Impairment of ATP-induced Ca2+ -signalling in human thyroid cancer cells.

abstract：:The availability of regulatory sequences directing tissue-specific expression of transgenes in genetically modified mice and large animals is a prerequisite for the development of adequate models for human diseases. The rat insulin 2 gene (Ins2) promoter, widely used to achieve transgene expression in pancreatic beta-...

journal_title：Molecular and cellular endocrinology

authors： Grzech M,Dahlhoff M,Herbach N,Habermann FA,Renner-Müller I,Wanke R,Flaswinkel H,Wolf E,Schneider MR

更新日期：2010-02-05 00:00:00

abstract：:The prediction of invasion or malignant behavior in PAs remains challenging. FSCN1, an actin-bundling protein, is associated with increased risk of mortality and metastasis in various cancer types. The objective of the study was to evaluate the expression of FSCN1 in 312 PAs cases, and to analyze its association with ...

journal_title：Molecular and cellular endocrinology

authors： Liu C,Gao H,Cao L,Gui S,Liu Q,Li C,Li D,Gong L,Zhang Y

更新日期：2016-01-05 00:00:00

abstract：:Two cAMP-dependent protein kinase activities can be separated from rat thyroid cytosol. Their elution properties during the gel chromatography, as well as their sedimentation coefficients after sucrose-gradient ultracentrifugation and their capacity to dissociate in the presence of histones, suggest they are like the ...

journal_title：Molecular and cellular endocrinology

authors： Delbauffe D,Ohayon R,Pavlovic-Hournac M

更新日期：1979-05-01 00:00:00

abstract：:Placental leptin secretion has important implications for maternal adaptation to pregnancy, fetal growth and development, and local autocrine/paracrine actions within trophoblast. In this study we used a cell culture insert model to examine directional secretion of leptin from the basal and apical surfaces of human ch...

journal_title：Molecular and cellular endocrinology

authors： Wyrwoll CS,Mark PJ,Waddell BJ

更新日期：2005-09-28 00:00:00

abstract：:The molecular cloning and nucleotide sequencing of the gonadotropin-releasing hormone (GnRH) receptor represented an enhanced step in the experimental effort to understand this key molecule in the reproductive process at a cell and molecular level. A subsequent step in this broad effort is heterologous expression of t...

journal_title：Molecular and cellular endocrinology

authors： Neill JD,Sellers JC,Musgrove LC,Duck LW

更新日期：1997-03-28 00:00:00

abstract：:alpha-Fetoprotein (AFP) is a serum protein expressed during fetal life, the expression of which is shut off after birth. The activity of the mouse Afp gene promoter region comprised between -80 and -38 bp is regulated by the thyroid hormone receptor (T3R): negatively in the presence of T3 and positively in the absence...

journal_title：Molecular and cellular endocrinology

authors： Van Reeth T,Gabant P,Szpirer C,Szpirer J

更新日期：2002-02-25 00:00:00

abstract：:We studied the functional properties of hepatic glucagon receptors during rat development. Glucagon binding to liver membranes and isolated hepatocytes was significantly less in foetuses and weaning rats than in adult animals, reflecting changes in the number of receptors rather than any change in receptor affinity fo...

journal_title：Molecular and cellular endocrinology

authors： Blázquez E,Perez Castillo A,de Diego JG

更新日期：1987-02-01 00:00:00

abstract：:The present study examined the influence of the calcium ionophores A23187 and X537A on the calcitonin (CT) secretory process. The isolated perfused porcine thyroid was used to evaluate ionophore effects on CT secretion and thyroid slices were used to measure 45Ca uptake. Both A23187 and X537A enhanced the rate of CT r...

journal_title：Molecular and cellular endocrinology

authors： Pento JT

更新日期：1986-04-01 00:00:00

abstract：:The effects of a GnRH antagonist analogue (N-acetyl-Ala1,D-p-Cl-Phe2,D-Trp3,6-GnRH, Ant.) and a GnRH antiserum (A/S) on the development of pituitary-testicular function were studied in immature (23/24-31/32-day-old) rats. In another experiment the Ant. treatment was combined with bromocriptine (BR)-induced hypoprolact...

journal_title：Molecular and cellular endocrinology

authors： Huhtaniemi IT,Stewart JM,Channabasavaiah K,Fraser HM,Clayton RN

更新日期：1984-02-01 00:00:00

abstract：:The present study investigated the pattern and site of expression of the prolactin receptor gene in the testis of the seasonally breeding Soay sheep. In experiment 1, Northern blot analysis confirmed expression of the prolactin receptor gene in the testis which was encoded by RNA transcripts of approximately 3.6, 11.2...

journal_title：Molecular and cellular endocrinology

authors： Jabbour HN,Lincoln GA

更新日期：1999-02-25 00:00:00

abstract：:Melatonin has repeatedly been shown to inhibit the proliferation of MCF-7 human breast cancer cells. Previous reports suggest that the actions of melatonin can be mediated either through G-protein coupled membrane receptors or via retinoid orphan receptors (RORalphas). In this study, we demonstrated the expression of ...

journal_title：Molecular and cellular endocrinology

authors： Dai J,Ram PT,Yuan L,Spriggs LL,Hill SM

更新日期：2001-05-15 00:00:00

abstract：:We have shown previously that activin A increases the number of immunoreactive follicle-stimulating hormone (FSH) cells. To further investigate the action of activin A, we examined its effects on anterior pituitary cells fractionated by centrifugal elutriation. Before activin A treatment, FSH cells were widely distrib...

journal_title：Molecular and cellular endocrinology

authors： Katayama T,Shiota K,Takahashi M

更新日期：1991-05-01 00:00:00

abstract：:Skeletal muscle is the major site of postprandial glucose disposal and augmenting glucose uptake into this tissue may attenuate insulin resistance that precedes type 2 diabetes mellitus. Here, we investigated the effect of pongamol, an identified lead molecule from the fruits of Pongamia pinnata, on glucose uptake and...

journal_title：Molecular and cellular endocrinology

authors： Tamrakar AK,Jaiswal N,Yadav PP,Maurya R,Srivastava AK

更新日期：2011-06-06 00:00:00

abstract：:The presence of insulin resistance is increasingly recognized as an important contributor to early stage kidney disease independent of the contribution of diabetes. Important in this relationship is the strong correlation between hyperinsulinemia and low levels of albuminuria (e.g. microalbuminuria). Recent work highl...

journal_title：Molecular and cellular endocrinology

authors： Nistala R,Whaley-Connell A

更新日期：2013-09-25 00:00:00

abstract：:Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are essential factors in mammary-gland (MG) development and are altered during fasting. However, no studies have investigated the alterations in the expression of GH/IGF-I and its regulatory systems (somatostatin/cortistatin and ghrelin) in MG during fasting...

journal_title：Molecular and cellular endocrinology

authors： Villa-Osaba A,Gahete MD,Cordoba-Chacon J,de Lecea L,Castaño JP,Luque RM

更新日期：2016-10-15 00:00:00

abstract：OBJECTIVES:The aim of this study is to address the role of low-density lipoprotein (LDL) on mouse preimplantation embryos. METHODS:The temporal expression of low-density lipoprotein receptor (LDLR) and side-chain cleavage cytochrome P450 (P450scc) mRNAs in mouse oocytes and preimplantation embryos up to the hatched bl...

journal_title：Molecular and cellular endocrinology

authors： Sato N,Kawamura K,Fukuda J,Honda Y,Sato T,Tanikawa H,Kodama H,Tanaka T

更新日期：2003-04-28 00:00:00

abstract：:Protection of the spermatogenic cells from the host immune response is fundamental to male fertility. Significantly, this protection extends to the tolerance of foreign tissue grafts placed within the testicular environment, a phenomenon that is called 'immune privilege'. This privilege of the testis appears to involv...

journal_title：Molecular and cellular endocrinology

authors： Meinhardt A,Hedger MP

更新日期：2011-03-15 00:00:00

abstract：:The androgen receptor (AR) is a ligand-dependent transcription activator responsible for male sexual development. In order to specifically inhibit the AR pathway, dominant negative ARs were constructed by inactivation of the major transactivation domains of the wild type AR and fusing this mutant (AR122) to the Krüppe...

journal_title：Molecular and cellular endocrinology

authors： Bramlett KS,Dits NF,Sui X,Jorge MC,Zhu X,Jenster G

更新日期：2001-10-25 00:00:00

abstract：:Administration of hydrocortisone to adrenalectomised rats elevated levels of both free and membrane-bound polysomes in liver and led to marked shifts in their sedimentation profiles in favour of heavier polysomes. The rate of nascent polypeptide formation was also found to be enhanced in response to the hormone but th...

journal_title：Molecular and cellular endocrinology

authors： Kulkarni SB,Netrawali MS,Pradhan DS,Sreenivasan A

更新日期：1976-02-01 00:00:00

abstract：:The peculiarities of cellular and tissue distribution of prolactin receptors (PRLR) in the liver of female and male rats with different sex steroid status were investigated in paraplast-embedded tissue with the indirect immunoperoxidase technique. Two clones of antibodies directed outside the PRL-binding site (U6) or ...

journal_title：Molecular and cellular endocrinology

authors： Smirnova OV,Petraschuk OM,Kelly PA

更新日期：1994-10-01 00:00:00

abstract：:The Klotho gene encodes a single-pass transmembrane protein and functions as an aging-suppressor gene, which extends lifespan when overexpressed and accelerates the development of aging-like phenotypes when disrupted in mice. Fibroblast growth factor 23 (FGF23) is a bone-derived hormone that regulates phosphate and vi...

journal_title：Molecular and cellular endocrinology

authors： Kurosu H,Kuro-O M

更新日期：2009-02-05 00:00:00

abstract：:Thrombin and COX-2 regulating cardiac hypertrophy are via various signaling cascades. Several transcriptional factors including CREB involve in COX-2 expression. However, the interplay among thrombin, CREB, and COX-2 in primary human neonatal ventricular cardiomyocytes remains unclear. In this study, thrombin-induced ...

journal_title：Molecular and cellular endocrinology

authors： Chien PT,Lin CC,Hsiao LD,Yang CM

更新日期：2015-07-05 00:00:00

abstract：:The intranuclear locations at which rat uterine estrogen receptors interact with chromatin have been probed using digestions performed with DNAase I and micrococcal nuclease. Exposure to nuclease has been controlled to effect limited to extensive digestion of nuclear DNA under conditions which maintain the integrity o...

journal_title：Molecular and cellular endocrinology

authors： Pavlik EJ,Katzenellenbogen BS

更新日期：1982-04-01 00:00:00

abstract：:We examined the effects of growth hormone (GH), prolactin (PRL) and insulin-like growth factor-I (IGF-I) on DNA synthesis, and insulin-like growth factor binding protein 3 (IGFBP-3) gene expression, in a rat beta-cell line INS-1. GH and PRL were equally effective in increasing DNA synthesis and IGFBP-3 gene expression...

journal_title：Molecular and cellular endocrinology

authors： De W,Bréant B,Czernichow P,Asfari M

更新日期：1995-10-30 00:00:00

abstract：:The immune system and the neuroendocrine system are functionally interactive. Lymphocytes possess opioid receptors as well as growth hormone receptors (GHR) by which opioids and growth hormone (GH) may modulate immune functions. In this study, we have investigated the effects of morphine on [125I]hGH binding and GHR g...

journal_title：Molecular and cellular endocrinology

authors： Henrohn D,Le Grevés P,Nyberg F

更新日期：1997-12-12 00:00:00

abstract：:Stanniocalcin-1 (STC-1) is made by kidney collecting duct cells for autocrine and paracrine targeting of nephron cell mitochondria. Here, the ligand stimulates respiratory uncoupling and calcium uniport activity. However, the underlying purpose of these actions and how the renal gene is regulated are poorly understood...

journal_title：Molecular and cellular endocrinology

authors： Turner J,Xiang FL,Feng Q,Wagner GF

更新日期：2011-01-01 00:00:00

abstract：:The thyroid hormone receptors (TR) are able to bind DNA and to repress transcription in the absence of thyroid hormone. This repression function is an important feature of TRs as aberrant silencing can lead to severe diseases and developmental abnormalities. TR utilizes different mechanisms to achieve repression of ta...

journal_title：Molecular and cellular endocrinology

authors： Eckey M,Moehren U,Baniahmad A

更新日期：2003-12-31 00:00:00

abstract：:Autodigestion of nuclei isolated from the androgen-dependent Shionogi mouse mammary carcinoma revealed the presence of endogenous endonuclease activity which was stimulated by Ca2+ and Mg2+ and generated a repeating pattern of DNA fragments with an average monomeric size of 179 base pairs. The nuclear concentration of...

journal_title：Molecular and cellular endocrinology

authors： Giles MG,Rennie PS,Bruchovsky N

更新日期：1986-05-01 00:00:00

abstract：:The chum salmon insulin-like growth factor II (IGF-II) gene is highly expressed in liver tissue. In this study we demonstrate that two transcription factors, Sp1 and C/EBPbeta, are involved in the enhanced expression of the salmon IGF-II gene. The presence of the fish homolog for C/EBPbeta in salmon liver RNA was conf...

journal_title：Molecular and cellular endocrinology

authors： Palamarchuk AY,Kavsan VM,Sussenbach JS,Holthuizen PE

更新日期：2001-02-14 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) (1,25D(3)), the active metabolite of vitamin D(3), inhibits breast cancer cell growth in vivo and in vitro. To examine mechanisms of 1,25D(3) induced growth arrest and apoptosis, cell lines were established from DMBA induced mammary tumors derived from vitamin D(3) receptor knockout (VDRKO) ...

journal_title：Molecular and cellular endocrinology

authors： Zinser GM,McEleney K,Welsh J

更新日期：2003-02-28 00:00:00