Abstract:
:It is well established that muscle contraction results from the relative sliding of actin and myosin filaments. Both filaments have definite polarities and well-ordered structures. Thick filaments, however, are not vital for supporting movement in vitro. Previously we have demonstrated that actin filaments can move continuously on myosin fragments (subfragment-1 or heavy meromyosin (HMM] that are bound to a nitrocellulose surface. Here we report that actin filaments can move in opposite directions on tracks of myosin heads formed when actin filaments decorated with HMM are placed on a nitrocellulose surface. The actin filaments always move forward, frequently changing the direction of the movement, but never move backward reversing the polarity of the movement. The direction of movement is therefore determined by the polarity of the actin filament. These results indicate that myosin heads have considerable flexibility.
journal_name
Naturejournal_title
Natureauthors
Toyoshima YY,Toyoshima C,Spudich JAdoi
10.1038/341154a0subject
Has Abstractpub_date
1989-09-14 00:00:00pages
154-6issue
6238eissn
0028-0836issn
1476-4687journal_volume
341pub_type
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