Abstract:
:The distribution and molecular forms of bombesin-like immunoreactivity (BLI) were determined in the ovine median eminence using a new, C-terminally directed antiserum. BLI was confined to neurons of the external zone of the median eminence, near small blood vessels, many of which also were immunoreactive for CRF. Each median eminence contained about 10 pmol of BLI (533 pmol/g tissue). Gel filtration and reverse-phase high pressure liquid chromatography (HPLC) demonstrated the existence of two molecular forms of BLI, which co-eluted with porcine gastrin releasing peptide (GRP)1-27 and GRP18-27 in a molar ratio of 1:2. The presence of BLI in the ovine median eminence and its co-localization in some neurons with CRF, suggest a possible role for peptides of the bombesin family in the regulation of pituitary function.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Giraud AS,Smith AI,Rundle SEdoi
10.1016/0303-7207(87)90180-8subject
Has Abstractpub_date
1987-10-01 00:00:00pages
245-50issue
3eissn
0303-7207issn
1872-8057journal_volume
53pub_type
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