当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Repression of sterol regulatory element-binding protein 1-c is involved in the protective effects of exendin-4 in pancreatic β-cell line.

Repression of sterol regulatory element-binding protein 1-c is involved in the protective effects of exendin-4 in pancreatic β-cell line.

Abstract：

:Exendin-4 (Ex-4), a long-acting agonist of glucagon-like peptide-1 receptor, is a novel anti-diabetic drug that prevents β-cells against various toxicities. However, the mechanism and molecules mediating the protection procession of Ex-4 are not fully understood. We investigated the protective effect of Ex-4 against lipotoxicity, mediated by a repression of sterol regulatory element-binding protein (SREBP)-1c, a regulator of genes expression involved in fat and cholesterol synthesis. To observe the effect of Ex-4, we evaluated glucose-stimulated insulin secretion (GSIS) and apoptosis in the MIN6 pancreatic β-cell line, which were cultured in DMEM medium containing 500 μM palmitate, with or without 10 nM Ex-4. We also examined the roles of SREBP-1c in lipotoxicity model by knockdown with si-RNA. Treatment with Ex-4 improved insulin secretion and survival as well as reduced SREBP-1c expression and activity in palmitate-treated MIN6 cells. This improvement was accompanied with an upregulation of PI3K/Akt signaling pathway, and LY294.002, a specific inhibitor of PI3 kinase, abrogated effects of Ex-4 on insulin secretion. Moreover, SREBP-1c in nuclei was increased by the inhibition of PI3 kinase. Lipotoxic effects of palmitate in the insulin secretion and apoptosis were significantly prevented by SREBP-1 knockdown. In conclusion, Ex-4 protects β-cell against palmitate-induced β-cell dysfunction and apoptosis, by inhibiting SREBP-1c expression and activity through the PI3K/Akt signaling pathway.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Hong SW,Lee J,Park SE,Rhee EJ,Park CY,Oh KW,Park SW,Lee WY

doi

10.1016/j.mce.2012.07.004

subject

Has Abstract

pub_date

2012-10-15 00:00:00

pages

242-52

issue

1-2

eissn

0303-7207

issn

1872-8057

pii

S0303-7207(12)00355-3

journal_volume

362

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Role of hormone cofactors in the human papillomavirus-induced carcinogenesis of the uterine cervix.

    abstract：:If human papillomavirus (HPV) is necessary for the development of (pre)neoplastic lesions of the uterine cervix, it is not sufficient. Among the cofactors involved in the malignant transformation of cells infected by HPV, sex hormones may facilitate the cervical carcinogenesis by different mechanisms, including the in...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2006.10.014

    authors： Delvenne P,Herman L,Kholod N,Caberg JH,Herfs M,Boniver J,Jacobs N,Hubert P

    更新日期：2007-01-29 00:00:00

  • Specificities in the synthesis of a cytoplasmic estrogen-induced uterine protein.

    abstract：:The earliest known induction by estrogen of a specific uterine protein is that of the induced protein (IP) of Gorski and colleagues, which is demonstrable within 40 min after treatment. We found that this protein is not restricted to the rat; it was detected i the uterus of prepuberal C3H/eB and SWR mice, 1 h after i...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(76)90051-4

    authors： Sömjen D,Kaye AM

    更新日期：1976-05-01 00:00:00

  • Regulation of the biosynthesis of cytochromes P-450 involved in steroid hormone synthesis.

    abstract：:The actions of ACTH to regulate the synthesis of the various enzymes involved in steroid hormone biosynthesis have been studied using bovine adrenocortical cells in monolayer culture. ACTH causes an increase in the synthesis of both the mitochondrial and the microsomal forms of cytochrome P-450 involved in steroid hor...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/0303-7207(85)90123-6

    authors： Waterman MR,Simpson ER

    更新日期：1985-02-01 00:00:00

  • Impairment of learning and memory performances induced by BPA: Evidences from the literature of a MoA mediated through an ED.

    abstract：:Many rodent studies and a few non-human primate data report impairments of spatial and non-spatial memory induced by exposure to bisphenol A (BPA), which are associated with neural modifications, particularly in processes involved in synaptic plasticity. BPA-induced alterations involve disruption of the estrogenic pat...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2018.03.017

    authors： Mhaouty-Kodja S,Belzunces LP,Canivenc MC,Schroeder H,Chevrier C,Pasquier E

    更新日期：2018-11-05 00:00:00

  • Bradykinin stimulates prostaglandin E2 release in human skeletal muscular fibroblasts.

    abstract：:Local mediator prostaglandins and bradykinin are involved in inflammation and pain. We explored bradykinin effects on prostaglandin E2 (PGE2) release from fibroblasts derived from human skeletal muscular biopsies. Bradykinin induced PGE2 release through bradykinin B2 receptors, since PGE2 release was blocked by the br...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2020.110771

    authors： Muscella A,Cossa LG,Vetrugno C,Marsigliante S

    更新日期：2020-05-01 00:00:00

  • Asprosin impairs insulin secretion in response to glucose and viability through TLR4/JNK-mediated inflammation.

    abstract：:Severe inflammation in the islets is observed in obese patients with type 2 diabetes. Inflammation in the islets is caused by obesity-induced serum free fatty acids. Asprosin is a fasting-induced adipokine, which contributes to hepatic glucose production. However, the effects of asprosin on inflammation and cellular d...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.03.001

    authors： Lee T,Yun S,Jeong JH,Jung TW

    更新日期：2019-04-15 00:00:00

  • Role of adenine nucleotides in insulin secretion from MIN6 pseudoislets.

    abstract：:Insulin secretion from MIN6 cells configured as cell aggregates by culture on a gelatin substrate (pseudoislets) is enhanced compared to that of MIN6 cells grown as monolayers on tissue culture plastic, indicating the importance of beta-cell-to-beta-cell proximity for insulin release. In this study we have shown that ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(02)00051-5

    authors： Hauge-Evans AC,Squires PE,Belin VD,Roderigo-Milne H,Ramracheya RD,Persaud SJ,Jones PM

    更新日期：2002-06-14 00:00:00

  • Early environmental regulation of hippocampal glucocorticoid receptor gene expression: characterization of intracellular mediators and potential genomic target sites.

    abstract：:Environmental conditions in early life permanently alter the development of glucocorticoid receptor gene expression in the hippocampus and hypothalamic-pituitary-adrenal responses to acute or chronic stress. In part, these effects can involve an activation of ascending serotonergic pathways and subsequent changes in t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(01)00635-9

    authors： Weaver IC,La Plante P,Weaver S,Parent A,Sharma S,Diorio J,Chapman KE,Seckl JR,Szyf M,Meaney MJ

    更新日期：2001-12-20 00:00:00

  • The effect of nitric oxide donors on insulin secretion, cyclic GMP and cyclic AMP in rat islets of Langerhans and the insulin-secreting cell lines HIT-T15 and RINm5F.

    abstract：:The aim of this study was to investigate whether short-term treatment with nitric oxide donors could mimic cytokine inhibition of insulin secretion. We tested the nitric oxide generating compounds 3-morpholinosydnonimine (SIN-1), S-nitroso-N-penicillamine (SNAP), S-nitrosoglutathione and hydroxylamine for their abilit...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)90093-0

    authors： Cunningham JM,Mabley JG,Delaney CA,Green IC

    更新日期：1994-06-01 00:00:00

  • Differential expression and localisation of connexin-37 and connexin-43 in follicles of different stages in the 4-week-old mouse ovary.

    abstract：:Mammalian female fertility is critically dependent on timely coordinated intercellular communication between follicle cells. Gap junctions are small, membrane-bound, aqueous channels that couple adjacent cells ionically and gap junction proteins, connexin-37 (Cx37) and connexin-43 (Cx43) both participate in sustaining...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2004.10.014

    authors： Teilmann SC

    更新日期：2005-04-29 00:00:00

  • The expression of prostatic acid phosphatase is transcriptionally regulated in human prostate carcinoma cells.

    abstract：:The expression of prostatic acid phosphatase (PAcP) in three human prostate carcinoma cell lines including LNCaP, DU 145 and PC-3, was studied to explore its potential role as a marker in the progression of prostate cancer. Although Southern blot analysis suggested the presence of PAcP gene in all three prostate carci...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)03544-h

    authors： Garcia-Arenas R,Lin FF,Lin D,Jin LP,Shih CC,Chang C,Lin MF

    更新日期：1995-04-28 00:00:00

  • Wnt/β-catenin signaling regulates follicular development by modulating the expression of Foxo3a signaling components.

    abstract：:Wnt signaling is an evolutionarily conserved pathway that regulates cell proliferation, differentiation and apoptosis. To investigate the possible role of Wnt signaling in the regulation of ovarian follicular development, secondary follicles were isolated and cultured in vitro in the presence or absence of its activat...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2013.11.007

    authors： Li L,Ji SY,Yang JL,Li XX,Zhang J,Zhang Y,Hu ZY,Liu YX

    更新日期：2014-02-15 00:00:00

  • Evidence that corticotropin-releasing hormone inhibits cell growth of human breast cancer cells via the activation of CRH-R1 receptor subtype.

    abstract：:It has been previously shown that corticotropin-releasing hormone (CRH) exerts antiproliferative activity on an estrogen-dependent tumor cell line, i.e. human endometrial adenocarcinoma Ishikawa (IK) cells. Here we have investigated the effects of CRH on another estrogen-dependent tumor cell line, human breast cancer ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2006.10.006

    authors： Graziani G,Tentori L,Muzi A,Vergati M,Tringali G,Pozzoli G,Navarra P

    更新日期：2007-01-29 00:00:00

  • Immunological, paracrine and endocrine aspects of testicular immune privilege.

    abstract：:Protection of the spermatogenic cells from the host immune response is fundamental to male fertility. Significantly, this protection extends to the tolerance of foreign tissue grafts placed within the testicular environment, a phenomenon that is called 'immune privilege'. This privilege of the testis appears to involv...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2010.03.022

    authors： Meinhardt A,Hedger MP

    更新日期：2011-03-15 00:00:00

  • The role of the renin-angiotensin system in the development of insulin resistance in skeletal muscle.

    abstract：:The canonical renin-angiotensin system (RAS) involves the initial action of renin to cleave angiotensinogen to angiotensin I (ANG I), which is then converted to ANG II by the angiotensin converting enzyme (ACE). ANG II plays a critical role in numerous physiological functions, and RAS overactivity underlies many condi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2012.04.011

    authors： Henriksen EJ,Prasannarong M

    更新日期：2013-09-25 00:00:00

  • Characterization of the testicular, epididymal and endocrine phenotypes in the Leuven Vdr-deficient mouse model: targeting estrogen signalling.

    abstract：:Vitamin D is a key factor for calcium and bone homeostasis, but signalling through the vitamin D receptor (VDR) seems also to be important for testicular function. To test the functional role of vitamin D signalling we examined the male reproductive system of the Leuven Vdr-ablated (Vdr(-/-)) mice, previously establis...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2013.06.036

    authors： Blomberg Jensen M,Lieben L,Nielsen JE,Willems A,Jørgensen A,Juul A,Toppari J,Carmeliet G,Rajpert-De Meyts E

    更新日期：2013-09-05 00:00:00

  • Localization of the heparin binding site of follistatin.

    abstract：:To define the heparin-binding site of follistatin, the reduced and S-carboxymethylated recombinant human follistatin containing 288 amino acids was digested by Staphylococcus aureus V8. The digested product was subjected to sulfate cellufine column chromatography and the adsorbed peptide fragments eluted with a stepwi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(92)90094-m

    authors： Inouye S,Ling N,Shimasaki S

    更新日期：1992-12-01 00:00:00

  • Characterization of chromatin binding sites for different forms of uterine progesterone receptor.

    abstract：:We tested hamster uterine progesterone receptor (Rp) forms for binding to different chromatin preparations. Similar forms of chick oviduct Rp were used for comparison. Hamster Rp elutes from DEAE-Sephacel in the two peaks, peak I at 115 mM KCl and peak II at 205 mM KCl. Chick Rp peaks I and II elute at 125 mM and 300 ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(87)90096-7

    authors： Cobb AD,Leavitt WW

    更新日期：1987-07-01 00:00:00

  • Regulation of c-fos expression by IGF-I in bovine chromaffin cells: desensitization following cholinergic activation.

    abstract：:Effects of insulin-like growth factor I on the expression of the immediate-early gene c-fos in adrenomedullary tissue were assessed in bovine chromaffin cells in primary culture. The expression of c-fos mRNA and FOS protein were studied by Northern blot and immunofluorescence microscopy. IGF-I induced c-fos mRNA expre...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)90205-4

    authors： Skar R,Larsen TH,Serck-Hanssen G

    更新日期：1994-12-01 00:00:00

  • Impairment of ATP-induced Ca2+ -signalling in human thyroid cancer cells.

    abstract：:Extracellular nucleotides like ATP that activate the Ca2+ -phosphatidylinositol (PI) signalling pathway have been suggested to participate in the regulation of normal human thyroid function. We examined, whether P2y-purinergic receptors are expressed on human thyroid cancer cells and whether post-receptor Ca2+ signall...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(97)00141-x

    authors： Schöfl C,Rössig L,Mader T,Börger J,Pötter E,von zur Mühlen A,Brabant G

    更新日期：1997-09-30 00:00:00

  • Analysis of differential expression and characterization of PIN in the gonads during sex reversal in the red-spotted grouper.

    abstract：:The red-spotted grouper, Epinephelus akaara, is a protogynous hermaphroditic fish that shows the characteristic of natural sex change. In this study, 2-year-old female groupers were successfully reversed to functional males by oral administration of 17alpha-methyltestosterone (MT) for 42 days. The protein inhibitor of...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2009.04.015

    authors： Li SW,Long ZF,Du J,Liu SG,Wen JJ

    更新日期：2009-10-15 00:00:00

  • Effects of spermatogenic cycle on Stem Leydig cell proliferation and differentiation.

    abstract：:We reported previously that stem Leydig cells (SLC) on the surfaces of rat testicular seminiferous tubules are able to differentiate into Leydig cells. The proliferation and differentiation of SLCs seem likely to be regulated by niche cells, including nearby germ and Sertoli cells. Due to the cyclical nature of sperma...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.11.007

    authors： Guan X,Chen F,Chen P,Zhao X,Mei H,Liu J,Lian Q,Zirkin BR,Chen H

    更新日期：2019-02-05 00:00:00

  • GH3 and RC-4BC cell lines are not suitable as in vitro models to study prolactin modulation and AHR responsiveness in rat pituitary.

    abstract：:Some environmental contaminants and pharmaceuticals increase the incidence of uterine tumors in toxicological studies with rats. These tumors can result from a hormonal imbalance due to rat-specific disrupted pituitary prolactin regulation, and are therefore of questionable relevance for humans. In this study we compa...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.110520

    authors： van den Brand AD,Rubinstein E,van den Berg M,van Duursen MBM

    更新日期：2019-10-01 00:00:00

  • Visualization of unoccupied and occupied gonadotropin-releasing hormone receptors in living cells.

    abstract：:Three chimeras of the rat GnRH receptor (rGnRHR) and an enhanced green fluorescent protein (GFP) were assessed to examine their suitability as probes of the receptor in transfected GH3 cells. Direct fusion of GFP to the N or C terminus of the rGnRHR abolished the receptor ligand binding affinity and the chimeric recep...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(98)00204-4

    authors： Lin X,Cornea A,Janovick JA,Conn PM

    更新日期：1998-11-25 00:00:00

  • Assessment of sperm cryodamage and strategies to improve outcome.

    abstract：:Sperm cryopreservation still represents a valuable clinical aid in the management of infertility. Its current principal indications include (1) donor sperm insemination; (2) freezing before cancer therapy to maintain reproductive capacity; (3) patient's convenience; and (4) because of the outstanding success with ICSI...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(00)00343-9

    authors： Oehninger S,Duru NK,Srisombut C,Morshedi M

    更新日期：2000-11-27 00:00:00

  • Luteinising hormones activate a factor(s) in testicular interstitial fluid which increases testicular vascular permeability.

    abstract：:The mechanism by which hCG increases the rat testicular vascular permeability was studied by injecting the testes with hCG (0.1-100 ng) together with testicular interstitial fluid (150 mul) or with the fluid alone (control) and measuring the uptake of i.v. injected [125I]hCG and the interstitial fluid volume in the te...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(86)90138-3

    authors： Veijola M,Rajaniemi H

    更新日期：1986-05-01 00:00:00

  • Transcriptional activation of Reg2 and Reg3β genes by glucocorticoids and interleukin-6 in pancreatic acinar and islet cells.

    abstract：:Reg family proteins are expressed in the pancreas and involved in pancreatitis and islet β-cell growth. In order to explore transcriptional control, we transfected luciferase reporter genes driven by Reg promoters into acinar AR42J and islet MIN6 cells. Dexamethasone (DEX) significantly increased the promoter expressi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2012.10.018

    authors： Luo C,Li B,Liu L,Yin HP,Wang M,Liu JL

    更新日期：2013-01-30 00:00:00

  • Muscarinic acetylcholine receptor subtypes in the rat seminal vesicle.

    abstract：:The aim of the present study was to identify the muscarinic acetylcholine receptor (mAChR) mRNA subtypes in the rat seminal vesicle. Furthermore, the mAChR subtypes involved in the contraction of the seminal vesicle were also explored. Reverse transcriptase-polymerase chain reaction (PCR) was performed and five PCR pr...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2006.01.004

    authors： Hamamura M,Maróstica E,de Avellar MC,Porto CS

    更新日期：2006-03-09 00:00:00

  • Plasminogen activator inhibitor-1 expression and secretion are stimulated by growth hormone and interleukin-6 in 3T3-L1 adipocytes.

    abstract：:Various adipocytokines have been described which influence insulin sensitivity and vascular function profoundly and might, therefore, potentially link obesity, insulin resistance, and atherosclerosis. Among those, plasminogen activator inhibitor (PAI)-1 is an adipose-secreted factor upregulated in obesity and insulin ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2006.04.027

    authors： Kralisch S,Klein J,Lossner U,Blüher M,Paschke R,Stumvoll M,Fasshauer M

    更新日期：2006-07-11 00:00:00

  • Gonadotropin-releasing hormone overcomes follicle-stimulating hormone's inhibition of insulin-like growth factor-5 synthesis and promotion of its degradation in rat granulosa cells.

    abstract：:The effect of a gonadotropin-releasing hormone-agonist (GnRH-a) on the synthesis of insulin-like growth factor-binding protein-5 (IGFBP-5), a physiological marker for atresia, was investigated. Granulosa cells obtained from diethylstilbestrol (DES)-treated immature female rats were cultured in serum-free medium for 72...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)03511-5

    authors： Onoda N,Li D,Mickey G,Erickson G,Shimasaki S

    更新日期：1995-04-28 00:00:00