Abstract:
:T3 effects on myosin heavy chain gene expression were analysed in muscles undergoing different fates during metamorphosis. Muscle fate was followed by somatic gene transfer of a constitutively expressed luciferase vector. Persistent expression was found in dorsal muscle which is remodelled during metamorphosis whilst the signal disappeared in apoptosing caudal muscle. RNAse protection assay was used to follow production of myosin heavy chain isoforms: two isoforms identified as embryonic (E3 and E19) and one adult form (A7). The effects of T3 treatment were followed over 120 h. During this time frame E3 and A7 expression patterns were found to be similar in both caudal and dorsal muscles. Most notably, at 48 h E3 expression was significantly down-regulated and production of A7 significantly upregulated in both caudal and dorsal muscle. Thus T3-induced transitions in muscle gene expression are independent of muscle fate during amphibian metamorphosis.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Sachs LM,Lebrun JJ,de Luze A,Kelly PA,Demeneix BAdoi
10.1016/s0303-7207(97)00110-xsubject
Has Abstractpub_date
1997-08-08 00:00:00pages
211-9issue
2eissn
0303-7207issn
1872-8057pii
S0303-7207(97)00110-Xjournal_volume
131pub_type
杂志文章abstract::A pair of glands (Y-organs) in crustaceans synthesize and secrete ecdysteroid hormones; the obligate precursor for synthesis is circulating cholesterol. Ecdysteroid output by the Y-organs is regulated negatively by an eyestalk neurosecretory peptide, molt-inhibiting hormone (MIH). The question was addressed, does MIH ...
journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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更新日期:1986-09-01 00:00:00
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更新日期:2013-04-10 00:00:00
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更新日期:2011-01-30 00:00:00
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更新日期:2018-01-15 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2004.02.015
更新日期:2004-10-15 00:00:00
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journal_title:Molecular and cellular endocrinology
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更新日期:2001-03-28 00:00:00
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journal_title:Molecular and cellular endocrinology
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更新日期:2017-11-05 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2003-02-28 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2013-07-15 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1998-08-25 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.mce.2011.08.030
更新日期:2011-12-05 00:00:00