Abstract:
:In order to study the mechanism of intracellular sorting and processing of the Alzheimer's disease amyloid precursor protein (APP), we deleted two potential N-linked glycosylation sites of APP by site-directed mutagenesis. Substitution of alanines for the critical asparagine residues Asn467 and Asn496 was performed. Wild-type and mutant APPs were expressed in COS-1 cells by cDNA transfection and the expressed of the protein and secretion of N-terminal large fragment was observed. The initial secretion of the mutant APP appeared to be slow compared with wild-type. In addition, we found that a distinct APP fragment, the cytosolic form, is transiently increased in the cytosol fraction of COS-1 cells. These results suggest that aberrant processing occurs following the expression of a mutant APP with Ala substituted for Asn, and that glycosylation may modulate the intracellular sorting of APP.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Yazaki M,Tagawa K,Maruyama K,Sorimachi H,Tsuchiya T,Ishiura S,Suzuki Kdoi
10.1016/s0304-3940(96)13285-7subject
Has Abstractpub_date
1996-12-27 00:00:00pages
57-60issue
1eissn
0304-3940issn
1872-7972pii
S0304-3940(96)13285-7journal_volume
221pub_type
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90075-5
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更新日期:2017-06-09 00:00:00
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:1991-03-11 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2017-08-24 00:00:00
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更新日期:1999-04-30 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2009-06-05 00:00:00
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更新日期:2006-09-11 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:1979-08-01 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:1990-07-31 00:00:00
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