Abstract：
:Sperm cryopreservation still represents a valuable clinical aid in the management of infertility. Its current principal indications include (1) donor sperm insemination; (2) freezing before cancer therapy to maintain reproductive capacity; (3) patient's convenience; and (4) because of the outstanding success with ICSI, even patients with different degrees of oligo-asthenoteratozoospermia can now be offered the use of frozen/thawed sperm for oocyte micromanipulation. Although sperm cryopreservation/thawing and results of insemination and IVF have been consistently good using donor semen, results of infertile men (with or without various degrees of oligoasthenoteratozoospermia) have yielded remarkably lower rates of survival and pregnancy. Freezing/thawing techniques have not been subjected to major changes in the last years, Furthermore, the exact nature of sperm cryodamage still remains to be elucidated. Various aspects of sperm freezing are revisited here (1) development of new technical approaches for cryopreservation; (2) analysis of the stimulatory effect of putative cryoprotectant additives; (3) the use of intrauterine insemination-ready processed samples; and (4) selection and optimization of end-points for analysis of cryodamage. It is expected that advances in such areas will improve significantly the cryopreservation/thawing outcome particularly as related to semen samples of subfertile men.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Oehninger S,Duru NK,Srisombut C,Morshedi Mdoi
10.1016/s0303-7207(00)00343-9keywords:
subject
Has Abstractpub_date
2000-11-27 00:00:00pages
3-10issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(00)00343-9journal_volume
169pub_type
杂志文章,评审abstract：:Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are widely recognized for their roles in ovulation and the support of early pregnancy. Aside from the timing of expression, however, the differences between LH and hCG have largely been overlooked in the clinical realm because of their similar molecular s...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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abstract：:The interaction between corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) is important in the regulation of adrenocorticotropin (ACTH) release from the anterior pituitary (AP). CRF exerts its effect on the AP by activating the adenylate cyclase (AC) complex whereas AVP increases the turnover of phosp...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(94)90232-1
更新日期：1994-05-01 00:00:00
abstract：:Receptor-mediated Ca2+ entry was investigated in fura-2-loaded thyroid FRTL-5 cells. Activation of protein kinase C (PKC) by phorbol myristate acetate (PMA) attenuated the ATP-induced increase in intracellular free Ca2+ ([Ca2+]i). In PKC down-regulated cells, the ATP-induced increase in [Ca2+]i was increased compared ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(93)90134-6
更新日期：1993-05-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(01)00588-3
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2011.07.036
更新日期：2012-01-02 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(91)90019-o
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(02)00301-5
更新日期：2003-02-28 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(87)90096-7
更新日期：1987-07-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2007.07.007
更新日期：2007-10-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(88)90184-0
更新日期：1988-12-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2010.01.032
更新日期：2010-05-14 00:00:00
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pub_type： 杂志文章
doi：10.1016/0303-7207(81)90072-1
更新日期：1981-08-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(03)00234-x
更新日期：2003-08-29 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2020-03-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2012.10.030
更新日期：2013-01-30 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2005.07.005
更新日期：2005-10-20 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(95)03578-u
更新日期：1995-07-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：1990-01-02 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(82)90017-x
更新日期：1982-04-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2018-09-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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更新日期：1991-05-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(89)90162-7
更新日期：1989-08-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2019-08-20 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2015.04.005
更新日期：2015-07-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(83)90031-x
更新日期：1983-07-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2013.12.008
更新日期：2014-03-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(79)90069-8
更新日期：1979-07-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2005.05.004
更新日期：2005-10-20 00:00:00
abstract：:Resistance to thyroid hormone can be due to heterozygous, dominant negative (DN) THRA (RTHα) or THRB (RTHβ) mutations, but the underlying mechanisms are incompletely understood. Here, we delineate the spatiotemporal expression of TH receptors (TRs) in zebrafish and generated morphants expressing equivalent amounts of ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2016.01.020
更新日期：2016-03-15 00:00:00
abstract：:Puberty is a time of striking changes in cognition and behavior. To indirectly assess the effects of puberty-related influences on the underlying neuroanatomy of these behavioral changes we will review and synthesize neuroimaging data from typically developing children and adolescents and from those with anomalous hor...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2006.04.016
更新日期：2006-07-25 00:00:00
