Abstract：
:The biological and immunological properties of insulin-like material produced by brain cells from 12 day old mice, cultured in serum-free media were examined. Differences were seen in the rate of appearance, relative amounts and behaviour on HPLC between immunoreactive and biologically active insulin-like material. Excess anti-insulin sera failed to suppress most of the biological activity and the culture medium was shown to contain relatively large quantities of immunoreactive IGF-I (590 ng/30 ml). The elution profile of this material on HPLC overlapped but was not identical with the insulin biological activity, which indicated that other insulin-like growth factors were also present. The relatively high concentration of IGF-I produced by the brain cell cultures suggest that this preparation may be suitable for biosynthetic studies of this growth factor.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Birch NP,Christie DL,Renwick AGdoi
10.1016/0143-4179(87)90006-0subject
Has Abstractpub_date
1987-05-01 00:00:00pages
325-31issue
4eissn
0143-4179issn
1532-2785pii
0143-4179(87)90006-0journal_volume
9pub_type
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