Structure of the UmuD' protein and its regulation in response to DNA damage.


:For life to be sustained, mistakes in DNA repair must be tolerated when damage obscures the genetic information. In bacteria such as Escherichia coli, DNA damage elicits the well regulated 'SOS response'. For the extreme case of damage that cannot be repaired by conventional enzymes, there are proteins that allow the replication of DNA through such lesions, but with a reduction in the fidelity of replication. Essential proteins in this mutagenic process are RecA, DNA polymerase III, UmuD, UmuD' and UmuC (umu: UV mutagenesis). Regulation of this response involves a RecA-mediated self-cleavage of UmuD to produce UmuD'. To understand this system in more detail, we have determined the crystal structure of the E. coli UmuD' mutagenesis protein at 2.5 A resolution. Globular heads folded in an unusual Beta-structure associate to form molecular dimers, and extended amino-terminal tails associate to produce crystallized filaments. The structure provides insight into the mechanism of the self-cleavage reaction that UmuD-like proteins undergo as part of the global SOS response.






Peat TS,Frank EG,McDonald JP,Levine AS,Woodgate R,Hendrickson WA




Has Abstract


1996-04-25 00:00:00














  • RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis.

    abstract::Necroptosis has emerged as an important pathway of programmed cell death in embryonic development, tissue homeostasis, immunity and inflammation. RIPK1 is implicated in inflammatory and cell death signalling and its kinase activity is believed to drive RIPK3-mediated necroptosis. Here we show that kinase-independent s...


    pub_type: 杂志文章


    authors: Dannappel M,Vlantis K,Kumari S,Polykratis A,Kim C,Wachsmuth L,Eftychi C,Lin J,Corona T,Hermance N,Zelic M,Kirsch P,Basic M,Bleich A,Kelliher M,Pasparakis M

    更新日期:2014-09-04 00:00:00

  • Structure of tumour necrosis factor.

    abstract::Tumour necrosis factor is a trimeric molecule, each subunit of which consists of an antiparallel beta-sandwich. Individual subunits from the trimer by a novel edge-to-face packing of beta-sheets. A comparison of the subunit fold with that of other proteins reveals a remarkable similarity to the 'jelly-roll' structural...


    pub_type: 杂志文章


    authors: Jones EY,Stuart DI,Walker NP

    更新日期:1989-03-16 00:00:00

  • The confinement of Neptune's ring arcs by the moon Galatea.

    abstract::Neptune has five narrow ring arcs, spanning about 40 degrees in longitude, which are apparently confined against the rapid azimuthal and radial spreading that normally results from inter-particle collisions. A gravitational resonance based on the vertical motion of the nearby neptunian moon Galatea was proposed to exp...


    pub_type: 杂志文章


    authors: Namouni F,Porco C

    更新日期:2002-05-02 00:00:00

  • The microbiota regulate neuronal function and fear extinction learning.

    abstract::Multicellular organisms have co-evolved with complex consortia of viruses, bacteria, fungi and parasites, collectively referred to as the microbiota1. In mammals, changes in the composition of the microbiota can influence many physiologic processes (including development, metabolism and immune cell function) and are a...


    pub_type: 杂志文章


    authors: Chu C,Murdock MH,Jing D,Won TH,Chung H,Kressel AM,Tsaava T,Addorisio ME,Putzel GG,Zhou L,Bessman NJ,Yang R,Moriyama S,Parkhurst CN,Li A,Meyer HC,Teng F,Chavan SS,Tracey KJ,Regev A,Schroeder FC,Lee FS,Liston C

    更新日期:2019-10-01 00:00:00

  • Male development of chromosomally female mice transgenic for Sry.

    abstract::The initiation of male development in mammals requires one or more genes on the Y chromosome. A recently isolated gene, termed SRY in humans and Sry in mouse, has many of the genetic and biological properties expected of a Y-located testis-determining gene. It is now shown that Sry on a 14-kilobase genomic DNA fragmen...


    pub_type: 杂志文章


    authors: Koopman P,Gubbay J,Vivian N,Goodfellow P,Lovell-Badge R

    更新日期:1991-05-09 00:00:00

  • Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities.

    abstract::The spindle checkpoint prevents chromosome mis-segregation by delaying sister chromatid separation until all chromosomes have achieved bipolar attachment to the mitotic spindle. Its operation is essential for accurate chromosome segregation, whereas its dysregulation can contribute to birth defects and tumorigenesis. ...


    pub_type: 杂志文章


    authors: Stegmeier F,Rape M,Draviam VM,Nalepa G,Sowa ME,Ang XL,McDonald ER 3rd,Li MZ,Hannon GJ,Sorger PK,Kirschner MW,Harper JW,Elledge SJ

    更新日期:2007-04-19 00:00:00

  • Immunization to a syngeneic sarcoma by a monoclonal auto-anti-idiotypic antibody.

    abstract::Idiotypic networks regulate the immune response to a variety of antigens. Antibodies generated against other antibodies, called anti-idiotypic antibodies, can themselves mimic antigen and elicit a specific immune response. They have been shown to induce delayed-type hypersensitivity (DTH) to model antigens in the mous...


    pub_type: 杂志文章


    authors: Forstrom JW,Nelson KA,Nepom GT,Hellström I,Hellström KE

    更新日期:1983-06-16 00:00:00

  • Non-thermal melting in semiconductors measured at femtosecond resolution.

    abstract::Ultrafast time-resolved optical spectroscopy has revealed new classes of physical, chemical and biological reactions, in which directed, deterministic motions of atoms have a key role. This contrasts with the random, diffusive motion of atoms across activation barriers that typically determines kinetic rates on slower...


    pub_type: 杂志文章


    authors: Rousse A,Rischel C,Fourmaux S,Uschmann I,Sebban S,Grillon G,Balcou P,Förster E,Geindre JP,Audebert P,Gauthier JC,Hulin D

    更新日期:2001-03-01 00:00:00

  • Coexistence of Fermi arcs and Fermi pockets in a high-T(c) copper oxide superconductor.

    abstract::In the pseudogap state of the high-transition-temperature (high-T(c)) copper oxide superconductors, angle-resolved photoemission (ARPES) measurements have seen Fermi arcs-that is, open-ended gapless sections in the large Fermi surface-rather than a closed loop expected of an ordinary metal. This is all the more puzzli...


    pub_type: 杂志文章


    authors: Meng J,Liu G,Zhang W,Zhao L,Liu H,Jia X,Mu D,Liu S,Dong X,Zhang J,Lu W,Wang G,Zhou Y,Zhu Y,Wang X,Xu Z,Chen C,Zhou XJ

    更新日期:2009-11-19 00:00:00

  • Speciation in a ring.

    abstract::The evolutionary divergence of a single species into two has never been directly observed in nature, primarily because speciation can take a long time to occur. A ring species, in which a chain of intergrading populations encircles a barrier and the terminal forms coexist without interbreeding, provides a situation in...


    pub_type: 杂志文章


    authors: Irwin DE,Bensch S,Price TD

    更新日期:2001-01-18 00:00:00

  • Robotics: self-reproducing machines.

    abstract::Self-reproduction is central to biological life for long-term sustainability and evolutionary adaptation. Although these traits would also be desirable in many engineered systems, the principles of self-reproduction have not been exploited in machine design. Here we create simple machines that act as autonomous modula...


    pub_type: 杂志文章


    authors: Zykov V,Mytilinaios E,Adams B,Lipson H

    更新日期:2005-05-12 00:00:00

  • Atomic structure of the eukaryotic intramembrane RAS methyltransferase ICMT.

    abstract::The maturation of RAS GTPases and approximately 200 other cellular CAAX proteins involves three enzymatic steps: addition of a farnesyl or geranylgeranyl prenyl lipid to the cysteine (C) in the C-terminal CAAX motif, proteolytic cleavage of the AAX residues and methylation of the exposed prenylcysteine residue at its ...


    pub_type: 杂志文章


    authors: Diver MM,Pedi L,Koide A,Koide S,Long SB

    更新日期:2018-01-25 00:00:00

  • Continuous c-fos expression precedes programmed cell death in vivo.

    abstract::The development of a multicellular organism involves a delicate balance among the processes of proliferation, differentiation and death. Naturally occurring cell death aids tissue remodelling, eliminates supernumerary cell populations and provides structural elements such as hair and skin. In the nervous system, selec...


    pub_type: 杂志文章


    authors: Smeyne RJ,Vendrell M,Hayward M,Baker SJ,Miao GG,Schilling K,Robertson LM,Curran T,Morgan JI

    更新日期:1993-05-13 00:00:00

  • Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins.

    abstract::Post-translational modification (PTM) of proteins plays an important part in mediating protein interactions and/or the recruitment of specific protein targets. PTM can be mediated by the addition of functional groups (for example, acetylation or phosphorylation), peptides (for example, ubiquitylation or sumoylation), ...


    pub_type: 杂志文章


    authors: Ahel I,Ahel D,Matsusaka T,Clark AJ,Pines J,Boulton SJ,West SC

    更新日期:2008-01-03 00:00:00

  • The Drosophila claret segregation protein is a minus-end directed motor molecule.

    abstract::A product encoded at the claret locus in Drosophila is needed for normal chromosome segregation in meiosis in females and in early mitotic divisions of the embryo. The predicted amino-acid sequence of the segregation protein was shown recently to be strikingly similar to Drosophila kinesin heavy chain. We have express...


    pub_type: 杂志文章


    authors: Walker RA,Salmon ED,Endow SA

    更新日期:1990-10-25 00:00:00

  • Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid.

    abstract::Endophilin I is a presynaptic protein of unknown function that binds to dynamin, a GTPase that is implicated in endocytosis and recycling of synaptic vesicles. Here we show that endophilin I is essential for the formation of synaptic-like microvesicles (SLMVs) from the plasma membrane. Endophilin I exhibits lysophosph...


    pub_type: 杂志文章


    authors: Schmidt A,Wolde M,Thiele C,Fest W,Kratzin H,Podtelejnikov AV,Witke W,Huttner WB,Söling HD

    更新日期:1999-09-09 00:00:00

  • Peierls distortion as a route to high thermoelectric performance in In(4)Se(3-delta) crystals.

    abstract::Thermoelectric energy harvesting-the transformation of waste heat into useful electricity-is of great interest for energy sustainability. The main obstacle is the low thermoelectric efficiency of materials for converting heat to electricity, quantified by the thermoelectric figure of merit, ZT. The best available n-ty...


    pub_type: 杂志文章


    authors: Rhyee JS,Lee KH,Lee SM,Cho E,Kim SI,Lee E,Kwon YS,Shim JH,Kotliar G

    更新日期:2009-06-18 00:00:00

  • Publisher Correction: The effect of large-scale anti-contagion policies on the COVID-19 pandemic.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...


    pub_type: 杂志文章,已发布勘误


    authors: Hsiang S,Allen D,Annan-Phan S,Bell K,Bolliger I,Chong T,Druckenmiller H,Huang LY,Hultgren A,Krasovich E,Lau P,Lee J,Rolf E,Tseng J,Wu T

    更新日期:2020-09-01 00:00:00

  • Next-generation characterization of the Cancer Cell Line Encyclopedia.

    abstract::Large panels of comprehensively characterized human cancer models, including the Cancer Cell Line Encyclopedia (CCLE), have provided a rigorous framework with which to study genetic variants, candidate targets, and small-molecule and biological therapeutics and to identify new marker-driven cancer dependencies. To imp...


    pub_type: 杂志文章


    authors: Ghandi M,Huang FW,Jané-Valbuena J,Kryukov GV,Lo CC,McDonald ER 3rd,Barretina J,Gelfand ET,Bielski CM,Li H,Hu K,Andreev-Drakhlin AY,Kim J,Hess JM,Haas BJ,Aguet F,Weir BA,Rothberg MV,Paolella BR,Lawrence MS,Akbani R

    更新日期:2019-05-01 00:00:00

  • Journal blacklists: show your working.

    abstract:: ...


    pub_type: 社论



    更新日期:2018-10-01 00:00:00

  • Blazar spectral variability as explained by a twisted inhomogeneous jet.

    abstract::Blazars are active galactic nuclei, which are powerful sources of radiation whose central engine is located in the core of the host galaxy. Blazar emission is dominated by non-thermal radiation from a jet that moves relativistically towards us, and therefore undergoes Doppler beaming. This beaming causes flux enhancem...


    pub_type: 杂志文章


    authors: Raiteri CM,Villata M,Acosta-Pulido JA,Agudo I,Arkharov AA,Bachev R,Baida GV,Benítez E,Borman GA,Boschin W,Bozhilov V,Butuzova MS,Calcidese P,Carnerero MI,Carosati D,Casadio C,Castro-Segura N,Chen WP,Damljanovic G,D'

    更新日期:2017-12-21 00:00:00

  • A binary main-belt comet.

    abstract::Asteroids are primitive Solar System bodies that evolve both collisionally and through disruptions arising from rapid rotation. These processes can lead to the formation of binary asteroids and to the release of dust, both directly and, in some cases, through uncovering frozen volatiles. In a subset of the asteroids c...


    pub_type: 杂志文章


    authors: Agarwal J,Jewitt D,Mutchler M,Weaver H,Larson S

    更新日期:2017-09-20 00:00:00

  • Host-directed therapy of tuberculosis based on interleukin-1 and type I interferon crosstalk.

    abstract::Tuberculosis remains second only to HIV/AIDS as the leading cause of mortality worldwide due to a single infectious agent. Despite chemotherapy, the global tuberculosis epidemic has intensified because of HIV co-infection, the lack of an effective vaccine and the emergence of multi-drug-resistant bacteria. Alternative...


    pub_type: 杂志文章


    authors: Mayer-Barber KD,Andrade BB,Oland SD,Amaral EP,Barber DL,Gonzales J,Derrick SC,Shi R,Kumar NP,Wei W,Yuan X,Zhang G,Cai Y,Babu S,Catalfamo M,Salazar AM,Via LE,Barry CE 3rd,Sher A

    更新日期:2014-07-03 00:00:00

  • Venice anti-flood gates could wreck lagoon ecosystem.

    abstract:: ...


    pub_type: 新闻


    authors: Del Bello L

    更新日期:2018-12-01 00:00:00

  • Dicer recognizes the 5' end of RNA for efficient and accurate processing.

    abstract::A hallmark of RNA silencing is a class of approximately 22-nucleotide RNAs that are processed from double-stranded RNA precursors by Dicer. Accurate processing by Dicer is crucial for the functionality of microRNAs (miRNAs). The current model posits that Dicer selects cleavage sites by measuring a set distance from th...


    pub_type: 杂志文章


    authors: Park JE,Heo I,Tian Y,Simanshu DK,Chang H,Jee D,Patel DJ,Kim VN

    更新日期:2011-07-13 00:00:00

  • A single amino acid of the cholecystokinin-B/gastrin receptor determines specificity for non-peptide antagonists.

    abstract::The brain cholecystokinin-B/gastrin receptor (CCK-B/gastrin) has been implicated in mediating anxiety, panic attacks, satiety, and the perception of pain. The canine and human CCK-B/gastrin receptors share 90% amino-acid identity and have similar agonist affinities. These receptors can be selectively blocked by the no...


    pub_type: 杂志文章


    authors: Beinborn M,Lee YM,McBride EW,Quinn SM,Kopin AS

    更新日期:1993-03-25 00:00:00

  • Attenuation of Mengo virus through genetic engineering of the 5' noncoding poly(C) tract.

    abstract::The murine cardioviruses, such as the Mengo and encephalomyocarditis viruses, and the bovine aphthoviruses, such as foot-and-mouth disease virus, are distinguished among positive-strand RNA viruses by the presence of long homopolymeric poly(C) tracts within their 5' noncoding sequences. Although the specific lengths (...


    pub_type: 杂志文章


    authors: Duke GM,Osorio JE,Palmenberg AC

    更新日期:1990-02-01 00:00:00

  • Synthetic biology: engineering Escherichia coli to see light.

    abstract::We have designed a bacterial system that is switched between different states by red light. The system consists of a synthetic sensor kinase that allows a lawn of bacteria to function as a biological film, such that the projection of a pattern of light on to the bacteria produces a high-definition (about 100 megapixel...


    pub_type: 杂志文章


    authors: Levskaya A,Chevalier AA,Tabor JJ,Simpson ZB,Lavery LA,Levy M,Davidson EA,Scouras A,Ellington AD,Marcotte EM,Voigt CA

    更新日期:2005-11-24 00:00:00

  • Regulation of receptor-mediated endocytosis by Rho and Rac.

    abstract::Pinocytosis and membrane ruffling are among the earliest and most dramatic cellular responses to stimulation by growth factors or other mitogens. The small Ras-related G proteins Rho and Rac have a regulatory role in membrane ruffling and activated Rho has been shown to stimulate pinocytosis when microinjected into Xe...


    pub_type: 杂志文章


    authors: Lamaze C,Chuang TH,Terlecky LJ,Bokoch GM,Schmid SL

    更新日期:1996-07-11 00:00:00

  • Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.

    abstract::One goal of regenerative medicine, to use stem cells to replace cells lost by injury or disease, depends on producing an excess of the relevant cell for study or transplantation. To this end, the stepwise differentiation of stem cells into specialized derivatives has been successful for some cell types, but a major pr...


    pub_type: 杂志文章


    authors: Sneddon JB,Borowiak M,Melton DA

    更新日期:2012-11-29 00:00:00