Abstract：
:Relaxin is a peptide hormone which consists of two polypeptide chains that are synthesized as a B-chain/C-peptide/A-chain precursor. We have used the polymerase chain reaction (PCR) to isolate and clone a relaxin-like cDNA from sheep placental RNA. This cDNA and two sheep genomic clones were characterised by nucleotide sequencing. A comparison of the sheep nucleotide sequence with exon II of pig relaxin revealed homology of 72%. The sheep sequence had numerous stop codons in the region corresponding to the C-peptide. Therefore, there is no open reading frame which would include the C-peptide and A-chain regions. Analysis of several animals indicates that the stop codons are not due to an allelic polymorphism and Southern blot analysis of genomic DNA reveals the presence of a single copy gene. The 5' RACE PCR protocol was used to obtain sequence information for the 5' relaxin-like RNA. This analysis reveals that unprocessed precursor RNA is the predominant RNA species in placenta. A small proportion of clones was isolated which contained novel 5' sequences. These sequences mostly appear to be generated from repetitive DNA elements upstream of exon II. No relaxin-like exon I sequence which encodes the B-chain was found after an extensive search of the 5' RACE PCR products. Therefore, this relaxin-like gene does not produce an RNA species in ovary, placenta or endometrial tissue which could give rise to a functional sheep relaxin hormone.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Roche PJ,Crawford RJ,Tregear GWdoi
10.1016/0303-7207(93)90250-nsubject
Has Abstractpub_date
1993-02-01 00:00:00pages
21-8issue
1-2eissn
0303-7207issn
1872-8057pii
0303-7207(93)90250-Njournal_volume
91pub_type
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更新日期：1991-08-01 00:00:00
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更新日期：2014-02-15 00:00:00
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更新日期：1992-11-01 00:00:00
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更新日期：1997-12-12 00:00:00
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更新日期：1998-05-25 00:00:00
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pub_type： 杂志文章,评审
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更新日期：1991-05-01 00:00:00
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更新日期：2018-01-15 00:00:00
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更新日期：2008-11-25 00:00:00
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pub_type： 杂志文章
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更新日期：1979-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-12-05 00:00:00
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更新日期：2014-05-05 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular and cellular endocrinology
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更新日期：2013-05-22 00:00:00
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