Human FSH isoforms: carbohydrate complexity as determinant of in-vitro bioactivity.

abstract：:Transcription of the glycoprotein hormone alpha-subunit gene in the pituitary is governed by different promoter elements in thyrotropes and gonadotropes. We recently identified an upstream enhancer that directs a high level of cell type specific expression in transgenic mice and stimulates proximal promoter activity i...

journal_title：Molecular and cellular endocrinology

authors： Wood WM,Dowding JM,Sarapura VD,McDermott MT,Gordon DF,Ridgway EC

更新日期：1998-07-25 00:00:00

abstract：:There are multiple systems for cellular cholesterol delivery for steroidogenesis, including uptake of lipoprotein-derived cholesterol via LDL receptor mediated endocytic pathways and SR-BI mediated "selective" pathways, as well as from endogenous cholesterol synthesis and the mobilization of stored cholesteryl esters....

journal_title：Molecular and cellular endocrinology

authors： Kraemer FB

更新日期：2007-02-01 00:00:00

abstract：:In the frog Xenopus laevis, vitellogenin is the major estrogen-induced protein in the liver. We have characterized an additional secreted protein, of 45,000 Da and designated Ep45, which is also responsive to estrogen treatment. Like vitellogenin, Ep45 is not normally found in the plasma nor synthesized by the liver o...

journal_title：Molecular and cellular endocrinology

authors： Holland LJ,Wangh LJ

更新日期：1987-01-01 00:00:00

abstract：:The primary aim of this study was to evaluate the effects of arachidonic acid (AA) on calcium mobilization from intracellular compartments in digitonin-permeabilized granulosa cells isolated from the largest preovulatory follicles of laying hens. At low concentrations (ED50 0.2 microM) AA released 35% 45Ca from the en...

journal_title：Molecular and cellular endocrinology

authors： Hertelendy F,Molnár M,Jamaluddin M

更新日期：1992-02-01 00:00:00

abstract：:Clinical observations indicate that elevated aldosterone impairs cardiovascular function. The mechanisms, however, are not totally understood although total and cardiovascular mortality are decreased by aldosterone antagonists. Experimentally, increased plasma aldosterone induces pericoronary inflammation and cardiac ...

journal_title：Molecular and cellular endocrinology

authors： Heymes C,Garnier A,Fuchs S,Bendall JK,Nehme J,Ambroisine ML,Robidel E,Swynghedauw B,Milliez P,Delcayre C

更新日期：2004-03-31 00:00:00

abstract：:At 14 days of age, hypothyroid and normal rats are rendered hypoglycaemic by insulin injection and the subsequent induction in adrenal tyrosine hydroxylase (TH) is studied. Hypothyroidism impairs both the intensity and the time-course of adrenal TH induction. ...

journal_title：Molecular and cellular endocrinology

authors： Gripois D,Valens M

更新日期：1984-11-01 00:00:00

abstract：:The inhibins are secreted alpha:beta heterodimers of the TGF-beta superfamily that are mainly synthesized in Sertoli cells and granulosa cells, and are critical regulators of testicular and ovarian development and function. Mice homozygous for a targeted deletion of the inhibin alpha subunit gene (Inha(-/-)) develop s...

journal_title：Molecular and cellular endocrinology

authors： Nagaraja AK,Agno JE,Kumar TR,Matzuk MM

更新日期：2008-11-06 00:00:00

abstract：:A comparative study of hGH and IL2 post-signaling effects, as examined by RNA expression (Nb29) and protein levels of the heat-shock protein hsp70, was performed in a hormone-dependent rat lymphoma cell line, Nb2-11C. Optimal doses of hGH or IL2 increased Nb29 expression in a dose-dependent manner. Addition of both mi...

journal_title：Molecular and cellular endocrinology

authors： Horn S,Cohen R,Gertler A

更新日期：1994-11-01 00:00:00

abstract：:As deduced on the basis of cloning experiments, the putative extracellular domain of pituitary glycoprotein hormone (lutropin (LH), thyrotropin (TSH), and FSH) receptors (rec) is sufficiently large to suggest involvement in hormone binding. Comparison of the amino acid sequences of the extracellular domains of the gly...

journal_title：Molecular and cellular endocrinology

authors： Dattatreyamurty B,Reichert LE Jr

更新日期：1992-09-01 00:00:00

abstract：:The conversion of 3 beta-hydroxy-5-ene steroids by the enzyme complex 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) is an obligatory step in the biosynthesis of all classes of hormonal steroids in classical steroidogenic as well as in peripheral tissues. To develop a model more closely rel...

journal_title：Molecular and cellular endocrinology

authors： Simard J,Melner MH,Breton N,Low KG,Zhao HF,Periman LM,Labrie F

更新日期：1991-02-01 00:00:00

abstract：:Employing RT- and RACE-PCR on RNA isolated from testicular tissue, we have cloned the coding cDNA sequence for the RLF, also known as Insl3, of the fallow deer. The RLF coding sequence consisted of 396 bp encoding a peptide of 131 amino acids and shared highest homology with bovine, sheep and goat RLF. Northern analys...

journal_title：Molecular and cellular endocrinology

authors： Hombach-Klonisch S,Kauffold J,Rautenberg T,Steger K,Tetens F,Fischer B,Klonisch T

更新日期：2000-01-25 00:00:00

abstract：:Diabetic cardiomyopathy is characterized by an abnormal oxidative metabolism, but the underlying mechanisms remain to be defined. To uncover potential mechanisms involved in the pathophysiology of diabetic cardiomyopathy, we performed a gene expression profiling study in hearts of diabetic db/db mice. Diabetic hearts ...

journal_title：Molecular and cellular endocrinology

authors： Lasheras J,Vilà M,Zamora M,Riu E,Pardo R,Poncelas M,Cases I,Ruiz-Meana M,Hernández C,Feliu JE,Simó R,García-Dorado D,Villena JA

更新日期：2016-07-15 00:00:00

abstract：:After i.m. injection of [3H]butyrobetaine into rats, the accumulation of carnitine into the epididymis, prostate gland, seminal vesicles, testis and heart was studied. The concentration of radiolabeled carnitine into the cauda epididymis increased linearly with time up to 72 h after the injection of the precursor, whi...

journal_title：Molecular and cellular endocrinology

authors： Bohmer T,Hansson V

更新日期：1975-08-01 00:00:00

abstract：:The transcriptional activity of various lengths of the 5'-untranslated region (UTR) of the murine LH receptor (R) gene were studied using the luciferase reporter gene in transiently transfected mouse Leydig tumor cells (mLTC-1). Chinese hamster ovarian (CHO) and HeLa cells were used as controls. The basal transcriptio...

journal_title：Molecular and cellular endocrinology

authors： El-Hefnawy T,Krawczyk Z,Nikula H,Viherä I,Huhtaniemi I

更新日期：1996-05-31 00:00:00

abstract：:Androgen receptor mRNA was translated in vitro, and androgen- and antiandrogen-bound receptor complexes were studied using limited proteolytic digestion by trypsin. Partial proteolysis of androgen-bound receptor protein resulted in a 29-kDa proteolysis-resisting fragment, whereas antiandrogen binding stabilised a 35-k...

journal_title：Molecular and cellular endocrinology

authors： Kuil CW,Mulder E

更新日期：1994-06-01 00:00:00

abstract：:We have previously shown that osteocalcin synthesis is readily induced by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in MG-63 human osteosarcoma cells (Mahonen et al. (1990) Biochim. Biophys. Acta 1048, 30-37). In the present study, the regulation of osteocalcin synthesis by other hormones of the steroid-thyroid hormone f...

journal_title：Molecular and cellular endocrinology

authors： Pirskanen A,Mahonen A,Mäenpää PH

更新日期：1991-04-01 00:00:00

abstract：:The influence of drug-receptor binding kinetics has often been overlooked during the development of new therapeutics that target G protein-coupled receptors (GPCRs). Over the last decade there has been a growing understanding that an in-depth knowledge of binding kinetics at GPCRs is required to successfully target th...

journal_title：Molecular and cellular endocrinology

authors： Sykes DA,Stoddart LA,Kilpatrick LE,Hill SJ

更新日期：2019-04-05 00:00:00

abstract：:The fibromuscular stroma (FMS) separated from the seminal vesicles of adult guinea pigs incorporated [35S]methionine into a 12 500 X g soluble fraction in vitro. Incorporation decreased 60% in tissues from 7-day castrates with no further change up to 21 days post-castration. Administration of 5 alpha-dihydrotestostero...

journal_title：Molecular and cellular endocrinology

authors： Weinberger MJ

更新日期：1984-12-01 00:00:00

abstract：:Hyperthyroidism was induced in rats and somatic indices and metabolic parameters were analyzed in testis. In addition, the morphological analysis evidenced testes maturation and intense protein synthesis and processing, supporting the enhancement in vimentin synthesis in hyperthyroid testis. Furthermore, vimentin phos...

journal_title：Molecular and cellular endocrinology

authors： Zamoner A,Barreto KP,Filho DW,Sell F,Woehl VM,Guma FC,Silva FR,Pessoa-Pureur R

更新日期：2007-03-15 00:00:00

abstract：:We have studied a 15-year-old girl (P1) suffering from the Hutchinson-Gilford syndrome (progeria) associated with a severe insulin resistance. Insulin binding activity to P1 erythrocytes was 85% reduced when compared to that measured in ten normal controls matched for sex and age. This finding was confirmed in Epstein...

journal_title：Molecular and cellular endocrinology

authors： Briata P,Bellini C,Vignolo M,Gherzi R

更新日期：1991-01-01 00:00:00

abstract：:Several forms of phosphodiesterase are present in the male gonad, and their relative concentrations vary during testicular development. On the basis of kinetic analysis and chromatography on DEAE cellulose, 3 forms were separated: (a) a high-affinity cGMP phosphodiesterase, regulated by Ca2+ and calmodulin, similar to...

journal_title：Molecular and cellular endocrinology

authors： Geremia R,Rossi P,Pezzotti R,Conti M

更新日期：1982-09-01 00:00:00

abstract：:The present work describes the identification of (anti)progestin endocrine disrupting chemicals (EDC) using a two step screening system. In the first step a competitive binding assay was developed using recombinant human progesterone receptor (hPR). The tested chemicals were of various classes like insecticides, their...

journal_title：Molecular and cellular endocrinology

authors： Viswanath G,Halder S,Divya G,Majumder CB,Roy P

更新日期：2008-11-25 00:00:00

abstract：:Catecholamines are known to have an inhibitory effect on oviductal smooth musculature by changing the adrenergic receptors activity. In order to further investigate the role of epinephrine and/or norepinephrine in oviduct, the distribution of beta-adrenergic receptors has been studied in the rat oviduct, using in vitr...

journal_title：Molecular and cellular endocrinology

authors： Tolszczuk M,Pelletier G

更新日期：1988-11-01 00:00:00

abstract：:Estrogens are synthesized from C19 steroids by a unique form of cytochrome P450, aromatase cytochrome P-450 (P-450AROM; the product of the CYP19 gene). We have shown that tissue-specific expression of human P-450AROM is determined, in part, by the use of alternative promoters. Previous methods of analysis for determin...

journal_title：Molecular and cellular endocrinology

authors： Jenkins C,Michael D,Mahendroo M,Simpson E

更新日期：1993-11-01 00:00:00

abstract：:The availability of regulatory sequences directing tissue-specific expression of transgenes in genetically modified mice and large animals is a prerequisite for the development of adequate models for human diseases. The rat insulin 2 gene (Ins2) promoter, widely used to achieve transgene expression in pancreatic beta-...

journal_title：Molecular and cellular endocrinology

authors： Grzech M,Dahlhoff M,Herbach N,Habermann FA,Renner-Müller I,Wanke R,Flaswinkel H,Wolf E,Schneider MR

更新日期：2010-02-05 00:00:00

abstract：:The growing burden of obesity- and aging-related diseases has hastened the search for governing biological processes. Cellular senescence is a stress-induced state of stable growth arrest strongly associated with aging that is aberrantly activated by obesity. The transition of a cell to a senescent state is demarcated...

journal_title：Molecular and cellular endocrinology

authors： Schafer MJ,Miller JD,LeBrasseur NK

更新日期：2017-11-05 00:00:00

abstract：:An accurate understanding of the molecular events governing pancreas development can have an impact on clinical medicine related to diabetes, obesity and pancreatic cancer, diseases with a high impact in public health. Until 1996, the main animal models in which pancreas formation and differentiation could be studied ...

journal_title：Molecular and cellular endocrinology

authors： Tiso N,Moro E,Argenton F

更新日期：2009-11-27 00:00:00

abstract：:The introduction of a targeted insertion mutation into exon 2 of the gene coding for the glucocorticoid receptor (GR) enabled production of glucocorticoid receptor knock-out (GRKO) mice. GRKO mice on a C57BL/6/129sv mixed genetic background show a variable phenotype, with 90% of -/- mice dying at birth with respirator...

journal_title：Molecular and cellular endocrinology

authors： Cole TJ,Myles K,Purton JF,Brereton PS,Solomon NM,Godfrey DI,Funder JW

更新日期：2001-02-28 00:00:00

abstract：:In this study we examined the mode of triiodothyronine (T3) and tetraiodothyronine (T4) transport in synaptosomal preparations from cerebral hemispheres of adult rat brain. Our results show that these hormones are transported by different mechanisms: T3 uptake is a saturable process and Hofstee analysis of the data re...

journal_title：Molecular and cellular endocrinology

authors： Kastellakis A,Valcana T

更新日期：1989-12-01 00:00:00

abstract：:Various water-insoluble cannabinoids as well as SP-111A, the water-soluble derivative of delta 9-tetrahydrocannabinol (delta 9-THC), reduced hCG and dibutyryl-cAMP stimulated testosterone production by rat testicular Leydig cell preparations. With 0.15 microM (0.05 micrograms/ml) 8-beta-OH-delta 9-THC the inhibition w...

journal_title：Molecular and cellular endocrinology

authors： Jakubovic A,McGeer EG,McGeer PL

更新日期：1979-07-01 00:00:00