Stimulation of the alpha-fetoprotein promoter by unliganded thyroid hormone receptor in association with protein deacetylation.

abstract：:This short review aims to assess the application of basic quality assurance (QA) principles in published thyroid hormone bioanalytical methods using mass spectrometry (MS). The use of tandem MS, in particular linked to liquid chromatography has become an essential bioanalytical tool for the thyroid hormone research co...

journal_title：Molecular and cellular endocrinology

authors： Richards K,Rijntjes E,Rathmann D,Köhrle J

更新日期：2017-12-15 00:00:00

abstract：:The effects of angiotensin II on cytosolic free Ca2+ ion concentrations ([Ca2+]i) were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized...

journal_title：Molecular and cellular endocrinology

authors： Flores JA,Veldhuis JD,Leong DA

更新日期：1991-10-01 00:00:00

abstract：:The number of adipocyte progenitors is determined early in foetal and neonatal development in a process which may be altered by gender and excess nutrient intake, and which in turn determines fat mass in adulthood and the risk of developing obesity. Here we investigate the hypothesis that excess nutrients, in this cas...

journal_title：Molecular and cellular endocrinology

authors： Petrighi Polidori G,Lomax MA,Docherty K

更新日期：2012-09-25 00:00:00

abstract：:The most potent, physiologic activator of proopiomelanocortin (POMC) gene transcription is corticotropin releasing hormone (CRH) and increased intracellular cAMP is critical for this effect. The 5'-flanking region of the murine POMC gene has several potential binding sites for regulatory proteins. To characterize the ...

journal_title：Molecular and cellular endocrinology

authors： Bishop JF,Mouradian MM

更新日期：1993-11-01 00:00:00

abstract：:Perfluorooctanoic acid (PFOA) is a persistent pollutant worldwide and even found in human cord blood and breast milk. Some animal studies have reported that PFOA causes developmental toxicity such as fetal weight loss, but the mechanism is still unclear. This study focused on developmental toxicity of PFOA, particular...

journal_title：Molecular and cellular endocrinology

authors： Suh CH,Cho NK,Lee CK,Lee CH,Kim DH,Kim JH,Son BC,Lee JT

更新日期：2011-04-30 00:00:00

abstract：:The expression of the three genes encoding the components C1, C2 and C3 of prostatic binding protein (PBP) is under androgen control and restricted to the rat ventral prostate. The SstI-PvuII fragment of the first intron of the C3(1) gene displays two binding sites for ubiquitous transcription factors and one for a ti...

journal_title：Molecular and cellular endocrinology

authors： Celis L,Claessens F,Peeters B,Heyns W,Verhoeven G,Rombauts W

更新日期：1993-08-01 00:00:00

abstract：:Airway smooth muscle (ASM) cell hyperplasia driven by persistent inflammation is a hallmark feature of remodeling in asthma. Sex steroid signaling in the lungs is of considerable interest, given epidemiological data showing more asthma in pre-menopausal women and aging men. Our previous studies demonstrated that estro...

journal_title：Molecular and cellular endocrinology

authors： Ambhore NS,Katragadda R,Raju Kalidhindi RS,Thompson MA,Pabelick CM,Prakash YS,Sathish V

更新日期：2018-11-15 00:00:00

abstract：:Relaxin is a peptide hormone which consists of two polypeptide chains that are synthesized as a B-chain/C-peptide/A-chain precursor. We have used the polymerase chain reaction (PCR) to isolate and clone a relaxin-like cDNA from sheep placental RNA. This cDNA and two sheep genomic clones were characterised by nucleotid...

journal_title：Molecular and cellular endocrinology

authors： Roche PJ,Crawford RJ,Tregear GW

更新日期：1993-02-01 00:00:00

abstract：:Protein phosphorylation-dephosphorylation appears to be an essential component in the regulation of many cellular processes by hormones and drugs. This concept has developed primarily from in vitro biochemical studies in which various purified proteins have been phosphorylated and dephosphorylated by distinct protein ...

journal_title：Molecular and cellular endocrinology

authors： Manning DR,DiSalvo J,Stull JT

更新日期：1980-07-01 00:00:00

abstract：:The template for our understanding of the physiological role of the adrenal cortex was set by Hans Selye, who demonstrated its key involvement in the response to stress, of whatever origin, and who also introduced the terms glucocorticoid and mineralocorticoid. Despite this, from the late 1940s on there was certainly ...

journal_title：Molecular and cellular endocrinology

authors： Vinson GP

更新日期：2009-03-05 00:00:00

abstract：:Chemotactic and mitogenic activities of granulosa cells in developing follicles were studied. Immature rats were subcutaneously injected with 20 IU of pregnant mare's serum gonadotrophin and killed at various intervals after injection. The ovaries were removed and granulosa cells were isolated and cultured in a serum-...

journal_title：Molecular and cellular endocrinology

authors： Lin MT,Wei SJ,Wing LY

更新日期：1992-03-01 00:00:00

abstract：:A critical regulatory component of the hypothalamus/pituitary/adrenal axis (HPA) in mammals, reptiles and birds, and in the hypothalamus/pituitary/interrenal (HPI) axis of amphibians and teleosts (modern bony fishes) is the strict ligand selectivity of the melanocortin-2 receptor (MC2R). Tetrapod and teleost MC2R orth...

journal_title：Molecular and cellular endocrinology

authors： Dores RM,Garcia Y

更新日期：2015-06-15 00:00:00

abstract：:The angiotensin II (AngII) type 1 (AT(1)) receptor is a seven-transmembrane G protein-coupled receptor, and is involved in regulating the physiological and pathological process of the cardiovascular system. Systemically and locally generated AngII has agonistic action on AT(1) receptor, but recent studies have demonst...

journal_title：Molecular and cellular endocrinology

authors： Akazawa H,Yasuda N,Komuro I

更新日期：2009-04-29 00:00:00

abstract：:125I-glucagon was directly cross-linked to its receptor in isolated adipocyte plasma membranes using a UV irradiation procedure. This investigation resulted in identification of an adipocyte glucagon receptor complex of 62 kDa, present both in white and brown adipose tissues. The specificity of labeling was shown by i...

journal_title：Molecular and cellular endocrinology

authors： Iwanij V,Amos TM,Billington CJ

更新日期：1994-05-01 00:00:00

abstract：:The effects of calcitonin (CT) on the cellular transport and accumulation of Ca were studied in bone cells grown in primary culture. The following sequence of events was detected: (1) After 1--2 h of incubation with CT the initial rate of Ca influx into bone cells transiently increased. A longer incubation with CT (3-...

journal_title：Molecular and cellular endocrinology

authors： Eilam Y,Szydel N,Harell A

更新日期：1980-06-01 00:00:00

abstract：:We hypothesized that DNA methylation is involved in human adrenal functional zonation. mRNAs expression and methylation pattern of RARB, NR4A1 and HSD3B2 genes in human adrenal tissues (HAT) and in pediatric virilizing adrenocortical tumors (VAT) were analyzed. For analysis of the results samples were divided into 3 a...

journal_title：Molecular and cellular endocrinology

authors： Baquedano MS,Perez Garrido N,Goñi J,Saraco N,Aliberti P,Berensztein E,Rivarola MA,Belgorosky A

更新日期：2017-02-05 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a main factor promoting neovascularization (angiogenesis) of solid tumours as prostate carcinoma. Hypoxia stimulates VEGF gene expression by activating the hypoxia-inducible factor-1 (HIF-1alpha). In the present study, the hypoxia-mimicking agent Ni(2+) induced vasoactive i...

journal_title：Molecular and cellular endocrinology

authors： Collado B,Sánchez-Chapado M,Prieto JC,Carmena MJ

更新日期：2006-04-25 00:00:00

abstract：:Rat thyroid tissue and cultured rat thyrocyte lines contain two thyroglobulin (Tg) mRNAs: a 9 kb rTg-1 mRNA encoding the 330, kDa Tg monomer and a recently described 0.95 kb rTg-2 mRNA. These transcripts have identical 5' coding sequences (641 nucleotides); however, the 3' end of rTg-2 is comprised of coding and non-c...

journal_title：Molecular and cellular endocrinology

authors： Graves PN,Davies TF

更新日期：1993-06-01 00:00:00

abstract：:Central leptin action requires PI3K activity to modulate glucose homeostasis and peripheral metabolism. However, the mechanism behind this phenomenon is not clearly understood. We hypothesize that hypothalamic PI3K activity is important for the modulation of the AMP-activated protein kinase (AMPK)/acetyl-CoA carboxyla...

journal_title：Molecular and cellular endocrinology

authors： Roman EA,Reis D,Romanatto T,Maimoni D,Ferreira EA,Santos GA,Torsoni AS,Velloso LA,Torsoni MA

更新日期：2010-01-15 00:00:00

abstract：:Conventional in vitro autoradiographical techniques have been used to screen a number of ovine peripheral tissues for the presence of specific 2-[125I]iodomelatonin binding sites. Intense specific labelling (defined as that displaced by 1 microM melatonin) was observed in the adrenal cortex, and less intense specific ...

journal_title：Molecular and cellular endocrinology

authors： Helliwell RJ,Howell HE,Lawson W,Barrett P,Morgan PJ

更新日期：1994-08-01 00:00:00

abstract：:The effects of the preovulatory luteinizing hormone (LH) surge on the ovulatory process are mediated by prostaglandins (PGs), the synthesis of which involves prostaglandin synthetase and cytosolic phospholipase A2 (cPLA2). In our previous study, we systematically investigated the function of prostaglandin endoperoxide...

journal_title：Molecular and cellular endocrinology

authors： Tang H,Wang L,Chen Y,He J,Qu L,Guo Y,Liu Y,Liu X,Lin H

更新日期：2018-09-15 00:00:00

abstract：:A new strain, named WRT cells, has been generated from primary cultures of rat thyroids. The primary culture was grown in Coon's modified Ham's F12 medium with 5% calf serum, insulin, hydrocortisone, transferrin, somatostatin, glycyl-L-histidyl-L-lysine and thyrotropin (TSH). On the basis of the following facts, the W...

journal_title：Molecular and cellular endocrinology

authors： Brandi ML,Rotella CM,Mavilia C,Franceschelli F,Tanini A,Toccafondi R

更新日期：1987-11-01 00:00:00

abstract：:Glucose transport activity ([3H]D-glucose uptake) in liver sinusoidal membrane vesicles (SMVs) from hyperthyroid rats was significantly higher than that from euthyroid controls (2.1-times increase in V(max) with K(m) unchanged at approximately 18 mM), associated with increased GLUT2 expression. In contrast, glucose tr...

journal_title：Molecular and cellular endocrinology

authors： Kemp HF,Hundal HS,Taylor PM

更新日期：1997-04-04 00:00:00

abstract：:Ca2+-calmodulin-dependent protein phosphatase activity is found in cytoskeletons of Y-1 mouse adrenal and bovine fasciculata cells. The activity is inhibited by three inhibitors of calmodulin (trifluoperazine, W-7 and pimozide) with EC50 in the low micromolar range. Protein phosphatase activity is inhibited by vanadat...

journal_title：Molecular and cellular endocrinology

authors： Papadopoulos V,Brown AS,Hall PF

更新日期：1989-05-01 00:00:00

abstract：:Several lines of evidence indicate that the number of GnRH receptors (GnRH-R) and therefore, gonadotrope responsiveness to GnRH, is highly dependent upon the level of GnRH-R mRNA. To explore this aspect of regulation, we have isolated a 3.3 kb fragment encompassing the promoter region of the rat GnRH-R gene. Primer ex...

journal_title：Molecular and cellular endocrinology

authors： Pincas H,Forraï Z,Chauvin S,Laverrière JN,Counis R

更新日期：1998-09-25 00:00:00

abstract：:Mutations in FGFR1, GNRHR, PROK2, PROKR2, TAC3, or TACR3 underlie isolated hypogonadotropic hypogonadism (IHH) with clinically variable phenotypes, and, by causing incomplete intrauterine activation of the hypothalamic-pituitary-gonadal axis, may lead to cryptorchidism. To investigate the role of defects in these gene...

journal_title：Molecular and cellular endocrinology

authors： Laitinen EM,Tommiska J,Virtanen HE,Oehlandt H,Koivu R,Vaaralahti K,Toppari J,Raivio T

更新日期：2011-07-20 00:00:00

abstract：:Diabetes exacerbates neuronal injury mediated through neurotransmitters deregulation in cerebral cortex. Our study analyzed the neuroprotective effect of curcumin to prevent cortical dysfunction associated with diabetes. Our study revealed decreased gene expression of muscarinic M1, insulin receptor, SOD, choline acet...

journal_title：Molecular and cellular endocrinology

authors： Peeyush Kumar T,Antony S,Soman S,Kuruvilla KP,George N,Paulose CS

更新日期：2011-01-01 00:00:00

abstract：:Adenomas and nodules of the human adrenal cortex are common, whereas adrenocortical carcinomas are rare. Genes such as IGF2 have been suggested to be important in human adrenocortical tumorigenesis but their role has not been directly investigated. We describe here elements of a system in which hypotheses concerning t...

journal_title：Molecular and cellular endocrinology

authors： Cardoso CC,Bornstein SR,Hornsby PJ

更新日期：2009-03-05 00:00:00

abstract：:Glucocorticoids (GCs) exert profound effects on bone and are essential for human osteoblast differentiation. However, GCs are still interpreted as negative regulators of bone formation, mainly caused by the detrimental effects on bone after clinical use of GCs. In this paper we emphasize the importance of GCs for prop...

journal_title：Molecular and cellular endocrinology

authors： Eijken M,Koedam M,van Driel M,Buurman CJ,Pols HA,van Leeuwen JP

更新日期：2006-03-27 00:00:00

abstract：:Recent evidences point to a role of estrogens in males. We have earlier reported that tamoxifen, a synthetic non-steroidal antiestrogen, when administered to adult male rats, in the dose range of 0.04-0.4 mg/kg per day, reduced fertility. The reduced fertility was measured in terms of fertility index (a measure of the...

journal_title：Molecular and cellular endocrinology

authors： Balasinor N,Parte P,Gill-Sharma MK,Juneja HS

更新日期：2001-06-10 00:00:00