Abstract：
:It has been previously shown that corticotropin-releasing hormone (CRH) exerts antiproliferative activity on an estrogen-dependent tumor cell line, i.e. human endometrial adenocarcinoma Ishikawa (IK) cells. Here we have investigated the effects of CRH on another estrogen-dependent tumor cell line, human breast cancer MCF7 cells. In this paradigm, CRH given at a fixed concentration of 100 nM significantly inhibited cell growth induced by 100 nM estradiol (E2) after 48 and 72 h of incubation. This effect was not associated with the induction of apoptosis. CRH inhibition of cell proliferation was counteracted in a concentration-dependent manner by the non-selective CRH receptor antagonist, astressin, as well as by a CRH-R1 selective receptor antagonist, antalarmin. RNase protection assays carried out on MCF7 under basal conditions showed that these cells express in a constitutive manner the CRH-R1 receptor subtype. We have also investigated the putative source of CRH acting on breast cancer cells; we found that MCF7 cells express CRH mRNA under basal conditions and secrete sizable amounts of immunoreactive CRH, which leads to postulate the existence of paracrine-autocrine inhibitory mechanism operated by CRH in breast cancer cells.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Graziani G,Tentori L,Muzi A,Vergati M,Tringali G,Pozzoli G,Navarra Pdoi
10.1016/j.mce.2006.10.006subject
Has Abstractpub_date
2007-01-29 00:00:00pages
44-9issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(06)00479-5journal_volume
264pub_type
杂志文章abstract：:The red-spotted grouper, Epinephelus akaara, is a protogynous hermaphroditic fish that shows the characteristic of natural sex change. In this study, 2-year-old female groupers were successfully reversed to functional males by oral administration of 17alpha-methyltestosterone (MT) for 42 days. The protein inhibitor of...
journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(98)00051-3
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：1982-09-01 00:00:00
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(82)90013-2
更新日期：1982-04-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2014.08.003
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pub_type： 杂志文章
doi：10.1016/j.mce.2020.111098
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(91)90192-u
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0303-7207(01)00689-x
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
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更新日期：2015-11-05 00:00:00
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journal_title：Molecular and cellular endocrinology
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更新日期：2011-07-04 00:00:00
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journal_title：Molecular and cellular endocrinology
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doi：10.1016/j.mce.2009.10.017
更新日期：2010-02-05 00:00:00
abstract：:The teleost brain is characterized by exceptionally high levels of aromatase, the enzyme that converts androgens into estrogens, and by its continuous growth throughout life. Gonadal estrogens have been implicated in sex differentiation and the control of reproduction in adult fish, but the role of neural estrogens du...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2004.01.006
更新日期：2004-04-30 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2010.01.032
更新日期：2010-05-14 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(93)90105-s
更新日期：1993-10-01 00:00:00
abstract：:The template for our understanding of the physiological role of the adrenal cortex was set by Hans Selye, who demonstrated its key involvement in the response to stress, of whatever origin, and who also introduced the terms glucocorticoid and mineralocorticoid. Despite this, from the late 1940s on there was certainly ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2008.09.008
更新日期：2009-03-05 00:00:00
abstract：:During development, the insulin-like growth factor I (IGF-I) gene is expressed in a tissue specific manner; however, the molecular mechanisms governing its developmental regulation remain poorly defined. To examine the hypothesis that expression of the growth hormone (GH) receptor accounts, in part, for the tissue spe...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(99)00045-3
更新日期：1999-06-25 00:00:00
abstract：:Aldosterone and cortisol are the major mineralocorticoid and glucocorticoid produced by the human adrenal. Circulating levels of angiotensin II and potassium control the adrenal production of aldosterone, while the production of cortisol is controlled mainly by adrenocorticotropin. The capacity of the adrenal cortex t...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0303-7207(99)00051-9
更新日期：1999-05-25 00:00:00
