Abstract:
:The immune system and the neuroendocrine system are functionally interactive. Lymphocytes possess opioid receptors as well as growth hormone receptors (GHR) by which opioids and growth hormone (GH) may modulate immune functions. In this study, we have investigated the effects of morphine on [125I]hGH binding and GHR gene expression in human lymphoblastoid IM-9 cells. Northern blot analysis revealed significantly altered GHR mRNA levels after treatment of the cells with different concentrations of morphine for 12 h. Morphine at 10 microM increased the mRNA levels in a time-dependent biphasic manner, with maximum levels at 6 and 48 h. The receptor protein, measured by [125I]GH binding, showed a time-delayed increase compared with the GHR mRNA changes. Pretreatment with naloxone inhibited the action of morphine, suggesting involvement of classical opioid receptors. The present study demonstrates, for the first time, effects of morphine on GHR mRNA levels and [125I]GH binding in human cultured lymphocytes.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Henrohn D,Le Grevés P,Nyberg Fdoi
10.1016/s0303-7207(97)00197-4subject
Has Abstractpub_date
1997-12-12 00:00:00pages
147-52issue
2eissn
0303-7207issn
1872-8057pii
S0303-7207(97)00197-4journal_volume
135pub_type
杂志文章abstract::The pineal hormone melatonin modulates constitutive protein secretion from melanoma M2R cells. Nanomolar concentrations of melatonin inhibited protein secretion early after plating or at low cell density, but facilitated it late after plating or at high cell density. Inhibition by melatonin of adenylate cyclase is the...
journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1975-12-01 00:00:00
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journal_title:Molecular and cellular endocrinology
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