Abstract：
:Neonatal and adult rat islets, cultured for 7-9 days in the presence of 10.5 mM D-glucose, were incubated for 120 min with either D-glucose (2.8 and 16.7 mM) or L-leucine (1.0 and 20.0 mM). The total and anaerobic rates of glycolysis, as judged respectively through the generation of 3H2O from D-[5-3H]glucose and 14C-labelled lactate from D-[3,4-14C]glucose or D-[6-14C]glucose were higher in neonatal than adult islets, but increased to a lesser relative extent in neonatal than adult islets in response to a rise in hexose concentration. The flow through the pentose phosphate pathway, as judged from the difference between D-[1-14C]glucose and D-[6-14C]glucose oxidation was higher in neonatal than adult islets. The flow through the reaction catalyzed by pyruvate dehydrogenase, as judged from the oxidation of D-[3,4-14C]glucose, was lower in neonatal than adult islets incubated in the presence of 16.7 mM (but not 2.8 mM) D-glucose. The oxidation of acetyl residues relative to their generation rate, as judged from the ratio of D-[6-14C]glucose to D-[3,4-14C]glucose oxidation, was not affected by the hexose concentration whether in neonatal or adult islets, but was about twice higher in the latter than former islets. The rate of D-[6-14C]glucose oxidation was also higher in adult than neonatal islets, especially at the high concentration of D-glucose. In both neonatal and adult islets, a rise in hexose concentration stimulated preferentially the oxidation of D[3,4-14C]glucose or D-[6-14C]glucose relative to the utilization of D-[5-3H]glucose.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Boschero AC,Bordin S,Sener A,Malaisse WJdoi
10.1016/0303-7207(90)90045-asubject
Has Abstractpub_date
1990-10-01 00:00:00pages
63-71issue
1eissn
0303-7207issn
1872-8057pii
0303-7207(90)90045-Ajournal_volume
73pub_type
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