Abstract:
:The peculiarities of cellular and tissue distribution of prolactin receptors (PRLR) in the liver of female and male rats with different sex steroid status were investigated in paraplast-embedded tissue with the indirect immunoperoxidase technique. Two clones of antibodies directed outside the PRL-binding site (U6) or to the PRL-binding site (T6) of the receptor were used. PRLR-specific immunoreactivity was identified essentially in hepatocytes. PRLR can be visualized in sinusoidal domains of cellular membranes, in cytoplasmic granules and sometimes in the perinuclear area of hepatocytes. The staining characteristics were similar with both antibodies. There were no prominent differences in the intensity of PRLR-positive staining among hepatocytes of different zones of hepatic lobules with the exception of some hepatocytes around central veins. Sex differences in the intensity of immunostaining (strong in females, and faint in males) but not in the amount and distribution of PRLR-containing cells were observed. Gonadectomy of animals caused the disappearance of sex differences in the intensity of PRLR-positive staining as a result of its decrease in females and increase in males. The essential elevation in the intensity of PRLR-specific immunoreactivity was revealed in hepatocytes of gonadectomized females and males after prolonged estradiol administration (10 micrograms for 14 days). The cytoplasmic staining of some hepatocytes surrounding central veins was much more pronounced in estrogenized animals. PRLR-specific immunoreactivity in the perinuclear area was identified in these cells. Androgen treatment (3 mg of testosterone-propionate for 3 days) of gonadectomized animals caused a decrease in the intensity of hepatocyte PRLR-positive staining similarly in both sexes.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Smirnova OV,Petraschuk OM,Kelly PAdoi
10.1016/0303-7207(94)90037-xsubject
Has Abstractpub_date
1994-10-01 00:00:00pages
77-81issue
1eissn
0303-7207issn
1872-8057journal_volume
105pub_type
杂志文章abstract::The regulation of heat shock protein 90 kDa (hsp90) by estradiol was analyzed in the rat ventromedial hypothalamus (VMH) and uterus by one-dimensional gel electrophoresis/Western blots. Protein from VMH and uterus (35 micrograms/sample) was resolved on 8% acrylamide gels, transferred to polyvinyldifluoride filters, an...
journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(92)90028-5
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abstract::It has been well established for decades that androgens, namely testosterone (T) plays an important role in female reproductive physiology as the precursor for oestradiol (E2). However, in the last decade a direct role for androgens, acting via the androgen receptor (AR), in female reproductive function has been confi...
journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
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journal_title:Molecular and cellular endocrinology
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doi:10.1016/0303-7207(90)90070-o
更新日期:1990-05-28 00:00:00
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2010-10-07 00:00:00
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(96)03774-4
更新日期:1996-04-19 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/s0303-7207(02)00036-9
更新日期:2002-06-28 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(96)03826-9
更新日期:1996-07-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2018.05.015
更新日期:2018-12-05 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2010.02.009
更新日期:2010-05-14 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/s0303-7207(00)00288-4
更新日期:2000-09-25 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/s0303-7207(99)00178-1
更新日期:1999-12-20 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2002-02-25 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2005.09.010
更新日期:2005-11-24 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2011.07.036
更新日期:2012-01-02 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(95)03640-s
更新日期:1995-10-30 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.mce.2011.04.013
更新日期:2011-06-20 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(92)90251-z
更新日期:1992-09-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
doi:10.1016/s0303-7207(01)00646-3
更新日期:2001-11-26 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
doi:10.1016/0303-7207(95)03575-r
更新日期:1995-06-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2015.12.013
更新日期:2016-02-15 00:00:00