当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Na+/H+ exchange is responsible for intracellular pH regulation in insulin-secreting HIT-T15 cells.

Na+/H+ exchange is responsible for intracellular pH regulation in insulin-secreting HIT-T15 cells.

Abstract：

:The addition of glucose to suspensions of HIT-T15 insulinoma cells caused a small, transient acidification followed by a gradual, progressive alkalinisation, as assessed by the fluorescent pH-sensitive dye 2',7'-biscarboxyethyl-5'-(6')-carboxyfluorescein (BCECF). Treatment of cells with acetate or lactate produced an immediate, marked acidification followed by recovery and a subsequent alkalinisation. In contrast, addition of NH4Cl caused a rapid rise in intracellular pH (pHi) and recovery to resting values. In cells where Na+/H+ exchange was inhibited, either with amiloride or by omission of Na+ from the medium, glucose caused a progressive acidification, whilst recovery from acetate- or lactate-induced acidification was prevented. Under sodium-free conditions, recovery from acidification could be initiated by addition of Na+. Inhibition of HCO3-/Cl- exchange by pretreatment with 4,4'-diisothiocyanatostilbene 2,2'-disulphonic acid (DIDS), or by omission of HCO3- or Cl- from the medium did not affect any of the changes in pHi elicited by the above agents. It is concluded that the principal mechanism responsible for pHi regulation in HIT-T15 cells is the Na+/H+ antiporter and that the HCO3-/Cl- exchange systems make little, if any, contribution.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Trebilcock R,Lynch A,Tomlinson S,Best L

doi

10.1016/0303-7207(90)90070-o

subject

Has Abstract

pub_date

1990-05-28 00:00:00

pages

21-5

issue

1

eissn

0303-7207

issn

1872-8057

pii

0303-7207(90)90070-O

journal_volume

71

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Assessment of the role of cyclic nucleotides in allatostatin-induced inhibition of juvenile hormone biosynthesis in Diploptera punctata.

    abstract：:In an effort to identify the signal transduction mechanism associated with the inhibition of juvenile hormone (JH) biosynthesis by the neuropeptides allatostatins, levels of the cyclic nucleotides cAMP and cGMP were measured in corpora allata (CA) of virgin and mated Diploptera punctata females using radioimmunoassays...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(92)90218-u

    authors： Cusson M,Yagi KJ,Guan XC,Tobe SS

    更新日期：1992-11-01 00:00:00

  • Ontogenesis of the in vitro response of rat testis to gonadotropin-releasing hormone.

    abstract：:The age-related evolution of the in vitro effects of a gonadotropin releasing hormone agonist ([D-Trp6]-GnRH) on the secretion of testosterone by the testis, cultured during 3 days on a Millipore filter floating on M199 medium, was studied during the perinatal period in the rat. The basal and luteinizing hormone (LH)-...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(91)90032-n

    authors： Habert R,Devif I,Gangnerau MN,Lecerf L

    更新日期：1991-12-01 00:00:00

  • Role of ligand-dependent GR phosphorylation and half-life in determination of ligand-specific transcriptional activity.

    abstract：:A central question in glucocorticoid mechanism of action via the glucocorticoid receptor (GR) is what determines ligand-selective transcriptional responses. Using a panel of 12 GR ligands, we show that the extent of GR phosphorylation at S226 and S211, GR half-life and transcriptional response, occur in a ligand-selec...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2010.06.007

    authors： Avenant C,Ronacher K,Stubsrud E,Louw A,Hapgood JP

    更新日期：2010-10-07 00:00:00

  • A regulation of the level of phosphorylated phospholamban by inhibitor-1 in rat heart preparations in vitro.

    abstract：:Inhibitor-1 following phosphorylation by protein kinase A inhibits phosphoprotein phosphatase-1. We have found that in the rat heart inhibitor-1 is present only in the cytosolic fraction and that its phosphorylation in ventricular slices was increased by isoproterenol but not by isoproterenol and propranolol together....

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(88)90084-6

    authors： Iyer RB,Koritz SB,Kirchberger MA

    更新日期：1988-01-01 00:00:00

  • Differential alternative splicing of PACAP receptor in pituitary cell subpopulations.

    abstract：:The capability of rat pituitary cells to express receptors for pituitary adenylate cyclase activating polypeptide (PACAP) and VIP was evaluated by binding studies and measurement of adenylate cyclase activity on whole gland preparations and by reverse transcriptase-polymerase chain reaction (TR-PCR) using specific pri...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)03626-i

    authors： Vertongen P,Velkeniers B,Hooghe-Peters E,Robberecht P

    更新日期：1995-09-22 00:00:00

  • Asprosin impairs insulin secretion in response to glucose and viability through TLR4/JNK-mediated inflammation.

    abstract：:Severe inflammation in the islets is observed in obese patients with type 2 diabetes. Inflammation in the islets is caused by obesity-induced serum free fatty acids. Asprosin is a fasting-induced adipokine, which contributes to hepatic glucose production. However, the effects of asprosin on inflammation and cellular d...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.03.001

    authors： Lee T,Yun S,Jeong JH,Jung TW

    更新日期：2019-04-15 00:00:00

  • Quantitative reverse transcription and polymerase chain reaction analysis of oxytocin and vasopressin receptor mRNAs in the rat uterus near parturition.

    abstract：:Oxytocin receptors (OT-R) are known to be involved in the course of labor since a massive increase in OT-binding sites is observed in the uterus just before parturition. Vasopressin (AVP)-binding sites have also been observed and have been shown to mediate uterotonic responses. To determine exactly which subtypes of O...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(97)00218-9

    authors： Clerget MS,Elalouf JM,Germain G

    更新日期：1997-12-31 00:00:00

  • Annexin A6 regulates adipocyte lipid storage and adiponectin release.

    abstract：:Lipid storage and adipokine secretion are critical features of adipocytes. Annexin A6 (AnxA6) is a lipid-binding protein regulating secretory pathways and its role in adiponectin release was examined. The siRNA-mediated AnxA6 knock-down in 3T3-L1 preadipocytes impaired proliferation, and differentiation of AnxA6-deple...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.09.033

    authors： Krautbauer S,Haberl EM,Eisinger K,Pohl R,Rein-Fischboeck L,Rentero C,Alvarez-Guaita A,Enrich C,Grewal T,Buechler C,Neumeier M

    更新日期：2017-01-05 00:00:00

  • A Sertoli cell-secreted paracrine factor(s) stimulates proliferation and inhibits steroidogenesis of rat Leydig cells.

    abstract：:Previous studies have shown that disruption or damage to the seminiferous tubules by radiation, antiandrogen, vitamin A deficiency or experimental cryptorchidism causes Leydig cell hypertrophy and hyperplasia, suggesting that Sertoli cells secrete a mitogenic factor(s) that stimulates Leydig cell proliferation. To stu...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)90191-0

    authors： Wu N,Murono EP

    更新日期：1994-12-01 00:00:00

  • Lack of thyroid hormone effect on activation energy of NaK-ATPase.

    abstract：:In order to differentiate whether activation of NaK-ATPase in thyroid thermogenesis is due to increased numbers of active 'sodium pump' units or due to a change in the kinetics of the enzyme, the effect of T3 on activation energy (Ea) of NaK-ATPase was determined in rat liver, kidney and brain. Injection of T3 produce...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(77)90107-1

    authors： Rahimifar M,Ismail-Beigi

    更新日期：1977-02-01 00:00:00

  • In vitro characterization and endocrine regulation of cholesterol and phospholipid transport in the mammary gland.

    abstract：:Cell-based studies previously showed that the ATP-binding cassette transporter A1 (ABCA1) transfers cholesterol across mammary epithelial cells (MEC). Data for phospholipid transport are lacking, and it is unclear from which cellular source the transported cholesterol stems, whether this transport activates signaling ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.10.016

    authors： Ontsouka CE,Huang X,Aliyev E,Albrecht C

    更新日期：2017-01-05 00:00:00

  • Spironolactone increases integrin beta3 gene expression in kidney and heart muscle cells.

    abstract：:In clinical trials of heart failure, spironolactone, an antagonist of the mineralocorticoid receptor (MR), reduced mortality rates by unknown mechanisms. We hypothesized that spironolactone functions by upregulating expression of certain cardiovascular genes. An RNA differential display technique was used to identify ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(02)00112-0

    authors： Chun TY,Bloem L,Pratt JH

    更新日期：2002-08-30 00:00:00

  • Role of epididymal receptor HE6 in the regulation of sperm microenvironment.

    abstract：:HE6 (GPR64) is a highly conserved, tissue-specific heptahelical receptor of the human epididymis. The seven transmembrane (TM7) domains are a hallmark of G-protein-coupled receptors (GPCRs) which have a proven history of being excellent therapeutic targets. Of all currently marketed drugs, >30% are modulators of speci...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2005.12.023

    authors： Kirchhoff C,Obermann H,Behnen M,Davies B

    更新日期：2006-05-16 00:00:00

  • Discordant expression of insulin-like growth factors and their receptor messenger ribonucleic acids in endometrial carcinomas relative to normal endometrium.

    abstract：:The inappropriate expressions of insulin-like growth factors (IGF-I and II) and IGF-I receptor (IGF-IR) are implicated in the malignant growth of many cancers. To determine changes, if any, in the levels of expression of IGFs and IGF receptor genes in neoplastic endometrium, relative to normal endometrium, the mRNA le...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(99)00092-1

    authors： Roy RN,Gerulath AH,Cecutti A,Bhavnani BR

    更新日期：1999-07-20 00:00:00

  • Endocannabinoids, sperm functions and energy metabolism.

    abstract：:Cannabinoids, the main active components of marijuana, have been shown to exert different adverse effects on male reproduction both in vertebrates and invertebrates. The main effects of endocannabinoids, a particular group of endogenously produced cannabinoids, in sperm are the inhibition of motility, capacitation and...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2008.02.013

    authors： Rossato M

    更新日期：2008-04-16 00:00:00

  • Metabolism of hCG and hLH to multiple urinary forms.

    abstract：:Human chorionic gonadotropin (hCG) is synthesized primarily in the placenta while human luteinizing hormone (hLH) is produced in the pituitary. Both hormones are highly homologous in structure and both appear to be altered to analogous molecular forms as the hormones are proteolytically processed, or metabolized, from...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(96)03942-1

    authors： Birken S,Kovalevskaya G,O'Connor J

    更新日期：1996-12-20 00:00:00

  • Central nervous system-specific knockout of steroidogenic factor 1.

    abstract：:Steroidogenic factor 1 (SF-1) is a nuclear receptor that plays important roles in the hypothalamus-pituitary-steroidogenic organ axis. Global knockout studies in mice revealed the essential in vivo roles of SF-1 in the ventromedial hypothalamic (VMH) nucleus, adrenal glands, and gonads. One limitation of global SF-1 k...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2008.09.026

    authors： Kim KW,Zhao L,Parker KL

    更新日期：2009-03-05 00:00:00

  • Regulation of steroidogenesis in crayfish molting glands: involvement of protein synthesis.

    abstract：:The involvement of continuous protein synthesis in the mechanisms of crustacean steroidogenesis was investigated using crayfish molting glands (Y-organs). During intermolt, Y-organ steroidogenic activity is low. Eyestalk ablation initiates premolt which is characterized by a rapid increase in the production of ecdyste...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)03489-t

    authors： Dauphin-Villemant C,Böcking D,Sedlmeier D

    更新日期：1995-03-01 00:00:00

  • Angiotensin II induces interleukin-6 expression in astrocytes: Role of reactive oxygen species and NF-κB.

    abstract：:Previously, we showed that the bio-peptide angiotensin (Ang) II induces interleukin-6 (IL-6) in cultured astrocytes; however, the mechanism(s) involved in this effect were unknown. In the current study, we determined in brainstem and cerebellum astrocytes from the spontaneously hypertensive rat (SHR), the effect of An...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.08.013

    authors： Gowrisankar YV,Clark MA

    更新日期：2016-12-05 00:00:00

  • Empagliflozin attenuates ischemia and reperfusion injury through LKB1/AMPK signaling pathway.

    abstract：:The beneficial effects of empagliflozin (EMPA) on cardiac functions during ischemia and reperfusion were characterized. The contractile functions of isolated cardiomyocytes from adult C57BL/6J mice were determined with IonOptix SoftEdgeMyoCam system. The mitochondrial superoxide production was measured by MitoSOX fluo...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.110642

    authors： Lu Q,Liu J,Li X,Sun X,Zhang J,Ren D,Tong N,Li J

    更新日期：2020-02-05 00:00:00

  • Complex prolactin crosstalk in breast cancer: new therapeutic implications.

    abstract：:The contributions of prolactin (PRL) to breast cancer are becoming increasingly recognized. To better understand the role for PRL in this disease, its interactions with other oncogenic growth factors and hormones must be characterized. Here, we review our current understanding of PRL crosstalk with other mammary oncog...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2009.03.014

    authors： Carver KC,Arendt LM,Schuler LA

    更新日期：2009-08-13 00:00:00

  • Effect of tamoxifen on sperm fertilising ability and preimplantation embryo development.

    abstract：:Recent evidences point to a role of estrogens in males. We have earlier reported that tamoxifen, a synthetic non-steroidal antiestrogen, when administered to adult male rats, in the dose range of 0.04-0.4 mg/kg per day, reduced fertility. The reduced fertility was measured in terms of fertility index (a measure of the...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(01)00428-2

    authors： Balasinor N,Parte P,Gill-Sharma MK,Juneja HS

    更新日期：2001-06-10 00:00:00

  • o,p'-DDT and its metabolites inhibit progesterone-dependent responses in yeast and human cells.

    abstract：:Using a combination of in vitro assays we have evaluated whether DDT metabolites can interact with the progesterone receptor pathway in yeast expressing human progesterone receptor (hPR) and in T47D human breast cancer cells which express endogenous hPR. In transactivation assays using both yeast and T47D cells, o,p'-...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(96)04041-5

    authors： Klotz DM,Ladlie BL,Vonier PM,McLachlan JA,Arnold SF

    更新日期：1997-04-25 00:00:00

  • A novel glucocorticoid regulatory unit mediates the hormone responsiveness of the beta1-adrenergic receptor gene.

    abstract：:The effects of glucocorticoids on expression of the beta1-adrenergic receptor (beta1AR) gene have been varied. To study the mechanism underling hormonal regulation of the beta1AR, transient transfection of progressively deleted ovine beta1AR promoter fragments was used to identify a 43-bp region (-1274 to -1232 from t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(01)00490-7

    authors： Tseng YT,Stabila JP,Nguyen TT,McGonnigal BG,Waschek JA,Padbury JF

    更新日期：2001-07-05 00:00:00

  • Cyclic AMP-dependent and independent stimulations of ovarian steroidogenesis by brain factors in the blowfly, Phormia regina.

    abstract：:The involvement of cyclic-AMP (cAMP) as a potential second messenger in the neurohormonal control of ovarian steroidogenesis was investigated in the adult female blowfly Phormia regina. Individual measurements of ovarian cAMP concentrations and of ovarian biosynthesis of ecdysteroids, stimulated after a protein meal, ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(00)00312-9

    authors： Manière G,Vanhems E,Delbecque J

    更新日期：2000-10-25 00:00:00

  • Mitochondrial signals drive insulin secretion in the pancreatic β-cell.

    abstract：:β-Cell nutrient sensing depends on mitochondrial function. Oxidation of nutrient-derived metabolites in the mitochondria leads to plasma membrane depolarization, Ca(2+) influx and insulin granule exocytosis. Subsequent mitochondrial Ca(2+) uptake further accelerates metabolism and oxidative phosphorylation. Nutrient a...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2011.07.016

    authors： Wiederkehr A,Wollheim CB

    更新日期：2012-04-28 00:00:00

  • Mammary Stat5 abundance and activity are not altered with lactation state in cows.

    abstract：:Stat5 is a key intracellular mediator of prolactin signalling and can activate transcription of milk proteins in response to prolactin. Therefore, in animals such as mice where lactation is dependent on prolactin, Stat5 is likely to play an important role in establishing or maintaining lactation in the mammary gland. ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(97)00161-5

    authors： Wheeler TT,Kuys YM,Broadhurst MM,Molenaar AJ

    更新日期：1997-10-20 00:00:00

  • Curcumin-activated autophagy plays a negative role in its anti-osteoclastogenic effect.

    abstract：BACKGROUND/PURPOSE:It remains unclear what role curcumin plays in the autophagy of osteoclast precursors (OCPs) during osteoclastogenesis, since some researchers found that curcumin has the ability to inhibit osteoclastogenesis. While others have considered it as an autophagy activator. This study aimed to determine th...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.110637

    authors： Ke D,Wang Y,Yu Y,Wang Y,Zheng W,Fu X,Han J,Zhang G,Xu J

    更新日期：2020-01-15 00:00:00

  • New and translational perspectives of oestrogen deprivation in breast cancer.

    abstract：:Over the last 20 years, aromatase inhibitors have been developed to become a highly effective treatment strategy for treatment of hormone receptor positive breast cancer. Despite their success, poor response and resistance limit the effectiveness of these agents in up to 50% of patients. In recent years, studies using...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2010.12.034

    authors： Dunbier AK,Martin LA,Dowsett M

    更新日期：2011-07-04 00:00:00

  • SIRT1 suppresses high glucose and palmitate-induced osteoclast differentiation via deacetylating p66Shc.

    abstract：:Findings concerning the role of diabetes mellitus (DM) in osteoclast differentiation are contradictory in vivo and in vitro. Sirtuin 1 (SIRT1) can inhibit RANKL-induced osteoclastogenesis and deacetylate p66Shc suppress its phosphorylation in high glucose (HG)-stimulated human umbilical vein endothelial cells. This st...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.02.015

    authors： Qu B,Gong K,Yang H,Li Y,Jiang T,Zeng Z,Cao Z,Pan X

    更新日期：2018-10-15 00:00:00