Abstract:
:The aim of this study was to investigate whether short-term treatment with nitric oxide donors could mimic cytokine inhibition of insulin secretion. We tested the nitric oxide generating compounds 3-morpholinosydnonimine (SIN-1), S-nitroso-N-penicillamine (SNAP), S-nitrosoglutathione and hydroxylamine for their ability to inhibit insulin secretion, raise cyclic GMP and lower cyclic AMP levels in isolated rat islets of Langerhans and the insulin-secreting cell lines HIT-T15 and RINm5F. In islets, all nitric oxide donors inhibited glucose-induced insulin secretion and raised cyclic GMP levels. SIN-1 and S-nitrosoglutathione also reduced cyclic AMP, while SNAP and hydroxylamine had no effect. Insulin secretion in HIT-T15 cells was inhibited by SIN-1, SNAP and hydroxylamine and in RINm5F cells by hydroxylamine. Inhibition of HIT-T15 and RINm5F cell insulin secretion was not accompanied by an increase in cyclic GMP levels. The degree of inhibition of insulin secretion was unrelated to the extent of release of nitric oxide by the compounds as measured by nitrite and nitrate production. More effective inhibition by S-nitrosoglutathione and hydroxylamine versus SIN-1 and SNAP may be related to intracellular versus extracellular site of nitric oxide generation.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Cunningham JM,Mabley JG,Delaney CA,Green ICdoi
10.1016/0303-7207(94)90093-0subject
Has Abstractpub_date
1994-06-01 00:00:00pages
23-9issue
1-2eissn
0303-7207issn
1872-8057pii
0303-7207(94)90093-0journal_volume
102pub_type
杂志文章abstract::It has already been shown that Wistar rat thyroid (WRT) cells in low concentrations of calf serum (0.5%) are under the influence of both thyrotropin (TSH) and insulin as regards growth. The present data show that epidermal growth factor (EGF), in concentrations up to 10 micrograms/ml, is not able to modify DNA synthes...
journal_title:Molecular and cellular endocrinology
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更新日期:1989-08-01 00:00:00
abstract::The role of protein kinase C (PKC) isoforms (PKCs) in GnRH-stimulated MAPK [ERK1/2, JNK1/2 and p38) phosphorylation was examined in gonadotrope derived cells. GnRH induced a protracted activation of ERK1/2 and a slower and more transient activation of JNK1/2 and p38MAPK. Gonadotropes express conventional PKCα and PKCβ...
journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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doi:10.1016/j.mce.2008.09.008
更新日期:2009-03-05 00:00:00
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1999-12-20 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(79)90102-3
更新日期:1979-05-01 00:00:00
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1983-07-01 00:00:00
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journal_title:Molecular and cellular endocrinology
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更新日期:2017-01-05 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
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更新日期:2017-12-15 00:00:00
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更新日期:1992-01-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.mce.2010.03.019
更新日期:2010-09-15 00:00:00
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2011.10.027
更新日期:2012-02-26 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/s0303-7207(97)00110-x
更新日期:1997-08-08 00:00:00
abstract::In order to study the distribution of LH (HCG) receptors on luteal cells ferritin was coupled to ovine LH with glutaraldehyde and purified by gel chromatography. The conjugate (FELH) competed with 125I-hCG for binding to isolated luteal membranes and stimulated a dose-dependent release of progesterone (P) from isolate...
journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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abstract::Granulosa cell-derived inhibin A (a dimer of alpha- and beta A-subunits), activin A (a homodimer of beta A-subunits) and the activin-binding protein follistatin are important regulators of human ovarian steroidogenesis. We here studied how 8-bromo-cAMP (8br-cAMP), a protein kinase A activator, and 12-O-tetradecanoylph...
journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1979-05-01 00:00:00