当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Quantitative reverse transcription and polymerase chain reaction analysis of oxytocin and vasopressin receptor mRNAs in the rat uterus near parturition.

Quantitative reverse transcription and polymerase chain reaction analysis of oxytocin and vasopressin receptor mRNAs in the rat uterus near parturition.

Abstract：

:Oxytocin receptors (OT-R) are known to be involved in the course of labor since a massive increase in OT-binding sites is observed in the uterus just before parturition. Vasopressin (AVP)-binding sites have also been observed and have been shown to mediate uterotonic responses. To determine exactly which subtypes of OT/AVP receptors are expressed in the rat uterus near parturition, we carried out absolute quantitations of the neurohypophysial hormone receptor (OT-R and the vasopressin receptors V1a-R, V1b-R and V2-R) mRNAs with an assay based on reverse transcription-polymerase chain reaction (RT-PCR) using in vitro transcribed mutated cRNAs as internal standards. The number of mRNA molecules/ng of total RNA was 35 +/- 6, 220 +/- 33 and 39 +/- 9 for OT-R (P < 0.01) and 16 +/- 1, 25 +/- 8 and 31 +/- 5 for V1a-R (P > 0.05) on day (D) 21, 22 and 23 of gestation (post-parturient), respectively. We did not detect V1b-R and V2-R mRNAs in the pregnant uterus. Therefore, the heterogeneity of OT and AVP receptors in the rat uterus can only be assigned to the presence of OT-R and V1a-R neurohypophysial hormone receptor subtypes, whereas V1b-R and V2-R can not be invoked. Only OT-R mRNA levels change in the uterus near parturition.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Clerget MS,Elalouf JM,Germain G

doi

10.1016/s0303-7207(97)00218-9

subject

Has Abstract

pub_date

1997-12-31 00:00:00

pages

79-89

issue

1

eissn

0303-7207

issn

1872-8057

pii

S0303720797002189

journal_volume

136

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Maternal undernutrition throughout pregnancy increases adrenocorticotrophin receptor and steroidogenic acute regulatory protein gene expression in the adrenal gland of twin fetal sheep during late gestation.

    abstract：:We have previously demonstrated that maternal undernutrition during either the 'periconceptional' (i.e. from 60 days (d) before until 7 d after mating) or 'gestational' periods (i.e. from 8 d after mating until the end of pregnancy) have differential effects on the subsequent development of the hypothalamo-pituitary-a...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(02)00256-3

    authors： Edwards LJ,Bryce AE,Coulter CL,McMillen IC

    更新日期：2002-10-31 00:00:00

  • Maternal lipopolysaccharide exposure results in glucose metabolism disorders and sex hormone imbalance in male offspring.

    abstract：:An adverse intrauterine environment may be an important factor contributing to the development of type 2 diabetes in later life. The present study investigated the longitudinal effects of maternal lipopolysaccharide (LPS) exposure during the third trimester on glucose metabolism and sex hormone balance in the offsprin...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.03.019

    authors： Zhao M,Yuan L,Yuan MM,Huang LL,Su C,Chen YH,Yang YY,Hu Y,Xu DX

    更新日期：2018-10-15 00:00:00

  • Stanniocalcin-1 and -2 promote angiogenic sprouting in HUVECs via VEGF/VEGFR2 and angiopoietin signaling pathways.

    abstract：:The members of stanniocalcins (STCs: STC-1 and STC-2) family are known to be involved in tumor progression and metastasis. Although current evidences suggest the involvement of STCs in vascular biology, the functional roles of STCs in angiogenesis have not yet been elucidated. The objective of this study was to deciph...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2013.04.024

    authors： Law AY,Wong CK

    更新日期：2013-07-15 00:00:00

  • Immunocytochemical localization of inhibin in rat and human reproductive tissues.

    abstract：:Recently, the structures of two forms of inhibin present in human follicular fluid were elucidated from the corresponding cDNA sequences. Using specific antisera generated against the alpha-chain common to both forms, we have examined the cellular localization of inhibin in the male and female rat gonads and in human ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(87)90162-6

    authors： Merchenthaler I,Culler MD,Petrusz P,Negro-Vilar A

    更新日期：1987-12-01 00:00:00

  • Functional neuroanatomy of thyroid hormone feedback in the human hypothalamus and pituitary gland.

    abstract：:A major change in thyroid setpoint regulation occurs in various clinical conditions such as critical illness and psychiatric disorders. As a first step towards identifying determinants of these setpoint changes, we have studied the distribution and expression of thyroid hormone receptor (TR) isoforms, type 2 and type ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2006.03.042

    authors： Fliers E,Unmehopa UA,Alkemade A

    更新日期：2006-06-07 00:00:00

  • Characterization of nuclear triiodothyronine (T3) and tetraiodothyronine (T4) binding in developing brain tissue.

    abstract：:The main objective of this study was to characterize nuclear T3 and T4 binding in the developing rat brain. More specifically, we sought to determine (a) whether T3 and T4 bind to the same nuclear receptor, (b) whether there are multiple forms of nuclear T3 or T4 receptors, and (c) whether the above parameters are sim...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(83)90158-2

    authors： Margarity M,Matsokis N,Valcana T

    更新日期：1983-08-01 00:00:00

  • Empagliflozin attenuates ischemia and reperfusion injury through LKB1/AMPK signaling pathway.

    abstract：:The beneficial effects of empagliflozin (EMPA) on cardiac functions during ischemia and reperfusion were characterized. The contractile functions of isolated cardiomyocytes from adult C57BL/6J mice were determined with IonOptix SoftEdgeMyoCam system. The mitochondrial superoxide production was measured by MitoSOX fluo...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.110642

    authors： Lu Q,Liu J,Li X,Sun X,Zhang J,Ren D,Tong N,Li J

    更新日期：2020-02-05 00:00:00

  • Prenatal nicotine exposure and the programming of metabolic and cardiovascular disorders.

    abstract：:Presently, a growing interest is focused on the origins of the "Metabolic Syndrome", a cluster of several metabolic disorders linking obesity, dyslipidemia, hypertension and type 2 diabetes mellitus. Clearly, genetic predisposition and deleterious lifestyle, including low physical activity and hypercaloric alimentatio...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2009.02.026

    authors： Somm E,Schwitzgebel VM,Vauthay DM,Aubert ML,Hüppi PS

    更新日期：2009-05-25 00:00:00

  • In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia.

    abstract：:The food industry is moving towards the use of natural sweeteners such as those produced by Stevia rebaudiana due to the number of health and safety concerns surrounding artificial sweeteners. Despite the fact that these sweeteners are natural; they cannot be assumed safe. Steviol glycosides have a steroidal structure...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.03.005

    authors： Shannon M,Rehfeld A,Frizzell C,Livingstone C,McGonagle C,Skakkebaek NE,Wielogórska E,Connolly L

    更新日期：2016-05-15 00:00:00

  • Differential interaction of antiestrogens with cytosol estrogen receptors.

    abstract：:Interaction of tamoxifen, trioxifene and LY117018 with cytosol-estrogen receptors from immature rat uteri was compared. Determination of relative binding affinity (RBA) by competition with [3H] estradiol under various assay conditions revealed that the RBA of LY117018 increased with temperature while that of trioxifen...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(81)90105-2

    authors： Black LJ,Jones CD,Goode RL

    更新日期：1981-04-01 00:00:00

  • Isolation, characterisation and tissue localisation of an N-terminal-truncated variant of fibroblast growth factor.

    abstract：:An additional form of basic fibroblast growth factor (FGF), possessing full biological activity, has been isolated from bovine pituitary extracts. This polypeptide mitogen exhibits an apparent molecular weight of 17,500 by SDS-PAGE and lacks the first four N-terminal amino acid residues (Pro-Ala-Leu-Pro) of a previous...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(87)90028-1

    authors： Bertolini J,Hearn MT

    更新日期：1987-06-01 00:00:00

  • Molecular cloning and endometrial expression of porcine high density lipoprotein receptor SR-BI during the estrous cycle and early pregnancy.

    abstract：:During rapid development of the fetus, levels of high density lipoprotein (HDL) are elevated in pregnant women. The receptor for HDL, scavenger receptor class B type I (SR-BI), mediates selective cholesteryl ester uptake and is highly expressed in the human placenta. Because of the rapid growth of uterus during early ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2004.04.008

    authors： Kim JG,Vallet JL,Nonneman D,Christenson RK

    更新日期：2004-07-30 00:00:00

  • Progesterone and subsequent polar metabolites are essential for completion of the first meiotic division in amphibian oocytes.

    abstract：:We find that completion of the first meiotic division in Rana pipiens oocytes requires the sequential action of at least two steroids: progesterone and one or more subsequent polar metabolites of progesterone. Progesterone binding in vitro to oocyte surface receptors increases during the first 4-5h of exposure, follow...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2008.05.013

    authors： Kostellow AB,Morrill GA

    更新日期：2008-09-10 00:00:00

  • Expression of imprinted genes in human preimplantation development.

    abstract：:Imprinted gene expression in preimplantation development has been extensively studied in the mouse. Different imprinted genes vary in their time of onset of expression and also in the timing and tissue-specificity of mono-allelic expression. We have surveyed a range of imprinted genes for expression, and mono-allelic ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(01)00575-5

    authors： Monk M,Salpekar A

    更新日期：2001-10-22 00:00:00

  • Induction of insulin-like growth factor 1 splice forms by subfragments of myofibrillar proteins.

    abstract：:Expression of insulin-like growth factor 1 (IGF-1) mRNAs splice forms was recently shown to be stimulated by myofibrillar proteins released from the damaged muscle. In this study, we report that individual subfragments of titin and myomesin composed of Fn type III and Ig-like domains can activate expression of two IGF...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2014.08.010

    authors： Kravchenko IV,Furalyov VA,Chatziefthimiou S,Wilmanns M,Popov VO

    更新日期：2015-01-05 00:00:00

  • Hypothalamic mitochondrial abnormalities occur downstream of inflammation in diet-induced obesity.

    abstract：:Hypothalamic dysfunction is a common feature of experimental obesity. Studies have identified at least three mechanisms involved in the development of hypothalamic neuronal defects in diet-induced obesity: i, inflammation; ii, endoplasmic reticulum stress; and iii, mitochondrial abnormalities. However, which of these ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2017.07.029

    authors： Carraro RS,Souza GF,Solon C,Razolli DS,Chausse B,Barbizan R,Victorio SC,Velloso LA

    更新日期：2018-01-15 00:00:00

  • Estrogen receptor mutations.

    abstract：:The purpose of this paper is to review potential novel functional pathways by which estradiol and estrogenic compounds elicit biological responses in mammals. We will limit our approach to those novel functions suggested by phenotypes associated with estrogen receptor-alpha (ER alpha) gene mutations and polymorphisms....

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(98)00170-1

    authors： Taylor JA,Lewis KJ,Lubahn DB

    更新日期：1998-10-25 00:00:00

  • Intratesticular regulation of testosterone secretion: comparison of the effects and interactions of hCG, an LHRH agonist and testicular interstitial fluid on Leydig cell testosterone secretion in vitro.

    abstract：:The stimulatory effects on Leydig cell testosterone secretion of a polypeptide(s) factor present in testicular interstitial fluid (IF) were compared with those of hCG and an LHRH agonist (LHRH-A). The actions of IF and LHRH-A were similar in showing (1) a delayed onset of action, (2) enhancement of testosterone produc...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(85)90029-2

    authors： Sharpe RM

    更新日期：1985-07-01 00:00:00

  • Comparison of steroid and LH-RH effects on the responsiveness of hemipituitary glands and dispersed pituitary cells.

    abstract：:We have carried out further in vitro studies on the priming effect of LH-RH and the effect of steroids on pituitary responsiveness to LH-RH. In hemipituitary glands, the priming effect could be elicited only once within an 11-h period and was found to diminish significantly with time after the first exposure to LH-RH....

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(81)90003-4

    authors： Speight A,Fink G

    更新日期：1981-12-01 00:00:00

  • Auto-regulation of estrogen receptor subtypes and gene expression profiling of 17beta-estradiol action in the neuroendocrine axis of male goldfish.

    abstract：:Auto-regulation of the three goldfish estrogen receptor (ER) subtypes was examined simultaneously in multiple tissues, in relation to mRNA levels of liver vitellogenin (VTG) and brain transcripts. Male goldfish were implanted with a silastic implant containing either no steroid or 17beta-estradiol (E2) (100 microg/g b...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2007.10.013

    authors： Marlatt VL,Martyniuk CJ,Zhang D,Xiong H,Watt J,Xia X,Moon T,Trudeau VL

    更新日期：2008-02-13 00:00:00

  • Effect of exogenous chicken growth hormone (cGH) administration on insulin-like growth factor-I (IGF-I) gene expression in domestic fowl.

    abstract：:The effects of chicken growth hormone (cGH) infusion on insulin-like growth factor (IGF-I) gene expression in rapidly-growing, meat-type chickens was investigated. Chicken GH was infused either continuously or in a pulsatile fashion to 8-week-old birds during a 7-day period. Following cGH infusion, both IGF-I peptide ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)96796-k

    authors： Rosselot G,McMurtry JP,Vasilatos-Younken R,Czerwinski S

    更新日期：1995-10-30 00:00:00

  • Effects of thyroidectomy on heart and liver rat tRNAs: study of chromatographic and electrophoretic behaviour.

    abstract：:Modifications of tRNAs in various physiological or experimental conditions are well documented. We have compared isoacceptor tRNAs extracted from target organs (heart and liver) from thyroidectomized rats to those of control animals. Nine liver aminoacyl-tRNAs and eight heart aminoacyl-tRNAs from thyroidectomized and ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(83)90215-0

    authors： Kessous C,Befort JJ,Befort N,Benmiloud M

    更新日期：1983-02-01 00:00:00

  • Views on the co-evolution of the melanocortin-2 receptor, MRAPs, and the hypothalamus/pituitary/adrenal-interrenal axis.

    abstract：:A critical regulatory component of the hypothalamus/pituitary/adrenal axis (HPA) in mammals, reptiles and birds, and in the hypothalamus/pituitary/interrenal (HPI) axis of amphibians and teleosts (modern bony fishes) is the strict ligand selectivity of the melanocortin-2 receptor (MC2R). Tetrapod and teleost MC2R orth...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2014.12.022

    authors： Dores RM,Garcia Y

    更新日期：2015-06-15 00:00:00

  • D-glucose and L-leucine metabolism in neonatal and adult cultured rat pancreatic islets.

    abstract：:Neonatal and adult rat islets, cultured for 7-9 days in the presence of 10.5 mM D-glucose, were incubated for 120 min with either D-glucose (2.8 and 16.7 mM) or L-leucine (1.0 and 20.0 mM). The total and anaerobic rates of glycolysis, as judged respectively through the generation of 3H2O from D-[5-3H]glucose and 14C-l...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90045-a

    authors： Boschero AC,Bordin S,Sener A,Malaisse WJ

    更新日期：1990-10-01 00:00:00

  • Effect of growth hormone on surface charge and electrophoretic mobility of thymocytes from young and aged rats.

    abstract：:The electrophoretic mobility and surface charge of thymocytes was reduced on incubation of the cells with growth hormone in vitro. All cells in thymus were not found to be sensitive to the hormone. The proportion of cells whose surface charge was altered by the hormone was 65% in the thymocytes from 5-week-old rats. T...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(75)90044-1

    authors： Hanjan SN,Talwar GP

    更新日期：1975-09-01 00:00:00

  • The effects of hormones and prostaglandins on the calcium pools in cultured myocardial cells.

    abstract：:Determinations of the calcium pools in myocardial cells in vitro have shown the existence of at least three pools of exchangeable calcium. Epinephrine and glucagon were found to produce significant increases in the size of the two slower exchanging pools. Prostaglandins E1 and F1alpha also increased significantly calc...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(76)90017-4

    authors： Moura AM,Simpkins H

    更新日期：1976-10-01 00:00:00

  • Effects of ACTH administration on zonation of the guinea pig adrenal cortex.

    abstract：:Experiments were done to determine the actions of ACTH on the morphologic and functional characteristics of the zona fasciculata (ZF) and zona reticularis (ZR) in the guinea pig adrenal cortex. In control guinea pigs, a number of morphologic differences distinguished the ZF from the ZR, including the presence of far m...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(98)00189-0

    authors： Yuan BB,Tchao R,Funae Y,Voigt JM,Colby HD

    更新日期：1998-11-25 00:00:00

  • Similar metabolic responses to calorie restriction in lean and obese Zucker rats.

    abstract：:Calorie restriction (CR), which is thought to be largely dependent on the neuroendocrine system modulated by insulin/insulin-like growth factor-I (IGF-I) and leptin signaling, decreases morbidity and increases lifespan in many organisms. To elucidate whether insulin and leptin sensitivities are indispensable in the me...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2009.05.001

    authors： Chiba T,Komatsu T,Nakayama M,Adachi T,Tamashiro Y,Hayashi H,Yamaza H,Higami Y,Shimokawa I

    更新日期：2009-10-15 00:00:00

  • Insulin-induced tyrosine phosphorylation of Shc in liver, muscle and adipose tissue of insulin resistant rats.

    abstract：:Insulin stimulates rapid tyrosine phosphorylation of the protein Shc, which subsequently binds to Grb2, resulting in the activation of a complex mitogenic signaling network. In this study, we examined the levels of Shc protein, its phosphorylation state and Shc-Grb2 association in liver, muscle and adipose tissue befo...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(99)00137-9

    authors： Páez-Espinosa EV,Rocha EM,Velloso LA,Boschero AC,Saad MJ

    更新日期：1999-10-25 00:00:00

  • The intranuclear distribution of rat uterine estrogen receptors determined after nuclease treatment and chromatin fractionation.

    abstract：:The intranuclear locations at which rat uterine estrogen receptors interact with chromatin have been probed using digestions performed with DNAase I and micrococcal nuclease. Exposure to nuclease has been controlled to effect limited to extensive digestion of nuclear DNA under conditions which maintain the integrity o...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(82)90017-x

    authors： Pavlik EJ,Katzenellenbogen BS

    更新日期：1982-04-01 00:00:00