Abstract：
:An additional form of basic fibroblast growth factor (FGF), possessing full biological activity, has been isolated from bovine pituitary extracts. This polypeptide mitogen exhibits an apparent molecular weight of 17,500 by SDS-PAGE and lacks the first four N-terminal amino acid residues (Pro-Ala-Leu-Pro) of a previously described FGF preparation. Polyclonal antibodies have been raised to this FGF variant and used to examine the distribution of immunoreactive FGF species in rat and bovine tissues by immunoblot procedures. In extracts of rat pituitary, kidney, spleen and liver tissues several higher molecular weight species were detected with immunoreactive 70 kDa and 27 kDa protein bands predominating whilst a 32 kDa immunoreactive protein was also present in the brain. In bovine tissues several immunoreactive proteins with molecular weights greater than 17,500 were also observed in the brain, pituitary, adrenal, ovary and serum with these immunoblot procedures. With bovine pituitary extracts, six immunoblot bands corresponding to immunoreactive proteins of molecular weights 80,000-14,200 were observed, whilst with the brain extracts four immunoblot bands and with serum eight immunoblot bands ranging from 150,000 to 14,200 were detected. With non-immune rabbit serum, no polypeptides/proteins of corresponding molecular weight could be detected with similar immunoblot procedures. The possible relationships of these immunoreactive proteins to FGF precursor forms at various stages of processing are described.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Bertolini J,Hearn MTdoi
10.1016/0303-7207(87)90028-1subject
Has Abstractpub_date
1987-06-01 00:00:00pages
187-99issue
3eissn
0303-7207issn
1872-8057journal_volume
51pub_type
杂志文章abstract：:This study describes a model for short-term culture of intact mouse follicles under serum-free conditions. Follicles were either obtained from immature mice receiving no ovarian stimulation (i.e. no eCG-primed protocol, group I) or from mice undergoing ovarian stimulation (i.e. eCG-primed protocol, group II). Follicle...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2003.11.030
更新日期：2004-04-15 00:00:00
abstract：:TRESK (TWIK-related spinal cord K(+) channel, KCNK18) belongs to the two-pore domain (K2P) background (leak) potassium channel family. Unlike other K2P channels, TRESK is activated by the calcium signal in heterologous expression systems. The activation is mediated by the calcium/calmodulin-dependent protein phosphata...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2011.11.009
更新日期：2012-04-28 00:00:00
abstract：:Sterol 14 alpha-demethylase (CYP51) is the single cytochrome P450 (CYP) required for sterol biosynthesis in different phyla, and it is the most widely distributed P450 gene family being found in all biological kingdoms. It catalyzes the first step following cyclization in sterol biosynthesis such as removal of the 14 ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2003.11.016
更新日期：2004-02-27 00:00:00
abstract：:The immune system and the neuroendocrine system are functionally interactive. Lymphocytes possess opioid receptors as well as growth hormone receptors (GHR) by which opioids and growth hormone (GH) may modulate immune functions. In this study, we have investigated the effects of morphine on [125I]hGH binding and GHR g...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(97)00197-4
更新日期：1997-12-12 00:00:00
abstract：:The role of cannabinoid receptor I (CBR-1) in the induction of decidualization was examined using decidual fibroblasts and human endometrial stromal cells as model systems. Decidual fibroblasts decidualized in vitro for 3 and 6 days in the presence of the CBR-1 agonist R(+)-WIN 55,212-2 mesylate (WIN, 0.1-10 microM) e...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2004.09.007
更新日期：2005-01-14 00:00:00
abstract：:The study aims to examine the effects of restraint, cold, and in combination of hypoxia on pituitary GH mRNA and hepatic IGF-I mRNA and its protein in rats, and the potential involvement of corticotropin-releasing factor receptor subtype 1 (CRFR1) and SS in mediating the effects of continual hypoxia. Continual or inte...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2005.07.005
更新日期：2005-10-20 00:00:00
abstract：:Recent developments in the regulation of testicular Leydig-cell function support the following conclusions. (1) Enzymic activities involved in steroid production in the testis are mainly localized in Leydig cells. The aromatase enzyme complex for oestrogen production appears to be localized in Leydig cells as well as ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0303-7207(81)90026-5
更新日期：1981-01-01 00:00:00
abstract：:The degradation products generated from A14 and B26 125I-labelled insulins in liver endosomes in vivo and in vitro have been isolated by high-performance liquid chromatography and cleavages in the B chain have been identified by automated radiosequence analysis. In rats sacrificed various times after injection of each...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(90)90142-u
更新日期：1990-09-10 00:00:00
abstract：:The expression of the gene coding for thyroperoxydase, the main enzyme involved in the synthesis of the thyroid hormones, is controlled by thyroid stimulating hormone. In vitro transcription assays performed on nuclei isolated from dog thyroid cells in primary culture showed that this control is at the transcription l...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(88)90184-0
更新日期：1988-12-01 00:00:00
abstract：:The aim of this study was to investigate whether short-term treatment with nitric oxide donors could mimic cytokine inhibition of insulin secretion. We tested the nitric oxide generating compounds 3-morpholinosydnonimine (SIN-1), S-nitroso-N-penicillamine (SNAP), S-nitrosoglutathione and hydroxylamine for their abilit...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(94)90093-0
更新日期：1994-06-01 00:00:00
abstract：:Exploitation of estrogen's vasculoprotective properties in drug design is difficult due to its adverse effects on endometrium and breast. Selective estrogen receptor modulators (SERM) act as estrogen agonists in some tissues but are anti-estrogenic in others. We investigate here whether tamoxifen, raloxifene, and two ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2004.08.004
更新日期：2004-11-30 00:00:00
abstract：:Podocyte apoptosis in glomerular lesions has been found to have a dominant role in the progression of diabetic nephropathy. The present research aimed to explore the beneficial effect of icariin on diabetic podocytes by interfering in the process of apoptosis. Podocyte apoptosis was significantly exacerbated after hig...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2018.01.014
更新日期：2018-09-15 00:00:00
abstract：:Mice carrying an RGS-insensitive Gαi2 mutation display growth retardation early after birth. Although the growth hormone (GH)-axis is a key endocrine modulator of postnatal growth, its functional state in these mice has not been characterized. The present study was undertaken to address this issue. Results revealed th...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2020.111098
更新日期：2021-02-05 00:00:00
abstract：:Acid-labile subunit (ALS) deficiency (ACLSD) constitutes the first monogenic defect involving a member of the Insulin-like Growth Factor (IGF) binding protein system. The lack of ALS completely disrupts the circulating IGF system. Autocrine/paracrine action of local produced IGF-I could explain the mild effect on grow...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2020.111006
更新日期：2020-12-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2019.110582
更新日期：2019-12-01 00:00:00
abstract：:The concept of Developmental Origins of Health and Disease (DOHaD) states that exposure to malnutrition early in life increase the incidence of non-communicable chronic diseases throughout the lifespan. In this study, a reduction in serum testosterone and an increase in estrogen levels were shown in older rats born to...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2020.111148
更新日期：2020-12-31 00:00:00
abstract：:Our previous studies have provided evidence for the existence of an intrinsic renin-angiotensin system (RAS) in the rat pancreas, which may play a role in the regulation of pancreatic microcirculation and ductal secretion. Such a pancreatic RAS has recently shown to be activated by chronic hypoxia. The activation of a...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(00)00275-6
更新日期：2000-08-30 00:00:00
abstract：:The signal transducers and activators of transcriptions (Stats) are central mediators of cytokine responses especially in hematopoietic cells. The detailed molecular mechanisms of Stat activation, particularly the role of post-translational modifications and co-operation with cellular transcription factors are subject...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(01)00588-3
更新日期：2001-10-25 00:00:00
abstract：:The actions of ACTH to regulate the synthesis of the various enzymes involved in steroid hormone biosynthesis have been studied using bovine adrenocortical cells in monolayer culture. ACTH causes an increase in the synthesis of both the mitochondrial and the microsomal forms of cytochrome P-450 involved in steroid hor...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0303-7207(85)90123-6
更新日期：1985-02-01 00:00:00
abstract：:Microcirculation maintenance is associated with microRNAs. Nevertheless, the role of microRNAs induced by training in preventing dexamethasone (DEX)-induced microvascular rarefaction remains unknown. The study aim was to investigate if training-induced microRNAs are able to improve microcirculation proteins and preven...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2020.110732
更新日期：2020-04-05 00:00:00
abstract：:Berberine has been shown to have insulin-sensitizing effect, but the molecular mechanism underlying remains elusive. In this work, we investigated the effect of berberine on insulin-induced signal transduction and glucose uptake in both insulin-sensitive and insulin-resistant rat skeletal muscle cells. Berberine incre...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2009.12.027
更新日期：2010-04-12 00:00:00
abstract：:In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarray-determined transcriptome was...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2017.05.018
更新日期：2017-09-05 00:00:00
abstract：:The relationship between ATP-induced uptake of 45Ca2+ and the ATP-induced changes in [Ca2+]i was investigated in rat FRTL-5 thyroid cells. Addition of 1 microCi 45Ca2+/ml together with ATP induced a time- and dose-dependent increase in uptake of 45Ca2+, the uptake being still significantly above control after 30 min. ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(91)90105-2
更新日期：1991-08-01 00:00:00
abstract：:The synthesis and phosphorylation of H1 histones were studied in organ cultures of midpregnant mouse mammary glands exposed to various combinations of insulin, cortisol and prolactin over a 48-h period. The synthesis of specific H1 histone subtypes was changed only when all three hormones were present, and the effect ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(87)90137-7
更新日期：1987-11-01 00:00:00
abstract：:Inflammation causes male hypogonadism. Several inflammatory cytokines, including interleukin 6 (IL-6), are released into the blood and may suppress Leydig cell development. The objective of the present study was to investigate whether IL-6 affected the proliferation and differentiation of rat stem Leydig cells. Leydig...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2017.11.016
更新日期：2018-09-05 00:00:00
abstract：:The first exon of the human androgen receptor (AR) contains a translated CAG (poly-glutamine) repeat. The repeat length is polymorphic in the normal population ranging from 8 to 35 repeats. Expansions to over 40 repeats lead to spinal bulbar muscular atrophy (SBMA), a late onset neurodegenerative disease. The repeat i...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0303-7207(02)00104-1
更新日期：2002-07-31 00:00:00
abstract：:The binding of triiodothyronine (T3) to tadpole liver, intestine, and tail fin nuclei was consistent with a single class of binding sites. The KD for the binding of T3 to isolated nuclei from the liver (1.02 nM), intestine (1.40 nM), and tail fin (0.831 nM) nuclei were not significantly different. The number of bindin...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(87)90106-7
更新日期：1987-07-01 00:00:00
abstract：:Glucose transport activity ([3H]D-glucose uptake) in liver sinusoidal membrane vesicles (SMVs) from hyperthyroid rats was significantly higher than that from euthyroid controls (2.1-times increase in V(max) with K(m) unchanged at approximately 18 mM), associated with increased GLUT2 expression. In contrast, glucose tr...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(97)04026-4
更新日期：1997-04-04 00:00:00
abstract：:Calcium homeostasis refers to the regulation of calcium ion concentration in the body. This concentration is tightly controlled by a stabilizing system consisting of calcium channels and calcium buffering proteins. Calcium homeostasis is crucial for cell survival. Various forms of cell death (e.g., necrosis and apopto...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2015.05.017
更新日期：2015-09-05 00:00:00
abstract：:We previously described an androgen receptor (AR) point mutation located in the DNA-binding domain (DBD), adjacent to another AR substitution. Both were observed in two unrelated families with male breast cancer (MBC) and partial androgen insensitivity syndrome. This work was designed to determine the potential role o...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(97)00072-5
更新日期：1997-06-20 00:00:00
