0836issn
1476-4687journal_volume
328pub_type
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