Abstract:
:Six of the genes encoding enzymes of the beta-ketoadipate pathway for benzoate and 4-hydroxybenzoate degradation in Pseudomonas putida are organized into at least three separate transcriptional units. As an initial step to defining this pca regulon at the molecular level, lacZ fusions were made with the pcaI and pcaJ genes, which encode the two subunits of beta-ketoadipate:succinyl-coenzyme A transferase, the enzyme catalyzing the next-to-last step in the beta-ketoadipate pathway. Fusion analyses showed that pcaI and pcaJ constitute an operon which requires beta-ketoadipate or its nonmetabolizable analog, adipate, as well as the pcaR regulatory gene for induction. The pcaIJ promoter is likely to be a sigma 70-type promoter; it has a sigma 70-type consensus sequence and did not require the alternative sigma factor, RpoN, for induction. Deletion analysis of the promoter region of a pcaI-lacZ transcriptional fusion indicated that no specific DNA sequences upstream of the -35 region were required for full induction. This implies that the binding site for the activator protein, PcaR, is unusually close to the transcriptional start site of pcaIJ.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Parales RE,Harwood CSdoi
10.1128/jb.175.18.5829-5838.1993subject
Has Abstractpub_date
1993-09-01 00:00:00pages
5829-38issue
18eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract::Remsen, C. C. (Syracuse University, Syracuse, N.Y.), D. G. Lundgren, and R. A. Slepecky. Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol. J. Bacteriol. 91:324-331. 1966.-The effect of phenethyl alcohol (PEA) upon the initial stages of sporulation in Bacillus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.324-331.1966
更新日期:1966-01-01 00:00:00
abstract::The bacterial second messenger cyclic di-GMP (c-di-GMP) regulates the transition between sessility and motility. In Salmonella enterica serovar Typhimurium, the expression of CsgD, the regulator of multicellular rdar morphotype behavior, is a major target of c-di-GMP signaling. CsgD expression is positively regulated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00290-09
更新日期:2009-06-01 00:00:00
abstract::Deletion mutagenesis analysis of a duplicated gene necessary for Haemophilus influenzae serotype b capsule expression showed that only one functional copy of this gene is required for capsule production and for virulence in infant rats. Mutant strains generated in this study differed from each other and from the paren...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.44-48.1986
更新日期:1986-07-01 00:00:00
abstract::IS630 is a 1.15-kilobase sequence in Shigella sonnei that, unlike many mobile elements, seems not to mediate cointegration between different replicons. To assess its transposition, we constructed composite elements containing inverted copies of IS630 flanking a drug resistance gene. We found that these composite eleme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3830-3836.1990
更新日期:1990-07-01 00:00:00
abstract::Growth of Schizosaccharomyces pombe in batch cultures in a commercial defined medium was found to be normal until the last generation before the stationary phase. Approximately one-half the progeny of the last division lysed. Lysis occurred at breaks in the cell wall at cell-plates whose fission was eccentric. Normal ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.192-195.1967
更新日期:1967-07-01 00:00:00
abstract::Chloromuconate cycloisomerases of bacteria utilizing chloroaromatic compounds are known to convert 3-chloro-cis,cis-muconate to cis-dienelactone (cis-4-carboxymethylenebut-2-en-4-olide), while usual muconate cycloisomerases transform the same substrate to the bacteriotoxic protoanemonin. Formation of protoanemonin req...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.15.4551-4561.2001
更新日期:2001-08-01 00:00:00
abstract::XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to clon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.4183-4186.1995
更新日期:1995-07-01 00:00:00
abstract::Investigation of the chromosomal region downstream of the lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus revealed the presence of a gene (prtB) encoding a proteinase of 1,946 residues with a predicted molecular mass of 212 kDa. The deduced amino acid sequence showed that PrtB proteinase displays significan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3059-3065.1996
更新日期:1996-06-01 00:00:00
abstract::Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00237-11
更新日期:2011-05-01 00:00:00
abstract::Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1803-1810.1968
更新日期:1968-11-01 00:00:00
abstract::Agrobacterium tumefaciens transfers single-stranded DNAs (T strands) into plant cells. VirE1 and VirE2, which is a single-stranded DNA binding protein, are important for tumorigenesis. We show that T strands and VirE2 can enter plant cells independently and that export of VirE2, but not of T strands, depends on VirE1....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.4.1207-1212.1996
更新日期:1996-02-01 00:00:00
abstract::Hasenclever, H. F. (U. S. Public Health Service, Bethesda, Md.), William O. Mitchell, and Joseph Loewe. Antigenic studies of Candida. II. Antigenic relation of Candida albicans group A and group B to Candida stellatoidea and Candida tropicalis. J. Bacteriol. 82:574-577. 1961.-Previous work in our laboratory has shown ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.4.574-577.1961
更新日期:1961-10-01 00:00:00
abstract::The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.361-364.1970
更新日期:1970-02-01 00:00:00
abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00373-15
更新日期:2016-01-11 00:00:00
abstract::The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3783-3785.1997
更新日期:1997-06-01 00:00:00
abstract::Strain DCB-1 is a strict anaerobe capable of reductive dehalogenation. We elucidated metabolic processes in DCB-1 which may be related to dehalogenation and which further characterize the organism physiologically. Sulfoxy anions and CO2 were used by DCB-1 as catabolic electron acceptors. With suitable electron donors,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.2065-2070.1990
更新日期:1990-04-01 00:00:00
abstract::A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The alpha-hemol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.525-530.1967
更新日期:1967-02-01 00:00:00
abstract::CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4766-4769.1994
更新日期:1994-08-01 00:00:00
abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
更新日期:1990-07-01 00:00:00
abstract::ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01606-07
更新日期:2009-03-01 00:00:00
abstract::In response to iron limitation, the siderophore enterobactin is synthesized and secreted by Escherichia coli. Its biosynthesis is performed by a series of enzymes encoded by the Ent gene cluster. Among the genes of this cluster, ybdB has not been implicated in enterobactin production to date. We demonstrate here an in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00755-07
更新日期:2007-10-01 00:00:00
abstract::Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.) and Guy P. Youmans. Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis. J. Bacteriol. 84:708-715. 1962.-A substance, called mycosuppressin, was found in the lungs of guinea pigs and rabbits vaccinated with BCG or...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.708-715.1962
更新日期:1962-10-01 00:00:00
abstract::RSF1010 plasmid DNA was introduced into Pseudomonas putida and P. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in Pseudomonas species. The number of copies of RSF1010 was 43 per chromosome equivalent in P. putida cells. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1527-1529.1978
更新日期:1978-03-01 00:00:00
abstract::From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.1090-1094.1980
更新日期:1980-08-01 00:00:00
abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00799-16
更新日期:2017-05-09 00:00:00
abstract::The penicillin-binding proteins (PBPs) of 209 cell division (or growth) temperature-sensitive mutants of Streptococcus faecium were analyzed in this study. A total of nine strains showed either constitutive or temperature-sensitive conditional damage in the PBPs. Analysis of these nine strains yielded the following re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2432-2439.1987
更新日期:1987-06-01 00:00:00
abstract::The biochemical basis for the acquired or natural resistance of various gram-positive organisms to glycopeptides was studied by high-pressure liquid chromatographic analysis of their peptidoglycan UDP-MurNAc-peptide precursors. In all cases, resistance was correlated with partial or complete replacement of the C-termi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.8.2398-2405.1994
更新日期:1994-04-01 00:00:00
abstract::The pyrimidine de novo biosynthesis pathway has been characterized for a number of organisms. The general pathway consists of six enzymatic steps. In the characterization of the pyrimidine pathway of Lactococcus lactis, two different pyrD genes encoding dihydroorotate dehydrogenase were isolated. The nucleotide sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.3975-3982.1994
更新日期:1994-07-01 00:00:00
abstract::Drugs known to interrupt the chain of protein synthesis (streptomycin, chloramphenicol, ethidium bromide) appeared to affect production of the group D lysin by Streptococcus zymogenes by inhibiting growth. Drugs which block cell wall synthesis (vancomycin, bacitracin, d-cycloserine, and phosphonomycin) inhibited lytic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.2.844-848.1971
更新日期:1971-11-01 00:00:00
abstract::Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2427-2430.1996
更新日期:1996-04-01 00:00:00