当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Dehydroepiandrosterone (DHEA) treatment in vitro inhibits adipogenesis in human omental but not subcutaneous adipose tissue.

Dehydroepiandrosterone (DHEA) treatment in vitro inhibits adipogenesis in human omental but not subcutaneous adipose tissue.

Abstract：

:Dehydroepiandrosterone (DHEA), a precursor sex steroid, circulates in sulphated form (DHEAS). Serum DHEAS concentrations are inversely correlated with metabolic syndrome components and in vivo/in vitro studies suggest a role in modulating adipose mass. To investigate further, we assessed the in vitro biological effect of DHEA in white (3T3-L1) and brown (PAZ6) preadipocyte cell lines and human primary preadipocytes. DHEA (from 10(-8)M) caused concentration-dependent proliferation inhibition of 3T3-L1 and PAZ6 preadipocytes. Cell cycle analysis demonstrated unaltered apoptosis but indicated blockade at G1/S or G2/M in 3T3-L1 and PAZ6, respectively. Preadipocyte cell-line adipogenesis was not affected. In human primary subcutaneous and omental preadipocytes, DHEA significantly inhibited proliferation from 10(-8)M. DHEA 10(-7)M had opposing effects on adipogenesis in the two fat depots. Subcutaneous preadipocyte differentiation was unaffected or increased whereas omental preadipocytes showed significantly reduced adipogenesis. We conclude that DHEA exerts fat depot-specific differences which modulate body composition by limiting omental fat production.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Rice SP,Zhang L,Grennan-Jones F,Agarwal N,Lewis MD,Rees DA,Ludgate M

doi

10.1016/j.mce.2010.02.017

subject

Has Abstract

pub_date

2010-05-14 00:00:00

pages

51-7

issue

1-2

eissn

0303-7207

issn

1872-8057

pii

S0303-7207(10)00093-6

journal_volume

320

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Autoradiographic localization of beta-adrenergic receptors in rat oviduct.

    abstract：:Catecholamines are known to have an inhibitory effect on oviductal smooth musculature by changing the adrenergic receptors activity. In order to further investigate the role of epinephrine and/or norepinephrine in oviduct, the distribution of beta-adrenergic receptors has been studied in the rat oviduct, using in vitr...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(88)90123-2

    authors： Tolszczuk M,Pelletier G

    更新日期：1988-11-01 00:00:00

  • Discordant expression of insulin-like growth factors and their receptor messenger ribonucleic acids in endometrial carcinomas relative to normal endometrium.

    abstract：:The inappropriate expressions of insulin-like growth factors (IGF-I and II) and IGF-I receptor (IGF-IR) are implicated in the malignant growth of many cancers. To determine changes, if any, in the levels of expression of IGFs and IGF receptor genes in neoplastic endometrium, relative to normal endometrium, the mRNA le...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(99)00092-1

    authors： Roy RN,Gerulath AH,Cecutti A,Bhavnani BR

    更新日期：1999-07-20 00:00:00

  • Differential regulation of corticotropin-releasing factor receptor type 1 (CRF1 receptor) mRNA via protein kinase A and mitogen-activated protein kinase pathways in rat anterior pituitary cells.

    abstract：:Corticotropin-releasing factor (CRF) receptor type 1 (CRF(1) receptor) mRNA levels are down-regulated by CRF via the cyclic AMP-protein kinase A (PKA) pathway. In this study, we focused on the involvement of both the mitogen-activated protein (MAP) kinase pathway and PKA in this regulation. Real-time PCR (RT-PCR) reve...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2005.09.010

    authors： Moriyama T,Kageyama K,Kasagi Y,Iwasaki Y,Nigawara T,Sakihara S,Suda T

    更新日期：2005-11-24 00:00:00

  • Inhibition of FSH action on granulosa cells by low molecular weight components of follicular fluid.

    abstract：:Immature female rats were injected with a single dose (10 IU) of pregnant mare serum gonadotropin to induce growth and maturation of ovarian follicles. Using such ovaries as a model, we tested the effects of low molecular weight subfractions of charcoal-absorbed bovine follicular fluid (FF-c) on (a) radioiodinated hum...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(82)90086-7

    authors： Fletcher PW,Dias JA,Sanzo MA,Reichert LE Jr

    更新日期：1982-03-01 00:00:00

  • D-glucose and L-leucine metabolism in neonatal and adult cultured rat pancreatic islets.

    abstract：:Neonatal and adult rat islets, cultured for 7-9 days in the presence of 10.5 mM D-glucose, were incubated for 120 min with either D-glucose (2.8 and 16.7 mM) or L-leucine (1.0 and 20.0 mM). The total and anaerobic rates of glycolysis, as judged respectively through the generation of 3H2O from D-[5-3H]glucose and 14C-l...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90045-a

    authors： Boschero AC,Bordin S,Sener A,Malaisse WJ

    更新日期：1990-10-01 00:00:00

  • Designing a new generation of anti-hCG vaccines for cancer therapy.

    abstract：:The heterodimeric 'pregnancy-specific' hormone human chorionic gonadotropin (hCG) has been used as the basis for a contraceptive vaccine. More recently, the observation that hCG, particularly in the form of the beta-chain expressed in the absence of alpha-chain, is aberrantly expressed in a number of different tumors ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2006.02.018

    authors： Delves PJ,Iles RK,Roitt IM,Lund T

    更新日期：2007-01-02 00:00:00

  • Role of epididymal receptor HE6 in the regulation of sperm microenvironment.

    abstract：:HE6 (GPR64) is a highly conserved, tissue-specific heptahelical receptor of the human epididymis. The seven transmembrane (TM7) domains are a hallmark of G-protein-coupled receptors (GPCRs) which have a proven history of being excellent therapeutic targets. Of all currently marketed drugs, >30% are modulators of speci...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2005.12.023

    authors： Kirchhoff C,Obermann H,Behnen M,Davies B

    更新日期：2006-05-16 00:00:00

  • Insulin and insulin-like growth factor-1 can modulate the phosphoinositide-3-kinase/Akt/FoxO1 pathway in SZ95 sebocytes in vitro.

    abstract：:A recent hypothesis suggests that a high glycaemic load diet-associated increase of insulin-like growth factor-1 (IGF-1) and insulin may promote acne by reducing nuclear localization of the forkhead box-O1 (FoxO1) transcription factor via activation of the phosphoinositide-3-kinase (PI3K)/Akt pathway. Using SZ95 seboc...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.08.001

    authors： Mirdamadi Y,Thielitz A,Wiede A,Goihl A,Papakonstantinou E,Hartig R,Zouboulis CC,Reinhold D,Simeoni L,Bommhardt U,Quist S,Gollnick H

    更新日期：2015-11-05 00:00:00

  • Characterization of complementary deoxyribonucleic acids encoding preprogalanin and its alternative splice variants in the goldfish.

    abstract：:This paper reports the identification of five preprogalanin complementary deoxyribonucleic acids encoding preprogalanin peptides in the goldfish. Preprogalanin 1A, 1B and 1C are encoded in galanin gene 1 and 2A and 2B are encoded in gene 2. Preprogalanin 1B and 2B have a 24 amino acids insert in the mature peptide reg...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(02)00301-5

    authors： Unniappan S,Lin X,Peter RE

    更新日期：2003-02-28 00:00:00

  • The structure of cDNA clones encoding the aromatase P-450 isolated from a rat Leydig cell tumor line demonstrates differential processing of aromatase mRNA in rat ovary and a neoplastic cell line.

    abstract：:The conversion of androgens to estrogens is catalyzed by a complex of enzymes that includes a specific cytochrome P-450 aromatase (P-450arom). In this paper we describe the high level expression of aromatase activity in the rat Leydig cell tumor line, R2C. We also report the isolation of cDNA clones encoding the rat a...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90056-e

    authors： Lephart ED,Peterson KG,Noble JF,George FW,McPhaul MJ

    更新日期：1990-03-26 00:00:00

  • Modulation of 1,25(OH)2D3-induced osteocalcin synthesis in human osteosarcoma cells by other steroidal hormones.

    abstract：:We have previously shown that osteocalcin synthesis is readily induced by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in MG-63 human osteosarcoma cells (Mahonen et al. (1990) Biochim. Biophys. Acta 1048, 30-37). In the present study, the regulation of osteocalcin synthesis by other hormones of the steroid-thyroid hormone f...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(91)90269-x

    authors： Pirskanen A,Mahonen A,Mäenpää PH

    更新日期：1991-04-01 00:00:00

  • Eppin: an effective target for male contraception.

    abstract：:Eppin (epididymal protease inhibitor) is a member of the whey acidic protein (WAP)-type four-disulfide core (WFDC) gene family. This study provides updated information on Eppin and the Eppin-like genes within the Eppin cluster on human chromosome 20. A virtual structural model of the Eppin protein demonstrates that th...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2005.12.039

    authors： O'Rand MG,Widgren EE,Wang Z,Richardson RT

    更新日期：2006-05-16 00:00:00

  • Genetics and phenomics of hypothyroidism and thyroid dys- and agenesis due to PAX8 and TTF1 mutations.

    abstract：:Thyroid dysgenesis (TD) is the most common cause of congenital hypothyroidism (CH), a relatively frequent endocrine disease in newborns (1 in 3000-4000 live births). TD is a defect in the organogenesis of the gland resulting in hypoplastic, ectopic or absent-thyroid gland. TD is usually sporadic but mutations in trans...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2010.03.009

    authors： Montanelli L,Tonacchera M

    更新日期：2010-06-30 00:00:00

  • Impairment of ATP-induced Ca2+ -signalling in human thyroid cancer cells.

    abstract：:Extracellular nucleotides like ATP that activate the Ca2+ -phosphatidylinositol (PI) signalling pathway have been suggested to participate in the regulation of normal human thyroid function. We examined, whether P2y-purinergic receptors are expressed on human thyroid cancer cells and whether post-receptor Ca2+ signall...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(97)00141-x

    authors： Schöfl C,Rössig L,Mader T,Börger J,Pötter E,von zur Mühlen A,Brabant G

    更新日期：1997-09-30 00:00:00

  • Regulation of function of the murine luteinizing hormone receptor promoter by cis- and trans-acting elements in mouse Leydig tumor cells.

    abstract：:The transcriptional activity of various lengths of the 5'-untranslated region (UTR) of the murine LH receptor (R) gene were studied using the luciferase reporter gene in transiently transfected mouse Leydig tumor cells (mLTC-1). Chinese hamster ovarian (CHO) and HeLa cells were used as controls. The basal transcriptio...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(96)03815-4

    authors： El-Hefnawy T,Krawczyk Z,Nikula H,Viherä I,Huhtaniemi I

    更新日期：1996-05-31 00:00:00

  • Glucocorticoid receptor knock down reveals a similar apoptotic threshold but differing gene regulation patterns in T-cell and pre-B-cell acute lymphoblastic leukemia.

    abstract：:Glucocorticoids (GCs) are used in combination therapy for treating acute lymphoblastic leukemia (ALL). In T-cell (CEM-C7) and pre-B-cell (697) ALL cell lines, dexamethasone (Dex) treatment causes an auto-upregulation of glucocorticoid receptor (GR) mRNA transcripts and protein. We hypothesized that there is a threshol...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2010.02.014

    authors： Schwartz JR,Sarvaiya PJ,Vedeckis WV

    更新日期：2010-05-14 00:00:00

  • Molecular regulation of LHCGR expression by miR-122 during follicle growth in the rat ovary.

    abstract：:We have previously reported that LHCGR expression in the ovary is regulated through a post-transcriptional mechanism involving an mRNA binding protein designated as LRBP, which is regulated, at least in part, by a non-coding RNA, miR-122. Our present study examined the regulatory role of miR-122 in FSH-induced LHCGR e...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.12.002

    authors： Menon B,Gulappa T,Menon KM

    更新日期：2017-02-15 00:00:00

  • Compound heterozygous DUOX2 gene mutations (c.2335-1G>C/c.3264_3267delCAGC) associated with congenital hypothyroidism. Characterization of complex cryptic splice sites by minigene analysis.

    abstract：:Iodide Organification defects (IOD) represent 10% of cases of congenital hypothyroidism (CH) being the main genes affected that of TPO (thyroid peroxidase) and DUOX2 (dual oxidasa 2). From a patient with clinical and biochemical criteria suggestive with CH associated with IOD, TPO and DUOX2 genes were analyzed by mean...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.10.014

    authors： Belforte FS,Citterio CE,Testa G,Olcese MC,Sobrero G,Miras MB,Targovnik HM,Rivolta CM

    更新日期：2016-01-05 00:00:00

  • Phorbol ester- and luteinizing hormone-induced phosphorylation of membrane proteins in bovine luteal cells.

    abstract：:In this study we have investigated the protein phosphorylation pattern in the membrane fraction prepared from bovine luteal cells. The phosphorylation reaction was carried out in vitro, under defined conditions, using either [gamma-32P]ATP or [gamma-35S]ATP as the phosphate donor. The results obtained show that [gamma...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90018-4

    authors： Budnik LT,Mukhopadhyay AK

    更新日期：1990-03-05 00:00:00

  • Cyclic AMP-dependent and independent stimulations of ovarian steroidogenesis by brain factors in the blowfly, Phormia regina.

    abstract：:The involvement of cyclic-AMP (cAMP) as a potential second messenger in the neurohormonal control of ovarian steroidogenesis was investigated in the adult female blowfly Phormia regina. Individual measurements of ovarian cAMP concentrations and of ovarian biosynthesis of ecdysteroids, stimulated after a protein meal, ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(00)00312-9

    authors： Manière G,Vanhems E,Delbecque J

    更新日期：2000-10-25 00:00:00

  • Nicotinamide analogs and DNA-damaging agents deplete thyroid hormone receptor and c-erbA mRNA levels in pituitary GH1 cells.

    abstract：:Incubation of pituitary GH1 cells with N'-methylnicotinamide, nicotinamide and 3-acetylpyridine which inhibit nuclear ADP-ribosylation and/or the cellular concentration of its substrate NAD+ reduced the amount of nuclear thyroid hormone receptors in a time- and dose-dependent manner without altering the affinity of th...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(93)90264-k

    authors： Sanchez-Pacheco A,Perez P,Villa A,Pascual A,Aranda A

    更新日期：1993-02-01 00:00:00

  • Small molecule adiponectin receptor agonist GTDF protects against skeletal muscle atrophy.

    abstract：:Skeletal muscle atrophy is a debilitating response to several major diseases, muscle disuse and chronic steroid treatment for which currently no therapy is available. Since adiponectin signaling plays key roles in muscle energetics, we assessed if globular adiponectin (gAd) or the small molecule adiponectin mimetic 6-...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.09.013

    authors： Singh AK,Shree S,Chattopadhyay S,Kumar S,Gurjar A,Kushwaha S,Kumar H,Trivedi AK,Chattopadhyay N,Maurya R,Ramachandran R,Sanyal S

    更新日期：2017-01-05 00:00:00

  • Impact of endocrine-disrupting compounds (EDCs) on female reproductive health.

    abstract：:Evidence is accumulating that environmental chemicals (ECs) including endocrine-disrupting compounds (EDCs) can alter female reproductive development, fertility and onset of menopause. While not as clearly defined as in the male, this set of abnormalities may constitute an Ovarian Dysgenesis Syndrome with at least som...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2011.10.021

    authors： Fowler PA,Bellingham M,Sinclair KD,Evans NP,Pocar P,Fischer B,Schaedlich K,Schmidt JS,Amezaga MR,Bhattacharya S,Rhind SM,O'Shaughnessy PJ

    更新日期：2012-05-22 00:00:00

  • Elimination of adrenocortical apolipoprotein E production does not impact glucocorticoid output in wild-type mice.

    abstract：:Apolipoprotein E (APOE) deficient mice exhibit unexplained hypercorticosteronemia. Given that APOE is also produced locally within the adrenals, we evaluated the effect of adrenal-specific APOE deficiency on the glucocorticoid function. Hereto, one adrenal containing or lacking APOE was transplanted into adrenalectomi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.04.001

    authors： van der Sluis RJ,Depuydt MAC,Verwilligen RAF,Hoekstra M,Van Eck M

    更新日期：2019-06-15 00:00:00

  • The spatial and temporal regulation of the hormonal signal. Role of mitochondria in the formation of a protein complex required for the activation of cholesterol transport and steroids synthesis.

    abstract：:The mitochondria are critical for steroidogenesis since the ability of cholesterol to move into mitochondria to be available for cytochrome P450, CYP11A1, determines the efficacy of steroid production. Several proteins kinases, such as PKA, MEK and ERK which are essential to complete steroidogenesis, form a mitochondr...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2012.12.024

    authors： Poderoso C,Duarte A,Cooke M,Orlando U,Gottifredi V,Solano AR,Lemos JR,Podestá EJ

    更新日期：2013-05-22 00:00:00

  • Evaluating the effects on steroidogenesis of estragole and trans-anethole in a feto-placental co-culture model.

    abstract：:In this study, we determined whether estragole and its isomer trans-anethole interfered with feto-placental steroidogenesis in a human co-culture model composed of fetal-like adrenocortical (H295R) and placental trophoblast-like (BeWo) cells. Estragole and trans-anethole are considered the biologically active compound...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.110583

    authors： Yancu D,Vaillancourt C,Sanderson JT

    更新日期：2019-12-01 00:00:00

  • Isolation, characterisation and tissue localisation of an N-terminal-truncated variant of fibroblast growth factor.

    abstract：:An additional form of basic fibroblast growth factor (FGF), possessing full biological activity, has been isolated from bovine pituitary extracts. This polypeptide mitogen exhibits an apparent molecular weight of 17,500 by SDS-PAGE and lacks the first four N-terminal amino acid residues (Pro-Ala-Leu-Pro) of a previous...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(87)90028-1

    authors： Bertolini J,Hearn MT

    更新日期：1987-06-01 00:00:00

  • Effect of the TYK-2/STAT-3 pathway on lipid accumulation induced by mono-2-ethylhexyl phthalate.

    abstract：BACKGROUND:Mono-2-ethylhexyl phthalate (MEHP), an important metabolite of di (2-ethylhexyl) phthalate (DEHP), can induce lipid metabolic disorder. Previous studies have shown that MEHP promotes 3T3-L1 cell differentiation; however, the underlying mechanism is unclear. The present study was performed to investigate the ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.01.012

    authors： Qi W,Zhou L,Zhao T,Ding S,Xu Q,Han X,Zhao Y,Song X,Zhao T,Zhang X,Ye L

    更新日期：2019-03-15 00:00:00

  • Reduction of protein phosphatase 2A Cα promotes in vivo bone formation and adipocyte differentiation.

    abstract：:Serine/threonine protein phosphatase 2A (PP2A) regulates diverse physiological processes such as cell cycle, growth, apoptosis, and signal transduction. Previously, we demonstrated that silencing of the α-isoform of PP2A catalytic subunit (PP2A Cα) in osteoblasts accelerated osteoblast differentiation, whereas its ove...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2017.11.005

    authors： Yoshida K,Teramachi J,Uchibe K,Ikegame M,Qiu L,Yang D,Okamura H

    更新日期：2018-07-15 00:00:00

  • Role of ligand-dependent GR phosphorylation and half-life in determination of ligand-specific transcriptional activity.

    abstract：:A central question in glucocorticoid mechanism of action via the glucocorticoid receptor (GR) is what determines ligand-selective transcriptional responses. Using a panel of 12 GR ligands, we show that the extent of GR phosphorylation at S226 and S211, GR half-life and transcriptional response, occur in a ligand-selec...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2010.06.007

    authors： Avenant C,Ronacher K,Stubsrud E,Louw A,Hapgood JP

    更新日期：2010-10-07 00:00:00