Abstract：
:AtT-20 cells are known to synthesize two molecular weight forms of the prohormone converting enzyme PC1 with molecular masses of 87 and 66 kDa. In this study we have analyzed basal and stimulated secretion of these proteins. Western blot results show that basal secretion medium of cultured AtT-20 cells contained low concentrations of both the 87 and 66 kDa forms of PC1 with the former protein predominant. During the stimulation period with CRF, cAMP and cAMP + BaCl2, increased release of both proteins was observed, but the 66 kDa protein predominated. Secretion medium obtained from stimulated and unstimulated cells was enzymatically active against the Cbz-Arg-Ser-Lys-Arg-AMC fluorogenic substrate as well as against 35S-proenkephalin. This activity was Ca+2 dependent and was inhibited by the chelating agent EDTA. The activity was insensitive to acid and thiol proteinase inhibitors as well as to N-alpha-p-tosyl-L-Lys-chloromethyl ketone; it was slightly sensitive to phenylmethyl sulfonyl fluoride and was strongly inhibited by D-Tyr-Ala-Lys-Arg-chloromethyl ketone. This inhibitor profile exhibits strong similarities to furin and kexin. After partial purification of medium by gel filtration chromatography, a portion of the enzymatic activity and immunoreactivity for both 87 kDa and 66 kDa proteins eluted with an apparent molecular weight of 400 kDa (suggesting aggregation); however the highest activity appeared in the elution position of the 66 kDa monomer. When the 87 kDa protein was removed from the medium by means of an affinity column containing an antibody against the carboxyl terminal portion of PC1, the column flow-through, which included the 66 kDa protein, still remained enzymatically active. These data support the notion that the 66 kDa protein, which is the most concentrated PC1 product stored in AtT-20 cells and is released during stimulation, is enzymatically active.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Vindrola O,Lindberg Idoi
10.1016/0143-4179(93)90096-ssubject
Has Abstractpub_date
1993-08-01 00:00:00pages
151-60issue
2eissn
0143-4179issn
1532-2785pii
0143-4179(93)90096-Sjournal_volume
25pub_type
杂志文章相关文献
NEUROPEPTIDES文献大全abstract：:The human placenta contains membrane bound opioid receptors. The binding of the antagonist naloxone and the fairly kappa-selective agonist EKC were studied in the microvillous membrane fraction. In both cases high affinity binding sites were detected with Kd values in the nanomolar range. A series of kappa-selective l...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(88)90050-9
更新日期：1988-10-01 00:00:00
abstract：:Hippocampal neurogenesis is important for modulating the behavioural responses to stress and for certain forms of learning and memory. The mechanisms underlying the necessary coupling of neuronal activity to neural stem/progenitor cell (NSPC) function remain poorly understood. Within the dentate subgranular stem cell ...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1016/j.npep.2013.10.002
更新日期：2013-12-01 00:00:00
abstract：:Oxytocin (OXT), a nonapeptide hormone of posterior pituitary, reaches the central nervous system from systemic blood circulation with a difficulty because of the blood-brain barrier (BBB). The interest has been expressed in the use of the nasal route for delivery of OXT to the brain directly, exploiting the olfactory ...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2012.12.003
更新日期：2013-04-01 00:00:00
abstract：:Possible positive reinforcing and aversive effects of intraperitoneally (ip) administered substance P (SP) and its C-terminal heptapeptide analog [pGlu5, MePhe8, Sar9]-SP5-11 (DIME-C7) were investigated in rats. The 'conditioned corral preference and avoidance procedure' was used for assessing positive and negative re...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(90)90037-y
更新日期：1990-12-01 00:00:00
abstract：:The effect of the selective non-peptide antagonist for NK1 receptors (+/-)CP 96,345 on cellular transduction mechanisms elicited by the NK1 selective agonist [Sar9]-substance P-sulfone ([Sar9]-SP) was investigated in a stabilized culture of human skin fibroblasts (HF) and compared to the effects of two peptide antagon...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/s0143-4179(96)90023-2
更新日期：1996-08-01 00:00:00
abstract：:Rat/mouse hemokinin-1 (r/m HK-1) has been identified as a member of the tachykinin family and its effect in colonic contractile activity remains unknown. We investigated the effects and mechanisms of actions of r/m HK-1 on the mouse colonic contractile activity in vitro by comparing it with that of substance P (SP). R...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2009.03.004
更新日期：2009-06-01 00:00:00
abstract：:Galanin, a 29 residue peptide found in the hypothalamus, causes orexigenic effects in a variety of species. In the present study, we investigated appetite-associated effects of galanin in chicks from lines which have been selected from a common founder population for either low or high body weight. The low line consis...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2012.11.003
更新日期：2013-08-01 00:00:00
abstract：:We have developed an extraction method and radioimmunoassay for the measurement of interleukin-1 beta (IL-1 beta) in rat tissues, using an antibody specific for IL-1 beta, IL-1 beta was detected in the spleen and thymus of control rats. Adrenalectomy (ADX) had no effect upon control levels of IL-1 beta. In animals wit...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(93)90008-x
更新日期：1993-06-01 00:00:00
abstract：:Endomorphins are newly discovered mu-opioid receptor selective immunocompetent opioid peptides. Endomorphin 1 is predominantly distributed in brain, while endomorphin 2 is widely allocated in the spinal cord. Lately, endomorphins have been investigated as modulators of reactive oxygen and nitrogen species. Nitric oxid...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2009.11.004
更新日期：2010-02-01 00:00:00
abstract：:Microchemotaxis chambers were used to investigate whether one aspect of ciliary neurotrophic factor CNTF's role as a lesion factor might be to promote the initial early recruitment of macrophages, which express the signal transducing receptor components, gp130 and LIFRbeta. CNTFRalpha alone, or in combination with CNT...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1054/npep.2000.0829
更新日期：2000-12-01 00:00:00
abstract：:Glucocorticoid deficiency is associated with a decrease of food intake. Orexigenic peptides, neuropeptide Y (NPY) and agouti related protein (AgRP), and the anorexigenic peptide proopiomelanocortin (POMC), expressed in the arcuate nucleus of the hypothalamus (ARC), are regulated by meal-induced signals. Orexigenic neu...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2012.02.002
更新日期：2012-06-01 00:00:00
abstract：:Pheochromocytoma (PC-12) cells exposed to nerve growth factor (NGF) acquire a sympathetic neuron-like phenotype. This NGF-response is blocked by methylation inhibitors and can be mimicked by the farnesylated methylated small GTP-binding protein p21ras. The implicated involvement of prenylation, methylation and a small...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(93)90036-a
更新日期：1993-01-01 00:00:00
abstract：:Attempts have been made to confirm reports that naloxonazine and Mr2034 are able to selectively antagonise morphine-induced analgesia and respiratory depression respectively. In the present studies these antagonists proved to be equi-effective against both the analgesic and respiratory depressant effects of morphine, ...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(84)90033-7
更新日期：1984-12-01 00:00:00
abstract：:Several lines of evidence indicate a role for neuropeptide Y (NPY) in the modulation of the corticotroph axis. In two separate studies reported here, the concentrations of NPY and noradrenaline (NA), as well as corticotropin-releasing factor (CRF) and arginine vasopressin (AVP), were measured in extracts of individual...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(93)90107-l
更新日期：1993-10-01 00:00:00
abstract：:The biological and immunological properties of insulin-like material produced by brain cells from 12 day old mice, cultured in serum-free media were examined. Differences were seen in the rate of appearance, relative amounts and behaviour on HPLC between immunoreactive and biologically active insulin-like material. Ex...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(87)90006-0
更新日期：1987-05-01 00:00:00
abstract：:Lumbar disc herniation is a common cause of radicular pain, but the mechanism remains ambiguous and the treatment stays unsatisfied. Many studies revealed a traditional Chinese medicine puerarin may moderate chronic pain from diabetes and nerve injury. Thus far, the role and mechanism of puerarin in radicular pain is ...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2018.10.001
更新日期：2018-12-01 00:00:00
abstract：:Recent studies have shown that oxytocin reduces food intake and body weight gain and promotes lipolysis in some species, including humans. Interestingly, these effects of oxytocin are more marked in obese individuals. Although the menopausal loss of ovarian function induces increased visceral adiposity and some metabo...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2019.03.002
更新日期：2019-06-01 00:00:00
abstract：:In an attempt to determine the localization of the messenger RNA (mRNA) encoding somatostatin in the rat retina, we studied Sprague-Dawley rats by in situ hybridization histochemistry using radiolabelled oligodeoxyribonucleotides complementary for rat somatostatin mRNA. Among the layers of retina, we found specific la...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(90)90135-l
更新日期：1990-09-01 00:00:00
abstract：:We have previously shown that the kappa-opioid agonist, asimadoline, produces time-dependent changes in neuropeptide concentrations in the joints of rats with chronic arthritis. We hypothesized that asimadoline acts on peripheral terminals to modulate substance P (SP) release. To address this hypothesis, here we have ...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1054/npep.2000.0813
更新日期：2000-06-01 00:00:00
abstract：:Here we report that chicken pineal membranes express high affinity receptors for Vasoactive Intestinal Peptide (VIP), as revealed by competitive displacement analysis of [3-iodotyrosyl-125I]-VIP by native VIP. These binding sites were further characterized by covalent cross-linking of radioiodinated VIP to chicken pin...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(91)90066-r
更新日期：1991-05-01 00:00:00
abstract：:Omega Conotoxin is a peptide venom of the marine snail Conus geographus which has been postulated as a neural specific calcium channel blocker in several systems. In the present study in rat ileum strips omega-conotoxin GVIA specifically blocked neuronal responses evoked by electrical field stimulation in a dose-depen...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(89)90078-4
更新日期：1989-05-01 00:00:00
abstract：:Dermorphin (Tyr-D-Ala-Phe-Gly-Tyr-Pro-Ser-NH2), a naturally occurring peptide isolated from arboreal frog skin, is endowed with outstanding structural and biological features. It has no structural community with the sequence of mammalian opioid peptides and is a unique example of a D-aminoacid containing peptide which...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(89)90021-8
更新日期：1989-01-01 00:00:00
abstract：:Nesfatin-1, a newly discovered satiety peptide, has recently been reported to be involved in the stress response. Stress-induced expression of nesfatin-1 has been reported and few studies focus on its expression in the hypothalamus, which is the center of the stress response. To test our hypothesis that peripheral and...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2015.08.003
更新日期：2015-12-01 00:00:00
abstract：:DPDPE ([D-Pen2, D-Pen5]-Enkephalin) and DPLPE ([D-Pen2, L-Pen5]-Enkephalin) are conformationally-constrained cyclic analogs of enkephalin with high selectivity for delta opioid receptors. Intracerebroventricular (i.c.v.) administration of each analog acutely produces a complex EEG response in rats characterized by a d...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(84)90065-9
更新日期：1984-12-01 00:00:00
abstract：:Nociceptin/Orphanin FQ (N/OFQ) is a 17-amino acid peptide that binds to the nociceptin receptor (NOP). N/OFQ and NOP receptors are expressed in numerous brain areas. The generation of specific agonists, antagonists and receptor-deficient mice or rats has enabled progress in elucidating the biological functions of N/OF...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1016/j.npep.2015.03.005
更新日期：2015-04-01 00:00:00
abstract：:Since there are conflicting reports regarding the effects of somatostatin (SS) on cyclic AMP levels in astrocytes derived from rat cerebral cortex and, to date, the SS binding to mature astrocytes is unknown, the present study has determined SS binding and its effect on cyclic AMP accumulation in a fresh astrocyte-ric...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(92)90002-e
更新日期：1992-09-01 00:00:00
abstract：:We have assessed the influence of acute bilateral nephrectomy, of captopril and saralasin, on the hypotensive activity of neurotensin (NT) and of various hypotensive drugs in pentobarbital-anesthetized rats. The results show that the hypotensive activity of NT and of compound 48/80 (C48/80), in contrast to that of his...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(83)90047-1
更新日期：1983-04-01 00:00:00
abstract：:Neuropeptide Y (NPY) neurons abundantly innervate the hypothalamus, where NPY is involved in the regulation and integration of a broad range of homeostatic functions. In order to understand NPY-mediated behavioral, autonomic and neuroendocrine effects, it is important to characterize in detail the distribution of the ...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1016/j.npep.2004.05.009
更新日期：2004-08-01 00:00:00
abstract：:The progesterone metabolite, allopregnanolone (AlloP), is a GABAA receptor modulating steroid and is known to have orexigenic and pro-obesity effects. The neurobiological mechanisms underpinning these effects are most likely due to enhanced GABAergic signaling in the lateral arcuate nucleus (ARC) and medial paraventri...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2019.101937
更新日期：2019-08-01 00:00:00
abstract：:Prader-Willi syndrome (PWS) is the predominant genetic cause of obesity in humans and is associated with several behavioural phenotypes such as altered motoric function, reduced activity, and learning disabilities. It can include mood instability and, in some cases, psychotic episodes. Recently, the Snord116 gene has ...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2015.06.009
更新日期：2015-10-01 00:00:00
