Abstract：
:Apoptosis has been described as programmed active cell death. Overexpression of the c-Myc gene has been shown to induce apoptosis in Rat1A/Myc-ER cells when combined with a growth blocking agent. In the present study, we tested whether 4-Hydroxynonenal (HNE) or N-acetyl-L-cysteine (LNAC) affect apoptotic death in serum deprived Rat1A/Myc-ER cells caused by the c-Myc protein. The incidence of apoptosis in growth arrested cells was confirmed photographically and by ladder pattern formation seen on agarose gel electrophoresis. To study the effect of HNE and LNAC, the cells were cultured in 0.5% serum and treated with various concentrations of HNE and LNAC, c-Myc was then activated by addition of 2 microM beta-estradiol. Two separate parameters were used to determine the rate of cell death. In the first, the amount of DNA isolated from dying cells was compared by UV absorption. As the second parameter, the cell numbers were determined by counting. Our results showed that 1 microM of HNE has no effect on apoptotic cell death. However, 10 microM HNE decreased the amount of DNA isolated while increasing the cell count 14% relative to cells treated only with beta-estradiol. In addition, we found that various concentrations of the antioxidant LNAC does not protect cells from c-Myc induced death. However, it supports cell survival in 0.5% serum and causes an increase in cell number.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Yildiz D,Erçal N,Frank RL,Matthews RHdoi
10.1016/s0378-4274(96)03816-7subject
Has Abstractpub_date
1996-12-31 00:00:00pages
215-21issue
3eissn
0378-4274issn
1879-3169pii
S0378427496038167journal_volume
89pub_type
杂志文章abstract：:At the present work, different groups of rainbow trouts have been intraperitoneally treated with a polychlorinated biphenyls commercial mixture (Arochlor-1254) at three doses (5, 20 and 70 mg PCBs/ kg body weight) and they have been sampled at 0 (injection moment), 3, 6 and 9 weeks after xenobiotic exposure. Purificat...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(02)00284-9
更新日期：2002-12-15 00:00:00
abstract：:The gut microbiome has tremendous potential to impact health and disease. Various environmental toxicants, including insecticides, have been shown to alter gut microbiome community structures. However, the mechanism that compositionally and functionally regulates gut microbiota remains unclear. Quorum sensing is known...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.10.023
更新日期：2018-02-01 00:00:00
abstract：:Aminoglycosides have detrimental effects on the hair cells of the inner ear, yet these agents indisputably are one of the cornerstones in antibiotic therapy. Hence, there is a demand for strategies to prevent aminoglycoside-induced ototoxicity, which are not available today. In vitro data suggests that the pleiotropic...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2018.04.007
更新日期：2018-07-01 00:00:00
abstract：:Lead (Pb) alters the susceptibility to different pathogens suggesting that macrophage-mediated defense mechanisms, through activation of toll-like receptors (TLRs), may be affected by Pb. The aim of this study was to test whether activation of TLR4 is a targeted molecule to the effect of environmentally relevant Pb co...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2012.09.009
更新日期：2012-11-15 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2009.03.011
更新日期：2009-07-24 00:00:00
abstract：:Perfluorooctanoic acid (PFOA) is a stable man-made compound with many industrial and commercial uses. Recently, however, concern has been raised that it may induce various toxicological effects such as hepatotoxicity, immunotoxicity, and developmental toxicity. Because levels of circulating microRNAs (miRNAs) can be a...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2013.10.017
更新日期：2014-01-03 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(02)00050-4
更新日期：2002-05-28 00:00:00
abstract：:Oxidative damage plays a key role in septic shock induced by lipopolysaccharide (LPS) which is known to enhance the formation of reactive oxygen species (ROS). In this study, biochemical parameters indicative of oxidative stress were tested in the rat heart following LPS challenge, with and without pretreatment with t...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(01)00369-1
更新日期：2001-08-06 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(87)90042-7
更新日期：1987-03-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(85)90162-6
更新日期：1985-08-01 00:00:00
abstract：:Effects of sublethal concentration (0.8 of LC50 96 h) of nickel sulphate (NiSO4 X 7H2O) on muscle glycogen and blood lactic acid levels of a freshwater teleost, Colisa fasciatus, at time intervals from 3-96 h have been recorded. Muscle glycogen levels showed a general decrease at all time intervals compared with contr...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(84)90193-0
更新日期：1984-01-01 00:00:00
abstract：:In recent years, the detrimental effects of high iodine on intelligence are gaining tons of attention, but the relationship between high iodine and neurotoxicity is controversial. This study aimed to explore whether high iodine intake may impair intelligence and the roles of apoptosis and autophagy in high iodine-indu...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2018.08.020
更新日期：2018-11-01 00:00:00
abstract：:Ursolic acid (UA), a pentacyclic triterpene acid, is reported to have inducing activity of hepatic metallothionein (MT) which responsible for the detoxification of heavy metals; however, the mechanism underlying its effects is poorly understood. To further determine the underlying mechanism of UA, this study investiga...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2004.11.002
更新日期：2005-03-15 00:00:00
abstract：:The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is the most potent risk factor among tobacco-related carcinogens in lung cancer progression and outcomes. Although genetic mutations and chromosome instability have been detected in NNK-induced lung tumors, the oncogenic mechanisms o...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.09.019
更新日期：2017-11-05 00:00:00
abstract：:Chlordecone is an organochlorine used in the 1970's as a pesticide in banana plantations. It has a long half-life in the soil and can potentially contaminate humans and animals through food. Chlordecone targets, and mainly accumulates in, the liver, leading to hepatomegaly and neurological signs in mammals. Chlordecon...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2018.09.014
更新日期：2018-12-15 00:00:00
abstract：:We recently showed in a rat model of dichromate-induced acute renal failure (ARF) that the elimination but not the distribution of pralidoxime was altered resulting in sustained plasma pralidoxime concentrations. The aim of this study was to compare the efficiency of pralidoxime in normal and acute renal failure rats ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2009.04.020
更新日期：2009-08-25 00:00:00
abstract：:This study involved an attempt to establish a new photosafety screening system for dermally-applied chemicals consisting of a reactive oxygen species (ROS) assay and an in vitro skin permeation test. The ROS assay was undertaken to evaluate photoreactivity of six test compounds, acridine (ACD), furosemide (FSM), hexac...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2019.09.016
更新日期：2019-12-15 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(82)90160-6
更新日期：1982-05-01 00:00:00
abstract：:A detailed characterisation of the biotransformation pathways followed by the chemical of interest is, evidently, of primordial importance to design reliable biomonitoring programs. This short overview discusses some recent studies that are of potential interest for the development of new avenues of research. Four top...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(99)00077-6
更新日期：1999-09-05 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章,评审
doi：10.1016/0378-4274(94)90184-8
更新日期：1994-07-01 00:00:00
abstract：:L-Carnitine is a critical metabolite indispensable for the metabolism of lipids as it facilitates fatty acid transport into the mitochondrion where β-oxidation occurs. Human astrocytes (CCF-STTG1 cells) and hepatocytes (HepG2 cells) exposed to aluminum (Al) and hydrogen peroxide (H₂O₂), were characterized with lower l...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2011.03.019
更新日期：2011-06-24 00:00:00
abstract：:DNA-damaging agents are commonly used as anticancer therapeutics. Unfortunately, such drugs induced DNA damages as well as DNA repair are important in mediating drug resistance to cancer treatments. To evaluate changes in DNA repair proteins that occur in DNA damage agent treatment, we challenged human A549 lung adeno...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2011.06.028
更新日期：2011-09-10 00:00:00
abstract：:Nano-materials are currently being used in a variety of fields. One of the concerns associated with their use is their potential to harm human health. In an attempt to identify genes expressed differently in human lung cells (WI-26 VA4) exposed to nanosized (45 nm in diameter) PAMAM (polyamidoamine) dendrimers, we obs...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2009.07.018
更新日期：2009-10-28 00:00:00
abstract：:In vivo administration of 3-methylcholanthrene (MC) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) produced much higher hepatic microsomal aryl hydrocarbon hydroxylase (AHH) induction than 7,8-benzoflavone (7,8-BF) in both aromatic hydrocarbon (Ah)-responsive and nonresponsive strains of mice. Simultaneous treatment o...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(93)90095-f
更新日期：1993-02-01 00:00:00
abstract：:Both arsenite and benzo(a)pyrene (BaP) are known human carcinogens. Studies on the mode-of-action of arsenite indicate that it can also act as co-carcinogen or as a cancer promoter, and that it can facilitate progression of cancers. Some studies on development of lung cancers have suggested a synergism between arsenit...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2013.01.004
更新日期：2013-04-12 00:00:00
abstract：:The effect of chlordimeform (CDM) treatment on the hepatic microsomal drug metabolizing enzymes was examined in male and female rats following either acute or repeated treatment. After acute administration of chlordimeform (100 mg/kg, i.p., 1 hour before killing) differential effects were observed in various parameter...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(83)90072-3
更新日期：1983-08-01 00:00:00
abstract：:A toxicogenomics approach was applied to assess the usefulness of the mouse cytotoxic T cell line CTLL-2 for in vitro immunotoxicity testing. CTLL-2 cells were exposed for 6 h to two model immunotoxic compounds: (1) the mycotoxin deoxynivalenol (DON, 1 and 2 μM), a ribotoxic stress inducer, and (2) the organotin compo...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2012.12.005
更新日期：2013-02-13 00:00:00
abstract：:The effect of salicylate, the active metabolite of aspirin (acetyl salicylic acid) in the presence of Ca2+ and phosphate on mitochondrial permeability transition (MPT) was studied. MPT is often associated with opening of a Ca2+ -induced pore. The opening of this pore leads to swelling, loss of mitochondrial membrane p...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(98)00373-7
更新日期：1999-03-08 00:00:00
abstract：:To clarify a possible mechanism of zinc (Zn)-induced tolerance to cadmium (Cd) toxicity on bone formation, femurs from 9-day-old chick embryos were cultured for 6 days by the roller-tube method in the presence of Cd (2, 4 or 9 microM) and/or Zn (60 microM). Zn prevented a decrease in bone growth caused by Cd at 4 and ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(88)90149-x
更新日期：1988-11-01 00:00:00
abstract：:The study aimed to investigate the effects of egg white hydrolysate (EWH) on vascular disorders induced by mercury (Hg). For this, male Wistar rats were treated for 60days: Untreated (saline, i.m.); Mercury (HgCl2, i.m., 1st dose 4.6μg/kg, subsequent doses 0.07μg/kg/day); Hydrolysate (EWH, gavage, 1g/kg/day); Hydrolys...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.10.001
更新日期：2017-11-05 00:00:00
