Abstract：
:The CYP11A1 gene encodes cytochrome P450scc, the enzyme catalyzing the first step of steroid biosynthesis in the adrenal and gonad. We generated transgenic mice containing 2.3 kb of the 5'-flanking region of CYP11A1 driving LacZ reporter gene expression, in order to study hormonal control of CYP11A1 gene expression in different tissues. This 2.3 kb fragment contains information for hormonal control; by ACTH and hCG which increased reporter gene expression, in the adrenal and testis of transgenic mice respectively, while dexamethasone administration decreased reporter activity in the adrenal. The 5'-fragment of CYP11A1 has appreciable promoter activities in mouse adrenal Y1 cells but not in non-steroidogenic COS-1 cells, showing cell-type specificity. Transcription factor SF-1 activates the 2.3 kb promoter, which can be potentiated by cotransfection with c-Jun in steroidogenic JEG3 cells but not in COS-1 cells. We conclude that the 2.3 kb region of CYP11A1 contains elements controlling hormonal-dependent, cell-type-specific expression. In addition, c-Jun and SF-1 could act synergistically to activate CYP11A1 gene expression.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Huang Y,Hu M,Hsu N,Wang CL,Chung Bdoi
10.1016/s0303-7207(01)00388-4keywords:
subject
Has Abstractpub_date
2001-04-25 00:00:00pages
205-10issue
1-2eissn
0303-7207issn
1872-8057pii
S0303720701003884journal_volume
175pub_type
杂志文章abstract：:Many agrochemicals have endocrine disrupting properties. A subset of these chemicals is characterized as "estrogenic". In this review, we describe several distinct ways that chemicals used in crop production can affect estrogen signaling. Using three agrochemicals as examples (DDT, endosulfan, and atrazine), we illust...
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
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