Structure of the UmuD' protein and its regulation in response to DNA damage.


:For life to be sustained, mistakes in DNA repair must be tolerated when damage obscures the genetic information. In bacteria such as Escherichia coli, DNA damage elicits the well regulated 'SOS response'. For the extreme case of damage that cannot be repaired by conventional enzymes, there are proteins that allow the replication of DNA through such lesions, but with a reduction in the fidelity of replication. Essential proteins in this mutagenic process are RecA, DNA polymerase III, UmuD, UmuD' and UmuC (umu: UV mutagenesis). Regulation of this response involves a RecA-mediated self-cleavage of UmuD to produce UmuD'. To understand this system in more detail, we have determined the crystal structure of the E. coli UmuD' mutagenesis protein at 2.5 A resolution. Globular heads folded in an unusual Beta-structure associate to form molecular dimers, and extended amino-terminal tails associate to produce crystallized filaments. The structure provides insight into the mechanism of the self-cleavage reaction that UmuD-like proteins undergo as part of the global SOS response.






Peat TS,Frank EG,McDonald JP,Levine AS,Woodgate R,Hendrickson WA




Has Abstract


1996-04-25 00:00:00














  • Severe immunodeficiency disease induced by a defective murine leukaemia virus.

    abstract::Different classes of retroviruses have been shown to induce immunodeficiency diseases in various animal species. These animal models may provide an insight into our understanding of AIDS but, with the exception of one strain of feline leukaemia virus, the determinants of pathogenicity have not yet been mapped to these...


    pub_type: 杂志文章


    authors: Aziz DC,Hanna Z,Jolicoeur P

    更新日期:1989-04-06 00:00:00

  • Gene expression variability across cells and species shapes innate immunity.

    abstract::As the first line of defence against pathogens, cells mount an innate immune response, which varies widely from cell to cell. The response must be potent but carefully controlled to avoid self-damage. How these constraints have shaped the evolution of innate immunity remains poorly understood. Here we characterize the...


    pub_type: 杂志文章


    authors: Hagai T,Chen X,Miragaia RJ,Rostom R,Gomes T,Kunowska N,Henriksson J,Park JE,Proserpio V,Donati G,Bossini-Castillo L,Vieira Braga FA,Naamati G,Fletcher J,Stephenson E,Vegh P,Trynka G,Kondova I,Dennis M,Haniffa M,No

    更新日期:2018-11-01 00:00:00

  • Speciation in a ring.

    abstract::The evolutionary divergence of a single species into two has never been directly observed in nature, primarily because speciation can take a long time to occur. A ring species, in which a chain of intergrading populations encircles a barrier and the terminal forms coexist without interbreeding, provides a situation in...


    pub_type: 杂志文章


    authors: Irwin DE,Bensch S,Price TD

    更新日期:2001-01-18 00:00:00

  • Coexistence of Fermi arcs and Fermi pockets in a high-T(c) copper oxide superconductor.

    abstract::In the pseudogap state of the high-transition-temperature (high-T(c)) copper oxide superconductors, angle-resolved photoemission (ARPES) measurements have seen Fermi arcs-that is, open-ended gapless sections in the large Fermi surface-rather than a closed loop expected of an ordinary metal. This is all the more puzzli...


    pub_type: 杂志文章


    authors: Meng J,Liu G,Zhang W,Zhao L,Liu H,Jia X,Mu D,Liu S,Dong X,Zhang J,Lu W,Wang G,Zhou Y,Zhu Y,Wang X,Xu Z,Chen C,Zhou XJ

    更新日期:2009-11-19 00:00:00

  • Alternative transcription initiation leads to expression of a novel ALK isoform in cancer.

    abstract::Activation of oncogenes by mechanisms other than genetic aberrations such as mutations, translocations, or amplifications is largely undefined. Here we report a novel isoform of the anaplastic lymphoma kinase (ALK) that is expressed in ∼11% of melanomas and sporadically in other human cancer types, but not in normal t...


    pub_type: 杂志文章


    authors: Wiesner T,Lee W,Obenauf AC,Ran L,Murali R,Zhang QF,Wong EW,Hu W,Scott SN,Shah RH,Landa I,Button J,Lailler N,Sboner A,Gao D,Murphy DA,Cao Z,Shukla S,Hollmann TJ,Wang L,Borsu L,Merghoub T,Schwartz GK,Postow MA

    更新日期:2015-10-15 00:00:00

  • Hox gene expression in teleost fins and the origin of vertebrate digits.

    abstract::Hox genes are essential for growth and patterning of the tetrapod limb skeleton. Mice mutant for the Hoxd-13 gene have an important delay in morphogenesis owing to reduced proliferation. Based on the appearance of atavisms in such mice, we suggested that modifications of Hox gene regulation may have been a source of m...


    pub_type: 杂志文章


    authors: Sordino P,van der Hoeven F,Duboule D

    更新日期:1995-06-22 00:00:00

  • Molecular envoys pave the way for pancreatic cancer to invade the liver.

    abstract:: ...


    pub_type: 评论,新闻


    authors: Maitra A

    更新日期:2019-03-01 00:00:00

  • Dissociable neural mechanisms supporting visual short-term memory for objects.

    abstract::Using visual information to guide behaviour requires storage in a temporary buffer, known as visual short-term memory (VSTM), that sustains attended information across saccades and other visual interruptions. There is growing debate on whether VSTM capacity is limited to a fixed number of objects or whether it is vari...


    pub_type: 杂志文章


    authors: Xu Y,Chun MM

    更新日期:2006-03-02 00:00:00

  • Multiple spike-initiation zones in single neurons revealed by voltage-sensitive dyes.

    abstract::The primary function of the nerve cell is to process electrical signals. Over the past fifteen years there has been renewed interest in the detailed spatial analysis of signalling in individual neurons owing to experimental evidence that the regional electrical properties of neurons are complex. Thus the behaviour of ...


    pub_type: 杂志文章


    authors: Zecević D

    更新日期:1996-05-23 00:00:00

  • Mapping human visual cortex with positron emission tomography.

    abstract::Positron-emission tomography (PET) can localize functions of the human brain by imaging regional cerebral blood flow (CBF) during voluntary behaviour. Functional brain mapping with PET, however, has been hindered by PET's poor spatial resolution (typically greater than 1 cm). We have developed an image-analysis strate...


    pub_type: 杂志文章


    authors: Fox PT,Mintun MA,Raichle ME,Miezin FM,Allman JM,Van Essen DC

    更新日期:1986-10-30 00:00:00

  • The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.

    abstract::Aquifex aeolicus was one of the earliest diverging, and is one of the most thermophilic, bacteria known. It can grow on hydrogen, oxygen, carbon dioxide, and mineral salts. The complex metabolic machinery needed for A. aeolicus to function as a chemolithoautotroph (an organism which uses an inorganic carbon source for...


    pub_type: 杂志文章


    authors: Deckert G,Warren PV,Gaasterland T,Young WG,Lenox AL,Graham DE,Overbeek R,Snead MA,Keller M,Aujay M,Huber R,Feldman RA,Short JM,Olsen GJ,Swanson RV

    更新日期:1998-03-26 00:00:00

  • Chemical and behavioural characterization of the rabbit mammary pheromone.

    abstract::Mammals owe part of their evolutionary success to the harmonious exchanges of information, energy and immunity between females and their offspring. This functional reciprocity is vital for the survival and normal development of infants, and for the inclusive fitness of parents. It is best seen in the intense exchanges...


    pub_type: 杂志文章


    authors: Schaal B,Coureaud G,Langlois D,Giniès C,Sémon E,Perrier G

    更新日期:2003-07-03 00:00:00

  • Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks.

    abstract::Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca2+ signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and ro...


    pub_type: 杂志文章


    authors: Liu KH,Niu Y,Konishi M,Wu Y,Du H,Sun Chung H,Li L,Boudsocq M,McCormack M,Maekawa S,Ishida T,Zhang C,Shokat K,Yanagisawa S,Sheen J

    更新日期:2017-05-18 00:00:00

  • BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax.

    abstract::Bcl-2 was isolated from the t(14;18) chromosomal breakpoint in follicular B-cell lymphoma. Bcl-2 has the unique oncogenic role of extending cell survival by inhibiting a variety of apoptotic deaths. An emerging family of Bcl-2-related proteins share two highly conserved regions referred to here as Bcl-2 homology 1 and...


    pub_type: 杂志文章


    authors: Yin XM,Oltvai ZN,Korsmeyer SJ

    更新日期:1994-05-26 00:00:00

  • Actin-based motility of vaccinia virus.

    abstract::The role of the cytoskeleton during viral infection is poorly understood. Here we show, using a combination of mutant and drug studies, that the intracellular enveloped form of vaccinia virus is capable of inducing the formation of actin tails that are strikingly similar to those seen in Listeria, Shigella and Rickett...


    pub_type: 杂志文章


    authors: Cudmore S,Cossart P,Griffiths G,Way M

    更新日期:1995-12-07 00:00:00

  • Diabetes mellitus and genetically programmed defects in beta-cell function.

    abstract::The pathways that control insulin secretion and regulate pancreatic beta-cell mass are crucial in the development of diabetes mellitus. Maturity-onset diabetes of the young comprises a number of single-gene disorders affecting pancreatic beta-cell function, and the consequences of mutations in these genes are so serio...


    pub_type: 杂志文章,评审


    authors: Bell GI,Polonsky KS

    更新日期:2001-12-13 00:00:00

  • West Nile virus emergence and large-scale declines of North American bird populations.

    abstract::Emerging infectious diseases present a formidable challenge to the conservation of native species in the twenty-first century. Diseases caused by introduced pathogens have had large impacts on species abundances, including the American chestnut, Hawaiian bird species and many amphibians. Changes in host population siz...


    pub_type: 杂志文章


    authors: LaDeau SL,Kilpatrick AM,Marra PP

    更新日期:2007-06-07 00:00:00

  • Targeting of temperate phages drives loss of type I CRISPR-Cas systems.

    abstract::On infection of their host, temperate viruses that infect bacteria (bacteriophages; hereafter referred to as phages) enter either a lytic or a lysogenic cycle. The former results in lysis of bacterial cells and phage release (resulting in horizontal transmission), whereas lysogeny is characterized by the integration o...


    pub_type: 杂志文章


    authors: Rollie C,Chevallereau A,Watson BNJ,Chyou TY,Fradet O,McLeod I,Fineran PC,Brown CM,Gandon S,Westra ER

    更新日期:2020-02-01 00:00:00

  • Measurement of thermal contribution to photoreceptor sensitivity.

    abstract::Activation of a visual pigment molecule to initiate phototransduction requires a minimum energy, Ea, that need not be wholly derived from a photon, but may be supplemented by heat. Theory predicts that absorbance at very long wavelengths declines with the fraction of molecules that have a sufficient complement of ther...


    pub_type: 杂志文章


    authors: Koskelainen A,Ala-Laurila P,Fyhrquist N,Donner K

    更新日期:2000-01-13 00:00:00

  • Global warming and climate forcing by recent albedo changes on Mars.

    abstract::For hundreds of years, scientists have tracked the changing appearance of Mars, first by hand drawings and later by photographs. Because of this historical record, many classical albedo patterns have long been known to shift in appearance over time. Decadal variations of the martian surface albedo are generally attrib...


    pub_type: 杂志文章


    authors: Fenton LK,Geissler PE,Haberle RM

    更新日期:2007-04-05 00:00:00

  • Generation of prion transmission barriers by mutational control of amyloid conformations.

    abstract::Self-propagating beta-sheet-rich protein aggregates are implicated in a wide range of protein-misfolding phenomena, including amyloid diseases and prion-based inheritance. Two properties have emerged as common features of amyloids. Amyloid formation is ubiquitous: many unrelated proteins form such aggregates and even ...


    pub_type: 杂志文章


    authors: Chien P,DePace AH,Collins SR,Weissman JS

    更新日期:2003-08-21 00:00:00

  • An eIF-4A-like protein is a suppressor of an Escherichia coli mutant defective in 50S ribosomal subunit assembly.

    abstract::The assembly of ribosomes in bacterial cells is a complex process that remains poorly characterized. The in vitro assembly of active ribosomal subunits from purified RNA and protein components indicates that all of the information for proper assembly resides in the primary sequences of these macromolecules. On the oth...


    pub_type: 杂志文章


    authors: Nishi K,Morel-Deville F,Hershey JW,Leighton T,Schnier J

    更新日期:1988-12-01 00:00:00

  • Structure and function of the Salmonella Typhi chimaeric A(2)B(5) typhoid toxin.

    abstract::Salmonella enterica serovar Typhi (S. Typhi) differs from most other salmonellae in that it causes a life-threatening systemic infection known as typhoid fever. The molecular bases for its unique clinical presentation are unknown. Here we find that the systemic administration of typhoid toxin, a unique virulence facto...


    pub_type: 杂志文章


    authors: Song J,Gao X,Galán JE

    更新日期:2013-07-18 00:00:00

  • Isolation of rare circulating tumour cells in cancer patients by microchip technology.

    abstract::Viable tumour-derived epithelial cells (circulating tumour cells or CTCs) have been identified in peripheral blood from cancer patients and are probably the origin of intractable metastatic disease. Although extremely rare, CTCs represent a potential alternative to invasive biopsies as a source of tumour tissue for th...


    pub_type: 杂志文章


    authors: Nagrath S,Sequist LV,Maheswaran S,Bell DW,Irimia D,Ulkus L,Smith MR,Kwak EL,Digumarthy S,Muzikansky A,Ryan P,Balis UJ,Tompkins RG,Haber DA,Toner M

    更新日期:2007-12-20 00:00:00

  • Kondo resonance in a single-molecule transistor.

    abstract::When an individual molecule, nanocrystal, nanotube or lithographically defined quantum dot is attached to metallic electrodes via tunnel barriers, electron transport is dominated by single-electron charging and energy-level quantization. As the coupling to the electrodes increases, higher-order tunnelling and correlat...


    pub_type: 杂志文章


    authors: Liang W,Shores MP,Bockrath M,Long JR,Park H

    更新日期:2002-06-13 00:00:00

  • Strain effects and phase transitions in photonic resonator crystals.

    abstract::Optical structures with periodic variations of the dielectric constant in one or more directions (photonic crystals) have been employed extensively for studying optical diffraction phenomena. Practical interest in such structures arises from the possibilities they offer for tailoring photon modes, and thereby the char...


    pub_type: 杂志文章


    authors: Pier H,Kapon E,Moser M

    更新日期:2000-10-19 00:00:00

  • Controlled growth factor release from synthetic extracellular matrices.

    abstract::Polymeric matrices can be used to grow new tissues and organs, and the delivery of growth factors from these matrices is one method to regenerate tissues. A problem with engineering tissues that exist in a mechanically dynamic environment, such as bone, muscle and blood vessels, is that most drug delivery systems have...


    pub_type: 杂志文章


    authors: Lee KY,Peters MC,Anderson KW,Mooney DJ

    更新日期:2000-12-21 00:00:00

  • Gridlock signalling pathway fashions the first embryonic artery.

    abstract::Arteries and veins are morphologically, functionally and molecularly very different, but how this distinction is established during vasculogenesis is unknown. Here we show, by lineage tracking in zebrafish embryos, that angioblast precursors for the trunk artery and vein are spatially mixed in the lateral posterior me...


    pub_type: 杂志文章


    authors: Zhong TP,Childs S,Leu JP,Fishman MC

    更新日期:2001-11-08 00:00:00

  • Mechanistic studies of an unprecedented enzyme-catalysed 1,2-phosphono-migration reaction.

    abstract::(S)-2-hydroxypropylphosphonate ((S)-2-HPP) epoxidase (HppE) is a mononuclear non-haem-iron-dependent enzyme responsible for the final step in the biosynthesis of the clinically useful antibiotic fosfomycin. Enzymes of this class typically catalyse oxygenation reactions that proceed via the formation of substrate radic...


    pub_type: 杂志文章


    authors: Chang WC,Dey M,Liu P,Mansoorabadi SO,Moon SJ,Zhao ZK,Drennan CL,Liu HW

    更新日期:2013-04-04 00:00:00

  • Occluded bound calcium on the phosphorylated sarcoplasmic transport ATPase.

    abstract::The Ca2+ + Mg2+-activated ATPase of the sarcoplasmic reticulum is responsible for the active Ca2+ transport of this membrane system, the key feature of which is the formation of an energy-rich phosphorylated transport enzyme (EP) and its conversion. To understand the Ca2+-transport mechanism, it is essential to clarif...


    pub_type: 杂志文章


    authors: Takisawa H,Makinose M

    更新日期:1981-03-19 00:00:00