Abstract：
:The involvement of cyclic-AMP (cAMP) as a potential second messenger in the neurohormonal control of ovarian steroidogenesis was investigated in the adult female blowfly Phormia regina. Individual measurements of ovarian cAMP concentrations and of ovarian biosynthesis of ecdysteroids, stimulated after a protein meal, demonstrated that steroidogenesis is preceded by a peak of cAMP in the ovaries. In vitro, ovarian steroidogenesis was stimulated by cell-permeable analogues of cAMP and by forskolin. Crude brain extracts were also able to elicit a rise of cAMP in the ovaries in vitro and the secretion of ecdysteroids into the medium: such extracts were more active before than after the protein meal, suggesting a rapid release of neuroendocrine material after feeding. Extracts were then prepared from the dorso-medial part of the brain, containing the neurosecretory cells of the pars intercerebralis (PI): these extracts were again found to stimulate the ovarian ecdysteroid secretion, but surprisingly, they failed to trigger a rise of cAMP in the ovaries in vitro. However, extracts from the rest of the cephalic nervous mass, deprived of PI, were also steroidogenic and they increased ovarian cAMP. Experiments with Rp-cAMPS, a cAMP antagonist, were not able to prevent the ecdysteroid stimulation by PI extracts, but did so partly for the extracts deprived of PI. This study thus indicates that at least two different cephalic factors are able to stimulate ovarian steroidogenesis in the blowfly, one elaborated by PI and acting via a cAMP-independent mechanism, and the other elaborated outside PI and using cAMP as a second messenger.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Manière G,Vanhems E,Delbecque Jdoi
10.1016/s0303-7207(00)00312-9keywords:
subject
Has Abstractpub_date
2000-10-25 00:00:00pages
31-40issue
1-2eissn
0303-7207issn
1872-8057pii
S0303720700003129journal_volume
168pub_type
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
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pub_type： 杂志文章,评审
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更新日期：1999-04-25 00:00:00
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更新日期：2002-02-25 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2008-09-24 00:00:00
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pub_type： 杂志文章
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更新日期：1988-12-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2011.07.036
更新日期：2012-01-02 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2016-03-05 00:00:00
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1984-12-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2016-01-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2007.11.008
更新日期：2008-01-30 00:00:00
