Abstract：
:Growth hormone (GH) secretion, evoked by either pituitary adenylate cyclase-activating polypeptide (PACAP) or dopamine (DA), is dependent on both voltage-sensitive calcium channels (VSCC) and cAMP signaling in goldfish. We further characterized the involvement of Ca2+ in evoked release by PACAP and DA, by examining the sensitivity of evoked GH release to perturbations of Ca2+ signaling. Both VSCC and calmodulin/calmodulin-dependent kinase are involved in PACAP signaling as had been shown for DA. In spite of this apparent dependence on VSCC, blockade of TMB-8 but not ryanodine-sensitive intracellular Ca2+ stores inhibited both PACAP- and DA-evoked GH release. Using sarcoplasmic/endoplasmic reticulum Ca-ATPases (SERCA) inhibitors, we found BHQ blocked, whereas thapsigargin (Tg) enhanced stimulated GH release, suggesting that Tg-sensitive SERCA may counteract these cAMP-mobilizing neuroendocrine regulators by sequestering [Ca2+]i. As GH secretion stimulated by two endogenous gonadotropin-releasing hormones is not affected by Tg, it appears that distinct multiple Ca2+ stores mediate the hormone releasing response to different neuroendocrine regulators.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Chang JP,Wong CJ,Davis PJ,Soetaert B,Fedorow C,Sawisky Gdoi
10.1016/s0303-7207(03)00234-xkeywords:
subject
Has Abstractpub_date
2003-08-29 00:00:00pages
63-74issue
1-2eissn
0303-7207issn
1872-8057pii
S030372070300234Xjournal_volume
206pub_type
杂志文章abstract：:Studies on the age-related decline of growth hormone (GH) release have ignored that the population of GH-producing cells (somatotropes) is heterogeneous. In aging male rats, centrifugation of dispersed pituitary cells in a density gradient yields two somatotrope subpopulations, i.e. low- (LD) and high-density (HD) cel...
journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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journal_title：Molecular and cellular endocrinology
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abstract：:Autodigestion of nuclei isolated from the androgen-dependent Shionogi mouse mammary carcinoma revealed the presence of endogenous endonuclease activity which was stimulated by Ca2+ and Mg2+ and generated a repeating pattern of DNA fragments with an average monomeric size of 179 base pairs. The nuclear concentration of...
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pub_type： 杂志文章,评审
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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更新日期：2011-12-05 00:00:00
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journal_title：Molecular and cellular endocrinology
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doi：10.1016/j.mce.2011.03.020
更新日期：2011-06-06 00:00:00
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更新日期：2020-01-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(88)90013-5
更新日期：1988-03-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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更新日期：2010-09-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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journal_title：Molecular and cellular endocrinology
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更新日期：2018-11-15 00:00:00
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更新日期：2011-04-30 00:00:00
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pub_type： 临床试验,杂志文章
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journal_title：Molecular and cellular endocrinology
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更新日期：2007-03-30 00:00:00
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journal_title：Molecular and cellular endocrinology
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更新日期：2003-12-30 00:00:00
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journal_title：Molecular and cellular endocrinology
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更新日期：2016-09-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(95)03592-u
更新日期：1995-08-11 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2010-02-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(96)03787-2
更新日期：1996-05-17 00:00:00
